key: cord-0927680-05yt6nkb
authors: Granata, Vincenza; Fusco, Roberta; Setola, Sergio Venanzio; Galdiero, Roberta; Picone, Carmine; Izzo, Francesco; D’Aniello, Roberta; Miele, Vittorio; Grassi, Roberta; Grassi, Roberto; Petrillo, Antonella
title: Lymphadenopathy after BNT162b2 Covid-19 Vaccine: Preliminary Ultrasound Findings
date: 2021-03-11
journal: Biology (Basel)
DOI: 10.3390/biology10030214
sha: b61b42822f4b486f75a3f3a6317b7ee14a40e45e
doc_id: 927680
cord_uid: 05yt6nkb

SIMPLE SUMMARY: Eccentric cortical thickening with wide echogenic hilum and oval shape, asymmetric eccentric cortical thickening with wide echogenic hilum and oval shape, concentric cortical thickening with reduction in the width of the echogenic hilum and oval shape, and huge reduction and displacement of the echogenic hilum and round shape are the features that we found in post BNT162b2 Covid-19 Vaccine lymphadenopathies. ABSTRACT: During a spontaneous and autonomous study, we assessed the ultrasound finding of lymphadenopathy after BNT162b2 Pfizer vaccine. We enrolled 18 patients with 58 lymphadenopathies: in 10 patients, they were in the laterocervical side, while in 8 patients in the axillar site. The largest diameter was 16 mm with a range from 7 to 16 mm (median value = 10 mm). In the same patient, we found different ultrasound nodal findings. A total of 25 nodes showed eccentric cortical thickening with wide echogenic hilum and oval shape. In total, 19 nodes showed asymmetric eccentric cortical thickening with wide echogenic hilum and oval shape. Overall, 10 nodes showed concentric cortical thickening with reduction in the width of the echogenic hilum and oval shape. A total of four nodes showed huge reduction and displacement of the echogenic hilum and round or oval shape. No anomaly was found at the Doppler echocolor study. In conclusion, eccentric cortical thickening with wide echogenic hilum and oval shape, asymmetric eccentric cortical thickening with wide echogenic hilum and oval shape, concentric cortical thickening with reduction in the width of the echogenic hilum and oval shape, and a huge reduction and displacement of the echogenic hilum and round shape are the features that we found in post BNT162b2 Covid-19 Vaccine lymphadenopathies.

In December 2019, health authorities in Wuhan, China, identified a cluster of acute respiratory disease of unknown etiology [1] . Subsequently the researchers identified a new viral agent, SARS-CoV-2, as responsible for the heart of an international outbreak centered in Hubei. On 30 January 2020, World Health Organization (WHO) confirmed the COVID-19 epidemic as a public health emergency and on 11 March 2020 demarcated the rapid spread of infection as a pandemic in the world [1, 2] . Globally, at the time of writing (17 January 2021), there have been 93,194,922 confirmed cases of COVID-19, including 2,014,729 deaths, reported to WHO. In Italy, there have been 2,368,733 confirmed cases of COVID-19 with 81,800 deaths. Up to today, effective treatment has not yet been developed so that mechanical respiratory support is the only therapy in critically ill patients [3] [4] [5] . In this scenario, it was necessary to develop a vaccine as soon as possible, to prevent coronavirus disease 2019 and to protect persons who are at high risk for complications. At the time of writing in Italy have been approved two vaccines: the mRNA-1273 vaccine Moderna [6] and BNT162b2 Pfizer drug [7] . In 27 December, health authorities in Italy have authorized the administration of the BNT162b2 Pfizer vaccine in healthcare personnel, defining this day as Vaccine (V)-day.

During the trial clinic on BNT162b2, vaccine data on local and systemic reactions and use of medication were collected with electronic diaries from participants in the reactogenicity subset (8183 participants) for 7 days after each vaccination [7] . Adverse event analyses are provided for all enrolled participants, with variable follow-up time after dose. More BNT162b2 recipients than placebo recipients reported any adverse event (27 and 12%, respectively) or a related adverse event (21 and 5%). A total of 64 vaccine recipients (0.3%) and 6 placebo recipients (<0.1%) reported lymphadenopathy. We assessed ultrasound (US) findings in 18 consecutive patients from healthcare personnel that reported lymphadenopathy after BNT162b2 Pfizer drug.

This is a spontaneous and autonomous study, without the authorization of the ethics committee, for which patients have allowed data processing in accordance with National Privacy Regulations [8]. This observational study included the period from 27 December 2020 to 16 January 2021, the date of the last administration of the vaccine first dose among the health personnel in Campania. In this time range, 18 consecutive patients from healthcare personnel who received the first dose of BNT162b2 Pfizer vaccine were enrolled: 10 for palpable mass appeared after the vaccine, 8 sent to the ultrasound study by the pharmacovigilance physician.

In Table 1 we reported the patients study group characteristics. 

Nodes ultrasound exams were performed by dedicated radiologists, using RS85 Samsung System (Samsung Healthcare GmbH, Schwalbach, Germany) in combination with a linear 5 to 12-MHz array transducer.

A total of four in-site expert radiologists in interpretation of nodal images, recorded the data in consensus. Presence, side, size, shape, echogenicity, cortex feature, margin, and hilum of the lesions were categorized. We also assessed color doppler features.

We assessed 18 patients with 58 lymphadenopathy after BNT162b2 Covid-19 Vaccine. In 10 patients (55.5%) they were in laterocervical side while 8 (44.5%) in the axillar site. The largest diameter was 16 mm with a range from 7 to 16 mm (median value = 10 mm). In the same patient we found different ultrasound nodal findings.

No anomaly was found on the Doppler echo-color study. A total of 25 (43.1%) nodes showed eccentric cortical thickening with wide echogenic hilum and oval shape (Figures 1 and 2) . Overall, 19 nodes (32.8%) showed asymmetric eccentric cortical thickening with wide echogenic hilum and oval shape (Figure 3) . 

Recent results have revealed the efficiency of some imaging methods, including chest radiographs and chest computed tomography scans, in the management of COVID-19 disease [8 -28] . Instead, at the best of our knowledge, this is the first paper describing the appearance of nodes after BNT162b2 Covid-19 vaccine. Although the onset of lymphadenopathy after vaccine is known, Polack et al reported a frequency of only 0.3% [29] . However, we think that the date may be underestimated, in consideration of our sample size, although it is a representative population of a small community. Moreover, although in 10 patients the lymph nodes were painful, in four patients the data were an occasional finding diagnosed during a US examination performed for another reason. A total of 10 nodes (17.2%) showed concentric cortical thickening with reduction in the width of the echogenic hilum and oval shape (Figure 1b) .

In total, four nodes (6.9%) showed huge reduction and displacement of the echogenic hilum and round or oval shape (Figure 4 ). 

Recent results have revealed the efficiency of some imaging methods, including chest radiographs and chest computed tomography scans, in the management of COVID-19 disease [8 -28] . Instead, at the best of our knowledge, this is the first paper describing the appearance of nodes after BNT162b2 Covid-19 vaccine. Although the onset of lymphadenopathy after vaccine is known, Polack et al reported a frequency of only 0.3% [29] . However, we think that the date may be underestimated, in consideration of our sample size, although it is a representative population of a small community. Moreover, although in 10 patients the lymph nodes were painful, in four patients the data were an occasional finding diagnosed during a US examination performed for another reason. 

Recent results have revealed the efficiency of some imaging methods, including chest radiographs and chest computed tomography scans, in the management of COVID-19 disease . Instead, at the best of our knowledge, this is the first paper describing the appearance of nodes after BNT162b2 Covid-19 vaccine. Although the onset of lymphadenopathy after vaccine is known, Polack et al reported a frequency of only 0.3% [7] .

However, we think that the date may be underestimated, in consideration of our sample size, although it is a representative population of a small community. Moreover, although in 10 patients the lymph nodes were painful, in four patients the data were an occasional finding diagnosed during a US examination performed for another reason.

US is commonly regarded as the imaging modality of choice in the assessment of palpable soft-tissue abnormalities. According to the appropriateness criteria of the American College of Radiology, US is "usually appropriate" to evaluate superficial or palpable soft-tissue masses, while magnetic resonance imaging (MRI) is "usually appropriate" in case of non-diagnostic initial US evaluation [31] , while CT is appropriate for assessment of systemic diseases [32] .

Lymph node cortical thickness and uniformity are the most important criteria for distinguishing between normal and abnormal nodes. Normal lymph nodes have a reniform shape, a uniformly hypoechoic cortex with a maximal thickness of 3 mm, smooth margins, and a central fatty hilum [33] . Findings of cortical thickness in excess of 3 mm, eccentric thickening, irregular margins, and encroachment on or displacement of the fatty hilum are suggestive of a pathologic process [33] .

We found different US findings in the same patient; 43.1% of lymph nodes showed eccentric cortical thickening with wide echogenic hilum and oval, 32.8% of lymph nodes showed asymmetric eccentric cortical thickening with wide echogenic hilum and oval shape. A total of 17.2% of lymph nodes showed concentric cortical thickening with reduction in the width of the echogenic hilum and oval shape and 6.9% showed huge reduction and displacement of the echogenic hilum and round or oval shape.

We believe it is important to know and recognize these structural alterations, since as soon as the vaccine will be also available to non-healthcare personnel, it is important for a radiologist to be able to identify post-vaccine lymphadenopathy compared to lymphadenopathy for another cause, especially in cancer patients.

There are multiple limitations of this work. First of all, the small and spontaneous sample, consequently we did not know the real incidence of lymphadenopathy. Second, not having performed an elastosonographic and echocontrastographic examination; finally, the absence of a pathological correlation.

Future objective is an evaluation of the entire population of our institution undergoing vaccination for a correct estimate of the incidence and a more complex ultrasound analysis.

Eccentric cortical thickening with wide echogenic hilum and oval shape, asymmetric eccentric cortical thickening with wide echogenic hilum and oval shape, concentric cortical thickening with reduction in the width of the echogenic hilum and oval shape, and huge reduction and displacement of the echogenic hilum and round shape are the features that we found in post BNT162b2 Covid-19 Vaccine lymphadenopathies. Institutional Review Board Statement: "National Cancer Institute of Naples Pascale Foundation" Institutional Review Institute (IRB) approved the study and renounced the written informed consent for this retrospective study, considering the ongoing epidemic emergency, which assessed the unidentified data and did not involve risks potential for patients.

Informed Consent Statement: Consent for publication was received.

The authors are grateful to Alessandra Trocino, librarian at the National Cancer Institute of Naples, Italy. Moreover, they are grateful to Assunta Zazzaro for the collaboration.

The authors declare no conflict of interest.

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