key: cord-0924518-84s384n3
authors: Yuan, Yue; Jacobs, Caron A.; Llorente Garcia, Isabel; Pereira, Pedro M.; Lawrence, Scott P.; Laine, Romain F.; Marsh, Mark; Henriques, Ricardo
title: Single-Molecule Super-Resolution Imaging of T-Cell Plasma Membrane CD4 Redistribution upon HIV-1 Binding
date: 2021-01-19
journal: Viruses
DOI: 10.3390/v13010142
sha: 6515b9d3132008b651bc85fc3043d25d95e178e5
doc_id: 924518
cord_uid: 84s384n3

The first step of cellular entry for the human immunodeficiency virus type-1 (HIV-1) occurs through the binding of its envelope protein (Env) with the plasma membrane receptor CD4 and co-receptor CCR5 or CXCR4 on susceptible cells, primarily CD4(+) T cells and macrophages. Although there is considerable knowledge of the molecular interactions between Env and host cell receptors that lead to successful fusion, the precise way in which HIV-1 receptors redistribute to sites of virus binding at the nanoscale remains unknown. Here, we quantitatively examine changes in the nanoscale organisation of CD4 on the surface of CD4(+) T cells following HIV-1 binding. Using single-molecule super-resolution imaging, we show that CD4 molecules are distributed mostly as either individual molecules or small clusters of up to 4 molecules. Following virus binding, we observe a local 3-to-10-fold increase in cluster diameter and molecule number for virus-associated CD4 clusters. Moreover, a similar but smaller magnitude reorganisation of CD4 was also observed with recombinant gp120. For one of the first times, our results quantify the nanoscale CD4 reorganisation triggered by HIV-1 on host CD4(+) T cells. Our quantitative approach provides a robust methodology for characterising the nanoscale organisation of plasma membrane receptors in general with the potential to link spatial organisation to function.

Cell surface receptor binding is a key step in cell infection by viruses, initiating processes that allow viral particles to cross the plasma membrane and deliver their genetic material to the host cell [1] . To infect CD4 + T cells, and other immune cells such as macrophages, the human immunodeficiency virus type-1 (hereafter referred to as HIV) requires binding to the surface glycoprotein CD4, and either CCR5 or CXCR4 (depending on strain tropism) as co-receptors. Biochemical and structural studies have led to a well-developed biomechanical model of the conformational changes in the viral envelope in the absence of HIV). We further explored the reorganisation of CD4 clusters with statistical modelling: Cluster-size specific Poisson statistics were used to model the distributions of CD4 molecules. We found that the receptor-number distribution in HIV-bound cells differs from that predicted by a random distribution of receptors. Together, these approaches provide a novel view of the initial events of HIV binding and entry and indicate a link between a functional role and the spatial organisation of cell-surface receptors. The imaging and analytical pipeline we set out here can be used to explore the nanoscale distribution and function of other cell-surface receptors, opening the way to new insights into the molecular events underlying signal transduction.

SupT1 ([VB] ATCC ® CRL-1942™) is a CD4 + /CCR5 − cell line derived from a T-cell lymphoblastic lymphoma. SupT1-R5 is a stable CCR5 + derivative of SupT1 provided by James A. Hoxie (University of Pennsylvania). Suspension cultures of SupT1 and SupT1-R5 were maintained in phenol red-free RPMI 1640 medium (Life Technologies, 32404-014) supplemented with 10% foetal bovine serum (FBS; Sigma-Aldrich, Dorset, UK, F9665), GlutaMAX Supplement (Life Technologies, Paisley, UK, 35050-038), 50 U/mL penicillin, and 50 µg/mL streptomycin at a density of 1 × 10 5 -1 × 10 6 cells/mL at 37 • C with 5% CO 2 .

Human embryonic kidney (HEK) 293T cells (ATCC ® CRL-3216™) were cultured in Dulbecco's Modified Eagle Medium (DMEM; Life Technologies, Paisley, UK, 31053-028) supplemented with 10% FBS, GlutaMAX Supplement, 50 U/mL penicillin, and 50 µg/mL streptomycin at 37 • C with 5% CO 2 .

HeLa-TZM-bl cells were cultured in DMEM, supplemented with 10% FBS and 1% GlutaMAX Supplement at 37 • C with 5% CO 2 .

The monoclonal antibody OKT4 (anti-CD4) [35] was conjugated with Alexa Fluor fluorophores using NHS-ester chemistry Lightning Link Kits (Innova Biosciences, Cambridge, UK). Briefly, 10 µg IgG (1 mg/mL in PBS) were mixed with 1 µL of LL-Modifier reagent, before the addition of 2-3 molar equivalents of NHS ester-functionalised Alexa Fluor 568 or Alexa Fluor 647 (from 10 mg/mL stocks in DMSO). The reaction mixture was incubated at room temperature (RT, 23 • C) for 3-4 h. Subsequently, 1 µL of LL-Quencher was added to terminate the reaction. Unreacted fluorophore was removed by diluting the reaction volume to 500 µL and centrifugation through 3 kDa MWCO Amicon Ultra centrifugal filter columns at 14,000 relative centrifugal force (RCF) for 15 min. This washing step was repeated three times. Protein concentration and protein:dye ratio were determined by spectrophotometric measurement of absorbance at 280 nm and 280 nm vs. 647 nm or 568 nm, respectively. Antibody cell labelling specificity was confirmed by comparison of immunolabeling signals of CD4 + (Jurkat and SupT1) and CD4 − (HEK293T) cells by epifluorescence microscopy.

SupT1-R5 cells (1 × 10 4 cells per sample) were pelleted and resuspended in 20 µL cold RPMI-1640 with 0.4% FBS and 6 µg/mL OKT4-Alexa Fluor 647. Cells were incubated on ice for 60 min before washing, which was carried out by making up the cell volume to 10 mL in cold RPMI with 5% FBS and centrifuging the cells at 300 RCF for 6 min at 4 • C. The supernatants were carefully aspirated and the cells washed twice in 10 mL cold RPMI-1640 with 5% FBS, as described above. Cells were resuspended in 60 µL of cold RPMI-1640, with 5% FBS, and allowed to settle on Poly-L-lysine (PLL)-coated coverslips (100 µg/mL in ddH 2 O, Sigma-Aldrich, Dorset, UK, P8920) at 4 • C for 40 min. Treated samples were transferred to 37 • C RPMI-1640 with 5% FBS, with or without 2 mg/mL Phorbol-12-myristate-13-acetate (PMA) or 4-α-Phorbol-12-myristate-13-acetate (4α-PMA) and incubated for 15 min at 37 • C. Thereafter, cells were returned to cold RPMI-1640 for 1-5 min and fixed with cold 4% paraformaldehyde (PFA) for 10 min. Control samples were transferred directly to cold 4% PFA without warming. Samples in PFA were then warmed to 37 • C over 20 min, washed five times in PBS, and stored in PBS until mounting for imaging.

HEK293T cells were seeded at 2.25 × 10 6 cells per T75 culture flask to give <50% cell confluency the following day. 1.5 mL of OptiMEM was mixed with 15 µg of HIV JR-CSF proviral DNA and incubated at RT for 5 min, before gently mixing with 45 µL Fugene 6 equilibrated to RT. Subsequently, cell medium was replaced with 15 mL antibiotic-free DMEM containing 10% FBS, after which the transfection reaction mixture was added and the cells were incubated at 37 • C with 5% CO 2 . After 48 h, the culture medium was collected and centrifuged at 500 RCF for 10 min. The supernatant was transferred to Beckman ultracentrifuge tubes underlaid with a 5 mL cushion of sterile 20% sucrose in PBS. The tubes were topped up with complete media and the virus pelleted through the sucrose cushion by ultracentrifugation at 98,000 RCF for 2 h at 4 • C. The supernatant was carefully aspirated to preserve the viral pellet, which was then resuspended in DMEM, aliquoted, and stored at −80 • C in liquid nitrogen.

HeLa-TZM-bl cells were seeded at 1 × 10 3 cells per well in a 96 well microtiter plate. After 12-18 h, the cells were infected with HIV in a series of two-fold dilutions from 1 in 2 to 1 in 256 in a final volume of 400 µL. At 6 h post-infection (hpi), 8 µg Q4120 was added to each well to prevent syncytia formation. Cells were washed in PBS at 36 hpi and fixed in 4% PFA for 30 min at RT, followed by incubation in 0.1% PFA overnight at 4 • C. Cells were then washed three times with PBS at RT, quenched in 50 mM NH 4 Cl for 10 min, blocked and permeabilised with PBS supplemented with 1% FBS and 0.1% Triton for 15 min at RT, and finally washed with 1% FBS in PBS. Cells were incubated with rabbit antiserum to HIV p24/p55 Gag (ARP432; NIBSC Centre for AIDS Reagents, South Mimms, UK) at 1:500 in PBS containing 1% FBS for 1 h at RT, washed three times with PBS/1% FBS and incubated with Goat anti-rabbit IgG-Alexa Fluor 488 (H+L; Life Technologies, A-11008) and DAPI for 20-30 min. Cells were washed with PBS before imaging the plates using a Perkin Elmer Opera Phenix high-throughput plate reader with a 20× air objective. Images (9 per well) were analysed using Columbus Image Analysis software.

2.6. HIV Binding 1 × 10 4 SupT1 and SupT1-R5 were pre-incubated with OKT4-Alexa Fluor 647 (6 µg/mL) in a total volume of 20 µL for 15 min at RT. The cells were then cooled to 4 • C. Initially three different HIV multiplicities of infection (MOI) were tested to find a balance between having a sufficient number of bound particles per field of view (FOV) and a relevant number of viruses. MOIs of 15, 30 and 50 infectious units per cell were tested. We observed an average of 1, 23 and 42 HIV particles per cell, respectively. In order to maximise the experimental throughput while remaining within reasonable bounds of viral infection, we chose an MOI of 30 as the being optimal. After 1 h, free virus was removed by diluting the cells in 10 mL cold RPMI-1640 and centrifugation at 500 rpm for 10 min. For 0 min warm up samples, the cell pellets were resuspended in 300 µL cold serum-free RPMI-1640 and allowed to settle onto PLL-coated µ-Slide 8 Well dishes (Ibidi GmbH, Gräfelfing, Germany) for 40 min. For 1 min warm up samples, the cells were resuspended in 50 µL serum-free RPMI-1640 and transferred to a water bath at 37 • C. After 1 min, 10 mL cold serum-free RPMI-1640 were added to the sample tube, followed by centrifugation at 500 rpm for 10 min. The cell pellets were then resuspended in 300 µL serum-free RPMI-1640 and allowed to settle onto PLL-coated µ-Slide 8 Well dishes for 40 min. Settled cells were fixed directly by incubation with 4% PFA in PBS for 30 min at RT (control sample), or the cold media was replaced with pre-warmed media, and samples incubated at 37 • C for 1 min before fixing as described above. After fixation, samples were washed three times with PBS and permeabilised in 0.1% Tween-20 in PBS for 5 min, blocked for 20 min in PBS containing 4% BSA, and labelled with anti-HIV Gag antiserum as above. The cells were then washed three times in PBS and incubated with 1:500 Goat-anti-rabbit-Alexa Fluor 568 (Life Technologies, A-11036) for 1 h at RT, then washed five times in PBS (5 min for each wash), subjected to a second round of fixation with 4% PFA in PBS for 30 min at RT, washed five times in PBS and stored in PBS before mounting for analysis by microscopy.

SupT1 and SupT1-R5 cells (1 × 10 4 cells per sample) were pelleted and resuspended in 20 µL cold RPMI-1640 with 0.4% FBS and 6 µg/mL OKT4-Alexa Fluor 647, with or without 1 µg/mL HIV BaL gp120 (NIH AIDS Reagents Program). Cells were incubated on ice for 60 min before being diluted in 10 mL cold serum-free RPMI-1640 and centrifuging at 300 RCF for 6 min at 4 • C. The supernatants were carefully aspirated, and the cells resuspended and washed twice in 10 mL cold serum-free RPMI-1640. The cells were then resuspended in 60 µL cold serum-free RPMI-1640 and allowed to settle on PLL-coated coverslips (100 µg/mL in ddH 2 O, Sigma-Aldrich, Dorset, UK, P8920), on ice, for 40 min. Control samples were transferred directly to cold 4% PFA. Treated samples were transferred to 37 • C serum-free RPMI-1640 for 1 min, before returning to 1 mL cold serum-free RPMI-1640 for 5 min to rapidly cease cells' reaction to treatment. The cells were then fixed with cold 4% PFA for 10 min, before warming to 37 • C over 20 min. Samples were washed five times in PBS and stored in PBS until mounting for imaging.

Samples prepared on coverslips were mounted on parafilm-formed gaskets (as described in [36] ) with STORM buffer (150 mM Tris pH 8, 1% glycerol, 1% glucose, 10 mM NaCl, 1% beta-mercaptoethanol, 0.5 mg/mL glucose oxidase, and 40 µg/mL catalase) and sealed with clear nail varnish. For samples on Ibidi GmbH slides, the sample wells were filled with STORM buffer, and the lid sealed with High-Performance Black Masking Tape (Thorlabs, UK).

Imaging was carried out on a Zeiss Elyra PS.1, with an alpha Plan-Apochromat DIC M27 Elyra 100 × 1.46 Numerical Aperture (NA) oil objective, additional 1.6× optovar magnification, and Andor iXon 897 electron multiplication CCD (EMCCD) camera, yielding a pixel size of 100 nm. STORM datasets of 15,000 sequential frames were acquired in Total internal reflection fluorescence (TIRF) configuration, using 33 ms exposure time, with 642 nm or 561 nm excitation at maximum power output (approximately 3.98 kW/cm 2 and 3.71 kW/cm 2 on the sample, respectively). Fluorophore photoswitching was dynamically controlled using periodic 405 nm illumination at the intensity of approximately 0-0.0586 kW/cm 2 on sample laser power. EM camera gain of 300 was used. Microscope autofocus was used throughout all acquisitions.

SMLM imaging datasets were processed using the ThunderSTORM analysis plugin [37] . Initial particle localisation was performed using the local maximum method, followed by sub-pixel localisation using the integrated Gaussian model and fitting by Maximal Likelihood Estimation. Drift correction was performed post-localisation by cross-correlation (number of bins was 5.0). Reconstructed images were rendered using a normalised 20 nm Gaussian.

For cluster analysis, we used Clus-DoC [38] . 30 nm Epsilon and 1 Minimum point parameters were used throughout the study. We manually selected our regions of interests (ROIs) to include as much of the plasma membrane as possible while avoiding cell-edge effects. For each cluster, we obtained the area and corresponding mean diameter, the cluster density per ROI (number of clusters detected/ROI area [µm 2 ]) as well as the number of localisations for each cluster.

To validate the quantitative results from Clus-DoC [38] , we manually annotated clusters in Fiji [39] ; cross-sections of identified clusters were drawn individually. The intensity profile was plotted and fitted with a Gaussian distribution of standard deviation σ. The cluster diameter was estimated using the Full-Width Half Maximum (FWHM) as d = 2.35 σ.

Channel registration was performed using a chromatic aberration correction plugin developed by the Jalink lab (https://jalink-lab.github.io/). Fiducial bead-coated coverslips were imaged for each experiment under both 647 nm and 568 nm channels. Fiducial bead images were used as references for estimating the transformation between channels. This correction was directly measured and applied to localisation data.

To determine the colocalisation threshold, we used post-channel registration fiducial bead images. When the optimised threshold was set to 0.4, the majority of events co-localised (>99%) with a peak of the DoC distribution at 1, indicating high colocalisation. Aside from the DoC score, we also extracted information on the percentage of colocalisation between channels and the mean cluster diameter in the colocalised and non-colocalised areas.

We performed molecular counting as described [32] . To calibrate the grouping parameters, we performed the same STORM imaging procedure as described in 2.9 on isolated fluorophore-conjugated antibodies. The imaging dishes were incubated with 1 µg/mL dye-conjugated anti-CD4 antibody for 15 min. The dishes were washed once and then imaged under the same experimental conditions as for the main dataset using T cells. Images were reconstructed as described in 2.10. Localisations within 30 nm were merged as one localisation frame by frame to form a new coordinates map and temporally binned to extract calibration parameters for molecular counting. All localisation processing was performed using custom-written MATLAB (MathWorks) scripts (kindly provided by Christian Sieben). Alignment of localisations from individual molecules also allowed for estimation of the localization precision, as described in [32] .

We used a theoretical model based on cluster-size specific Poisson probability distributions to determine whether our results for CD4 molecule numbers in clusters are consistent with a random distribution of receptors on the cell surface.

We generated theoretical distributions of the number of molecules per cluster expected for a random distribution of receptors using an average CD4 surface density n = 200 molecules/µm 2 . This comes from considering a CD4 density of approximetaly 100,000 molecules/cell in SupT1-R5 cells [40, 41] and typical T-cell shapes with a radius of approximately 10 µm. Cells were approximated as a flat disk with total surface area 2 × πr 2 = 630 µm 2 .

The expected probability of observing a number of receptors k in a given area A of the cell surface is given by the discrete Poisson probability distribution, P(k; λ), where k ≥ 0 and λ = nA is the mean receptor count expected when counting receptors on a patch of area A (λ is also the mean of the distribution). This simple model assumes independence of receptor-counting events and, hence, no interactions between receptors, signalling, or active processes or receptor mobility are considered. Thus, in this model, counting of multiple CD4 receptors in a given area is considered to be purely due to chance, i.e., to random statistical fluctuations.

Our imaged CD4 clusters had radii (r i ) in the range approximately 10-100 nm (considering their equivalent circular areas). Their corresponding mean parameters (λ i ) are therefore in the range 0.1-6. The Poisson distribution has significantly different shapes for these different values of its mean parameter, and is increasingly asymmetrical for decreasing values of λ i below 5. For the larger cluster sizes (larger λ i ), the distribution mean (and peak position) shifts to higher values of k and the probability of counting a larger number of molecules per cluster increases. For these reasons, our model considered the different sizes of all the measured clusters to calculate the overall probability distribution of numbers of molecules per cluster that would be observed when counting receptor numbers in circular cluster areas equivalent to those occupied in our measured CD4 clusters.

The expected overall distribution of numbers of molecules per cluster is therefore the average of the Poisson distributions corresponding to all our observed cluster sizes (areas of radius r i ). The overall probability (p) of counting k receptors is:

where we summed over all the different clusters (i is the cluster index and N is the total number of clusters measured), P(k;λ i ) is the Poisson distribution for a given cluster i with radius r i (that occupies a surface area A i = πr 2 i ), and λ i = nA i = πr i 2 n is the corresponding mean value of the Poisson distribution for cluster i.

In order to compare expected and measured distributions, we re-normalised our expected distributions by excluding and dividing by the sum of the remaining counts. This is because areas with zero receptors were not measured in our experiments. Calculations were performed using custom-written Python scripts.

All data are presented as means ± standard deviation (SD) from three independent experiments. N is indicated in each figure separately. Student t-tests were performed by GraphPad Prism 8 (Prism Software). Significance was calculated using unpaired Student's t tests.

In order to study the effect of HIV-binding on the organisation of CD4 molecules at the cell surface, we first established an experimental and analytical framework to quantitatively characterise nanoscale receptor organisation (Figure 1 ). To avoid potential artefacts caused by fixation and antibody-induced artificial crosslinking, we developed an experimental assay in which immunolabeling and receptor-virus binding were carried out at low temperature to minimise membrane fluidity and trafficking [42] [43] [44] . Although HIVtarget cell-binding occurs at 37 • C in vivo, virus bound at 4 • C can display CD4-specific association and occupy an intermediary state in the cell entry process referred to as a temperature arrested state (TAS) [42] [43] [44] . A subsequent brief release of the temperature block, and rapid cooling prior to fixation, allowed us to capture receptor reorganisation at early stages following virus binding ( Figure 1AI ). We have previously carefully evaluated cell fixation protocols to preserve native membrane protein organisation [35] . Labelling of cell-surface receptors is challenging due to the potential for various labelling strategies to subtly alter the membrane organisation of the receptor of interest. We adopted an immunolabelling approach expected to minimise antibody-induced CD4 dimerisation, and confirmed that antibody binding did not lead to detectable crosslinking or perturbation of receptor distribution on the time scales of our assays ( Figure S1 ). We used TIRF-STORM to image CD4 in the cell membrane adjacent to the glass ( Figure 1AII , Figure S2 , estimated localisation precision σ x,y approximately. 15 nm, and Figure S3 ), followed by Clus-Doc [38] to identify clusters and estimate their shape parameters (area, equivalent diameter, and localisation density; Figure 1AIII ). The diameter results produced by Clus-Doc [38] were validated by comparison with manual cluster annotation, confirming the suitability of this approach ( Figure S5 ). TIRF-STORM imaging and cluster characterisation in untreated SupT1-R5 cells revealed CD4 is arranged in small clusters of 64 nm ± 33 nm diameter ( Figure 1B,C) , with the majority (approximately 70%) composed of 1 to 4 CD4 molecules ( Figure 1D and Figure S4 ).

To determine if our analysis pipeline could robustly identify changes in CD4 organisation, we used chemical induction to redistribute CD4 in a predictable and controllable manner. Treatment with the phorbol ester PMA stimulates clathrin-mediated endocytosis (CME) of CD4 by activation of a phosphorylation-dependent dileucine signal [45] . SupT1-R5 cells were treated with PMA for 15 min, as previously described [46] , and imaged with TIRF-STORM. Quantification of CD4 localisations revealed the formation of large plasma membrane CD4 clusters (93 nm ± 36 nm diameter; Figure 2A ,B). At the single cluster level, a clear pool of larger CD4 clusters of up to 800 nm diameter was seen ( Figure 2C ). Increased cluster diameters were not detected in cells fixed immediately following PMA addition, nor in those treated with non-stimulatory 4α-PMA ( Figure 2B,C) , both of which showed cluster diameter distributions similar to that of untreated cells (as in Figure 1C ). Localisation-based estimates indicated that PMA induced an increase in the number of CD4 molecules per cluster (21-100 molecules per cluster; average of 38 ± 73) and, strikingly, a small number of clusters with as many as 500 molecules ( Figure 2D ). This CD4 reorganisation is an expected consequence of PMA treatment and indicates that our pipeline should detect potential HIV-induced changes in the cell-surface CD4 distribution. 

Although HIV cell entry is dependent on the initial binding of CD4 molecules by the viral Env subunit gp120, how receptor binding impacts on the nanoscale organisation of CD4 is poorly understood [2] . Therefore, we set out to measure the effect of virus binding on CD4 nanoscale organisation using our quantitative pipeline.

We incubated SupT1-R5 cells with HIV JR-CSF under CD4-binding permissive ( Figure 3A ) or non-permissive ( Figure 3B ) conditions (i.e., in the presence of the neutralizing anti-CD4 antibody Q4120, which inhibits Env-CD4 binding [47] ), followed by brief treatment at 37 • C before rapid cooling prior to fixation. Dual-colour TIRF-STORM imaging of immunolabeled CD4 and HIV p24 ( Figure 3A ,B) revealed enlarged CD4 clusters around HIV contact sites in permissive conditions, but not in non-permissive control conditions ( Figure 3D,E) . Using coordinate-based colocalisation analysis built into Clus-DoC, we identified and characterised CD4 clusters that were associated with cell-surface bound HIV. HIV-colocalised CD4 clusters were more than twice the diameter of non-HIV-colocalised clusters on the same cells (191 ± 6 nm vs. 71 ± 8 nm, respectively; Figure 3D ). The diameters of non-associated clusters were in good agreement with those measured on untreated cells (65 ± 9 nm vs. 64 ± 33 nm, respectively). This increase in cluster diameter was not observed when HIV binding was blocked by Q4120 (65 ± 6 nm), nor in HIV-bound cells that were fixed prior to 37 • C incubation (63 ± 8 nm) ( Figure 3B ,E and Figure S6 ). In conditions permissive for receptor binding, the increase in CD4 cluster diameter was accompanied by an equivalent increase in the number of CD4 molecules per cluster; 70% of clusters consisted of 13-16 molecules, compared to 1-4 molecules per HIV-associated cluster when receptor binding was inhibited ( Figure 3F ). These results were further validated by HIV-CD4 colocalisation analysis which showed that around 80% of HIV particles were engaged with CD4 clusters in permissive conditions ( Figure S7 ). In contrast, less than 5% of viral particles were associated with CD4 clusters in non-permissive conditions in the presence of Q4120 ( Figure S7B ). This HIV-associated change in clustering was dependent on incubation at 37 • C after release of the TAS (Figure S6 ), and the CD4 cluster changes were abrogated by inhibition of HIV-CD4 interactions ( Figure 3B,E) . These data indicate that HIV-receptor binding at the plasma membrane induced an increase in local CD4 clustering around bound viral particles in a manner dependent on direct interaction between the virus and receptors. Importantly, this reorganisation did not lead to a statistically significant general increase in the density of CD4 clusters across the fields imaged ( Figure S8A ). The increase in cluster diameters is therefore likely to be due to local accumulations of CD4 molecules ( Figure 3C and Figure S8 ).

Next, we compared the changes in CD4 organisation induced by HIV to a theoretical Poisson statistical model used to calculate the distribution of the numbers of molecules per cluster that could be expected from a random distribution of receptors on the cell surface. An overall CD4 surface density (n) of 200 molecules/µm 2 was used, based on the number of CD4 molecules per cell reported for SupT1 cells [40, 41] , and the different cluster sizes measured experimentally were used to generate an averaged theoretical Poisson distribution (see Figure 4A and Section 2). In untreated cells, the experimental data was similar to the modelled distribution ( Figure 4B ). However, in samples with HIV bound under permissive conditions, the experimentally measured data showed a clear additional peak at 13-16 molecules per cluster that cannot be explained by the theoretical random distribution model. Specifically, we measured an approximately four-fold increase in the fraction of clusters with 13-16 CD4 molecules when HIV was present ( Figure 4C) , with that fraction corresponding to an increase in cluster diameters ( Figure S8C ). We also tested the effect of possible variations in cell-surface CD4 levels or cell size by generating theoretical Poisson distributions as above, using overall density values of n = 60 molecules/µm 2 and n = 300 molecules/µm 2 . When we tested our experimental data against these models, we saw a similar clear additional peak at 13-16 molecules per cluster that was not predicted by the theoretical model ( Figure S9 ). These results support the notion that the observed CD4 cluster reorganisation is a result of HIV binding. 

Our data suggest that HIV binding induces a reorganization of CD4 at the cell surface, promoting local concentrations of CD4 molecules, as indicated by an increase in CD4cluster diameters and CD4 molecules per cluster. Based on these findings we posited that the increased CD4 clustering could be a biological requirement for productive HIV binding and/or entry. We sought to explore this hypothesis by assessing the ability of HIV Env to locally recruit CD4 using a reductionist approach. For this purpose, we analysed CD4 organisation following soluble gp120 binding, using a similar approach to that described above for HIV binding. TIRF-STORM imaging and cluster characterisation of CD4 on gp120-treated SupT1-R5 cells revealed increased cluster diameters following the release of the temperature block (84 ± 12 nm) ( Figure 5A (left) , Figure 5B ), although to a lesser extent than that induced by intact HIV (191 ± 6 nm) ( Figure 3D ). This change in cluster diameters was accompanied by a higher variation in CD4 molecules per cluster-a decrease in the number of the smallest clusters of up to 4 molecules, and small increases in the number of clusters consisting of 5-8 and 13-16 molecules ( Figure 5C ). This indicated that upon binding, gp120 alone can locally influence CD4 distribution, although the extent of this effect was less than that induced by viral particles.

CD4 binding by gp120 in functional Env trimers stabilises an 'open' Env conformation that permits subsequent Env binding to a co-receptor-e.g., CCR5-which, in turn, drives membrane fusion and viral entry [43, 48] . To determine whether the changes observed in cell-surface CD4 organisation following HIV binding were dependent on the presence of the co-receptor, in this case CCR5, we compared CD4 clustering on gp120-bound SupT1-R5 cells to that on SupT1 cells which do not express CCR5. No difference was detected in mean cluster diameter (86 ± 18 nm vs. 84 ± 12 nm), or in the number of CD4 molecules per cluster, in the presence or absence of CCR5 ( Figure 5A (right) , Figure 5B ). These results suggest that local recruitment of CD4 molecules immediately following gp120 binding is not dependent on the presence of a co-receptor. 

Knowledge of the initial stages of virus infection i.e., the molecular details underpinning the interaction between viral proteins and host-cell receptors, is essential for a full understanding of the mechanism through which HIV enters cells. While HIV probably first attaches to cells through diverse interactions with various adhesion factors [49] [50] [51] [52] [53] , the virus is dependent on binding to CD4 and the co-receptors CCR5 or CXCR4 for successful fusion with the target cell membrane [54] . Previous studies have pointed to a role for both Env and CD4 clustering in HIV entry [16] [17] [18] [19] [20] [55] [56] [57] , however, the relationship between the spatial distribution of receptors and the fusion process has remained largely speculative. To understand this process in more detail, we sought to characterise the nanoscale organisation of cell surface HIV receptor proteins during virus binding and entry. Here we describe the use of super-resolution SMLM and a suite of analytical tools to determine the organisation of CD4 in response to HIV binding.

We established an experimental and analytical pipeline with the necessary range and sensitivity to detect nanoscale receptor organisation. Although SMLM provides significantly improved lateral and axial spatial resolution, most membrane receptor studies are conducted via two-dimensional imaging and neglect cell-surface nano-topography. Immobilisation of cells on a glass surface for imaging in TIRF mode can sometimes interfere with the complex cell-surface morphology. For instance, the flattening of T cells on PLLcoated glass can cause artefacts when studying membrane receptor (re)organisation [58] [59] [60] . In our study, we co-incubated HIV and CD4 + T cells in suspension before depositing the cells on PLL-coated coverslips to minimise potential artefacts induced by cell-glass interactions.

To verify that our approach could identify nanoscale changes in CD4 organisation, we tested this pipeline using PMA, a known inducer of CD4 clustering and clathrinmediated CD4 endocytosis [46] (Figures 1 and 2) . Density-based nanoscale analysis of CD4 organisation on untreated T cells revealed that CD4 is distributed into nanoscale domains containing a single molecule or small clusters of up to four CD4 molecules ( Figure 1 ). These results agree with a growing body of evidence that cell-surface receptors are generally organised into clusters [5, 25, 48, 61] , some on a scale similar to membrane nanodomains (approximately 50-200 nm), that may correspond to the corrals organised by the cortical actin meshwork [62] [63] [64] . However, the term 'cluster' can be challenging to define in SMLM because detected localisations could represent a single molecule or small group of molecules. We adopted a definition from similar work that studied TCR distributions, specifically that nanoclusters are 'molecules grouped at a sub-diffraction limit scale (<250 nm), such that the grouped coordinates are unlikely to be completely randomly distributed' [65] .

Using SMLM localisation, we were able to obtain estimates of the numbers of CD4 molecules per localisation or cluster, following the approach described by Sieben et al. [31] . Considering that the numbers of molecules counted was mostly <10 per cluster, it is important to note that these figures should be considered as rough estimates and not true numbers of molecules. The accurate determination of molecule numbers using SMLM methods remains a challenge due to labelling efficiency, re-emitting fluorophores, variable imaging conditions and differences in analytical approaches [66] . A potential undercounting in our experiments may arise from low labelling efficiency. In particular, we may underestimate the number of CD4 molecules as OKT4, the anti-CD4 antibody used in this work, has a lower binding efficiency compared to, for example, the neutralizing anti-CD4 antibody Q4120. Q4120 is more commonly used for characterising cell-surface CD4 but is unsuitable for HIV-CD4 interaction studies. In addition, we cannot completely exclude the possibility of CD4 dimerisation by the labelling antibody which, although we would expect it to be minimal, may also contribute to receptor undercounting. Although the CD4 levels per cell measured in this study (total number of molecules/the area of a region of interest) are lower than those reported previously (60 molecules/µm 2 vs. 200 molecules/µm 2 ) [40, 41] , given the very different measuring techniques used, the numbers are reasonably close. The post-processing calibration for molecule counting implemented here may also lead to undercounting. The process depends on the gap time distribution parameter selected when quantifying the dark time used to calibrate the experimental localisation coordinate lists. The gap time distribution fitting is a trade-off between over-merging (i.e., high confidence but underestimated protein count) and under-merging (i.e., lower confidence but higher protein count). In this study, a longer gap time was used to minimise false-positive molecular identifications. Despite these uncertainties, we can nevertheless extract useful information by emphasising the relative changes in molecule numbers under different experimental conditions. Our results consistently indicate a 3-to-10-fold increase in both cluster diameter and the number of molecules assembled around bound HIV particles (Figure 3) . Interestingly, the persistence of cell-bound HIV not associated with CD4 or in assays with Q4120 can be attributed to the ability of HIV to bind to cells through interactions with adhesion factors, including integrins and lectins [49] [50] [51] [52] [53] .

To further confirm that the CD4 clustering detected on cells with bound HIV was not attributable to chance, we applied statistical modelling to simulate a random distribution of receptors on the cell surface and compared our results with the experimental data ( Figure 4 ). Based on previously reported numbers of approximately 100,000 CD4 molecules/SupT1 cell [40, 41] , and an average T-cell radius of 10 µm, we assumed an overall mean density (n) of 200 CD4 molecules/µm 2 in our model ( Figure 4) . To compare this estimate with our experimental data, we estimated the number of CD4 molecules on SupT1-R5 cells labelled with Q4120 and OKT4. We measured a density of 180 molecules/µm 2 with Q4120 and 60 molecules/µm 2 with OKT4. Given these antibodies recognise different epitopes on the CD4 molecule, we regard these measurements as being in reasonable agreement. Nevertheless, to take possible variations in detected CD4 levels and in cell size into account, we tested our model at lower and higher mean density values of n = 60 molecules/µm 2 and n = 300 molecules/µm 2 ( Figure S9 ). In all cases, our conclusions stand, i.e., HIV binding induces CD4 clustering, as opposed to clustering being a product of occasional random accumulation. This statistical model allows us to assign greater confidence to our interpretation of the data and the biological significance of receptor clustering changes. Importantly, this framework can be easily adapted to studies of other membrane-associated receptors and induced organisational changes.

Given the measured density of CD4 molecules within the HIV-induced clusters (approximately 500 molecules/µm 2 ), we estimated the inter-molecular centre-to-centre distances for CD4 molecules as 40-50 nm. This distance is larger than the measured width of trimeric Env complexes of 10-15 nm [17, 20, 67, 68] . Even considering the likely undercounting in our data, this suggests that not all gp120 molecules on an individual Env spike engage CD4. Further, given that mature HIV particles have an average of 10 Env trimers per virion [13] [14] [15] , which can be arranged in clusters in the viral envelope [12, 55] . Our measured numbers of 13-16 CD4 molecules in an HIV-associated cluster suggests an approximate ratio of 1:1 CD4 per Env trimer. Nevertheless, it remains difficult to estimate the precise Env-CD4 stoichiometry required for fusion and entry, as the organisation of the relevant Env-CD4 interactions within each virus-CD4 cluster remains to be established. For membranes to fuse, both CD4 and Env molecules, as well as other proteins, must be cleared from the zone of close approach of the opposing bilayers. Thus, CD4-Env interactions might be organised as a ring at the edge of the cell-virus interface. In part at least, this would be consistent with the observation that the measured CD4 clusters have diameters that exceed those of virus particles (typically approximately 120 nm) and point to a larger biophysical influence for Env trimer clustering than previously thought, potentially requiring flexibility in Env and/or CD4 as recently shown for the spike (S) protein of SARS-CoV-2 [69] .

From a downstream mechanistic point of view, although signalling through the GPCR co-receptors has been identified as a process that HIV can exploit to remodel the cytoskeleton and facilitate productive infection [68] , there are conflicting reports as to whether GPCR-dependent signalling through CCR5 is required for HIV entry [70, 71] . The CD4 clustering we observed, coupled with the independence of gp120-induced CD4 clustering from co-receptor CCR5, suggests that HIV-induced signalling through CD4 and Lck (such as that detected by Lucera et al. [71] ) can trigger CD4 clustering beyond the range of Env binding. Furthermore, CD4/Lck signalling, while not essential for HIV infection (macrophages are Lck-negative yet HIV-susceptible), may play a more significant role in facilitating efficient HIV entry in some cell types than previously appreciated. A few theoretical studies have used stochastic models that account for receptor diffusion and receptor-virus binding to predict the accumulation of HIV receptors at virus-binding sites on the surface of target cells [72] [73] [74] . Future comparison of model predictions and experimental data may provide further insights into the mechanisms of receptor cluster formation.

In summary, our study provides one of the first nanoscale analysis of HIV-induced spatial changes in cell-surface CD4 distribution during the initial steps of HIV infection on CD4 + T cells. HIV-induced CD4 spatial reorganisation might link to their functional role during HIV binding and fusion. This work contributes to our goal of better understanding how viruses spatially modulate cell-surface receptors to facilitate their entry, and how we might prevent virus infection by inhibiting not only receptor binding but also receptor spatial redistribution. The imaging and analytical pipeline we have established provides a powerful and robust toolbox to further study HIV entry as well as the properties of other virus-receptor systems and membrane proteins in general.

Supplementary Materials: The following are available online at https://www.mdpi.com/1999-491 5/13/1/142/s1, Figure S1 . Quantification of CD4 cluster diameters after releasing the temperature block, Figure S2 . Localisation maps for STORM imaging data. Figure S3 . Experimentally obtained localisation precision σ for Alexa Fluor 647. Figure S4 . Calibration data for molecule counting from STORM data. Figure S5 . Comparison of CD4 cluster diameters distributions determined by automated Clus-DoC analysis and manual measurements. Figure S6 . CD4 does not cluster on HIV-1 treated cells at 4 • C. Figure S7 . Analysis of CD4 clusters colocalised with HIV. Figure S8 . Diameters and densities of CD4 clusters on HIV-treated and untreated cells. Figure S9 . Statistical modelling of CD4 surface distribution. 

The cell biology of receptor-mediated virus entry

A biophysical perspective on receptor-mediated virus entry with a focus on HIV

Signaling clusters in the cell membrane

The coreceptor CD4 is expressed in distinct nanoclusters and does not colocalize with T-cell receptor and active protein tyrosine kinase p56lck

Lat are expressed on separate protein islands on T cell membranes and concatenate during activation

HIV requires multiple gp120 molecules for CD4-mediated infection

Cooperation of Multiple CCR5 Coreceptors Is Required for Infections by Human Immunodeficiency Virus Type 1

An Allosteric Rheostat in HIV-1 gp120 Reduces CCR5 Stoichiometry Required for Membrane Fusion and Overcomes Diverse Entry Limitations

Single-Molecule Analysis of Human Immunodeficiency Virus Type 1 gp120-Receptor Interactions in Living Cells

Monitoring Early Fusion Dynamics of Human Immunodeficiency Virus Type 1 at Single-Molecule Resolution

Envelope glycoprotein mobility on HIV-1 particles depends on the virus maturation state

Electron tomography analysis of envelope glycoprotein trimers on HIV and simian immunodeficiency virus virions

Distribution and three-dimensional structure of AIDS virus envelope spikes

Envelope Glycoprotein Incorporation, Not Shedding of Surface Envelope Glycoprotein (gp120/SU), Is the Primary Determinant of SU Content of Purified Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency Virus

Moesin is required for HIV-1-induced CD4-CXCR4 interaction, F-actin redistribution, membrane fusion and viral infection in lymphocytes

Filamin-A regulates actin-dependent clustering of HIV receptors

HIV-1 gp120 induces an association between CD4 and the chemokine receptor CXCR4

Membrane raft microdomains mediate lateral assemblies required for HIV-1 infection

Actin-Dependent Receptor Colocalization Required for Human Immunodeficiency Virus Entry into Host Cells

HIV gp120-induced interaction between CD4 and CCR5 requires cholesterol-rich microenvironments revealed by live cell fluorescence resonance energy transfer imaging

Different Infectivity of HIV-1 Strains Is Linked to Number of Envelope Trimers Required for Entry

Super-resolution fluorescence microscopy studies of human immunodeficiency virus

Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)

Organization of the resting TCR in nanoscale oligomers

Super-resolution characterization of TCR-dependent signaling clusters

TCRs are randomly distributed on the plasma membrane of resting antigen-experienced T cells

A Density-Based Algorithm for Discovering Clusters in Large Spatial Databases with Noise

Coordinate-based colocalization analysis of single-molecule localization microscopy data

Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination

Super-resolution imaging reveals the nanoscale organization of metabotropic glutamate receptors at presynaptic active zones

Influenza A viruses use multivalent sialic acid clusters for cell binding and receptor activation

Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging

ERM-Dependent Assembly of T Cell Receptor Signaling and Co-stimulatory Molecules on Microvilli prior to Activation

Separation of functional subsets of human T cells by a monoclonal antibody

Henriques, R. Fix Your Membrane Receptor Imaging: Actin Cytoskeleton and CD4 Membrane Organization Disruption by Chemical Fixation

ThunderSTORM: A comprehensive ImageJ plug-in for PALM and STORM data analysis and super-resolution imaging

Clus-DoC: A combined cluster detection and colocalization analysis for single-molecule localization microscopy data

An open-source platform for biological-image analysis

Differential endocytosis of CD4 in lymphocytic and nonlymphocytic cells

Quantification of CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages

Temperature dependence of cell-cell fusion induced by the envelope glycoprotein of human immunodeficiency virus type 1

The temperature arrested intermediate of virus-cell fusion is a functional step in HIV infection

Time-resolved imaging of HIV-1 Env-mediated lipid and content mixing between a single virion and cell membrane

Cluster of differentiation antigen 4 (CD4) endocytosis and adaptor complex binding require activation of the CD4 endocytosis signal by serine phosphorylation

Phorbol Ester-induced Downregulation of CD4 is a Multistep Process Involving Dissociation from p56 lck, Increased Association with Clathrin-coated Pits, and Altered Endosomal Sorting

CD4-Chemokine Receptor Hybrids in Human Immunodeficiency Virus Type 1 Infection

Associating HIV-1 envelope glycoprotein structures with states on the virus observed by smFRET

HIV-1 envelope protein binds to and signals through integrin α4β7, the gut mucosal homing receptor for peripheral T cells

The integrin α4β7 forms a complex with cell-surface CD4 and defines a T-cell subset that is highly susceptible to infection by HIV-1

Syndecans Serve as Attachment Receptors for Human Immunodeficiency Virus Type 1 on Macrophages

DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells

HIV: Cell binding and entry. Cold Spring Harb

A General Model for the Transmembrane Proteins of HIV and Other Retroviruses

Maturation-dependent HIV-1 surface protein redistribution revealed by fluorescence nanoscopy

Dynamic reorganization of chemokine receptors, cholesterol, lipid rafts, and adhesion molecules to sites of CD4 engagement

A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction

Adhesion of cells to surfaces coated with polylysine: Applications to electron microscopy

Supported planar bilayers in studies on immune cell adhesion and communication

Capturing resting T cells: The perils of PLL correspondence

The Size of Activating and Inhibitory Killer Ig-like Receptor Nanoclusters Is Controlled by the Transmembrane Sequence and Affects Signaling

Plasma membrane-associated proteins are clustered into islands attached to the cytoskeleton

Multi-protein assemblies underlie the mesoscale organization of the plasma membrane

Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane

Can single molecule localization microscopy detect nanoclusters in T cells?

Blinking Statistics and Molecular Counting in direct Stochastic Reconstruction Microscopy (dSTORM). bioRxiv 2019, 834572

GPCR signalling from within the cell

HIV Envelope-CXCR4Signaling Activates Cofilin to Overcome Cortical Actin Restriction in Resting CD4 T Cells

In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges

HIV signaling through CD4 and CCR5 activates Rho family GTPases that are required for optimal infection of primary CD4+ T cells

Protein-Dependent CCR5 Signaling Is Not Required for Efficient Infection of Primary T Lymphocytes and Macrophages by R5 Human Immunodeficiency Virus Type 1 Isolates

Diffusion-Dependent Mechanisms of Receptor Engagement and Viral Entry

Organization of Cellular Receptors into a Nanoscale Junction during HIV-1 Adhesion

Role of Gp120 Trimerization on HIV Binding Elucidated with Brownian Adhesive Dynamics

We thank James A. Hoxie for providing HEK293T, SupT1 and SupT1-R5 cell lines. We thank Christian Sieben for sharing the MATLAB scripts and helpful discussions on molecule counting. We also thank the MRC LMCB Light Microscopy Facility for support.

The authors declare no conflict of interest.