key: cord-0920553-ben1dy73
authors: Masi, Marco; Di Lecce, Roberta; Mérindol, Natacha; Girard, Marie-Pierre; Berthoux, Lionel; Desgagné-Penix, Isabel; Calabrò, Viola; Evidente, Antonio
title: Cytotoxicity and Antiviral Properties of Alkaloids Isolated from Pancratium maritimum
date: 2022-04-07
journal: Toxins (Basel)
DOI: 10.3390/toxins14040262
sha: 926cc872f0fe45fda5799cbc0091ab2796059bce
doc_id: 920553
cord_uid: ben1dy73

Ten Amaryllidaceae alkaloids (AAs) were isolated for the first time from Pancratium maritimum collected in Calabria region, Italy. They belong to different subgroups of this family and were identified as lycorine, which is the main alkaloid, 9-O-demethyllycorine, haemanthidine, haemanthamine, 11-hydroxyvittatine, homolycorine, pancracine, obliquine, tazettine and vittatine. Haemanthidine was isolated as a scalar mixture of two 6-epimers, as already known also for other 6-hydroxycrinine alkaloids, but for the first time they were separated as 6,11-O,O′-di-p-bromobenzoyl esters. The evaluation of the cytotoxic and antiviral potentials of all isolated compounds was undertaken. Lycorine and haemanthidine showed cytotoxic activity on Hacat cells and A431 and AGS cancer cells while, pancracine exhibited selective cytotoxicity against A431 cells. We uncovered that in addition to lycorine and haemanthidine, haemanthamine and pancracine also possess antiretroviral abilities, inhibiting pseudotyped human immunodeficiency virus (HIV)−1 with EC50 of 25.3 µM and 18.5 µM respectively. Strikingly, all the AAs isolated from P. maritimum were able to impede dengue virus (DENV) replication (EC(50) ranged from 0.34–73.59 µM) at low to non-cytotoxic concentrations (CC(50) ranged from 6.25 µM to >100 µM). Haemanthamine (EC50 = 337 nM), pancracine (EC(50) = 357 nM) and haemanthidine (EC(50) = 476 nM) were the most potent anti-DENV inhibitors. Thus, this study uncovered new antiviral properties of P. maritimum isolated alkaloids, a significant finding that could lead to the development of new therapeutic strategies to fight viral infectious diseases.

Plants belonging to the Amaryllidaceae family are distributed in Northern and Southern hemispheres, but they essentially grow in Andean South America, in the Mediterranean This study reports the identification of ten alkaloids isolated from the acid bulb extract of P. maritimum and for the first time describes the separation of the two 6-epimers of haemanthidine as p-bromobenzoyl derivatives, which also allowed us to assign their relative and absolute configuration. The results of their cytotoxic and antiviral properties were also described.

Dried and minced P. maritimum bulbs were acid extracted and the aqueous phase was re-extracted with EtOAc following alkalinization, as detailed in Experimental section. Evaporation of the solvent left an oily residue which was crystallized and yielded an abundant amount of lycorine (1, Figure 2 ), the main isolated metabolite. The mother liquors resulting from lycorine crystallization were evaporated under reduced pressure, yielding a residue which was purified using a combination of column and TLC chromatography on silica gel, allowing us to isolate ten already known AAs identified as tazettine, homolycorine, obliquine, vittatine, haemanthamine, haemanthidine, 9-Odemethylhomolycorine, 11-hydoxyvittatine and pancracine (2-10, Figure 2 ), thus belonging to lycorine-(1), pretazettine-(2), homolycorine-(3, 4 and 8), crinine-(5-7 and 9) and pancracine-(10) types. 

Dried and minced P. maritimum bulbs were acid extracted and the aqueous phase was re-extracted with EtOAc following alkalinization, as detailed in Experimental section. Evaporation of the solvent left an oily residue which was crystallized and yielded an abundant amount of lycorine (1, Figure 2 ), the main isolated metabolite. The mother liquors resulting from lycorine crystallization were evaporated under reduced pressure, yielding a residue which was purified using a combination of column and TLC chromatography on silica gel, allowing us to isolate ten already known AAs identified as tazettine, homolycorine, obliquine, vittatine, haemanthamine, haemanthidine, 9-O-demethylhomolycorine, 11hydoxyvittatine and pancracine (2-10, Figure 2 ), thus belonging to lycorine-(1), pretazettine-(2), homolycorine-(3, 4 and 8), crinine-(5-7 and 9) and pancracine-(10) types.

These alkaloids were identified comparing their physical (specific optical rotation) and spectroscopic ( 1 H and 13 C NMR and ESI MS) properties with those already reported in the literature: for 1 by Lamoral-Theys et al. (2009) [13] isolated from Stenbergia lutea, for 2 with those reported by Antoun et al. (1993) [41] and Boit and Döpke (1960) [42] when isolated from Hymenocallis expansa and Hippeastrum aulicum, respectively. Alkaloids 3 and 4 were identified by comparing our experimental results with those reported by Fales et al. (1956) [43] , Furosawa et al. (1976) [44] and Baxendale and Lei (2005) [45] when isolated from Narcissus tazetta and Cyrtanthus obliquus. Alkaloids 5 and 6 were identified comparing our data with those reported by Frahm et al. (1985) [46] , Bastida et al. (1995) [47] , Pabuççuoglu et al. (1989) [48] and Ghosal et al. (1985) [49] when isolated from Crinum asiaticum, Narcissus cantabricus and Sternbergia sicula, respectively. Likewise, alkaloids 7 and 8 were identified by comparing their data with those described by Ma et al. (1986) [50] , Antoun et al. (1993) [41] , Forgo and Hoffmann (2005) [51] , Bastida et al. (1987) [52] and Evidente et al. (1994) [53] when isolated from Hymenocallis expansa, Narcissus tazetta L. var chinensis roem, Leucojum vernum and Narcissus confusus, respectively. Finally, alkaloids 9 and 10 were identified by comparison with those reported by Evidente (1986) [54] and Ali et al. (1984) [55] when isolated from Hippeastrum vittatum and Stenbergia lutea, respectively. Interestingly, among all the alkaloids isolated in this study, obliquine (4) and pancracine (10) were isolated for the first time from P. maritimum. Figure 2 . Structures of lycorine, tazettine, homolycorine, obliquine, vittatine, haemanthamine, haemanthidine, 9-O-demethylhomolycorine, 11-hydroxyvittatine, pancracine (1-10) isolated from P. maritimum and that of the two diastereomeric p-bromobenzoyl esters of 7 (11 and 12).

These alkaloids were identified comparing their physical (specific optical rotation) and spectroscopic ( 1 H and 13 C NMR and ESI MS) properties with those already reported in the literature: for 1 by Lamoral-Theys et al. (2009) [13] isolated from Stenbergia lutea, for 2 with those reported by Antoun et al. (1993) [41] and Boit and Döpke (1960) [42] when isolated from Hymenocallis expansa and Hippeastrum aulicum, respectively. Alkaloids 3 and 4 were identified by comparing our experimental results with those reported by Fales et al. (1956) [43] , Furosawa et al. (1976) [44] and Baxendale and Lei (2005) [45] when isolated from Narcissus tazetta and Cyrtanthus obliquus. Alkaloids 5 and 6 were identified comparing our data with those reported by Frahm et al. (1985) [46] , Bastida et al. (1995) [47] , Pabuççuoglu et al. (1989) [48] and Ghosal et al. (1985) [49] when isolated from Crinum asiaticum, Narcissus cantabricus and Sternbergia sicula, respectively. Likewise, alkaloids 7 and 8 were identified by comparing their data with those described by Ma As previously reported, haemanthidine (7) was isolated as two 6-epimers [56] in a scalar ratio. The equilibrium between the two epimers was also reported for 6-hydroxycrinamine, but not for closely related crinine-type alkaloids such as 6α-hydroxycrinine and 6αhydroxybuphonisine. The equilibrium between the two epimers could be explained through their interconversion in solution via the corresponding aminoaldehyde [57] . The ratio of the two epimers in solution, evidenced by 1 H NMR spectroscopy, is affected from the variable temperature at which the spectrum is recorded. Haemanthidine (7) epimeric ratio was of 2:1, whereas King et al. (1965) [57] observed a 1:1 ratio. Any attempt to separate the two 6-epimers and to determine their absolute configuration failed. In addition, their esters were not separable upon converting the mixture of 6-epimers in the corresponding 6,11-O,O -diacetyl derivatives. Instead, when converted by reaction with p-bromobenzoyl chloride in the usual conditions, haemanthidine yielded two corresponding epimeric esters (11 and 12, Figure 2 ) which were easily separated by TLC chromatography and characterized by physic properties (specific optical rotation), 1 H NMR and ESI MS spectroscopy. The comparison of the two 1 H NMR spectra (Table 1 ) of 11 and 12 with that of 7 showed as substantial difference the presence of the signal pattern of a p-disubstituted phenyl residue, appearing as two couples of doublets (J = 8.2 and J = 8.6 Hz) at δ 7.93 and 7.60 (2 ,6 and 3 ,5 ) and 7.77 and 7.58 (2 ,6 and 3 ,5 ) for 11, and (J = 8.4 and J = 8.7 Hz) at δ 8.00 and 7.62 (2 ,6 and 3 ,5 ), and 7.77 and 7.59 (2 ,6 and 3 ,5 ) for 12. Furthermore, the expected significant downfield shifts H-6 and H-11 were observed. In particular, in the spectrum of 11, H-6 and H-11 appeared as a singlet and a double doublets (J = 7.6 and 3.6 Hz) downfield shifted (∆δ 1.27 and 1.23) with respect to 7 at δ 6.38 and 5.18, similarly in 12, the same signal appeared as a singlet (H-6) and a double doublet (J = 6.9 and 2.7 Hz) (H-11) downfield shifted (∆δ 1.18 and 1.25) with respect to 7 at δ 6.93 and 5.20, respectively. Furthermore, 11 and 12, as expected showed the same typical ESI MS spectrum exhibiting the three peaks due the presence of the two isotopes of the two bromine at m/z

The NOESY spectra (Table 2 ) of 11 and 12 showed both the expected correlations of H-1 with H-10 and H-2, H-2 with H-3, the latter with H 2 -4 and MeO, H-11 with H-12A and the latter with H-12B. There was a significant difference with regards to the correlation observed in 12 between H-6 and H-4a, which was absent in 11. This last result allowed us to assign the same β-orientation of H-6 as H-4a in 12, while in 11 H-6 resulted to be α-located. Thus, knowing the absolute configuration of other chiral centers of haemanthidine [58] , H-6 has a S-configuration in 12 and an R-configuration in 11. All the attempts to recovert the p-bromobenzoyl esters in the free base 7 by hydrolysis carried out in different conditions failed. Previous literature reported that several AAs, including haemanthidine as a 6-epimer mixture displayed cytotoxic potential against several types of cancer cell lines [59] and the growth inhibitory effect on both parental and multidrug resistant L5178 mouse lymphoma cell lines [60] . Thus, the cytotoxic activity was assessed for all the isolated alkaloids (1-6 and 8-10), the scalar 6-epimer mixture of 7 and their two ester 11 and 12.

The potential cytotoxic activity of P. maritimum alkaloids was evaluated on Hacat, A431 and AGS cells treated with concentrations ranging from 0.5 to 10 µM for 24 and 48 h by measuring the reduction of 3-(4,5-dimethylthiazol-2) 2,5-diphenyltetrazolium bromide (MTT) to formazan by the mitochondrial enzyme succinate dehydrogenase. We selected Hacat cells because they are spontaneously immortalized human keratinocytes and they have been used in several studies as a paradigm for non-transformed epidermal cells. A431 and AGS cells were chosen as transformed epithelial cells derived from skin epidermoid tumor and gastric carcinoma, respectively. Interestingly, as shown in Figures 3 and 4 , in addition to lycorine (IC 50 < 0.5 µM), AGS cells were sensitive to haemanthamine (6) (IC 50 = 7.5 µM at 24 and 48 h) and haemanthidine (7) (IC 50 = 5.0 µM at 24 h), albeit at much higher concentrations (Table 3) .

We further evaluated the cytotoxic activity of the two p-bromobenzoyl derivatives (11, 12) of haemanthidine with concentrations ranging from 0.5 to 50 µM for 24 and 48 h. As shown in Figure 4 , both epimers were able to reduce AGS cell viability.

The only alkaloid with cytotoxic activity on Hacat and A431 cells was lycorine (IC 50 = 0.5 µM at 48 h for both cell lines). Indeed, we only observed a slight to moderate reduction of Hacat and A431 cell viability (between 15-30%) with 10 µM haemanthidine, -O-demethylhomolycorine, haemanthamine and tazettine at 24 h of treatment. At 48 h, cell growth was almost completely rescued suggesting that cells underwent a reversible cell cycle arrest. This suggest that haemanthidine and haemanthamine could display a selective toxicity towards cancer cells.

Among the alkaloids isolated from P. maritimum in this study, several have been associated with antiviral functions, while others have not been tested. Lycorine, haemanthamine and 11-hydroxyvittatine were reported to inhibit the replication of influenza virus H5N1 [29] . Haemanthamine, lycorine and homolycorine were also shown to display antiretroviral activities [30] , while lycorine and derivatives are potent inhibitors of flaviviruses and coronaviruses. In this study, we assessed the antiviral activity of all the isolated alkaloids (1-6 and 8-10) and the scalar 6-epimer mixture of 7. We focused on the antiretroviral and anti-flaviviral activities, as we and others have previously shown that several AAs efficiently target HIV-1 and DENV replication ( Figure 5 ). of biological replicates. Each mean was compared using a Dunnett's multiple comparisons test of ANOVA one-way (p-value * < 0.01, ** < 0.05, *** p < 0.001; **** p < 0.0001). DMSO control. thamine and 11-hydroxyvittatine were reported to inhibit the replication of influenza virus H5N1 [29] . Haemanthamine, lycorine and homolycorine were also shown to display antiretroviral activities [30] , while lycorine and derivatives are potent inhibitors of flaviviruses and coronaviruses. In this study, we assessed the antiviral activity of all the isolated alkaloids (1-6 and 8-10) and the scalar 6-epimer mixture of 7. We focused on the antiretroviral and anti-flaviviral activities, as we and others have previously shown that several AAs efficiently target HIV-1 and DENV replication ( Figure 5 ). Tazettine (2) 11-Hydroxyvittatine (9) Obliquine (4) Haemanthamine (6) Haemanthidine (7) Homolycorine (3) Pancracine (10) Lycorine ( To account for non-specific antiviral activity caused by enhanced cell death, the cytotoxicity of P. maritimum isolated AAs was first determined in THP-1 and Huh7 virus-targeted cell lines exposed to concentrations ranging from 0.05 to 200 µM for 72 h. Cell viability was measured using ATP levels as a marker. The most cytotoxic compounds were lycorine, haemanthamine, pancracine, haemanthidine, followed by 11-hydroxyvitattine, tazettine and obliquine in both THP-1 and Huh7 cells ( Figure 5A,B) . In THP-1 cells used for HIV-1 infection, lycorine (CC 50 = 4.6 µM) impacted cell viability at all concentrations whereas haemanthidine (CC 50 = 16.8 µM), haemanthamine (CC 50 = 22.2 µM) and pancracine (CC 50 = 25.93 µM), decreased cell viability beginning at 3.125 µM with 71%, 71% and 82% of live cells at 6.25 µM, respectively. A concentration of 100 µM of obliquine, tazettine and 11-hydroxyvittatine reduced viable THP-1 cells to 55.3%, 60.9% and 52.3%, respectively ( Figure 5A and Table 3 ). In the Huh7 cells used for DENV infection, 0.78 µM of lycorine reduced viability by 21%, while haemanthamine was cytotoxic at concentrations above 3.125 µM (82.2% live cells), and pancracine and haemanthidine decreased viability beginning at 6.25 µM (78.1% and 83.3% of live cells, respectively). 11-hydoxyvittatine and tazettine reduced viability at concentrations higher than 50 µM (73.8% and 85.2% viable cells, respectively) and obliquine was toxic from 100 µM (82.6%) ( Figure 5B ). The AAs CC 50 were not reached at the concentrations used in our assays.

Next, pseudotyped HIV-1 GFP vectors were used to assess the antiretroviral potential of alkaloids. These vectors enter THP-1 cells, reverse transcribe their viral RNA, integrate viral DNA into the host genome, produce viral proteins and GFP, but do not lead to the production of infectious viral particles. We treated THP-1 cells with each AA and infected them at an MOI = 0.1 ( Figure 5C ). The percentage of infected GFP + THP-1 cells was measured 72 h post-infection using a flow cytometer. Lycorine, haemanthamine, pancracine, haemanthidine, 11-hydroxyvittatine, tazettine and obliquine displayed a dose-dependent inhibition of HIV-1. Complete viral inhibition was observed for haemanthidine, lycorine, haemanthamine, and pancracine at 100 µM ( Figure 5C ) with EC 50 of 12.7 µM, 10.9 µM, 25.3 µM, 18.5 µM, respectively ( Figure 5C and Table 4 ). Whereas haemanthamine and lycorine have been previously reported to display antiretroviral activities [30] , our results suggest that haemanthidine and pancracine also share this function. However, and for all four alkaloids, only 40-50% of cells remain viable at the EC 50 . Thus, although these alkaloids display interesting antiretroviral effects as previously reported, the effective concentrations to block infection are considerably cytotoxic. Lycorine is recognized for its anti-flaviviral properties. We assessed AAs' anti-flavivirus properties using the DENV GFP dengue vector, which is propagation-competent. Upon infection, this vector leads to the production of GFP concomitantly with the translation of viral genomic RNA. Infection was measured by assessing the % of GFP + cells 72 h post-infection on a flow cytometer ( Figure 5D ). Obliquine (4) (EC 50 (6) (EC 50 = 0.337 µM) all impeded DENV GFP replication. Complete inhibition was obtained at 1.56 µM for lycorine, haemanthamine, pancracine and haemanthidine. At this concentration, only lycorine was cytotoxic at detectable levels, reducing cell viability by 25% ( Figure 5B,D) . To our knowledge, this is the first report on these AAs inhibiting flavivirus at levels comparable to lycorine and less cytotoxicity. Thus, we have uncovered that Pancratium maritimum's extracted haemanthamine, haemanthidine and pancracine are potent antiflaviviral compounds that should be further explored.

This manuscript reports an in-depth investigation of P. maritimum isolated from the first time in South Italy Coast. Ten already known Amaryllidaceae alkaloids belonging to different subgroups were identified as lycorine, the main alkaloid, 9-O-demethyllycorine, haemanthidine, haemanthamine, 11-hydroxyvittatine, homolycorine, pancracine, obliquine, tazettine and vittatine, with obliquine and pancracine isolated for the first time from this Amaryllidaceae. Lycorine and haemanthidine showed cytotoxic activity on Hacat cells and A431 and AGS cancer cells while, pancracine exhibited selective cytotoxicity against A431 cells. Finally, we uncovered that P. maritimum's isolated haemanthamine, haemanthidine and pancracine display promising antiflaviviral activity.

Optical rotations were measured in a MeOH solution on a Jasco P-1010 digital polarimeter (Jasco, Tokyo, Japan). 1 H and 13 C NMR spectra were recorded at 400 and 100 MHz, respectively, in CD 3 OD or otherwise noted on a Bruker spectrometer (Bruker, Karlsruhe, Germany). The same solvent was used as an internal standard. and Nuclear Overhauser Effect Spectroscopy (NOESY) experiments [61] were performed using Bruker microprograms; ESI mass spectra and liquid chromatography (LC)/MS analyses were performed using the LC/MS TOF system Agilent 6230B, HPLC 1260 Infinity (Agilent, Milam, Italy). The HPLC separations were performed with a Phenomenex LUNA (C18 (2) 

Bulbs of Pancratium maritimum were collected in Squillace, Italy (38 • 47 20.6 N and 16 • 35 10.8 W), in August 2020 (Figure 1 ). The plant specimen is deposited in the collection of the same Department of Biology, University of Naples Federico II.

Fresh bulbs of P. maritimum (6 kg) were dried at room temperature and then finely powdered. The resulting powder (736.2 g) was extracted with 1% H 2 SO 4 , (2 × 1 L) overnight at room temperature. The suspension was filtered through cloth and successively centrifuged at 10 • C at 7000 rpm for 30 min. The acid extract was alkalinized to pH 9-10 with 12 N NaOH. The aqueous solution was extracted with EtOAc (3 × 1 L), and the organic extracts were combined, dried (Na 2 SO 4 ) and evaporated under reduced pressure to give a brown oil residue (3.9 g). This latter was crystallized by EtOH obtaining lycorine (1, 900 mg) as white crystals. The mother liquors of crystallization were dried, and the residue (3.0 g) was fractionated by column chromatography eluted with CHCl 3 -EtOAc-MeOH (2:2:1), affording eight groups of homogeneous fractions (F1-F8). The residue (182.5 mg) of fraction F3 was purified on silica gel column chromatography (CC) eluted with CHCl 3 -i-PrOH (9:1), yielding five fractions (F3.1-F3.5). The residue (50.5 mg) of fraction 2 resulted to be an amorphous solid identified as tazettine (9) . The residue (336.5 a mg) of fraction F4 was purified on CC eluted with EtOAc-MeOH-H 2 O (7:2:1) yielding seven homogeneous fractions (F4.1-F4.7). The residue (48.9 mg) of F4.1 was further purified by preparative TLC eluted with CHCl 3 -EtOAc-MeOH (2:2:1) obtaining an amorphous solid identified as homolycorine (6, 8.4 mg) . The residue (15.6 mg) of F4.3 was purified by TLC eluted with CHCl 3 -EtOAc-MeOH (2:2:1) yielding obliquine (8, 4. Hemanthidine, (7) (2.0 mg), was dissolved in CH 3 CN (600 µL), and 4-dimethylaminopyridine (DMAP) (4.3 mg) and p-bromobenzoyl chloride (4.1 mg) were added. The reaction mixture was stirred at room temperature for 24 h and then evaporated under reduced pressure. The residue (10.2 mg) was purified by TLC on silica gel, eluent n-hexane-EtOAc (6:4) giving Derivatives 11 (2.3 mg), and 12 (3.8 mg), as colorless oils. [64] vectors, both expressing the green fluorescent protein (GFP), were used to investigate alkaloids' antiviral properties. The multiplicity of infection (MOI) of HIV-1 GFP was assessed by measuring the infectivity of serially diluted vector preparation in Crandell-Rees Feline Kidney (CRFK) cells, while DENV GFP titer was measured by plaque assay in VERO cells.

The monocytic cell line THP-1 derived from an acute monocytic leukemia patient was used in HIV-1 GFP infection assays, while the hepatocyte-derived carcinoma cell line Huh7 was used with DENV GFP . Cell viability was evaluated using the Cell-Titer GLO assay kit (Promega, Madison, WI, USA) as previously described [31] . Briefly, 7.5 × 10 3 Huh7 cells/well or 4 × 10 4 THP-1 cells/well were seeded in 96-well dark plates and treated with isolated AAs dissolved in methanol at concentrations ranging from 0.05 to 200 µM for 72 h. Afterwards, Cell-Titer GLO reagent was added in each well of room temperatureequilibrated plates. Luminescence was measured 10 min after shaking the plates for 2 min using a microplate spectrophotometer (Synergy H1, Biotek, Québec, QC, Canada). The percentage of viable cells was calculated at each concentration relatively to cells treated with matched concentrations of MetOH.

To quantify HIV-1 GFP and DENV GFP replication inhibition, 4.0 × 10 4 THP-1 and 7.5 × 10 3 Huh7 seeded cells per well were treated with indicated concentrations of alkaloids (from 0.05 to 200 µM) and then infected with HIV GFP at MOI = 0.1 or DENV GFP at MOI = 0.02. After 72 h, the percentage of infected cells was measured using a FC500 MPL cytometer (Beckman Coulter, Inc., Brea, CA, USA) and analyzed with the FlowJo software (BD, FlowJo LLC, Ashland, OR, USA). Matched concentrations of methanol were used as a negative control. Raltegravir was used as a positive control of HIV-1 inhibition, while lycorine was the positive control of DENV inhibition. All experiments were performed three times. CC 50 and EC 50 values were calculated using QuestGraph IC50 (MLA, Quest Graph™ IC50 Calculator". AAT Bioquest, Inc., Sunnyvale, CA, USA, https://www.aatbio.com/tools/ic50-calculator (accessed on 18 December 2021)).

HaCaT, spontaneously immortalized keratinocytes from adult skin, and AGS gastric adenocarcinoma cells were purchased from Service Cell Line (GmBH, Eppelheim, CLS, Germany). A431 (ATCC-CRL1555) human epidermoid carcinoma cells were from American Type Culture Collection (ATCC, Manassas, VA, USA). All mentioned cell lines were cultured in Dulbecco's Modified Eagle's Medium (DMEM, Sigma Chemical Co., St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone Laboratories, Inc. Logan, UT, USA) at 37 • C in a humidified atmosphere of 5% CO 2 . Absence of mycoplasma contamination was verified for all cell lines. The purified alkaloids were resuspended in DMSO and added to the cell medium at concentrations varying from 0.5 to 50 µM as indicated.

Cell viability was evaluated by measuring the reduction of 3-(4,5-dimethylthiazol-2) 2,5-diphenyltetrazolium bromide (MTT) to formazan by the mitochondrial enzyme succinate dehydrogenase. Briefly, 10 × 10 3 cells were seeded on 96-well plates and exposed to different concentrations of total extract or metabolites for 48 h. and 72 h. MTT/PBS solution (0.5 mg/mL) was then added to the wells and incubated for 3 h at 37 • C in a humidified atmosphere. The reaction was stopped by removal of the supernatant followed by dissolving the formazan product in acidic isopropanol and the optical density was measured with Synergy H4 microplate reader Gen5 2.07 (Thermofisher, Waltham, MA, USA) using a 570 nm filter. Under these experimental conditions, no undissolved formazan crystals were observed. Cell viability was assessed by comparing the optical density of the treated samples compared to the controls. Statistical analyses were carried out using the GraphPad Prism version 8.1.2 (https://www.graphpad.com/scientific-software/ prism/ (accessed on 18 December 2021)). Data were represented as the mean standard deviation and analyzed for statistical significance using ordinary one-way analysis of variance (ANOVA) and multiple comparisons. For all tests, p < 0.05 was considered to indicate a statistically significant difference. 

2:1) obtaining an amorphous solid identified as 11-hydroxyvittatine (5, 13.4 mg). The residue (31.2 mg) of fraction F6.4 was purified by TLC eluted with EtOAc-MeOH-H 2 O (7:2:1) obtaining an amorphous solid identified as pancracine

ESIMS (+) m/z: 288

ESIMS (+) m/z: 332

CD 3 OD) data are in agreement to those previously reported

ESIMS (+) m/z: 316

CDCl 3 ) data are in agreement to those previously reported

ESIMS (+) m/z: 449

ESIMS (+) m/z: 272

CD 3 OD) data are in agreement to those previously reported

ESIMS (+) m/z: 302

CDCl 3 ) data are in agreement to those previously reported

CD 3 OD) data are in agreement to those previously reported

ESIMS (+) m/z: 302 [M + H] + . 11-Hydroxyvittatine

CD 3 OD) data are in agreement to those previously reported

ESIMS (+) m/z: 288

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Alkaloide aus Hippeastrum aulicum var. robustum

Alkaloids of the Amaryllidaceae. VIII. The Structures of Narcissamine, Pseudolycorine and Methylpseudolycorine

Isolation of pretazettine from Narcissus tazetta L

Synthesis of the alkaloid natural products (+)-plicane and (−)-obliquine, using polymer-supported reagents and scavengers

13 C nuclear magnetic resonance spectra of amaryllidaceae alkaloids. I alkaloids with the crinane skeleton

Alkaloids from Narcissus cantabricus

Four new crinine-type alkaloids from Sternbergia species

Crinum alkaloids: Their chemistry and biology

Studies on the alkaloids of Amaryllidaceae. 9. 6-Alpha-Beta-Hydroxy-3-0-Mehylepimaritidine, 2 new alkaloids from Narcissus tazetta L. var chinensis roem

Alkaloids from Leucojum vernum

Narcissus alkaloids, III. 9-O-demethylhomolycorine from Narcissus confusus

9-O-Demethylhomolycornine from Egyptian Narcissus tazetta

Identification of 11-hydroxyvittatine in Sternbergia lutea

Phytochemical investigation of Hippeastrum vittatum

Alkaloids analysis of Habranthus cardenasianus (Amaryllidaceae), anti-cholinesterase activity and biomass production by propagation strategies

6-Hydroxycrinamine and haemanthidine

Alkaloids from Hippeastrum equestre Herb.-5. Circular dichroism studies

Recent advances in biomimetic natural product synthesis

The biological activity of alkaloids from the amaryllidaceae: From cholinesterases inhibition to anticancer activity

and More Basic NMR Experiments: A Practical Course

Alkaloids from Crinum amabile

CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia

High-throughput Screening Using Dengue Virus Reporter Genomes

Institutional Review Board Statement: Not applicable.

Data Availability Statement: Not applicable.

The authors declare no conflict of interest.