key: cord-0908570-kkkrkgg1
authors: Belsy, Acosta; Odalys, Valdés; Alexander, Piñón; Clara, Savón; Angel, Goyenechea; Grehete, Gonzalez; Guelsys, Gonzalez; Luis, Sarmiento; Pedro, Más; Guadalupe, Guzmán María; Alina, Llop; Pilar, Perez Breña Ma; Inmaculada, Casas
title: Molecular characterization of adenoviral infections in Cuba: report of an unusual association of species D adenoviruses with different clinical syndromes
date: 2009-03-12
journal: Arch Virol
DOI: 10.1007/s00705-009-0338-4
sha: 465dbf79b3406de602dd16228f8c071abd53d9e5
doc_id: 908570
cord_uid: kkkrkgg1

Adenoviruses are common pathogens that are responsible for a wide variety of infectious syndromes. The objectives of this study were to identify and characterize members of different adenovirus species at the molecular level and to describe the correlation between viruses and clinical syndromes during a period of 4 years. Between 2002 and 2006, 45 of 512 respiratory specimens (8%) from patients with acute respiratory tract infection tested positive for adenovirus. Four adenovirus isolates from samples sent for enterovirus isolation were also analyzed. This research identified 49 confirmed cases of human adenovirus infection by PCR and/or viral culture. The most common diagnosis was upper respiratory infection (44%). Human adenovirus D was the major species found (59%), followed by Human adenovirus C (36%) and Human adenovirus B (4%). Human adenovirus 5 was the major serotype found producing bronchiolitis, followed by human adenovirus 6. In patients with upper respiratory infection, the major serotype found was human adenovirus 17. Viruses of the species Human adenovirus D were identified in seven (77%) cases of acute febrile syndrome. Four isolates from clinical materials obtained from patients with encephalitis, acute flaccid paralysis and meningoencephalitis were identified as belonging to the species Human adenovirus D. Our data demonstrate a surprising result about the identification of an unusual association of viruses of the species Human adenovirus D with different clinical syndromes. This observation could be evaluated as a possible indicator of the emergence of a novel strain but further studies are required.

Human adenoviruses (HAdVs) are members of the family Adenoviridae, whose members infect hosts across a broad spectrum of vertebrates. There are 51 serotypes of HAdV in the genus Mastadenovirus, distributed in six species, A-F (formerly subgroups or subgenera) on the basis of their physicochemical, biological and genetic properties. A new serotype isolated recently (52) has been proposed to represent a new species G [21] .

Human adenoviruses are associated with sporadic infection, and community and institutional outbreaks. These viruses cause a variety of clinical manifestations, such as conjunctivitis, pneumonia, gastroenteritis, and hemorrhagic cystitis. Some serotypes can occasionally infect tissues of the central nervous system (CNS) and cause aseptic meningitis, meningoencephalitis, and encephalitis. They can cause especially severe disease in infants, young children, immunocompromised persons, and transplant recipients [2, 5, 14, 15, 25, 30, 31] .

Serosurveys suggest that virtually all people are exposed to HAdV during childhood [22, 35] . They can remain in an asymptomatic carrier state until at least young adulthood [18] , and virus may be actively shed long after symptomatic infection [16] .

Over the past decades, neutralization tests, ELISA, and virus isolation had been used for the detection and identification of adenovirus serotypes. However, these methods are relatively complicated, labour-intensive, and timeconsuming, and they have low sensitivity. [26, 39, 42] . These disadvantages have limited their use. Amplification of the viral genome by PCR has been introduced as a convenient and powerful alternative for molecular diagnosis. Additionally, genome amplification allows further characterization of the adenovirus serotype by sequence analysis [1, 7, 34] .

The objectives of this study were the identification and molecular characterization of different HAdV isolates and to describe the correlation between viruses and clinical syndromes during a period of 4 years.

Between October 2002 and September 2006, 512 respiratory specimens (nasopharyngeal swabs and pharyngeal washes) from patients with acute respiratory tract infection (ARTI) were sent specifically to the National Reference Laboratory of Respiratory Viruses for testing respiratory viruses, including influenza virus A, B and C, human respiratory syncitial virus (HRSV), human parainfluenza virus 1-4, HAdV, human coronavirus, human rhinovirus and human metapneumovirus (HMPV). Routine virological testing for respiratory pathogens was performed using a combination of direct immunofluorescence, isolation in cell culture and PCR assays [9, 10, 27] . In addition, four samples (three stools and one cerebrospinal fluid) from the enterovirus (EV) laboratory with previous viral isolation without identification were analyzed. Nasopharyngeal swabs and pharyngeal washes were collected in 3 ml of virus transport medium (MEM, Gibco-BRL, Life Technologies, Paisley, Scotland; penicillin 200 U/ml, and streptomycin 200 lg/ml, BioWhittaker, MA; mycostatin 200 U/ml, Sigma; bovine serum albumin 0.25%, Merck, Darmstadt, Germany) The specimens were frozen and stored at -70°C until the analysis was carried out.

A human embryonic fibroblast cell line was used for primary isolation of EV. Tubes with 80% confluent monolayers were inoculated with 0.2 ml of homogenized samples. Cells were fed with 2 ml of 2% fetal calf serum in Basal Medium Eagle and visualized for cytopathic effect (CPE) twice a week. When a CPE that was not typical of EV was observed, the monolayer was scraped and tested for adenovirus antigen by immunofluorescence with a specific monoclonal antibody (Chemicon, Temecula, CA).

Total viral RNA/DNA from 200-ll aliquots from clinical samples or supernatant of infected cell culture was extracted using the guanidinium thiocyanate method as described previously by Casas et al. [6] . The lysis buffer included 100 copies of the cloned, amplified product of the internal control described by Coiras et al. [9] . It was used for checking the extraction process, the amplification efficiency, and the presence of inhibitors in the clinical specimens. After processing, the dried pellet was resuspended in 15 ll of RNAse-free sterile water. Negative controls consisting of RNase-free sterile water (Sigma) were treated following the same procedure. For each assay, known positive controls, derived from infected viral cells, were added.

In all cases, nucleic acid extracts (5 ll) were examined for influenza virus A, B and C, HAdV, HRSV A and B [9] , human parainfluenza virus 1-4, coronaviruses, rhinoviruses, and EV [10] , using nRT-PCR. In addition, a multiplex nPCR assay for human herpesvirus was used in the case of a patient with encephalitis [40] . The protocols employed have been published previously and used for clinical diagnosis. Three different biosafety cabinets were used for master mix preparation, sample handling and primary reaction product handling. Different laboratory wear and coats were used for each cabinet. Amplicon detection was done in a different room.

Specimens that were positive for HAdV were processed by two independent nested reactions with 20 pmol of ADHEX2F, nt 20485 to 20503; (5 0 CCCITTYAACCACC ACCG3 0 ) and ADHEX1R, nt 20836 to 20854; (5 0 KATG GGGTARAGCATGTT3 0 ) or 20 pmol of ADHEX1F, nt 20380 to 20400; (5 0 CAACACCTAYGASTACATGAA3 0 ) and ADHEX2R, nt 20632 to 20652; (5 0 ACATCCTTBC KGAAGTTCCA3 0 ) degenerate primers as described previously [7] . These nested primer pairs were designed to bind inside the hexon protein coding region of the adenovirus genome [1] . The PCR products were purified with QIAquick PCR purification kit (Qiagen) according to the manufacturers's protocol and were sequenced in both directions using the primer pairs described above.

Sequences were obtained using an automatic DNA sequencer (ABI Prism 3700; Applied Biosystems) and a Big Dye Terminator Cycle Sequencing kit version 3.1 (Applied Biosystems). The fragment size sequenced corresponded to 122 amino acids, from amino acid position 540-662 of the hexon protein. This fragment is located at the external surface L2 of the hexon protein monomer but does not overlap with the HRV7 domain [11] .

The nucleotide sequences obtained were first analysed using the CHROMAS software (version 1.3). The forward and reverse sequences were combined using BioEdit and were compared and aligned with the corresponding previously published sequences of prototype viruses available from GenBank, using the CLUSTAL X (version 1.83) program. Specimens that yielded identity scores C90%, were considered to be good genotype matches. Phylogenetic trees were inferred using programs from the MEGA package (version 4) and reconstructed using the neighbourjoining method. The evolutionary distances were estimated using Kimura's two-parameter method [24] . The statistical significance of a particular tree topology was evaluated by 1,000 replicates of boostrap re-sampling. The criteria for serotype assignation were the shortest distance from a prototype strain, as deduced from the phylogenetic tree ( Fig. 1) , and the highest similarity value obtained after sequence comparisons.

The GenBank accession numbers of the nucleotide sequences presented in this study are: EU179755-EU179763, EU179765, EU179768-EU179785 and EU439569-EU439589.

In the present report, the nested PCR method used was able to detect different HAdVs in clinical samples and supernatant culture with a sensitive internal control system to assure the quality of reaction conditions in each individual tube.

In this investigation, 49 confirmed cases of HAdV infection were identified by PCR and/or viral culture ( Table 1) .

The monthly collection of clinical samples is shown in Table 1 . Human adenovirus was detected throughout the year; however, 38 samples (77%) were collected between June and September. Two significant outbreaks of HAdV infection were identified, one in July of 2005 and the other in September of 2006.

More than half of the positive samples (53%) were from children younger than 5 years of age, and 50% of them were under 6 months of age. Thirty-two percent were from patients between 5 and 20 years, and 14% were from adults between 21 and 64 years of age. No difference in gender distribution was observed.

The most common diagnosis was upper respiratory infection (44%). Medical records documented the following symptoms at initial presentation: nasal congestion (98%), sore throat (83%) and cough (78%). All patients were febrile. Fourteen (28%) patients had bronchiolitis. Acute febrile syndrome (18%) was defined as the concurrence of fever, cold symptoms, headache, weakness, vomiting and diarrhoea, a common but non-specific symptom. In these cases, the results of diagnostic assays for the detection of other potentially pathogenic enteric microorganisms were negative. An interesting finding was the detection of HAdV in a patient 64 years of age who was admitted with a diagnosis of encephalitis. No human herpesvirus, influenza virus, EV or flavivirus genomes were found in the cerebrospinal fluid of this patient. Human adenovirus DNA was detected in the supernatant of a cell culture infected with viruses obtained from fecal specimens taken from a patient with acute flaccid paralysis (AFP), as well as in two cases of meningoencephalitis. Overall, 73% of HAdV positive patients were hospitalized.

Partial hexon nucleotide sequences for 49 different HAdV types were obtained. A BLAST search in the GenBank database for all of the amplicon sequences determined in the present study was done. A 100% agreement with existing GenBank sequences for serotypes 1, 3, 5, and 6 was obtained. An agreement ranging from 99 to 100% was obtained for prototype strains of Human adenovirus D. An alignment of the hexon gene sequences was computed by using sequences from representative serotypes of species B (HAdV 3), C (HAdVs 1, 5, and 6), and D (HAdVs 9, 10, 13,15,17, 19, 23, 25, 28, 30, 32, 33, 37-39, 43, 44, and 47-49) . In order to evaluate the phylogenetic relationships between individual prototype viruses and positive cases of HAdV infection by PCR and/or viral culture, phylogenetic trees were constructed using a phylogeny reconstruction algorithm (the neighbour-joining method) and a nucleotide substitution method . Phylogenetic and sequence similarity analysis permitted accurate species classification and serotype identification except for species D HAdV, for which serotypes could not be clearly defined.

The resulting phylogenetic tree showed three different clusters, represented by the species B, C and D. The bootstrap values ( Fig. 1) were, in general, quite high, and especially at nodes between species. However, these values were less good for some nodes within species D. The assignment of a serotype to a clinical isolate was done by calculating the shortest distance between the isolate and a prototype strain. A total of 31 (63%) HAdVs were serotyped, and typing results yielded some interesting observations.

The major species found was HAdV-D, with 29 clinical cases (59%), followed by HAdV-C, with 18 cases (36%), and HAdV-B, with two cases (4%).

In bronchiolitis cases, HAdV-5 (71%) was the major serotype, followed by HAdV-6 (14%) and single cases of HAdV-1 and HAdV-D. In the group of patients with upper respiratory infection, the major serotype found was HAdV-17 (77%). HAdV-5 (22%) was the second-most common serotype, and five specimens were typed as HAdV-D; one of them recovered in 2003 and four in 2005. Lastly, two specimens recovered in 2003 were HAdV-3. HAdV-D was the species identified in nine (100%) cases detected during an atypical outbreak of acute febrile syndrome in July of 2005. Four isolates from clinical materials obtained from patients with severe disease such as encephalitis, AFP and meningoencephalitis were identified as HAdV-D.

HAdVs are categorized by species (HAdV-A, HAdV-B , HAdV-C, HAdV-D, HAdV-E and HAdV-F), and further by serotype (Ad1-Ad51), and cause a wide spectrum of illnesses in both children and adults, including those involving the CNS. A new recently isolated serotype (52) has been proposed to belong to a new species G [3, 13, 21] .

More than half of the positive samples (53%) were from children younger than 5 years of age, and 50 % of them were under 6 months of age. The highest incidence of adenoviral infection occurs in children from 6 months to 5 years of age, although outbreaks have been noted when susceptible hosts, such as military recruits, adolescents, visitors to summer camps, and sometimes nursing home residents, congregate together. Explanations for the relative lack of illness in the youngest or in older individuals include the presence of transplacentally acquired maternal antibody in young infants and the development of neutralizing antibody to the most common adenoviral strains in the majority of children older than 5 years old.

The age distribution of HAdV in developing countries appears to be similar to that in developed countries [8] . The results in this study showed that adenovirus infections were more common in children younger than 5 years of age, and 50% of them were under 6 months of age. Most patients were hospitalized (73%); nevertheless, none of these had severe respiratory infections. In Cuba, infants are considered special patients by pediatricians.

This report also describes the seasonal variation of adenovirus infections in Cuba. Respiratory viral agents usually have characteristic seasonal patterns in temperate and tropical climates. In temperate climates, the majority of isolations of respiratory viruses are in the winter. In Central America, there are few studies published about the epidemiological characteristics of adenoviruses. The Cuban island is located in the Caribbean Sea at the entrance of the Gulf of Mexico. The climate of Cuba is semitropical, and two seasons are generally recognized: a rainy season from May to October and dry season from November to April. Cuba is often hit by hurricanes from June to November, resulting in great economic loss and temporarily interrupted sanitary conditions. The average minimum temperature is 21°C (70°F), and the average maximum is 27°C (81°F). The warmest month is July with 30°C (86°F). In this study, adenovirus was detected throughout the year; however, two significant outbreaks of HAdV infection were identified, the first in July of 2005, when Hurricane Dennis hit severely the western part of Cuba, and the second in September of 2006. We believe that the effect of temperature and rainfall appears to be a determinant of the timing of those outbreaks. Figure 1 displays a phylogenetic tree built from an alignment of the nucleotide sequences of the amplicons obtained from clinical samples and each serotype prototype. As shown in Fig. 1 , each serotype is clearly distinct. Serotypes belonging to the same species cluster together.

In Cuba, human adenovirus circulates in the pediatric population throughout the year. Furthermore, members of adenovirus species C of are commonly involved in respiratory diseases in the pediatric population. Two previous studies have been reported on the associations between specific clinical syndromes and HAdV serotypes. The analysis of the occurrence of HAdV-C revealed that HAdV-1 and HAdV-6 were the predominant serotypes in children with acute respiratory diseases, and HAdV-37 was the most prevalent one associated with conjunctivitis [36, 41] .

Human adenovirus respiratory infections have usually been associated with species B, C, and E [8] . HAdV-C includes HAdV-1, HAdV-2, HAdV-5, and HAdV-6. These serotypes are commonly associated with febrile respiratory illness in children and are noted to be endemic in certain regions or in epidemics [32] . The significant proportion of infection with HAdV-1, HAdV-3, HAdV-5 and HAdV-6 among patients with respiratory infection is consistent with previous reports [7, 20, 23, 32] . However, we detected HAdV-D in a child with bronchiolitis, and in 15 cases of upper respiratory infection. HAdV-D are rarely isolated in respiratory illness surveys, and strong causal correlations are generally lacking. Nevertheless, this association was previously described by Casas et al. [7] . On the other hand, HAdV-D was identified in a group of patients with acute febrile syndrome and in four isolates from clinical materials obtained from patients with encephalitis, AFP and meningoencephalitis. Sporadic cases or small outbreaks of neurological disease following adenovirus infection are well documented [12, 17, 25, 38] . The high prevalence of infection with HAdV-D in previously healthy patients is not consistent with previous reports. This association was unexpected, and a detailed analysis of this sample set will be published in a separate report.

Human adenovirus is unique among the common respiratory viruses in that it can spread to organs other than the respiratory system, resulting in conjunctivitis, gastroenteritis, acute hemorrhagic cystitis, and meningoencephalitis. The liver, spleen, pancreas, kidney, or heart may also be involved in disseminated infections, both in previously healthy people and in immunocompromised patients (e.g., infants, patients with AIDS, transplant recipients) [33] . HAdV-D has been frequently associated with severe adenovirus infection in these patients. In our study, we reviewed the medical records and did not find that the patients suffered from AIDS or another type of immunodeficiency. However, we thought that is important to take into account that the highest percentage of HAdV-D was identified in children.

The identification of such a large number of HAdV-D infections is extremely interesting and a major and unique finding. We recognize that the limited sequence data analyzed (370 nt in a relatively conserved region of the hexon) for typing did not generate enough results. The sequencing of the hypervariable regions of the hexon gene (HVR7 alone or HVR1-6) and fiber gene of select strains will certainly provide a solid basis for confirming the species/serotype identification [29, 37, 43] . We used the method previously published by Casas et al. [7] , to facilitate sequencing and typing of HAdVs using the hexon protein coding region of the HAdV genome. This method was extensively validated using clinical samples and prototype strains, and we also noted unusual associations between specific serotypes and clinical presentation. The primers and PCR conditions have also been extensively validated. Some years ago, adenovirus infection was considered to have little consequence. However, much has changed since these early epidemiological studies were conducted. In contrast to the modest number of HAdVs recognized 35 years ago, 51 unique serotypes are now recognized, and a new serotype isolated recently (52) has been proposed to belong to a new species G. Different serotypes have been found to have different tissue tropisms that correlate with different clinical manifestations of infection. Partial epidemiological investigations have revealed that, among some specific serotypes, multiple genetic variants exist that often have quite different geographical distributions and associated virulence [19, 21, 28, 30] .

Our study expands the range of HAdV serotypes that have been reported elsewhere in association with major clinical syndromes. Further independent testing is needed to verify these associations. However, future studies of HAdV should not exclude these rare serotypes, and they should be kept in mind.

Unfortunately, because adenoviruses can be asymptomatically shed for prolonged periods of time, recovery of HAdV from the upper respiratory tract or stool samples by culture does not confirm it as the cause of a specific disease. Accordingly, recovery of HAdV should always prompt an effort to identify any additional or alternate potential explanations for symptoms present at the time of a positive viral culture [4, 16] . Additional research is needed for the development of better rapid methods to detect and to quantify HAdV in various body fluids (blood, stool, and throat).

Finally, our findings, combined with the existence of several reports concerning the association of HAdV with different syndromes confirm that members of the family Adenoviridae, mainly the serotypes belonging to HAdV-C are common causal agents of respiratory infection. In contrast, serotypes of HAdV-D could be involved in the aetiology of acute respiratory infection and neurological disorders in previously healthy people. However, this observation needs to be confirmed in a larger study.

Our identification of HAdV-D associated with different syndromes may signify the emergence of new genomic variants that have the potential to spread globally. Further studies of such typing with other molecular typing methods are necessary. These reports show that surveillance, serotyping and molecular characterization methods need to be improved in order to identify emerging adenovirus variants.

authors wish to thank all of the physicians who provided samples and clinical data. We also acknowledge all technicians and researchers of the Virology Deparment of the Instituto de Medicina Tropical Pedro Kourí, la Habana, Cuba, who collaborated with this investigation. Thanks to Dr Enrique Tabarés, from Universidad Autónoma de Madrid, Spain, for his help.

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Acknowledgment This work was supported in part by the Cuban National Program of Surveillance and Control of Acute Respiratory Infection. The nucleotide sequence was performed in the Instituto de Salud Carlos III, Madrid, Spain, and was funded by the proposal MPY-1177/06 entitled Caracterización molecular de HAdV detectados en muestras clínicas de pacientes con infección respiratoria. The