key: cord-0912075-p0gyyl3c authors: Hsu, Albert L.; Guan, Minhui; Johannesen, Eric; Stephens, Amanda J.; Khaleel, Nabila; Kagan, Nikki; Tuhlei, Breanna C.; Wan, Xiu‐Feng title: Placental SARS‐CoV‐2 in a Pregnant Woman with Mild COVID‐19 Disease date: 2020-08-04 journal: J Med Virol DOI: 10.1002/jmv.26386 sha: 5b7637efa87df575d2a61a9225b3f0b78e04d720 doc_id: 912075 cord_uid: p0gyyl3c BACKGROUND: The full impact of COVID‐19 on pregnancy remains uncharacterized. Current literature suggests minimal maternal, fetal, and neonatal morbidity and mortality.(1) COVID‐19 manifestations appear similar between pregnant and non‐pregnant women.(2) OBJECTIVES/STUDY DESIGN: We present a case of placental SARS‐CoV‐2 virus in a woman with mild COVID‐19 disease, then review the literature. RT‐PCR was performed to detect SARS‐CoV‐2. Immunohistochemistry staining was performed with specific monoclonal antibodies to detect SARS‐CoV‐2 antigen or to identify trophoblasts. RESULTS: A 29 year‐old multigravida presented at 40‐4/7 weeks for labor induction. With myalgias two days prior, she tested positive for SARS‐CoV‐2. We demonstrate maternal vascular malperfusion, with no fetal vascular malperfusion, as well as SARS‐CoV‐2 virus in chorionic villi endothelial cells, and also rarely in trophoblasts. CONCLUSIONS: To our knowledge, this is the first report of placental SARS‐CoV‐2 despite mild COVID‐19 disease (no symptoms of COVID‐19 aside from myalgias); patient had no fever, cough, or shortness of breath, but only myalgias and sick contacts. Despite her mild COVID‐19 disease in pregnancy, we demonstrate placental vasculopathy and presence of SARS‐CoV‐2 virus across the placenta. Evidence of placental COVID‐19 raises concern for placental vasculopathy (potentially leading to fetal growth restriction and other pregnancy complications) and possible vertical transmission – especially for pregnant women who may be exposed to COVID‐19 in early pregnancy. This raises important questions of whether future pregnancy guidance should include stricter pandemic precautions, such as screening for a wider array of COVID‐19 symptoms, increased antenatal surveillance, and possibly routine COVID‐19 testing throughout pregnancy. This article is protected by copyright. All rights reserved. This raises important questions of whether future pregnancy guidance should include stricter pandemic precautions, such as screening for a wider array of COVID-19 symptoms, increased antenatal surveillance, and possibly routine COVID-19 testing throughout pregnancy. As of 18 July 2020, the coronavirus disease 2019 (COVID-19) pandemic has resulted in 3,630,587 cases and 138,782 deaths in the United States. 1 The full impact of COVID-19 on pregnancy remains to be fully characterized, especially as women who were exposed to COVID-19 early in their first trimester have yet to reach full term, as of the time of writing this manuscript. The current literature suggests minimal maternal, fetal, and neonatal morbidity and mortality for women with COVID-19 in pregnancy. 2 This article is protected by copyright. All rights reserved. women, 3 until a recent publication from the US CDC Morbidity and Mortality Weekly Report (MMWR). 4 Before this study, we searched PubMed and Medline on 28 May 2020 (updated on 28 June 2020) for articles describing the impact of COVID-19 in pregnancy, using the search terms "COVID-19" or "SARS-CoV-2" and "pregnancy" or "placenta" with no time or language restrictions. We found only previously published research that describes finding SARS-CoV-2 virus in the placentas of women with moderate-to-severe COVID-19 disease. We found no published work about SARS-CoV-2 virus in the placentas of women with mild COVID-19 disease. Two reports suggest that pregnant women with COVID-19 may be more likely to be admitted to the ICU and more likely to be intubated, but all reports consistently state that severe COVID-19 disease during pregnancy is uncommon. We found six manuscripts which describe SARS-CoV-2 virus in the placentas of women with moderate-to-severe COVID-19 disease. We found no published work about SARS-CoV-2 virus immunohistochemistry in the placentas of women with mild COVID-19 disease. In this case study, we present a case of placental SARS-CoV-2 virus in a woman with an uncomplicated pregnancy and mild COVID-19 disease. Here, we review the literature on COVID-19 disease and pregnancy, and subsequently discuss the key messages of this case. This article is protected by copyright. All rights reserved. A pregnant woman was evaluated at University of Missouri Women and Children's Hospital. Institutional review board approval was obtained; information was obtained from medical records. Reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to detect SARS-CoV-2. A gynecological pathologist examined the placenta and performed histolopathology. Sections were formalin-fixed and paraffin-embedded; slides were cut and subjected to hematoxylin-and-eosin or immunohistochemistry (IHC) staining. IHC was performed with SARS-CoV-2 nucelocapsid-specific or cytokeratin-7 specific monoclonal antibody to detect SARS-CoV-2 antigen or to identify trophoblasts, respectively. In April 2020, a 29 year-old multigravida presented at 40-4/7 weeks for labor induction. She had tested positive for SARS-CoV-2, with her only symptoms being myalgias two days prior. Her parents and siblings had been in self-isolation for COVID-19 positivity; her husband was asymptomatic and tested negative for COVID-19, but he had been exposed to a workplace (meatpacking facility) outbreak. Her prenatal course was uncomplicated, with no gestational hypertension or any other pregnancy complications. Her myalgias improved prior to admission. She was afebrile and asymptomatic with normal vital signs throughout hospitalization. Consistent with other reports of laboratory abnormalities associated with COVID-19, her inpatient labwork showed some mild elevations in her LFTs and C-reactive protein, but were otherwise unremarkable (Table 1) . This article is protected by copyright. All rights reserved. A liveborn male infant was delivered vaginally. Newborn course was uneventful; with routine resuscitation and newborn APGARs of 8 and 9 at 1 and 5 minutes; points were taken off for color only. Birthweight was 3521g (66 th percentile), length was 48 cm (14 th percentile) and head circumference was 35 cm (64 th percentile), all appropriate for gestational age. His physical was unremarkable, with a small skin tag on his right cheek and dermal melanocytosis on buttock, appropriate for ethnicity. He passed hearing screen and screen for critical congenital heart defects (normal-preductal 99%, postductal 99%). He was discharged home at 36 hours of life, with a plan for close follow-up from his pediatrician. COVID-19 reverse transcriptase-polymerase chain reaction (RT-PCR) test was negative at 24 hours. At one-week follow-up, newborn was breastfeeding well, with no fevers or respiratory distress. Two months later, mother and baby are doing well. A gynecological pathologist examined the placenta and performed histolopathology. Sections were formalin-fixed and paraffin-embedded; slides were then cut and subjected to hematoxylin-and-eosin or immunohistochemistry (IHC) staining. Placenta had a marginally-inserted three-vessel umbilical cord; placental membranes were thin and transparent. Placental weight was 538 grams, at the 60 th percentile for gestational age. No gross lesions were seen on fetal or maternal placental surfaces; parenchymal sections were unremarkable. On histology, placental membranes showed decidua with scattered arterioles with thickened smooth muscle, consistent with hypertrophic arteriolopathy ( Figure 1a ) and subchorionic laminar necrosis (1b). Placental disc showed focal lympho-histiocytic inflammation consistent with chronic villitis (1c) and scattered islands of extravillous trophoblasts (1d). No evidence of fetal thrombi (villous sclerosis or villous karyorrhexis) were seen. Overall histology is consistent with acute This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved. system in New York State 7 and found that pregnant women were not at an increased risk for ICU admission compared with nonpregnant women (p=0.22); 7 in this study, 15 .1% of nonpregnant females were admitted to the ICU compared to 9.8% of pregnant patients. 7 positive cases). 16 Fortunately, none of these studies reported any maternal deaths. Two other studies, 4, 17 suggest that pregnant women with COVID-19 have a higher risk of severe disease than nonpregnant women. A Swedish study showed pregnant Swedish women were 5 times more likely to be admitted to the ICU and 4 times more likely to receive mechanical ventilation than were nonpregnant women. 17 Also, a US CDC report reviewed 326,335 women of reproductive age (15- pregnant women in this study had an even lower overall risk of ICU admission than the previous 12 case series. [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] All of these studies agree upon a low level of maternal mortality (16 deaths, or 0.2% according to MMWR) from COVID-19. [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] While this is reassuring overall, more data is needed, especially regarding pregnancies with exposure to COVID-19 in the first trimester. There are also several reports suggesting a potential low risk of in utero vertical transmission from mother to fetus. In an Italian series of 22 women affected by COVID- were all positive for SARS-CoV-2 by PCR testing. 19 symptoms. 20 Baud et al describe a second-trimester miscarriage in which the mother (nasopharyngeal swab) and placental submembranes and placental cotyledons were positive for SARS-CoV-2 on RT-PCR. 21 Hosier et al describe SARS-CoV-2 localization to syncytiotrophoblast cells at the maternal-fetal interface of the placenta, with no evidence of vasculopathy. 22 Chen et al describe 9 patients who had a caesarean section, with amniotic fluid, cord blood, neonatal throat swab, and breastmilk samples from six patients all testing negative for SARS-CoV-2. 23 This article is protected by copyright. All rights reserved. Ferriaolo et al describe an asymptomatic woman with a positive nasopharyngeal swab for COVID-19 in Italy, who had a Caesarean section and positive placental swabs for SARS-CoV-2 RNA. 24 Dong et al report a case of SARS-CoV-2 IgM in a neonate born to a COVID-19 positive mother. 25 Collectively, this literature suggests that there may be a low risk of vertical transmission of COVID-19 from mother to fetus in utero. In our report, SARS-CoV-2 was found throughout the placenta on immunohistochemistry, and newborn was COVID-19 negative. As this patient was exposed to COVID-19 in her third trimester of pregnancy, it remains unclear whether women who are exposed to COVID-19 earlier in pregnancy (or multiple times during malperfusion in 3 of 20 placentas. 27 In a study at Northwestern University, Shanes et al found that the most common findings in COVID-positive placentas were decidual arteriopathy and intervillous thrombi. 28 However, it is notable that signs of fetal and maternal malperfusion are non-specific and can be seen in other conditions, such as hypercoagulable states, like lupus anticoagulant and protein C or S deficiency, gestational hypertension, 29 pre-eclampsia, as well as in patients with no specific medical history. The inexorable rise in COVID-19 cases in the United States amid decreasing adherence to public health recommendations raises concern for the possibility of viral mutations. In light of the CDC's MMWR report, 4 the US CDC also issued updated (6/25/20) recommendations 30 regarding pregnancy and COVID-19, encouraging pregnant women to "limit interactions with other people as much as possible," to "take precautions to prevent getting COVID-19 when you do interact with others," and to be aware that "some babies have tested positive for the virus shortly after birth. It is unknown if these babies got the virus before, during, or after birth." While two reports suggest that pregnant women with COVID-19 may be more likely to be admitted to the ICU and more likely to be intubated than non-pregnant women, 4, 17 all reports consistently state that severe COVID-19 disease during pregnancy is uncommon. [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] We found six manuscripts which describe SARS-CoV-2 virus in the placentas of women with moderate-to-severe COVID-19 disease. To date, there is still no other published work about SARS-CoV-2 virus by immunohistochemistry in the placentas of women with mild COVID-19 disease. This article is protected by copyright. All rights reserved. To our knowledge, this is the first report of placental COVID-19 despite mild COVID-19 disease in pregnancy (with no symptoms of COVID-19 aside from myalgias); specifically, this patient had no fever, cough, or shortness of breath, but only myalgias and sick contacts. Evidence of placental COVID-19 raises concern for possible placental vasculopathy (potentially leading to fetal growth restriction, pre-eclampsia, and other pregnancy complications) as well as for potential vertical transmission -especially for pregnant women who may be exposed to COVID-19 in early pregnancy. Despite her having mild COVID-19 disease in pregnancy, we demonstrate placental vasculopathy and presence of SARS-CoV-2 virus across the placenta. While our case shows features of maternal vascular malperfusion (extra-villous trophoblastic lesions and subchorionic laminar necrosis), no evidence of fetal vascular malperfusion were seen. As this patient was exposed to COVID-19 in her third trimester of pregnancy, it remains unclear whether women who are exposed to COVID-19 earlier in pregnancy (or multiple times during pregnancy) may have a greater risk of fetal vascular malperfusion, which may result in pre-eclampsia, fetal growth restriction, or other obstetric complications. Further studies are needed, especially from pregnancies with exposure to COVID-19 in the first trimester. This report also raises important concerns regarding the possibility of birth defects, miscarriages, and stillbirths with COVID-19 infection, as well as the question about potential protective or detrimental effects of breastfeeding. Data responsive to these critical issues remain limited and preliminary. Of note, women who were exposed to COVID-19 in their first trimester in the United States still have not had any full-term This article is protected by copyright. All rights reserved. deliveries, and remain in early stages of their pregnancies; thus, we are not able to comment on the possibilities of birth defects with COVID-19 at the current time. Regarding miscarriage, one early meta-analysis 2 noted 4 cases of miscarriage out of 324 pregnant women with COVID-19; most related studies similarly lack a wellcharacterized control group for appropriate comparative analyses. There are also isolated case reports suggesting that late miscarriage may be a presenting manifestation of COVID-19. 21, 31 Finally, an early report from the United Kingdom 32 demonstrated a concerning increase in stillbirth rates during the COVID-19 pandemic; specifically, those authors report "3 stillbirths among 247 completed pregnancies" in women with confirmed COVID-19, for a rate of 6.98 vs 1.19 per 1000 births (P=.01), with multifactorial causes likely. Regarding breastfeeding, early data suggest that transmission of COVID-19 through breastmilk is possible but rare. Cumulative data from six separate studies 23, 25, [33] [34] [35] [36] found that out of 20 mothers with COVID-19, only two had their breastmilk test positive with PCR. Of those two cases, one breastfed newborn tested positive for SARS-CoV-2 by PCR, and the other newborn tested negative for SARS-CoV-2; it is unclear whether that newborn breastfed or received expressed breastmilk. Ferrazi et al 35 reported that, of two women with COVID-19 who breastfed without a mask, both had newborns who tested positive for COVID-19 postpartum. In contrast, eight other COVID-19 positive mothers in this study breastfed while mask-wearing, and those eight newborns did not contract COVID-19; there was no mention of whether or not the breastmilk was tested for SARS-CoV-2. 35 This suggests that close contact associated with breastfeeding may increase the risk of transmission of infected respiratory secretions, and that mask-wearing may decrease this risk. Questions about birth defects, miscarriage, stillbirth, and This article is protected by copyright. All rights reserved. breastfeeding with COVID-19 remain critical areas for future research, but at the current time, data is limited, preliminary, and continually-evolving. To our knowledge, this is the first report of placental COVID-19 despite mild COVID-19 disease in pregnancy (with no symptoms of COVID-19 aside from myalgias). All other reports have shown placental COVID-19 in pregnancies with moderate-to-severe disease. This patient had no fever, cough, or shortness of breath, but only myalgias and sick contacts. In this report, SARS-CoV-2 was found in the placenta, but the newborn was COVID-19 negative. Our case shows maternal vascular malperfusion, with no features of fetal vascular malperfusion. Evidence of placental COVID-19 raises concern for placental vasculopathy and potential vertical transmission. Our report raises the question of whether future pregnancy guidance should include even stricter pandemic precautions, such as prenatal screening for a wider array of COVID-19 symptoms, increased antenatal surveillance recommendations, and possibly COVID-19 testing on a regular basis throughout pregnancy. 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Detection of SARS-CoV-2 in human breastmilk The Lancet Coronavirus disease 2019 among pregnant Chinese women: case series data on the safety of vaginal birth and breastfeeding BJOG Vaginal delivery in SARS-CoV-2-infected pregnant women in Northern Italy: a retrospective analysis BJOG This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved. Immunohistochemistry (IHC) staining of SARS-CoV-2 virus in a COVID-19 negative patient, delivery prior to the COVID-19 outbreak (Figure 2a) . IHC of SARS-CoV-2 from three placental sections (2b: under umbilical cord, 2c: central placental disc, 2d-e: peripheral placental disc at 20x and 40x). IHC of cytokeratin-7 (CK-7) marker in control ferret nasal turbinate tissue ( Figure 2f ). IHC of CK-7 from three placental sections (2g: under umbilical cord, 2h: central placental disc, 2i-j: peripheral placental disc at 10x and 20x). Arrows or brown staining indicate immunoreactive antigens. Bars = 20/50/100 µm shown at the right bottom corner of each panel. IHC was performed with SARS-CoV-2 nucleocapsid-specific rabbit monoclonal antibody (Sino Biological, Wayne, PA) and goat anti-rabbit IgG (Vector lab, Burlingame, CA). To identify trophoblasts, IHC was performed using rabbit recombinant anti-Cytokeratin 7 (CK7) monoclonal antibody (Abcam, Cambridge, MA) and goat anti-rabbit IgG (Vector lab, Burlingame, CA).