key: cord-0920358-1hlxxlx9
authors: Lv, Huibin; So, Ray T. Y.; Yuan, Meng; Liu, Hejun; Lee, Chang-Chun D.; Yip, Garrick K.; Ng, Wilson W.; Wilson, Ian A.; Peiris, Malik; Wu, Nicholas C.; Mok, Chris K. P.
title: Evidence of antigenic imprinting in sequential Sarbecovirus immunization
date: 2020-10-15
journal: bioRxiv
DOI: 10.1101/2020.10.14.339465
sha: bfb3643a22125fba2f151f30f0fc5177aa0c943c
doc_id: 920358
cord_uid: 1hlxxlx9

Antigenic imprinting, which describes the bias of antibody response due to previous immune history, can influence vaccine effectiveness and has been reported in different viruses. Give that COVID-19 vaccine development is currently a major focus of the world, there is a lack of understanding of how background immunity influence antibody response to SARS-CoV-2. This study provides evidence for antigenic imprinting in Sarbecovirus, which is the subgenus that SARS-CoV-2 belongs to. Specifically, we sequentially immunized mice with two antigenically distinct Sarbecovirus strains, namely SARS-CoV and SARS-CoV-2. We found that the neutralizing antibodies triggered by the sequentially immunization are dominantly against the one that is used for priming. Given that the impact of the background immunity on COVID-19 is still unclear, our results will provide important insights into the pathogenesis of this disease as well as COVID-19 vaccination strategy.

Antigenic imprinting, which describes the bias of antibody response due to previous 28 immune history, can influence vaccine effectiveness and has been reported in different 29 viruses. Give that COVID-19 vaccine development is currently a major focus of the world, 30 there is a lack of understanding of how background immunity influence antibody 31 response to SARS-CoV-2. This study provides evidence for antigenic imprinting in 32 Sarbecovirus, which is the subgenus that SARS-CoV-2 belongs to. Specifically, we 33 sequentially immunized mice with two antigenically distinct Sarbecovirus strains, namely 34 SARS-CoV and SARS-CoV-2. We found that the neutralizing antibodies triggered by the 35 sequentially immunization are dominantly against the one that is used for priming. Given 

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