key: cord-0898588-8dyaeu2v
authors: Piralla, Antonio; Zecca, Marco; Comoli, Patrizia; Girello, Alessia; Maccario, Rita; Baldanti, Fausto
title: Persistent rhinovirus infection in pediatric hematopoietic stem cell transplant recipients with impaired cellular immunity
date: 2015-06-30
journal: Journal of Clinical Virology
DOI: 10.1016/j.jcv.2015.03.022
sha: 40276a234b15f83ca6f70b4956659025fd7b2ae5
doc_id: 898588
cord_uid: 8dyaeu2v

Abstract Background HRV infections are generally self-limiting in healthy subjects, whereas in immunocompromised hosts HRV infections can lead to severe complications and persistent infections. The persistence of HRV shedding could be due to the inefficient immunological control of a single infectious episode. Objectives To investigate the clinical, virologic and immunologic characteristics of pediatric HSCT recipients with HRV-PI infection. Study design During the period 2006–2012, eight hematopoietic stem cell transplant (HSCT) recipients presented with persistent rhinovirus infection (HRV-PI, ≥30 days). Viral load and T-CD4+, T-CD8+, B and NK lymphocyte counts at the onset of infection were compared with those of fourteen HSCT recipients with acute HRV infection (HRV-AI, ≤15 days). Results The median duration of HRV positivity in patients with HRV-PI was 61 days (range 30–174 days) and phylogenetic analysis showed the persistence of a single HRV type in all patients (100%). In HSCT recipients with HRV-PI, T-CD4+, T-CD8+ and NK cell counts at the onset of infection were significantly lower than those observed in recipients with HRV-AI (p <0.01), while B cell counts were similar in the two groups (p = 0.25). A decrease in HRV load was associated with a significant increase in T-CD4+, T-CD8+and NK lymphocyte counts in HRV-PI patients (p <0.01). Conclusions This study suggests a role for cellular immunity in HRV clearance and highlights the importance of its recovery for the control of HRV infection in HSCT recipients.

Human rhinoviruses (HRVs) have been previously classified into two species, HRV-A and HRV-B, while a distinct rhinovirus group (HRV-C) was recently identified in patients with acute respiratory infections (ARIs) [1] [2] [3] [4] [5] [6] . HRV-C and HRV-A are more frequently associated with ARIs in hospitalized patients and often co-circulate in near-equivalent proportions, while HRV-Bs are often underrepresented [4, 6, 7] .

Even though ARIs caused by HRVs are generally self-limiting in healthy subjects, in hematopoietic stem cell transplant (HSCT) recipients [8, 9] and patients with hematological diseases [10] HRV infection may lead to severe complications such as pneumonia [9, 11, 12] . In addition, in HSCT [13] [14] [15] and lung transplant recipients, persistent HRV infections have been reported [16] . The persistence of HRV shedding could be due to the inefficient immunological control of a single infectious episode [16, 17] .

Schiffer et al. reported that respiratory virus infections are more frequent and severe in the first 100 days following transplant in patients receiving myeloablative vs non-myeloablative HSCT, despite an overall similar infection rate [18] . These data would suggest an important role for T-cell immunity in the control of HRV infection. However, correlates of severe or persistent HRV infections (HRV-PI) after HSCT are still largely obscure.

This study investigated the clinical, virologic and immunologic characteristics of pediatric HSCT recipients with HRV-PI infection. 

From January 2006 through December 2012, 1687 respiratory samples (936 nasal swabs, 713 nasopharyngeal aspirates, 25 bronchoalveolar lavages and 13 pharyngeal swabs) from 536 pediatric HSCT recipients with acute respiratory syndromes admitted to the Pediatric Hematology-Oncology Unit, Fondazione IRCCS Policlinico San Matteo were collected both at admission and discharge. Follow-up samples were prospectively collected 7-10 days apart from positive patients to monitor viral load until the clearance of HRV or hospital discharge. In the latter case, follow-up samples were collected at subsequent outpatient clinical evaluations. Respiratory samples were routinely tested using a panel of RT-PCR and real-rime RT-PCR assays [13, 19] for human influenza virus types A and B, human metapneumovirus A and B, human respiratory syncytial virus A and B, four human coronaviruses (OC43, 229E, NL63, HKU1), human parainfluenza types 3, human adenovirus, rhinoviruses and enteroviruses.

A further characterization of HRV infection was retrospectively performed on the samples collected prospectively for acute respiratory infection screening. HRV-PI was defined as HRV shedding ≥30 days, and all patients fulfilling the criteria were included in the study. A cohort of pediatric HSCT recipients with HRV-AI (defined as virus shedding ≤15 days), matched for clinical characteristics, was selected as a control group for virologic and immunologic analysis. This retrospective study was performed according to the guidelines of the Institutional Review Board on the use of biological specimens for scientific purposes in keeping with Italian law (art.13 D.Lgs 196/2003).

Patients with rhinitis, pharyngitis, and laryngitis were classified as upper respiratory tract infection (URTI), while patients with bronchitis, bronchiolitis, and pneumonia (characterized by cough, wheezing, and/or dyspnea as well as suggestive X-ray findings) were classified as affected by lower RTI (LRTI).

Following RNA extraction (Nuclisens ® easy MAG TM automatic extractor; BioMérieux, Lyon, France), HRV RNA was quantified by real-time RT-PCR [20] . Results were reported as HRV RNA copies/ml respiratory sample. HRV molecular typing was performed as reported [21] by sequencing a 549 nt fragment spanning part of the 5 NCR and the VP2 gene (nt 534-1083) or using an alternative protocol that amplifies the same genomic region with different primer sets (nt 547-1087) [6] . Sequencing reactions were carried out using primer sets utilized for amplification with the BigDye Terminator Cycle-Sequencing kit (Applied Biosystems, Foster City, USA) and run in an ABI Prism 3100 DNA sequencer (Applied Biosystems, Foster City, USA). Sequences were assembled using the Sequencher software, version 4.6 (Gene Codes Corporation, Ann Arbor, USA).

Nucleotide sequences were aligned using the ClustalW method and a phylogenetic tree was constructed using the neighbor-joining method and the kimura-2-parameter for simultaneously estimating distance among sequences with MEGA software (version 5.05) [22] . Bootstrap values included 1000 replicates. HRV type assignment was defined by the nearest HRV reference strains observed in the phylogenetic tree. The nucleotide sequences used in this study were deposited into GenBank under accession numbers KJ603464-KJ603520.

Serial T-CD4 + , T-CD8 + , B and NK lymphocyte counts were routinely performed in HSCT recipients (both with HRV-PI and HRV-AI), before and after transplant, while patients with hema- tological malignancies (HMs) not undergoing transplant were not routinely tested. T-Lymphocytes were stained with anti-CD45 PerCP-Cy5.5, anti-CD3 APC, anti-CD8 PE, anti-CD4 FITC monoclonal antibodies (mAbs); B lymphocytes were stained with anti-CD19 and anti-CD20 mAbs while NK lymphocytes, defined as CD3-/CD56+ cells, were stained with anti-CD56 and anti-CD3 mAbs (all mAbs were from Becton Dickinson, Mountain View, CA). Appropriate isotype-matched controls were included. Cytofluorimetric analysis was performed by direct immunofluorescence on a FAC-Scalibur flow cytometer (Becton Dickinson, Mountain View, CA).

Statistical analyses were performed using GraphPad Prism version 4.0 (GraphPad Software, La Jolla, CA). The Mann-Whitney U test was used to compare T-CD4 + , T-CD8 + , B and NK cell counts and viral load for different sampling times and patient categories. P values <0.05 were considered statistically significant. 

In all HRV-PI patients (100.0%) a single HRV type was present during the entire shedding period (Fig. 1) 

Among the eight patients with HRV-PI, the median duration of HRV shedding was 61 days (range 30-174 days), while in the fourteen HRV-AI patients, the median duration of HRV shedding was significantly shorter (10 days, range 5-13 days; p = 0.001). All patients with HRV-PI suffered from URTI, while, among patients with HRV-AI, 12 In all HSCT recipients with HRV-PI, infection occurred either during the conditioning regimen (#1, #2, #7, #9, #11) or prior to transplant engraftment (#3, #4, #8). In these patients, followup viral load, T-CD4 + , T-CD8 ± , B, and NK lymphocyte counts were available. All HSCT recipients received intravenous immunoglobulin (Ig) replacement therapy (400 mg/kg) whenever IgG levels were found below 600 mg/dl after the transplant or at the moment of a positive HRV result.

In the eight HSCT recipients with HRV-PI, the onset of HRV infection occurred at a median time of −10.5 days prior to HSCT (range −36 to 65 days); while in the group of patients with HRV-AI, infection occurred at a median of 58 days (range 0-272 days) after HSCT. Viral load as well as T-CD4 + , T-CD8 + , B and NK cell counts of patients with HRV-PI at the onset of infection were compared with those of patients with HRV-AI (Fig. 2 ). Viral load (median 1.5 × 10 6 vs 2.5 × 10 6 RNA copies/ml respiratory sample; p = 0.61) and B cell counts (median 0 vs 40 cells/l; p = 0.25) were not significantly different in HSCT recipients with HRV-PI vs HRV-AI. On the contrary, the number of T-CD4 + lymphocytes (median T-CD4 + 0 vs 95 cells/l; p = 0.02) T-CD8 + lymphocytes (median 0 vs 285 cells/l; p < 0.01) and NK lymphocytes (median 0 vs 301 cells/l; p < 0.01 was significantly higher in HRV-AI patients.

In the eight patients with HRV-PI, the increase in T-CD4 + , T-CD8 + and NK cell counts was paralleled by a decrease in HRV load (Fig. 3) . Of note, in one patient (#7) with persistently low T-CD8 + cell counts (<100 cells/l), HRV load was still high after 135 days of infection. Among patients with HRV-PI, a significant difference in HRV load (median 1.1 × 10 6 vs 0 copies/ml respiratory sample; p = 0.003), T-CD4 + (median 0 vs 115.5 cells/l; p = 0.01) T-CD8 + (median 0 vs 371 cells/l; p = 0.006) and NK (median 0 vs 220 cells/l; p = 0.009) cell counts was observed between the onset of infection and the last positive HRV result or viral clearance (Fig. 3) . In contrast, no difference in B cell counts (median 0 vs 7 cells/l; p = 0.09) was observed between the onset of infection and the last positive HRV result or viral clearance (Fig. 3) . Notably, at the time of infection resolution in patients with HRV-PI, the number of T-CD4 +, T-CD8 + and NK lymphocytes was comparable with that in patients with HRV-AI (T-CD4 + 115 vs 95, T-CD8 + 371 vs 285, NK 220 vs 301 cells/l; p > 0.05). 

HRV infections are self-limiting in both pediatric and adult immunocompetent individuals, typically lasting <2 weeks [5, [23] [24] [25] . Moreover, sequential HRV infections caused by different HRV genotypes have been described as a common event in healthy subjects [3, 5, 23, 24, 26] . In contrast, HRV-PIs are infrequent events and have been reported only in immunocompromised patients such as lung transplant [14] , HSCT recipients [13, 15] , or patients with hypogammaglobulinaemia [24, 27] . Interestingly, HRV-PIs were associated with the prolonged shedding of a single HRV genotype suggesting the lack of efficient immunologic control of an initial HRV infection event. In the present retrospective study, HRV-PIs (≥30 days) were observed in eight pediatric HSCT recipients representing a small fraction of all HSCT recipients [1.5%]. In agreement with previous observations [15, 16, 23, 26] , also in our study, HRV-PIs were sustained by a single HRV genotype in all patients. The distribution of HRV species in HRV-PI and HRV-AI in this study reflects the worldwide HRV circulation, with HRV-A and HRV-C as the more prevalent HRV species observed [4] [5] [6] [7] .

In this and previous reports, where HRV-PIs have all been recorded in immunocompromised patients [13, 15, 16, 28] the role of B-or T-cell immunity in controlling HRV-PI is unclear. Peltola et al. recently documented that intravenous immunoglobulin therapy does not effectively correct the defective clearance of HRV in patients with primary hypogammaglobulinaemia [24] . In accordance, our results demonstrate the presence of HRV-PI in HSCT recipients receiving intravenous immunoglobulin replacement therapy, thus suggesting that impaired T cell-mediated immunity may have contributed to delayed HRV clearance. Similarly, Gooskens et al. reported that impaired T cell-mediated immunity correlated with delayed elimination of the influenza virus in HSCT recipients [29] . In the present study, HSCT recipients with HRV-PI had absent or very low T-CD4 + , T-CD8 + and NK lymphocyte counts during induction and before transplant engraftment, moreover T-CD4 + , T-CD8 + and NK lymphocyte counts at the time of infection onset were significantly lower than those observed in HSCT recipients with HRV-AI. T-CD4 + , T-CD8 + and NK lymphocyte counts were also significantly lower at the onset of infection with respect to the time of HRV clearance in patients with HRV-PI, and inversely correlated with HRV load. These findings also suggest that the peak HRV load at the onset of infection is an independent factor with respect to the immunological status of the patient. Finally, there was no significant difference in the T-CD4 + , T-CD8 + and NK lymphocyte counts between HSCT recipients with HRV-AI and those with HRV-PI at the time of infection resolution. This finding suggests that the reconstitution of cell-mediated immunity might be crucial for complete virus clearance. Nevertheless, our study has some limitations, including the absence of virus-specific immunological analyses, the small number of cases studied and the retrospective nature of design. As HRV-PIs are infrequent events, further investigations on large cohorts are needed to clarify the respective role of innate, T cell-mediated and humoral immunity in the clearance of HRV infections.

Although HRV is one of the most frequent viruses detected in severe respiratory syndromes in HSCT recipients [12, 14] , many aspects of HRV respiratory infections in patients with hematologic diseases still remain to be defined. In a recent study involving adult HSCT recipients, HRV was recognized as a significant cause of pneumonia [10] . Of note, in this study, an adult patient acquiring HRV infection during pre-transplant conditioning had persistent URTI and HRV positivity (3 months) [10] . This is consistent with our finding that in five HSCT recipients, HRV infection was acquired in the pre-transplant period; these subjects suffered only from mild URTI with no major clinical complications. However, in pediatric HSCT recipients the risk for development of LRTI cannot be excluded.

In conclusion, our data suggest that cellular immunity may play a role in the clearance of HRV infection. In addition, we demonstrated that persistence of HRV infection was due to unrestricted replication of a single virus strain, rather than sequential reinfections by different strains.

The authors of this manuscript have no conflicts of interest to disclose. 

This retrospective study was performed according to the guidelines of the Institutional Review Board on the use of biological specimens for scientific purposes in keeping with Italian law (art.13 D.Lgs 196/2003).

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