key: cord-0913923-vyfmzyb5
authors: Romero-Olmedo, Addi J; Schulz, Axel Ronald; Hochstätter, Svenja; Gupta, Dennis Das; Hirseland, Heike; Staudenraus, Daniel; Camara, Bärbel; Volland, Kirsten; Hefter, Véronique; Sapre, Siddhesh; Krähling, Verena; Müller-Kräuter, Helena; Chung, Ho-Ryun; Mei, Henrik E; Keller, Christian; Lohoff, Michael
title: Dynamics of humoral and T-cell immunity after three BNT162b2 vaccinations in adults older than 80 years
date: 2022-04-06
journal: Lancet Infect Dis
DOI: 10.1016/s1473-3099(22)00219-5
sha: 135e7fb8f74c8d7ba59546f6cd5ec9659a1cf11c
doc_id: 913923
cord_uid: vyfmzyb5

nan

1 against the omicron variant. 8 As for our cohort, our data show two important aspects of a third compared with a second dose-namely, peak virus-specific T-cell frequencies were not further increased by a third dose, and average per-cell production of IFNγ remained unaltered and was still remarkably lower than in recovered donors of a similar age. Thus, at least in older adults, the durability and quality of vaccine-induced immunity should Dynamics of humoral and T-cell immunity after three BNT162b2 vaccinations in adults older than 80 years A third mRNA-based booster vaccination is the currently favoured strategy to maintain protection against SARS-CoV-2 infection. Yet, significant waning of specific immunity within 6 months after two doses, 1 along with a higher incidence of breakthrough infections associated with the time elapsed since the second dose, 2,3 raise concerns regarding the durability of immunity also after the booster vaccination.

We compared the specific humoral and cellular responses (figure; appendix pp 10-13) after three versus two BNT162b2 (Pfizer-BioNTech) doses in a cohort of adults older than 80 years (median age 83 years [IQR 81-86]; appendix pp 3-4) at risk for severe COVID-19 and immune senescence. Our data demonstrate the induction of marginally higher spike S1-specific blood IgG concentrations 2 weeks after three than after two doses (appendix p 5). By contrast, functionally relevant receptor binding domain-specific IgG (figure A) and SARS-CoV-2-neutralising antibody (appendix p 5) titres were substantially increased after three compared with two doses, reflecting enhanced antibody production or affinity maturation.

By contrast, spike-specific CD4 T-cell frequencies reached similar levels after two and three doses (figure B; appendix p 5). After the respective acute response, frequencies returned to approximately pre-third vaccination levels, with no significant differences in the rate of decline after the second and third vaccinations (figure B; appendix p 6). Quantified cytoplasmic expression of the effector cytokine interferon γ (IFNγ) indicated functional enhancement of spike-specific T cells upon second but not further upon third vaccination, while more cytoplasmic IFNγ was found in spike-specific CD4 T cells from adults older than 80 years who had recovered from COVID-19 (appendix p 5). Thus, even a third BNT162b2 dose failed to induce durably enhanced quantities of spike-specific T cells and a functional quality reached after natural infection. Neither age nor comorbidities were significantly correlated with the observed immune response, perhaps due to the limited size of our cohort (appendix pp 7-9).

Concentrations of S1-specific IgG and neutralising antibodies also declined from the acute responses at weeks 5 and weeks 26, but at a lower rate and with an extended half-life after the third (week 40) compared with the second (week 24) dose (figure A; appendix pp 5-6), yielding more persistent, enhanced IgG quantity or quality after the third than after the second vaccination.

We conclude that a third dose of BNT162b2 in older adults, while establishing immunity in primary non-responders, 4 induces a durably escalated humoral response in the bulk of vaccinees for at least 3 months, indicating longer lasting humoral immunity. In a younger cohort, this boost also led to a strong increase of neutralising antibodies against the omicron (B.1.1.529) variant and protection from infection with the omicron variant. 5, 6 Although neutralising antibody data for omicron are not yet available for our cohort, the strong rise in titres of neutralising antibodies against the BavPat1/2020 isolate used in our neutralisation assay (appendix p 5) suggests better neutralisation against omicron by the booster dose than for the second dose, as also demonstrated by others, 7 at least in the short term. The level of T-cell immunity to SARS-CoV-2 in peripheral blood required for protection is still not established, although peripheral T cells induced by BNT162b2 apparently react well 

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