key: cord-0925731-velb4fxq authors: Fancello, Virginia; Hatzopoulos, Stavros; Corazzi, Virginia; Bianchini, Chiara; Skarżyńska, Magdalena B; Pelucchi, Stefano; Skarżyński, Piotr Henryk; Ciorba, Andrea title: SARS-CoV-2 (COVID-19) and audio-vestibular disorders date: 2021-06-18 journal: Int J Immunopathol Pharmacol DOI: 10.1177/20587384211027373 sha: d5e542f6bc0d1faa9ca07823c8ae21e9a2c105a1 doc_id: 925731 cord_uid: velb4fxq To describe the audio-vestibular disorders related to the newly SARS-CoV-2 infection, including the possible ototoxicity side-effects related to the use of drugs included in the SARS-CoV-2 treatment protocols. A systematic review was performed according to the PRISMA protocol. The Medline and Embase databases were searched from March 1, 2020 to April 9, 2021. Initially the search yielded 400 manuscripts, which were reduced to 15, upon the application of inclusion criteria. Sensorineural hearing loss (SNHL) is the most frequent audio-vestibular symptom described, occurring alone or in association with tinnitus and vertigo. The etiopathogenesis of the inner ear disorders related to COVID-19 infection is still poorly understood. The number of reports of COVID-19 infections associated to audio-vestibular disorders is increasing; even if the quality of the studies available is often insufficient, audio-vestibular disorders should be considered as possible manifestations to be included among the symptoms of this infection. Since the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection, several authors have tried to outline a comprehensive clinical description of the disease, including its possible audiological manifestations. 1 The relevance of olfactory and gustatory dysfunction, alone or in association with other symptoms, supports the hypothesis of the SARS-CoV-2 neurotropic and neuroinvasive properties, as observed in other coronaviridae infections. 2, 3 Also, neurological manifestations have been reported as possible complications of up to 30% of patients affected by COVID-19. 4 Among the nervous structures, the inner ear can be a possible target of the virus, and the induced damage can manifest as sensorineural hearing loss (SNHL), tinnitus and/or vertigo. However, the scientific literature focused on this topic is still in its early stages. Aim of this systematic review is to describe the audio-vestibular disorders related to the newly SARS-CoV-2 infection, including the possible ototoxicity side-effects related to the use of drugs administrated in the SARS-CoV-2 treatment protocols. A systematic review was performed following PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) protocol, of Englishlanguage studies focused on the audio-vestibular manifestations (including SNHL, tinnitus and vertigo) of the newly COVID-19 infection via the online database Medline and Embase. The structure of the search strategy was adapted to suit both databases. The keywords and Mesh terms 'COVID-19', 'Hearing Loss', 'Vertigo', 'Tinnitus', 'inner ear', 'hearing impairment' and 'ototoxicity' were used to select the studies of interest. Case reports and case series were included. The last literature search was done on 9th April 2021. The search keywords yielded a total number of 400 papers. These were filtered by the following inclusion/exclusion criteria: Inclusion: -Direct temporal correlation between the new-onset of SNHL, vertigo and/or tinnitus and COVID-19 infection. -Established SARS-CoV-2 infection by Polymerase Chain Reaction (PCR), detection of specific antibodies by serology testing or chest CT scan suggestive of COVID-19 or reported admission to the hospital for COVID-19. Exclusion: -Not established SARS-CoV-2 infection. -Studies containing duplicated data from other published work. -Studies reporting cases of conductive hearing loss. -No direct temporal correlation between audiovestibular symptoms and COVID-19. -Studies published in languages other than English. Of the 400 papers initially selected, only 15 passed the selection criteria. The PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) diagram of the selection process, is illustrated in Figure 1 . The database search identified 15 case reports or case series (level of evidence 4) for a total of 20 patients with established SARS-CoV-2 infection. [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] All patients reported a new-onset of audiovestibular symptoms. The mean patient age was 42.4 ± 35.1 years, while the male to female ratio was 7:13. The documented time-interval, between the SARS-CoV-2 infection and the onset of audio-vestibular symptoms, was maximally a 6-week period. 17 Major findings such as: first author, country of the study, patient's characteristics, type of audiovestibular manifestations, investigations, treatments and outcomes are summarized in Table 1 . Sensorineural hearing loss (SNHL) was the most frequent audio-vestibular symptom described in 15 out of the 20 patients: it was described as isolated SNHL in five cases, associated to tinnitus in seven cases and to tinnitus/vertigo in three subjects (see Table 1 ). The first postulated correlation between SNHL and COVID-19 dates back to April 2020, but the association appeared coincidental, and the patient details were not provided (Sriwijitalai and Wiwanitkit). 5 Since then, various anecdotal cases of unilateral or bilateral SNHL, alone or in association to tinnitus or vertigo, have been described. In a few reports, hearing loss has been only supposed and not documented, while some authors theorized a direct correlation to COVID-19 based on a temporal association, a plausible pathophysiology and the absence of relevant medical history or other risk factors rather than the viral infection. 13, 20 Overall, the available studies evidenced that the audiological examination (i.e. audiometric testing) was challenging, due to the sanitation requirements all the equipment and the clinical environment after each evaluation. Pure tone audiometry was performed in 10 subjects with a new onset of SNHL. Two papers (Degen et al., 8 Chern et al. 16 ) report data from a binaural SNHL. In both cases, the cerebral MRI found evidence of inner ear damage, a bilateral intra-labyrinthine haemorrhage and a bilateral cochlear inflammation, respectively. One patient, not responsive to steroid therapy, underwent a cochlear implant under local anaesthesia, due to the presence of cochlear fibrosis evidenced by MRI (Degen et al. 8 ). Another issue, since the early stages of the pandemic, is the treatment of the audio-vestibular disorders, and in particular the treatment of SNHL. Even if steroids are usually administrated in subjects affected by SNHL of a newly onset, their use in the early stages of viral infections should be considered carefully, because of the risk of causing a delayed viral clearance and a consequent deterioration of the general health status due to the infection. 21 Therefore, aiming the avoidance of adverse systemic effects, the intratympanic injection is the ideal route of administration, due to the minimal systemic but higher perilymphatic absorption. 22 Descriptions of vestibular deficit associated to COVID-19 infection have been documented, but a comprehensive vestibular examination is rarely available. Malayala et al. 6 reported on six cases of vestibular deficit from different parts of the world, but coexistent SARS-CoV-2 infection was confirmed in four over six subjects. Unfortunately, the evidence of nystagmus in these series was described in only two cases, challenging any hypothesis about the correlation of specific vestibular disorder (i.e. neuritis, benign paroxysmal positional vertigo or Ménière disease) and the viral infection. A complete vestibular evaluation is difficult to achieve in COVID-19 patients, as protective equipment and careful disinfection of all surfaces is required. In addition, the chance of triggering vomiting during the examination increases the risk of contagion. As a result, only one report described a comprehensive vestibular assessment, including a vHIT (video Head Impulse Test) evaluation (Mat et al. 18 ) . A newly onset vertigo should be always investigated carefully, especially when a peripheral vestibular deficit is not certain. Differential diagnosis from acute cerebrovascular events is mandatory; in particular, it is necessary to exclude acute thrombosis of the intracranial arteries (vertebral artery and PICA (posterior inferior cerebellar artery)) as already reported in a patient affected by COVID-19, and manifesting vertigo and nystagmus. 23 Tinnitus was described as a concomitant symptom in 11 over 20 subjects, while isolated presentations were not reported. The specific features of tinnitus were not reported in all studies; only one evaluated the matching frequency and intensity, revealing the tinnitus perception at 10 dB of 4 kHz; the impact on life quality and definitive resolution were not routinely registered. 15 A survey on 185 patients with positive PCR nasopharyngeal swabs reported that tinnitus started after COVID-19 diagnosis in 23.2% of cases. 24 Emotional factors, anxiety, poor sleep quality experienced during the course of the disease can play a relevant role in developing or enhancing this symptom, which appears to be more bothersome for those quarantined. 25 Patients affected by COVID-19 often receive medication with potential ototoxic effects. 26 The administration of hydroxychloroquine especially is a discussion topic, because of its wide offlabel use in the early times of the pandemic. The subset of neural, retinal and audio-vestibular adverse manifestations caused by quinine and derivatives, including hydroxychloroquine, are well known since the 18th century with the name of 'cinchonism'. 27, 28 Nowadays the major Health Surveillance Agencies, because of the lack of evidence about potential benefit and the well-known side effects, restricted its prescription as a part of approved clinical trials under close monitoring. 29 Azithromycin, a macrolide antibiotic with known anti-inflammatory properties, was proposed in multiple treatment protocols for COVID-19. In vitro and in vivo ototoxic effects of this class of antibiotics, including azithromycin, have been reported. 30 A recent meta-analysis and literature review underlined that the frequency of SNHL due to exposure to macrolide antibiotics is higher compared to that of other antibiotics or placebo, even at standard oral doses. 31, 32 The drug combination lopinavir-ritonavir (antiviral protease inhibitor) is listed among current treatments against SARS-CoV-2. Few reports described anecdotal cases of hearing loss associated with their use, postulating possible mitochondrial toxicity as a potential etiologic mechanism. 33 Another antiviral agent, ribavirin, is known to cause severe SNHL if prescribed alongside interferon, even if the data about ototoxic effects of ribavirin alone are scarce. 34 Specific references on the history of exposure to ototoxic drugs were reported in 4 over 20 patients and these cases are included in this review. 7, 10, 14, 24 All patients received hydroxychloroquine alone or in association to other established potential ototoxic drugs, such as azithromycin or furosemide; a clear correlation between their symptoms and the adverse effects of these medications was not established. 38 The uncontrolled recent growth of literature, among the SARS-CoV2 infection, raised the problem of how to establish a relationship between the virus and the inner ear damage. 35 An accurate anamnesis, including previous audiological events, is crucial to guide the clinician in the identification of the etiopathogenesis. Previous history of noise exposure, head trauma, autoimmune diseases, exposure to ototoxic drugs, Ménière disease, anatomical alterations of the ear and other predisposing factors should be always ruled out before postulating a link to SARS-CoV-2. In addition, Magnetic Resonance Imaging (MRI) is required to exclude retro-cochlear pathology. The damage of the audio-vestibular system during a SARS-CoV2 infection could be related to a direct impairment of inner ear structures or to a virus-mediated immune response. Blood vessels, lymphatics and nerves and in some cases the meninges (as proposed by Degen et al. 8 ), have been proposed as entry routes for the virus. 8 The hypothesis of a possible direct damage to the cochlear hair cell has been supported by studies reporting a reduced amplitude of transient otoacoustic emissions (TEOAEs) in infected adults and even in newborn patients exposed to SARS-CoV-2 intrauterinely. 36, 37 Some Authors have advocated a potential inflammatory involvement of inner ear vessels or of the stria vascularis (eventually causing vasculitis or endothelitis), since its highly vulnerability to hypoxic damage. 38 In fact, brain microhaemorrhages have been reported to be another peculiar sign of COVID-19 infection. 39 In effect, bilateral intralabyrinthine haemorrhage has been documented in a case of bilateral SNHL and vertigo in a patient with a history of SARS-CoV-2 and in a patient with unilateral SNHL and diffuse cerebral microhaemorrhages (Lamounier et al. 12 and Chern et al. 16 ). A cerebral MRI was performed in 10 of the 20 patients with audio-vestibular disorders. The neuroimaging revealed a clear inflammatory process of the inner ear in two of them, in one case bilateral, supporting the hypothesis of a direct cochlear and labyrinth damage (Degen et al. 8 and Perret et al., 19 ). Other proposed etiopathogenetic mechanisms have been related to (i) the presence of a persistent inflammatory status with the production of proinflammatory cytokines that may hamper the inner ear functions 36 ; and (ii) to the concomitant onset of an autoimmune response, such as granulomatosis with polyangiitis. 40 Drawbacks. This review considered among other data individual reports and case series (level of evidence 4), therefore it is susceptible to information bias, which might represent a limitation of the study. Furthermore, in some of the reported cases the absence of audiological examination prior to the onset of the acute hearing loss, is another possible drawback since it is not possible to compare pre and post hearing threshold levels. This aspect has been discussed by other Authors 41, 42, 43, 44 ; in some of the reported cases, it is not clear if the symptoms represent the worsening of a pre-existing disease triggered by SARS-CoV-2, or a clinical manifestation entirely related to the virus, or a coincidental event. Still little is known among the possible inner ear involvement of the newly COVID-19 infection and in particular among the possible pathophysiology of the associated inner ear disorders. At present, there is only a very limited amount of literature focusing on this topic and mainly case reports; however, SNHL, tinnitus and vertigo should be considered as possible manifestations to be included between the symptoms of this infection. Furthermore, due to the potential ototoxicity related to the use of some medications in the treatment of the COVID-19 infection, it is necessary to strictly monitor the use of these drugs because induced hearing loss, vertigo and/or tinnitus can be irreversible. 45, 46 Finally, in our opinion, due to several factors such as: (i) the often-precarious conditions of patients affected, and (ii) the highly infectivity and the rapid spread of the virus, Otoacoustic Emissions (OAEs) protocols could provide information about the cochlear function in a simple, fast, non-invasive and feasible way. 47 Since no subject-cooperation is required, OAEs may represent a first line 'audiological' tool, for assessing patients affected by COVID-19 and complaining auditory symptoms, directly at home or in COVID-19 wards. The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. 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