key: cord-0941921-vuo9oh9k authors: Poisson, Tiffany M.; Pierone, Gerald title: Placental pathology and fetal demise at 35 weeks of gestation in a woman with SARS-CoV-2 infection: A case report date: 2021-01-28 journal: Case Rep Womens Health DOI: 10.1016/j.crwh.2021.e00289 sha: 46da2f52ad7492cffd5b00541bcb771bcb67d06d doc_id: 941921 cord_uid: vuo9oh9k The consequences of SARS-CoV-2 infection in pregnancy have not been well defined. However, there have been a number of reports of poor maternal and fetal outcomes worldwide. This report presents a case of stillbirth with associated placental pathology during week 35 in an otherwise healthy pregnant woman with SARS-CoV-2 infection. Placental findings in this case showed patchy acute chorionitis and diffuse infarction/villous necrosis of the placental parenchyma resulting in extensive vascular malperfusion. Fetal autopsy was most significant for placental findings and no congenital malformations were discovered. The findings in this case are consistent with reports in the literature of pathological placental changes associated with COVID-19. This case of fetal demise in a woman with confirmed SARS-CoV-2 infection without any other medical or obstetric disorders and no alternate cause suggests that fetal death can be an outcome of COVID-19 during pregnancy. This outcome was supported by the histopathological findings in the placenta. Continued research is imperative to confirm the findings in this case and multiple similar cases. Additionally, increased screening and collection of COVID-19 data specific to pregnant women and their fetuses and infants is needed to increase knowledge, support research efforts, and create guidelines for clinical practice that will prevent potential negative outcomes and loss of life. The consequences of SARS-CoV-2 infection in pregnancy have not been well defined. However, there have been a number of reports of poor maternal and fetal outcomes worldwide. This report presents a case of stillbirth with associated placental pathology during week 35 in an otherwise healthy pregnant woman with SARS-CoV-2 infection. Placental findings in this case showed patchy acute chorionitis and diffuse infarction/villous necrosis of the placental parenchyma resulting in extensive vascular malperfusion. Fetal autopsy was most significant for placental findings and no congenital malformations were discovered. The findings in this case are consistent with reports in the literature of pathological placental changes associated with COVID-19. This case of fetal demise in a woman with confirmed SARS-CoV-2 infection without any other medical or obstetric disorders and no alternate cause suggests that fetal death can be an outcome of COVID-19 during pregnancy. This outcome was supported by the histopathological findings in the placenta. Continued research is imperative to confirm the findings in this case and multiple similar cases. The global impact of the novel coronavirus (2019-nCoV, or SARS-CoV-2) has been well documented; however, studies and data to inform proper management of pregnant women and fetuses during the COVID-19 pandemic, though increasing recently, have been limited. There have been a number of reports of poor maternal and fetal outcomes worldwide, but few studies have directly linked these outcomes to COVID-19, likely due to poor data collection for pregnant women early on and the limited statistical power of smaller studies and observational reports available in the literature thus far. Though limited data exists regarding the effects of COVID-19 on fetal health and development, concern for and clinical reports of increased rates of miscarriage and stillbirth, growth restriction, and preterm births have been emerging since early in the pandemic (1) (2) (3) . More recently, reports from the CDC have shown stillbirth rates of 2.2%-3% in multistate surveillance studies (4) (5) . When compared to the average US stillbirth rate of <1% before the pandemic, this data trend is alarming (6) . Additionally, emerging evidence has suggested potential COVID-19-induced placental pathology with associated fetal death in some cases (1) (2) (7) (8) . The placental pathology in COVID-19 observed and discussed in five existing studies are also present in this case and confirmed with placental pathology study and fetal autopsy (7) (8) (9) (10) (11) . Interestingly, similarities in the clinical course and features for patients described in case reports by Richtmann et al. and Baud et al. J o u r n a l P r e -p r o o f Journal Pre-proof (nulliparous, otherwise healthy women with no pregnancy complications who were managed as outpatients with relatively mild forms of are also consistent in this case (1) (2) . We present a case of stillbirth with associated placental pathology during week 35 in an otherwise healthy pregnant woman with SARS-CoV-2 infection. A 31-year-old asymptomatic, healthy, white, nulliparous woman was well until week 32 of her pregnancy. Of note, this patient is an essential healthcare worker. She was practicing as a primary care clinician and actively caring for patients during the COVID-19 pandemic in the midst of widespread community transmission. The patient then developed fatigue, rhinorrhea, body aches, mild headache, sore throat, nausea and vomiting, diarrhea, cough, chills, and anosmia during her illness. She reported no fever, shortness of breath, or respiratory distress. Her illness had not required hospitalization. Positive RT-PCR for SARS-CoV-2 results were received using nasopharyngeal swabs collected on day 3 and 14 of the illness. Unfortunately, the patient was not evaluated during her illness and experienced a 27-day gap in prenatal care due to ill-advised hospital-specific clinical policies limiting care of pregnant women until complete resolution of symptoms and evidence of negative RT-PCR COVID-19 testing. The patient was evaluated at an outpatient obstetric clinic at 35 4/7 weeks of gestation for routine prenatal care after recent COVID-19 infection. She was afebrile, HR 103, BP 103/62, BMI 27.81, and physical exam was unremarkable except for discovery of absent fetal heart rate (FHR). There was no history of abdominal pain, vaginal bleeding, uterine contractions, or poor fetal movement. There was no report of fever, new illness, or injury. There were no comorbidities or pregnancy complications. Routine laboratory tests, genetic screenings, and all anomaly scans were normal. After diagnosis of intrauterine fetal demise via obstetric ultrasound the patient was admitted to labor and delivery. Continued positive RT-PCR results were received upon admission using a nasopharyngeal swab (COVID day 24). Physical exam was normal and fetal membranes were intact, but FHR was absent and repeat obstetric ultrasound confirmed fetal demise. Labor was induced with misoprostol and a female fetus was delivered 12 hours later via vaginal delivery. Significant changes have been noted in numerous investigations of placental tissue secondary to maternal COVID-19. However, clarification of the direct and indirect ways SARS-CoV-2 may affect the placenta are still under investigation (7, (9) (10) (11) . A recent study examining the physiologic interface between mother and fetus by Taglauer et al. found that SARS-CoV-2 spike glycoprotein (CoV2 SP) was present within the villious placenta of COVID-19-positive pregnant women with and without evidence of fetal transmission. Additionally, there was expression of viral entry proteins (ACE2 and TMPRSS2) located in an area of the placenta that is an important interface between mother and fetus (10) . These findings provide further insight into the potential underlying pathogenic mechanisms SARS-CoV-2 may utilize in pregnant women. Strengths of this report include laboratory-confirmed infection using reliable testing methods and the completion of fetal autopsy with family consent by an experienced pediatric pathologist. A limitation of this study is the single case. Additionally, fetal and placental SARS-CoV-2 RT-PCR testing was not performed. During hospitalization, requests for these tests by the J o u r n a l P r e -p r o o f Journal Pre-proof patient were denied, reportedly due to local health department restrictions in place at the time. This case and current evidence suggest that the adverse outcomes seen with other coronaviruses, including growth restriction, preterm delivery, miscarriage, and stillbirth are potential outcomes of COVID-19 infection during pregnancy (3, 7, 8) . As such, pregnant women with this novel coronavirus should be monitored closely and providers should act cautiously as they await further data and results of larger studies. Over 50,000 cases of confirmed SARS-CoV-2 infection and 60 deaths have been reported in pregnant women in the US since January 2020 (12) . These values likely underestimate the reach of SARS-CoV-2 in pregnancy due to poor data collection and limited testing capacity early in the pandemic. Additionally, significant rates of asymptomatic women with SARS-CoV-2 infeciton have been discovered upon obstetric hospitalization (4) (5) . Increased collection of COVID-19 data specific to pregnant women and their fetuses and infants is needed to increase knowledge and support research efforts, and can be utilized to create guidelines for clinical practice that will prevent potential negative outcomes and loss of life. 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