key: cord-0931956-kc6u0tts
authors: Juskewitch, Justin E.; Senefeld, Jonathon W.; Johnson, Patrick W.; Mills, John R.; Joyner, Michael J.; Gandhi, Manish J.
title: HLA Antibody Rates Are Not Increased In A Regional Group Of Male COVID-19 Convalescent Plasma Donors
date: 2021-08-20
journal: Mayo Clin Proc
DOI: 10.1016/j.mayocp.2021.08.004
sha: dc891639844de7068342440713ed5d8a9994450e
doc_id: 931956
cord_uid: kc6u0tts

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Justin E. Juskewitch Center, and South Texas Blood with sufficient residual serum and de-identified demographic data available were eligible for this cross-sectional study. All CCP donors provided permission to use de-identified donor information and serum samples for research. Given the de-identified nature of this study, clinical information regarding COVID-19 infection and past pregnancy, transfusion, or transplantation history was not available. Each male CCP serum sample was identified using the associated CCP unit's ISBT-128 donation identification number. This study was approved by the local institutional review board.

All CCP serum samples were screened for HLA-Abs using the same LABScreen Mixed Class I & II assays (One Lambda, West Hills, California) and performed by the same ASHI-J o u r n a l P r e -p r o o f

tissue typing laboratory that provides blood donor HLA-Ab screening. 1 Positive screening cut-off ratios for these HLA-Ab screening assays (class I ratio>30; class II ratio>18) had been previously established by the laboratory using a +5 standard deviation mark in a population of male never-transfused blood donors following a published methodology

The median donor age at the time of CCP collection was 48.8 years (range 16.0-85.5 years). Only two of the 526 male CCP donors screened positive for HLA-Abs (0.3%) within the expected <1% screening positivity rate. These two male CCP donors screened positive only for class I HLA-Abs (class I ratios of 33.74 and 60.91), were collected at different blood donation organizations, and were older at CCP donation (68.8 and 73.3 years) than the majority of male CCP donors

This larger cross-sectional study does not support an association between increased HLA-Ab screening positivity rates and recent COVID-19 infection amongst male CCP donors. These discepent findings between these two male CCP groups may be due to time differences since

Elevated Rate of HLA Antibodies in Male COVID-19 Convalescent Plasma Donors: A Risk Factor for Transfusion-Related Acute Lung Injury

Diagnostic and pathogenetic considerations in transfusionrelated acute lung injury

Safety of Convalescent Plasma Transfusion

Safety Update: COVID-19 Convalescent Plasma in 20,000 Hospitalized Patients

Establishing assay cutoffs for HLA antibody screening of apheresis donors

CRedIT Statement

Juskewitch -Conceptualization, Methodology, Formal Analysis, Writing -Original Draft

Senefeld -Methodology, Investigation, Resources, Writing -Review & Editing Patrick W. Johnson -Data Curation, Writing -Review & Editing, Visualization

We would like to thank the faculty and staff of South Texas Blood & Tissue Center, LifeSouth Community Blood Centers, Inc., Shepeard Community Blood Center, Kentucky Blood Center, and Mississippi Blood Services for their work and support in this study. We would also like to thank all of the CCP donors who volunteered throughout the COVID-19 pandemic to provide CCP products as well as research samples and deidentified donor data for ongoing CCP research. This research was supported by institutional funding for the Expanded Access Program and COVID-19-related research projects.