key: cord-0908788-s57a91i2 authors: Varanges, Vincent; Caglar, Baris; Lebaupin, Yann; Batt, Till; He, Weidong; Wang, Jing; Rossi, René M.; Richner, Gilles; Delaloye, Jean-Romain; Michaud, Véronique title: On the durability of surgical masks after simulated handling and wear date: 2022-03-23 journal: Sci Rep DOI: 10.1038/s41598-022-09068-1 sha: d5b7d60fb2d241c9705a40aaea1992a7b24a1dcb doc_id: 908788 cord_uid: s57a91i2 After the spread of COVID-19, surgical masks became highly recommended to the public. They tend to be handled and used multiple times, which may impact their performance. To evaluate this risk, surgical masks of Type IIR were submitted to four simulated treatments: folding, ageing with artificial saliva or sweat and washing cycles. The air permeability, mechanical integrity, electrostatic potential, and filtration efficiency (FE) of the masks were measured to quantify possible degradation. Overall, air permeability and mechanical integrity were not affected, except after washing, which slightly degraded the filtering layers. Electrostatic potential and FE showed a strong correlation, highlighting the role of electrostatic charges on small particle filtration. A slight decrease in FE for 100 nm particles was found, from 74.4% for the reference masks to 70.6% for the mask treated in saliva for 8 h. A strong effect was observed for washed masks, resulting in FE of 46.9% (± 9.5%), comparable to that of a control group with no electrostatic charges. A dry store and reuse strategy could thus be envisaged for the public if safety in terms of viral and bacterial charge is ensured, whereas washing strongly impacts FE and is not recommended. www.nature.com/scientificreports/ We thus propose to quantify the influence of handling and wearing on mask integrity and filtration efficiency, using laboratory-scale protocols which mimic extreme scenarios of masks' manipulation and wear, including repeated folding experiments, storage in artificial sweat and saliva and finally washing events. These worst-case scenarios are summarized in Table 1 and Fig. 1 provides micrographs of the mask structure with three layers and a visual summary of the performed tests. Air permeability. Figure 2a displays the permeability values as a function of input pressure for masks with the various treatments. The figure shows little scatter for all five different mask types and a linear dependency on the test pressure, with a correlation coefficient of 0.98 and 1. The air permeability values lie in the same range for all the treatments, except for the washed masks which show a slightly higher permeability overall. Data thus indicate that the severe folding and ageing treatments endured by the masks do not modify the air permeability of the fabric. (1) www.nature.com/scientificreports/ Table 2 reports the pressure drop induced by the masks according to EN14683. For all configurations, the average ΔP was between 41.4 and 46 Pa cm −2 , below the 60 Pa cm −2 stated in EN14683 with the lowest value in the case of the washed masks. Figure 2b displays the micrographs obtained after each treatment, whereas Fig. 3 displays SEM micrographs of a reference and washed mask. A clear impact of washing is observed on the middle layer whereas the inner and outer layers remain identical before and after washing. Figure 2c -e report the mechanical properties as a function of the layer and treatment. Young's moduli (as defined in the "Methods" section) of inner and outer layers (40.9 MPa and 35.8 MPa) are slightly higher than that of the middle layer (28.8 MPa), in the range reported by Ekabutr et al. 12 . This pattern repeats itself for the treated masks as observed in Fig. 2c and in Table 2 . The middle layer of the washed masks shows a slightly lower strain at break when compared to the other treatments (Fig. 2e) , whereas folding and ageing treatments do not significantly alter the mechanical properties of the three layers forming the mask structure. Electrostatic potential measurements. Surface potential results are displayed in Fig. 4 and Supplementary Fig. S1 . High variability is observed in most of the studied cases, attributed to the measurement technique and multiple sample handling, from the manufacturing, boxing, shipping and unboxing 13 . The reference masks ( Supplementary Fig. S1a ) themselves also display a strong standard deviation. The mean value varies between − 484 and − 375 V with a global standard deviation of 127 V. These values were used as the point of comparison for the rest of the measurements, represented by a dashed blue line for the mean and a light orange shaded area for the standard deviation in the figures. The results of the different folding treatments indicate a slight shift in the level of the electrostatic potential (Fig. 4a) , which does not increase with the number of folding cycles. The saliva and sweat treatments had a greater impact on the electrostatic charge of the masks; the effect increases with the duration of immersion, as shown in Fig. 4b , c. The combination of two treatments (i.e., saliva or sweat plus folding) has a similar impact as sweat and saliva treatments only. On the other hand, in-situ treatments have a considerable effect on the electrostatic potential of the masks considering that they were not directly immersed Figure S2a shows the FE of the reference masks together with the deviation within the same batch. Mask FE follows a similar trend as the surface potential modification after the various treatments. The folding cycles have a small effect on the FE (Fig. 5a ) whereas sweat and saliva ageing induce a decrease of FE for sub-micron particle sizes (Fig. 5b, c) . The same effect is noticed on the FE analyses performed after in-situ treatments, with a decrease from 97 to 92% and 93% at 1 µm for sweat and saliva, respectively. The results of the washing cycles on Fig. 5d show a strong effect on the global FE of the mask: a large drop comparable to that induced by the discharge storage in liquid isopropanol is noticed with a global loss of 35% in filtration efficiency. Based on the breathability tests, microstructural observation, and mechanical test results ( Fig. 2 ), we show that folding and body fluid treatments do not significantly impact the microstructure nor the mechanical integrity of the mask. All results on the treated masks, however, show an increased variability as compared to reference data; this is attributed to handling and manipulation of the masks. The micrographs confirm these results, showing a similar fiber distribution and pore size. The mechanical integrity of the whole mask is provided by the outer and inner layers as the middle layer can sustain significantly lower levels of stress. While most treatments do not yield any statistically significant change, the 100 folding cycles led to a degradation of Young's modulus by 25% with respect to the reference values, indicating a loss in connectivity of the fiber network. The washed masks exhibit a slight increase in air permeability (8.5% at 200 Pa), implying a change in their integrity. The micrographs of the middle layer of the washed mask in Fig. 3 , show a non-uniform fiber distribution and a larger pore size compared to the reference; likely this is the cause for the observed increase in air permeability and lower strain at break of the layer. As the electrostatic potential is one of the main filtering mechanisms for particles of diameter below 300 nm 14 , the filtration performance of the masks and the measured electrostatic potential are strongly correlated. The folding treatment has no effect on the mask's mechanical integrity and no charge exchange could have occurred, so the FE is not impacted. Adversely, sweat and saliva impacted both the electrostatic potential and the FE after 8 h of ageing. Neither of the two solutions is pH neutral; the saliva is acidic with a pH of 6 while the sweat is more basic within the range of 8, implying that respectively excess H + and OHions are present in these solutions. Biermann et al. 15 suggested that charge exchange between the charges of the mask and the ions of the solutions could take place; this could also be due to an increase in the charge mobility caused by a higher electrical conductivity of the solution 16 . The in-situ treatment has a considerable effect on the electrostatic potential and the FE (Figs. 4, 5) . This could be induced by the spraying while exposing the pores during folding, and by the liquid being pushed into the mask during the compression phase, inducing a full coating of the mask fibers by the solution/body fluid. The washed masks and the isopropanol treated ones are comparable in their strong loss in FE. Isopropanol is known to suppress all the electrostatic charges; by comparison with the FE of the washed masks, it is confirmed that the charges are also annihilated in this scenario 10 . Washed mask fabrics become inefficient for the filtration of fine aerosols. Washing is thus not recommended, due to the loss in fine particles filtration, even if the filtration Table 2 . For each type of treatment, list of the coefficient of determination R 2 of the permeability fitted curves in Fig. 2a along with the derived Pressure drop for an airflow of 8 L min −1 . In the last three columns, details of the derived mechanical properties for each treatment and layer: Young's modulus, ultimate tensile strength and strain at break (also presented in Fig. 2c -e). www.nature.com/scientificreports/ of larger particles, above 3 µm as recommended in the BFE measurement is not affected 11 , whereas prolonged wear and handling can be accepted if the risk of viral contamination is not high. This study provides an evaluation of the durability of Type IIR medical masks under prolonged public use, above the usual recommendation of 4-h wear time and without an immediate discarding the mask. The methods developed for this study are based on our interpretation of potential mask usage adapted to reproducible laboratory conditions. To ensure comparison of the masks' filtration efficiency results for each treatment, all masks were taken from the same batch and same provider as this measurement is highly dependent on the mask source and lot number. Therefore, this study only highlights the relative effects of handling and wear on the efficiency of the mask, for a commercial mask of good initial intrinsic quality. To keep the limit of one batch testing, only one mask was tested per scenario for the filtration efficiency and three samples were cut from each mask, restricting the statistical value of the study, which was meant to provide first quantifications and evaluation of risks. Finally, this study only assessed the role of the polypropylene (PP) non-woven material and did not consider mask fitting related issues, or any viral contamination effects. As a conclusion, we assessed the durability of Type IIR surgical masks after simulated use, probing the possibility of prolonged use in case of mask shortage, as well as limiting the environmental burden of this single-use www.nature.com/scientificreports/ plastic by increasing its use time 17, 18 . The main outcomes of the current study are thus that ageing in saliva or sweat and folding only exert a minor impact on the efficiency of the mask. As a result, masks can be worn for more than the usually recommended 4 h. Also, provided that any potential viral or bacterial contaminations of the mask can be strongly reduced after a given storage time, and that the masks are not visibly soiled or damaged, the strategy to store and reuse surgical masks as proposed by several research groups seems feasible, even if the masks have been handled without particular care, in contact with sweat and saliva, and folded several times. In parallel, we evaluated the washability of these masks through the same protocol. The effect of washing the mask can be interpreted in light of the European standard EN 14683 and BFE, as reported recently, with the conclusion that the mask still comply after 10 cycles of washing 11 .On the other hand, our study confirmed the risk of reduced efficiency for particle sizes below a few µm 19 . Considering the extended use of the surgical masks and the potential role of fine aerosols in the COVID-19 spread, we suggest that washing the masks should be avoided, even though their mechanical integrity is rather well preserved, as their filtration of fine aerosols is greatly reduced due to disappearance of all electrostatic interactions. Materials. Type IIR, non-woven polypropylene (PP) premium face masks with elastic earloops from Care & Serve ® (WIROS Gmbh, Germany) 20 were used. They are composed of three layers made of synthetic PP fibres: outer, filter/middle and inner (face contact) layer. The outer layer is made of hydrophobic non-woven spun-bonded PP which prevents the penetration of droplets through the mask, avoids the inhalation of droplets by the user, and provides splash resistance. The filter, inner, layer is made of a densely packed fine fiber layer of meltblown non-woven PP providing the main filtration and barrier functions, resulting in a bacterial filtration efficiency of 98% for a mean particle size of 3 µm 21 . Furthermore, this layer is electrostatically charged to block very fine particles and viruses. Finally, the inner layer is made of hydrophilic non-woven spun-bonded PP to block the droplets ejected by the user during exhaling, speaking, etc.…, and avoid their penetration through the mask. The spun-bond inner and outer layers also provide the mechanical integrity of the mask. The microstructure of the different layers is presented in Fig. 1a highlighting their difference in pore size and structure. Ageing. During use, the mask is in contact with body fluids, such as saliva and sweat. To simulate fluid interaction induced mask ageing, a protocol was conceived with artificial sweat or saliva. Artificial sweat was prepared according to the formulation by Ferell et al. 22 , and artificial saliva following Klimek et al. 23 . The masks were aged by immersing in one of the solutions for a pre-determined amount of time that ranged from 1 h to 1 week. Subsequently, the masks were dried at ambient conditions for one day. A set of masks was also subjected to a folding treatment after ageing and drying. The compounds and their providers are listed in Supplementary Tables S1 and S2. In order to remove all the electrostatic charges and to obtain only a mechanical filtration, some masks were discharged according to EN 779 24 by isopropanol (IPA) liquid discharging. Combined in-situ treatment. Folding and ageing treatments were performed on separate sets of masks. However, in real life the folding and fluid interaction take place successively. To simulate this, we combined the fold- www.nature.com/scientificreports/ ing and ageing treatments as follows: the mask underwent several compression/decompression cycles; before each compression step, both sides of the mask were sprayed with either the artificial saliva or the artificial sweat. Once the process was performed the masks were dried under a fume hood. Washing. The washing treatment was performed with detergent powder (10 g/L ECE non-phosphate reference detergent) according to the ISO 6330:2012 but with a household washing machine (Adora L from V-Zug AG) instead of a standardized testing machine. It consisted of 10 washing cycles at 60 °C, for 45 min. In total, 6 masks were washed: three of them were used for the mechanical and air permeability tests; the rest was tested for their filtration efficiency. Table 1 gives an overview of performed treatments and the number of masks treated. Characterization methods. Microscopy. A Keyence VHX-7000 microscope equipped with a 100 × lens was used to observe the microstructure of mask layers. Treated and untreated masks were analyzed to assess the effects of the different treatments on the microstructure of the spunbond and meltblown PP layers. A scanning electron microscope GeminiSEM from Zeiss was used in High Vacuum mode to observe the structure of a reference and a washed mask. Prior to observation, the samples of individual layers were coated with gold to a thickness of 50 nm. Permeability test/breathability test. Breathability was assessed by air permeability measurement using a Textest FX 3320 MOBILAIR test bench. Each specimen was tested at 5 different locations with an area of 5 cm 2 as shown in Fig. 1b . Measurement sites were chosen at an adequate distance from the edges of the mask avoiding overlap between the test locations; measurements took place after unfolding the plies. Each site had its air permeability measured at three different pressure levels: 50, 98 and 200 Pa, to fit the permeability as a linear function of the input pressure. From the obtained fit, it was possible to derive the breathability, characterized by the pressure drop, ΔP, according to EN14683, i.e., at a flow rate of 8 L min −1 on a surface of 4.9 cm 2 which is equivalent to an air permeability of the material of 272 L m −2 s −1 . According to this standard, the pressure drop should be below 60 Pa cm −2 for Type IIR masks 25 . Three masks for extreme cases of folding (100 cycles) and ageing (1 week in saliva or sweat) were tested as compared to reference masks. Mechanical characterization. Each layer of reference and treated masks were characterized in uniaxial tensile mode with a Zwick uniaxial testing machine equipped with a 100 N load cell. The samples were extracted by direct die-cutting of the layers in the horizontal direction from individual layers of the studied masks in a dog bone shape with a length of 75 mm and a width of approximately 4 mm as shown in Fig. 1c . Prior to the tests, the exact width and the thickness of the samples were measured using respectively an optical microscope Keyence VHX-7000 and a micrometer screw gauge. The specimens were tested in tensile mode at a displacement speed of 50 mm min −1 until specimen failure during which both the displacement and the force were recorded. From the data acquired, the engineering stress-strain curves of the different specimens were derived. The acquired data were then used to extract the mechanical properties of the three layers of the different masks, such as the tangent Young's modulus taken at 0.2% of strain, the ultimate tensile strength as well as the deformation that the layers undergo before failure. As for the permeability tests, the three extreme configurations were tested to assess their impact on the mechanical properties of the mask layers. For each configuration and the reference mask, 9 samples from 3 different masks (3 per mask) were cut out. Surface potential measurement. The surface potential of the masks was characterized using an electrostatic voltmeter (Monroe 244A) connected to a computer, as described by He et al. 26 . To perform the measurements, the masks were placed on a grounded metal platform and the probe of the electrostatic voltmeter was set at 5 mm above the specimen. Once all was set, the electrostatic potential of the mask was measured at 5 different positions on the mask to evaluate the mean electrostatic potential of the entire surface of the mask. Each measurement was performed on a circular area with a diameter of 10.5 mm. The side on which the measurement was conducted was shown to affect the results. This characteristic is attributed to the different structure and treatments of the inner and outer layers (hydrophilic vs. hydrophobic). The electrostatic charge of the mask was thus systematically measured from the outer layer, for comparison purposes. The five measurements were performed approximately at the same position as the permeability tests (Fig. 1d) . Filtration efficiency (FE). Circular specimens with a diameter of 60 mm were sampled from masks and hermetically sealed in a sample holder to obtain an effective test surface of 1.66 × 10 3 mm 2 . An aerosol was drawn with an aerosols generator (AGK2000) from a solution at 0.02 g/mL of fructose in demineralized water. Fructose particles at a concentration of 35 mg m −3 in dried air were neutralized in a corona discharging unit (CD2000) and driven to the sample. A constant airflow of 8 L min −1 was exerted through the mask specimen (from outside to inside); particle penetration was quantified using the particle analyzer Cambusion DMS500 (see Fig. 1e ). This "Fast Particulate Spectrometer" uses unipolar corona charging and parallel detection of particles of varying electrical mobility to offer real-time measurement of the particle size spectrum between 5 and 2500 nm. The FE is determined by comparing a steady flow of particles after a constant concentration is reached during 2 min with the mask sample, then measuring the raw gas concentration during another minute without the mask sample. FE is expressed as a percentage and is reported in the particle range from 100 to 1200 nm, averaged COVID-19 infection: Emergence, transmission, and characteristics of human coronaviruses Clinical features of patients infected with 2019 novel coronavirus in Wuhan COVID-19: Transmission, prevention, and potential therapeutic opportunities Facemask shortage and the novel coronavirus disease (COVID-19) outbreak: Reflections on public health measures Testing the efficacy of homemade masks: Would they protect in an influenza pandemic? Evaluation of cloth masks and modified procedure masks as personal protective equipment for the public during the COVID-19 pandemic Contamination by respiratory viruses on outer surface of medical masks used by hospital healthcare workers An overview of filtration efficiency through the masks: Mechanisms of the aerosols penetration Aerosol filtration efficiency of common fabrics used in respiratory cloth masks Recharging and rejuvenation of decontaminated N95 masks Reuse of medical face masks in domestic and community settings without sacrificing safety: Ecological and economical lessons from the Covid-19 pandemic Development of antituberculosis melt-blown polypropylene filters coated with mangosteen extracts for medical face mask applications Aerosol filtration efficiency of household materials for homemade face masks: Influence of material properties, particle size, particle electrical charge, face velocity, and leaks Progress in electrospun nanofibres for air filtration Evaluation of permanently charged electrofibrous filters Effect of solvent exposure on the filtration performance of electrostatically charged polypropylene filter media What is the environmental impact of different strategies for the use of medical and community masks? Cotton and surgical masks-What ecological factors are relevant for their sustainability Face masks in the new COVID-19 normal: Materials, testing, and perspectives Medical face masks-Requirements Specification for performance of materials used in medical face masks Perspiration poisoning of protective clothing materials. Part I. Mathematical model for a complex adsorption bed Fluoride taken up by plaque, by the underlying enamel and by clean enamel from three fluoride compounds in vitro Particulate air filters for general ventilation-Determination of the filtration performance Community face coverings-Guide to minimum requirements, methods of testing and use Filtration performance and charge degradation during particle loading and reusability of charged PTFE needle felt filters This work was funded by the Swiss Innovation Agency Innosuisse, as part of the project "Remask: strategies for innovations for Swiss masks in pandemic situations", number: 46668.1 IP-ENG. LPAC would like to thank AISTS for lending their air permeability testing apparatus, and the Interdisciplinary Centre of Electron Microscopy (CIME) for SEM imaging. The authors would like to acknowledge many online fruitful discussions and advice from members of the reMask group of the Swiss National COVID-19 Science Task Force. The authors declare no competing interests. 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