key: cord-0941099-5eg4can0
authors: Garin, Daniel; Cuillerier, Benoît; Dauendorffer, Jean-Noël; Crance, Jean-Marc; Lina, Bruno; Lozniewski, Alain; Da Conceição, Emmanuel; Jaulhac, Benoît; DeBriel, Dominique André
title: Diagnostic moléculaire en pathologie infectieuse: intérêt d'un diagnostic multiplex dans les pneumopathies atypiques
date: 1999-09-30
journal: Revue Française des Laboratoires
DOI: 10.1016/s0338-9898(99)80484-3
sha: a7e94e99d98a505c4432b1193a2ff152a9d916fd
doc_id: 941099
cord_uid: 5eg4can0

Résumé Parmi les pathogènes responsables de pneumopathies atypiques, Mycoplasma pneumoniae, Chlamydia pneumoniae, et Legionella pneumophila sont trois bactéries fréquentes pour lesquelles un diagnostic étiologique rapide est difficile à obtenir. L'amplification génique in vitro offre une possibilité de rendu de résultats dans la journée, mais souvent au détriment d'un temps de réalisation important du fait du manque d'automatisation. Cet inconvénient est réduit en cas d'utilisation de techniques d'amplification génique multiplex, dont un exemple de réalisation est présenté dans cet article. Abstract Among frequent potential respiratory pathogens, Mycoplasma pneumoniæ, Chlamydia pneumoniæ, and Legionella pneumophila are difficult to isolate with usual laboratory techniques (culture, serologic tests). Adapted molecular biology tools are able to amplify a specific genomic target and allow the diagnosis the same day, but with a very time consuming process. The proposed technique allows the diagnosis of the three bacteriaes in a few hours time using a multiplex single-tube polymerase chain reaction.

Un exemple de gel de la multiplex, ainsi que quelques exemples d'in- L'intBr6t que reprbsente le diagnostic des u pneumopathies atypiques * d'origine bactbrienne devrait entrainer rapidement la commercialisation de kits sp&cifiques.

Detection of M. pneumoniae and M. genifaliom in clinical samples by polymerase chain reaction

Detection of human M. pneumoniae by using the polymerase chain reaction

Detection of C. pneomoniae by polymerase chain reaction

Clinical overview of typical M. pneumoniae infections

Confirmed previous infection with C. pneumoniae (TWAR) and its presence in early coronary atherosclerosis

Comparaison of PCR, culture, and serological tests for diagnoses of M. pneumoniae respiratory tract infection in children

DNA sequence of MIP, a L. pneumophila gene associated with macrophage infectivity

Identification of C. pneumoniae by DNA amplification of the 16s rRNA gene

A new C. psittaci strain, TWAR, isolated in acute respiratory tract infections

Community and hospital acquired pneumonia associated with Chlamydia TWAR infection demonstrated serologically

pneumoniae (TWAR) infections in children

Rapid identiftiion of nine micro-organisms causing acute respiratory tract infections by single-tube multiplex reverse transcription-PCR: feasibility study

Use of multiplex PCR for simultaneous detection of four bacterial species in middle ear effusions

Prevalence of asymptomatic nasopharyngeal carriage of C. pneumortiae in subjectively healthy adults: assessment by polymerase chain reaction, enzyme immunoassay and culture

Serological diagnosis of M. pneumoniae infections : a critical review of current procedures

Revue Franc&e des Labor&ire& septembre 1999

Les auteurs remercient Nathalie Dorchain,