key: cord-0917862-5i19w7oh authors: Hazan, Sabine title: Pre-Existing Microbiome Signature in a SARS-CoV-2 Discordant Family date: 2022-02-07 journal: Case Rep Gastroenterol DOI: 10.1159/000520625 sha: f8fe75c7c2e65f276f20cc546f06a339514a29fa doc_id: 917862 cord_uid: 5i19w7oh Our index patient is a 19-year-old man with Crohn's disease. After developing symptoms consistent with COVID-19, he, his 62-year-old father, and 14-year-old sister tested positive for SARS-CoV-2 in May 2020. Despite a shared household, his 50-year-old mother with a history of asthma and his healthy brother and sister-in-law (a married couple) remained negative. The index patient and his mother had undergone microbiome analysis in May 2019, following his brother and his sister-in-law in November 2020. We observed significant differences between the fecal microbiota of the SARS-CoV-2-positive son and those of his healthy family. There were differences in the bacterial phylum, class, order, family, and genus level with the increased relative abundance of Bacteroidetes and reductions or deletions in bacterial diversity, particularly of the Bifidobacterium family. This unique study may signal a new exploratory avenue for the prevention or treatment of SARS-CoV-2 infections. Little is known of the molecular mechanisms or genetic predispositions that may underpin the hyperinflammatory response during or after acute infection [1] . Mounting scientific evidence confirms that infections, including SARS-CoV-2, can provoke a significant loss of microbial diversity in the gut and that this loss can suppress the immunologic response [2, 3] . Two important microbiota commensals, Bifidobacteria and Lactobacillus [4] , are significantly reduced in COVID-19 patients [5] . SARS-CoV-2 may also alter cytokine levels that increase the severity of COVID-19. Enhancing gastrointestinal microbiome diversity might be helpful for lowering SARS-CoV-2 infection risk or COVID-19 severity [6, 7] . The household included the 19-year-old index patient who had Crohn's disease, his 50-year-old mother with a history of asthma and no medication prior to her visit, and his 28-year-old brother, 26-year-old sister-in-law, 62-year-old father, and 14-year-old sister. The mother and son visited the Clinic in May 2019 to consult regarding the son's Crohn's disease. At the time, he was using mesalamine delayed release 1.2 g tablets. Following the consultation in May 2019, the patient, and his mother both underwent microbiome analysis. In May 2020, the index patient developed fever (102°C), sore throat, rhinorrhea, productive cough, and diarrhea, but no dyspnea. He tested positive for SARS-CoV-2 by PCR. His mesalamine delayed release was discontinued, and he was treated with 5 days of hydroxychloroquine 200 mg po BID, vitamin C 3,000 mg po qd, and zinc 50 mg po qd and 10 days of vitamin D 3,000 IU po qd. The patient's 62-year-old father and 14-year-old sister tested positive for SARS-CoV-2 by PCR within 2 days of the son's positive test. There was no sample collected at the sickness time due to the SARS-CoV-2 safety concerns to collect feces by both the father and daughter. The mother was the sole caregiver for the sick family members and shared a bed and bathroom with her ill husband. The mother's PCR collected on the same day as those for the rest of the family was negative. At the same period, he infected the remainder of her family with SARS-CoV-2. The healthy brother and sister-in-law had no underlying conditions, were on no medications, and tested negative for SARS-CoV-2 on the same day as all family members were tested. The family has never used any pro-or prebiotics, they shared the same diet, and all had body mass indices <26. The mother, brother, and sister-in-law have remained well since May 2020. We conducted a microbiome analysis on a stool from four family members. We compared interindividual differences between the index patient and his mother, brother, and sister-inlaw in phylum, class, order, family, and genus. There was no sample collected for father and daughter at the sickness time due to the SARS-CoV-2 safety concerns for feces collections by them. We have a continuous validation system for microbiome analysis. Therefore, all microbiome analysis assays through the clinical study were reproducible through the study period. Additionally, there were not any changes in the family diet and medicine through the period. We obtained written informed consent from all subjects before initiating the study procedure. DNA was extracted from the stools of the index patient and mother in May 2019 and for the brother and sister-in-law in November 2020. DNA was then quantitated and normalized for downstream library fabrication utilizing Hazan-Papoutsis shotgun methodology [8] . Prepared and indexed libraries were subsequently pooled and sequenced on the Illumina NextSeq 550 System. Sample FASTQ files were analyzed with a computational tool profiling the microbial communities from metagenomic sequencing data with a species-level resolution. Finally, we compared the microbiome of the SARS-CoV-2-positive index patient Hazan: Case Study of Microbiome in SARS-CoV-2 www.karger.com/crg with those of his SARS-CoV-2-negative mother, brother, and sister-in-law in the same household, using OneCodex Software using both quick compare, custom plot, and OneCodex Jupyter notebook using Python for Shannon and Simpson Index, Alpha and beta diversity, and Chao analysis. In total, four stool samples were sequenced for this study. We compared gut microbiota composition and relative abundance in the stool samples of the SARS-CoV-2-positive patient and his SARS-CoV-2-negative family members to assess whether their gut microbiota composition differed. We hypothesized that the patient and well household members might have different microbiota signatures at the family and genus levels. Figure 1 depicts the relative abundance at the genus level, including Bifidobacterium genus in the SARS-CoV-2-positive (+) index patient and the SARS-CoV-2-negative (−) mother, brother, and sister-in-law by the month of collection. The initial difference between the microbiome of mother and son prior the COVID-19 period is possibly due to the mother's Crohn's disease involvement. Figure 2 and Table 1 show relative abundance reductions and expansions at the phylum level and the same for class, order, family, and genus for the microbiome of the SARS-CoV-2-positive son compared to those of his SARS-CoV-2-negative mother, brother, and sister-in-law. Reductions were observed at the phylum level for Actinobacteria, class level for Actinobacteria, order level for Bifidobacteriales, family level for Bifidobacteriaceae and Ruminococcaceae, and at the genus level for Bifidobacterium. Expansions were observed in the microbiome of the SARS-CoV-2-positive index patient compared to his negative household members at the phylum level for Bacteroidetes, class level for Bacteroidia, order level for Bacteroidales, and family level for Bacteroidaceae, and genus level for Bacteroides. We calculated alpha diversity based on the gene profiles using the Shannon and Simpson index. The Simpson microbial diversity (alpha diversity) was significantly decreased in SARS-CoV-2-positive patient compared to his negative mother, brother, and sister-in-law accordingly (0.66, 0.96, 0.96 and, 0.85 p < 0.00001 for Simpson diversity). The Shannon diversity index was (2.94, 5.52, 5.53, 3.65) accordingly. We are unaware of any other microbiome analyses for SARS-CoV-2-positive and negative family members for which studies in both a positive and negative family member preceded the COVID-19 pandemic. The mother was the sole caregiver for all the sick family members and shared a bed and bathroom with her SARS-CoV-2-positive husband. That the mother, brother, and sister-in-law were PCR-negative and remained asymptomatic despite living with three positive family members suggests that considerable exposure, and second, that some of the family members were less susceptible to infection than others. The composition and relative abundance of various phyla, classes, orders, families, and genera in the gut microbiota of the family members who remained well may have played a role in modulating their immune responses. Gut microbiota influence and modulate host immune responses [9] . For example, crosstalk between the liver and the intestine is essential for natural immune surveillance, adaptive immune response regulation, and nutrient metabolism, and elimination of toxic bacterial metabolites [10] . Additionally, in HIV patients, the altered gut microbiome increases disease susceptibility [11] . In our case, a year before he became infected, the SARS-CoV-2-positive index patient had significantly less beneficial Bifidobacterium commensals and significantly more Bacteroides than his uninfected mother. He also had fewer beneficial and more Bacteroides than his uninfected brother and sister-in-law, who volunteered to be tested later during the COVID-19 pandemic. Several authors have hypothesized that the microbiome might influence susceptibility to SARS-CoV-2 infection or COVID-19 severity [12] [13] [14] [15] , and Ahlawat has described immunological coordination between the gut and lung in SARS-CoV-2 infections [16] . A few groups have assessed the microbiomes of patients hospitalized for COVID-19. Zuo et al. [17] observed a fungal dysbiosis in patients hospitalized for COVID-19, with increases in Candida albicans, Candida aureus, and Aspergillus flavus; his group observed a similar dysbiosis in the microbiome of patients with community-acquired pneumonia but not in those of healthy controls. Zuo et al. [17] And Yeoh et al. [18] described the microbiomes of medicated and nonmedicated COVID-19 patients collected during hospitalization as significantly different from those of uninfected individuals (p < 0.01). Gut commensals with known immunomodulatory potential such as Faecalibacterium prausnitzii, Eubacterium rectale, and Bifidobacteria were underrepresented in the microbiome COVID-19 patients compared to the microbiome of uninfected individual [18] . An aggressive inflammatory response is a major pathophysiological characteristic of SARS-CoV-2 infections [18] . Yeoh's findings of reductions in relative abundance of Faecalibacterium and Eubacterium rectale are of interest; both species may use dietary fiber to increase butyrate ratio/levels [19] which, in turn, can down-regulate inflammation [20] . However, we saw relative abundance reductions in neither of these species. Like Yeoh et al. [18] , who compared hospitalized COVID-19 patients and controls, we observed a significantly lower relative abundance of important Bifidobacterium commensals in the son's microbiome compared to those of his mother, brother, and sister-in-law. However, we also observed expansions in the relative abundance of Bacteroidetes. Setoyama et al. [21] has suggested that the severity of gut inflammation in ulcerative colitis may be linked to Bacteroides vulgatus, and that this inflammation might be repressed by Bifidobacterium. Bifidobacteria are Gram-positive, anaerobic, saccharolytic Actinobacteria that fermentatively degrade complex carbohydrates and produce acetate and lactate, which are, in turn, used by butyrateand propionate producing colon bacteria [22] . These two primary short-chain fatty acidsbutyrate and propionate -have significant anti-inflammatory effects. Bifidobacteria are particularly abundant in infants and decline steadily with age. Breastfed infants have higher levels of bifidobacterial than formula-fed infants, and the levels decrease after breastfeeding ends. Various Bifidobacteria spp. have been shown to induce dendritic cell maturation, T-cell polarization (toward a Th1 response), production of the modulatory cytokine IL10, and decreases in TNFa and INFg production, and subcellular fractions of Bifidobacteria have been identified as effectors of CD8C T-cell activation [23] . We hypothesize that microbiome alterations at the phylum level may have increased the son's susceptibility to SARS-CoV-2 compared to his household members, who were negative and remained asymptomatic, mainly because there is increasing evidence of gut microbiota dysbiosis in COVID-19 patients despite respiratory etiology of the disease [24] [25] [26] . If this were confirmed in additional studies, a baseline microbiome analysis might predict SARS-CoV-2 susceptibility and buy time for dietary interventions with pre-or probiotics to improve resistance. This hypothesis is supported by Kim et al. [27] , who recently stated that an altered and leaky gut microbiota might allow SARS-CoV-2 into the circulatory system and result in multiorgan complications. Limitations of our data are as follows. We report on a single household, and only one of the three persons who became ill had a prior microbiome analysis. Although everyone was tested at one point in time and three of the household members -the index patient's mother, brother, and sister-in-law -remained healthy, any of the three might have developed asymptomatic SARS-CoV-2 infections later. It would have been desirable to repeat the microbiome analysis for the son after his illness and note any post-infection alterations and perform antibody tests in the PCR-negative asymptomatic family members to look for evidence of infection. We suspect that a baseline microbiome analysis might predict SARS-CoV-2 susceptibility and that a "susceptible" signature might be remediable. 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