key: cord-0883551-rtlijgv5
authors: Ayona, Diyoly; Zarza, Sandra Madariaga; Landemarre, Ludovic; Roubinet, Benoît; Decloquement, Philippe; Raoult, Didier; Fournier, Pierre-Edouard; Desnues, Benoit
title: Human galectin-1 and galectin-3 promote Tropheryma whipplei infection
date: 2021-02-12
journal: Gut microbes
DOI: 10.1080/19490976.2021.1884515
sha: b01c47eee8abdbb00786c1cb94f7610153f57b49
doc_id: 883551
cord_uid: rtlijgv5

Tropheryma whipplei, is an actinobacterium that causes different infections in humans, including Whipple’s disease. The bacterium infects and replicates in macrophages, leading to a Th2-biased immune response. Previous studies have shown that T. whipplei harbors complex surface glycoproteins with evidence of sialylation. However, the exact contribution of these glycoproteins for infection and survival remains obscure. To address this, we characterized the bacterial glycoprofile and evaluated the involvement of human β-galactoside-binding lectins, Galectin-1 (Gal-1) and Galectin-3 (Gal-3) which are highly expressed by macrophages as receptors for bacterial glycans. Tropheryma whipplei glycoproteins harbor different sugars including glucose, mannose, fucose, β-galactose and sialic acid. Mass spectrometry identification revealed that these glycoproteins were membrane- and virulence-associated glycoproteins. Most of these glycoproteins are highly sialylated and N-glycosylated while some of them are rich in poly-N-acetyllactosamine (Poly-LAcNAc) and bind Gal-1 and Gal-3. In vitro, T. whipplei modulates the expression and cellular distribution of Gal-1 and Gal-3. Although both galectins promote T. whipplei infection by enhancing bacterial cell entry, only Gal-3 is required for optimal bacterial uptake. Finally, we found that serum levels of Gal-1 and Gal-3 were altered in patients with T. whipplei infections as compared to healthy individuals, suggesting that galectins are also involved in vivo. Among T. whipplei membrane-associated proteins, poly-LacNAc rich-glycoproteins promote infection through interaction with galectins. T. whipplei modulates the expression of Gal-1 and Gal-3 both in vitro and in vivo. Drugs interfering with galectin–glycan interactions may provide new avenues for the treatment and diagnosis of T. whipplei infections.

Tropheryma whipplei is the causative agent of Whipple's disease (WD), 1 a rare systemic illness that mainly affects middle-aged Caucasian men. 2 Classically, the disease occurs with severe infection of the small intestinal lining 3 which is characterized by the infiltration in the lamina propria by heavily infected large foamy macrophages strongly stained by the Periodic acid Schiff (PAS) reagent. 4 Later the infection may spread into different organs, mostly eyes, central nervous system and heart. 5,6 WD can be fatal without early and proper treatment. 7 The typical characteristics of the disease are weight loss, diarrhea, arthralgia, fever and hyper pigmentation of the skin 2 and fat accumulation in the small intestine and mesenteric lymph nodes. 8 T. whipplei infections can also manifest as chronic localized infections, such as endocarditis and encephalitis. 9, 10 Beside chronic infections, the bacteria can also cause acute infections mainly, gastroenteritis, pneumonia, and bacteremia. 5 Most individuals develop a protective immune response against T. whipplei infection 11, 12 and asymptomatic carriage of T. whipplei is common. 13 The development of the disease in a minor fraction of the population probably arise from a subtle genetic predisposition or an immune defect. 5 Indeed, in patients, impaired bactericidal activity toward T. whipplei is associated with alternative activation of phagocytic cells and an anti-inflammatory response, suggesting a defect in macrophage functions during WD. 11, 12, 14 The bacterium has a peculiar trilamellar membrane which is absent in other Gram positive bacteria. 2, 4 T. whipplei is the only known reduced genome (<1Mbp) bacteria in the class of Actinobacteria. 15 Its membrane contains polysaccharides with N-linked glycosylation and sialylation making the bacteria positive for PAS staining. 16 Among those membrane-associated glycoproteins, a WiSP (Whipplei Surface Proteins) protein called GpTw110 with an apparent molecular weight of 110 kDa has been identified. 16 Electron microscopy images of T. whipplei cultured in HEL cells show that bacteria can form both intracellular and extracellular biofilms. 4 Interestingly, the bacteria lose their glycosylation with prolonged culture, and this is associated with impaired replication in macrophages. In addition, GpTw110 is the main antigen recognized by immunoglobulins. Of note, serum samples from patients have a lower serologic response compared to asymptomatic carriers to T. whipplei and deglycosylated bacterial glycoproteins hinder the immune reactivity. Therefore, T. whipplei glycoproteins are believed to play an important role during bacterial replication and for immune evasion. 16 Galectins are a family of carbohydrate-binding proteins (also known as S-type lectins) which are found in vertebrates, in some invertebrates but also in microorganisms. In humans, galectins have a broad spectrum of cellular and pathophysiological functions, 17, 18 primarily mediated by carbohydrate recognition/interaction. 19 Members of this family are characterized by their distinct and evolutionary conserved carbohydrate recognition domain (CRD) which binds β-galactosides. 17 Generally galectins have a higher affinity for complex N-glycans and poly-N-acetyllactosamine (poly-LacNAc). However, each member of the galectin family has a unique glycan affinity spectrum. 20 Based on structural differences, galectins are categorized into the proto-, tandem-repeat and chimera-types. Proto-and chimera-type galectins have one CRD while tandemrepeat galectins have two distinct CRDs. Proto-type and tandem repeat galectins can non-covalently homodimerize and chimera-type form oligomers. 17 In addition, mammalian galectins can be found both intracellularly in the cytosol or associated with cellular organelles, or extracellularly, remaining free soluble molecules or associated with cell membrane and extracellular matrices. 18, 21 Thereby, galectins bind not only intrinsic glycans but also extrinsic glycans (e.g. glycans on pathogens). 22 Among different galectins, proto-type Gal-1 (14.5 kDa) and chimera-type Gal-3 (26 kDa) are highly expressed and secreted by immune cells, particularly by myeloid cells for which they are crucial for mediating immune cell migration, proliferation, adhesion and signaling. 23 Hence, these two galectins are considered as key regulators of immune responses. [24] [25] [26] [27] [28] During infections, Gal-1 and Gal-3 mediate immune responses primarily by interacting with glycans from pathogen and/or host glycans. Thereby, they can interfere with pathogen adherence and cell entry (e.g. Dengue virus), 29 act as pattern recognition receptors (PRRs) exerting antimicrobicidal effects (e.g. Candida spp.), 30 or inhibit microbial growth (e.g. Streptococcus pneumonia). 31 However, some pathogens have evolved strategies to utilize host galectins to enhance host cell entry and/or to subvert immune responses. 32 For example, Gal-1 enhances adherence of HIV-1 and Chlamydia trachomatis to their target cells by bridging glycans on host cell, 33, 34 while for Trichomonas vaginalis, Gal-1 not only enhances the adherence but also subverts immune responses in favor of the pathogen. 35, 36 Similarly, Gal-3 interaction with glycans expressed on Trypanosoma cruzi favors parasite attachment to target cells as well as immune evasion. 37 The involvement of T. whipplei glycoproteins in infection is far from understood. Therefore, in this study we aimed at investigating the role of galectinglycan interaction in T. whipplei infection. We found that both Gal-1 and Gal-3 bind poly-LacNAc-rich bacterial glycoproteins and enhance bacterial cell entry and that Gal-3 deficiency significantly reduces T. whipplei uptake by macrophages. Moreover, we found that sera from patients with T. whipplei infection have increased and decreased level of Gal-1 and -3, respectively, as compared with healthy controls. Thereby, we identified that T. whipplei utilizes its glycans to facilitate the infection via Gal-1-and Gal-3-mediated interaction.

In a first set of experiments, we performed PAS staining on bacterial whole cell lysate subjected to SDS/PAGE. As previously observed, 4 we found that bacteria harbor several PAS-positive glycoproteins that we further identified by mass spectrometry as 5 WiSPs, chaperone protein DnaK (Heat-shock protein 70), elongation factor Tu (EF-Tu), zinc-type alcohol dehydrogenase and sugar ABC transporter substrate-binding protein (L-arabinose/D-xylose) ( Figure 1a, Table S1 ). We next characterized the glycoprofile of T. whipplei by analyzing its interaction with several lectins using two different methods, lectin microarray (Fig. S1 ) and lectin blotting (Figure 1b) . Results indicated that T. whipplei glycoproteins harbor different types of glycans including glucose, mannose, fucose, N-acetylglucosamine (GlcNAc) and β-galactose. Bacterial whole-cell protein reactivity with different lectins indicated the presence of these glycans as follows: Concanavalin A (ConA) for α-mannose/α-glucose binding and N-glycans recognition (Figure 1b and Fig. S1 ), Burkholderia cenocepacia lectin A (BC2LA) for mannose-specific glycans, Pseudomonas aeruginosa I lectin (PA-IL) and Ulex europaeus agglutinin I (UEA-I) for fucose glycans, Griffonia simplicifolia lectin II (GSL-II) for GlcNAc (Fig. S1) , peanut agglutinin (PNA) for β-galactose (Figure 1b and  Fig. S1 ) and tomato lectin (TL) for poly-LacNAc and GlcNAc (Figure 1b) . In addition, a strong reactivity was observed for Trichosanthes japonica agglutinin I (TJA-1) (Fig. S1 ) and Sambucus nigra agglutinin (SNA) lectins (Figure 1b and Fig. S1 ), suggesting the presence of sialic acids moieties. Several bacterial proteins strongly interacted with SNA, including all PAS-stained glycoproteins as well as bands within the ranges of 95-55 kDa (Figure 1a , b). ConA strongly reacted with 4 major PAS-positive bands between 130 and 72 kDa and low molecular weight (LMW) bands below 55 kDa (Figure 1a ). Blotting T. whipplei whole cell lysate with PNA and TL resulted in almost the same binding pattern, suggesting the presence of β-galactose as poly-LacNAcs ( Figure 1a) .

Finally, we found that both Gal-1 and Gal-3 bind at least 8 glycoproteins (Figure 1b) , among which some corresponded to the glycoproteins that we identified by mass spectrometry, including the ~110 kDa WiSP (which represents the previously known as GpTw110), the chaperone protein DnaK, EF-Tu, zinc-type alcohol dehydrogenase and sugar ABC transporter substrate-binding protein (L-arabinose/D-xylose). However, Gal-3 appeared to have a higher reactivity than Gal-1 against the chaperone protein DnaK. These data suggest that T. whipplei harbors sialylated, N-glycosylated and poly-LacNAc-rich glycoproteins. The β-galactosedependent binding of Gal-1 ( Figure 1c ) and Gal-3 ( Figure 1d ) was further confirmed by lactose competitive binding assay. We found that two lactose washes were required to completely remove bound Gal-1 from bacteria, while only one was necessary for Gal-3. In addition, we were able to detect released Gal-3 in the elute fraction from the first lactose washing (Figure 1d ) but released Gal-1 from the first lactose washing was untraceable ( Figure  1c ). Hence, this result confirmed that Gal-1 and Gal-3 binding to T. whipplei depends on βgalactose.

Based on the role of Gal-1 and Gal-3 in immune response, we next aimed at defining the significance of T. whipplei interaction with these galectins. Macrophages were incubated with T. whipplei for 24 h and changes in Gal-1 and Gal-3 expression were assessed by immunoblotting. We found that when cells were infected with 200 bacteria per cell, expression of Gal-1 was increased and that of Gal-3 was decreased ( Figure 2a ). These changes in protein expression were further quantified by densitometry and were found significant compared to the non-infected control (Figure 2b , C). We next performed immunofluorescence staining to examine the interaction between T. whipplei and galectins. Confocal microscopy analysis of infected and non-infected cells demonstrated that T. whipplei drastically affect the cellular distribution of both Gal-1 and Gal-3 after 1 h or 24 h of infection (Figure 2d , E). Gal-1 and Gal-3 not only co-localized with bacteria but also accumulated at infected areas, disturbing the uniform cellular distribution. The Z-sections analysis of infected cells further confirmed the significant association of bacteria with membrane and cytosolic Gal-1 and Gal-3 and recruitment of Gal-1 (See also Fig. S2 Movie S1) and Gal-3 to bacteria (See also Fig.  S3 and Movie S2). Overall, these data revealed that T. whipplei modulates Gal-1 and Gal-3 expression, binds Gal-1 and Gal-3 and alters their cellular distribution.

As galectins may be involved during bacterial internalization, 34, 37 we wondered whether extracellular recombinant soluble Gal-1 and Gal-3 

GpTw110, actin, Gal-1 and Gal-3 immunoblotting of whole cell protein lysates obtained from macrophages exposed to 50 and 200 bacteria per cell for 24 h along with non-infected control. Band intensity were normalized to actin by densitometry analysis of Gal-1 (b) and Gal-3 (c). In B and C densitometry analysis represent the mean value of four independent experiments and bars represent the mean ± SEM. *P < .05, by unpaired-T test relative to the uninfected control. (d and e) Confocal microscopy of subcellular Gal-1 (d) and

Gal-3 (e) distribution upon infection, respectively. Immunofluorescence images represent macrophages exposed or not to T. whipplei for 1 h (Top) and 24 h (Bottom). Gal-1 and Gal-3 were labeled with anti-rabbit Gal-1 and anti-rabbit-Gal-3, respectively, followed by a goat anti-rabbit Alexa488-labeled IgG (green). T. whipplei was detected with anti-T. whipplei antibody followed by anti-mouse Alexa555-labeled IgG (Orange) and DNA was stained with DAPI (Blue). Co-localization of bacteria with Gal-1/-3 is indicated by yellow color.

would affect phagocytosis of T. whipplei. For this purpose, macrophages were infected in the presence of increasing concentrations of recombinant Gal-1 and Gal-3 for 3 h. We found that addition of both Gal-1 and Gal-3 significantly increase the intracellular bacterial load ( Figure  3 A, B) , till the cells reach a maximum cell infection, which was achieved with 16 ng/ml ( Figure 3a ) and 1.2-2 ng/ml (Figure 3b ) of Gal-1 and Gal-3, respectively. The role of Gal-1 and Gal-3 in bacterial uptake was further confirmed by two different approaches. First, we used competitive experiments in which lactose was used as the competitive molecule for other ligands that bind galectins. 38 Interestingly, we found that bacterial uptake was reduced by ~50% when cells were pre-incubated with 200 mM of lactose but not with 200 mM sucrose which was used as the negative control ( Figure  3c ). Second, we evaluated the bacterial uptake by Gal-1-and Gal-3-knock-out (KO) bone-marrowderived macrophages (BMDMs). Our results indicated that bacterial uptake was significantly reduced in Gal-3 KO cells compared to both WT type and Gal-1 KO cells, although, Gal-1 KO cells also showed a slight decrease in bacterial uptake compared to WT cells, which was not significant (Figure 3d ). Altogether, these results indicated that Gal-1 and/or Gal-3 interaction with bacterial glycans is critical for T. whipplei uptake by macrophages and that Gal-3 is required for efficient bacterial internalization. whipplei DNA copy number was determined by qPCR using T. whipplei-specific primers. Bars represent the mean ± SEM of 4 independent experiments. In A and B, **P < .01 and ***P < .001 by unpaired T test relative to the control. In C and D, *P < .05 and ***P < .001 by Mann Whitney U test relative to the control.

Finally, we investigated Gal-1 and Gal-3 levels in sera from healthy individuals and patients with T. whipplei infections (Figure 4 ). We found that Gal-1 levels were significantly higher in patients than in healthy controls with a median value of 119.9 ng/ml (range 68.20-453.8 ng/ml) in patients versus 65.03 ng/ml (range 39.60-79.44 ng/ml) in controls (Figure 4a ). In contrast, Gal-3 levels were significantly lower in patients than in healthy controls, with a median value of 2.73 ng/mL (range 1. 

Activities of galectins during infection are primarily mediated through binding to glycans from pathogen and/or host. Gal-1 and Gal-3 are the most abundant galectins expressed by immune cells. Emerging evidences emphasize that in some cases, these galectinglycan interactions may benefit the pathogen by providing the ability to adhere, by enhancing uptake as well as by modulating immune effector functions. 32 T. whipplei is an intracellular bacterium which replicates in alternatively activated macrophages. Although, it has been shown that T. whipplei glycoproteins are important for bacterial replication in macrophages, their contribution during the infection process is not well understood.

In this report, we aimed at evaluating the interaction of Gal-1 and Gal-3 with T. whipplei glycoproteins and their involvement during T. whipplei infection. By using lectin microarray and lectin blotting, we showed that T. whipplei harbors glucose, mannose, fucose, galactose and sialic acid, and expresses several poly-LacNAc-rich glycoproteins which bind both Gal-1 and Gal-3. Some of these glycoproteins were subsequently identified by mass spectrometry. As expected, the identified glycoproteins have previously been reported as membrane-associated in both Gram-positive and Gram-negative bacteria. For example, EF-Tu, which controls several virulence-associated functions, reaches cell surface to interact with host extracellular matrix components such as fibronectin to facilitate adhesion. 39 Similarly, the chaperone protein DnaK (Heatshock protein 70), which plays a critical role in overcoming stress responses is associated with or in close proximity to the membrane. 40, 41 Interestingly, T. whipplei DnaK seems to play a significant role. Indeed, T. whipplei up-regulates the expression of DnaK upon heat shock 42 and patients with classical Whipple's disease have reduced peripheral T-cell reactivity against DnaK compared to that of healthy controls. 43 Finally, and not surprisingly, we also identified WiSP as membrane-associated glycoproteins that bind galectins. Although their role during T. whipplei infection has not been clearly characterized, it was shown that prolonged axenic growth was associated with reduced WiSP glycosylation and impaired intracellular replication in macrophages. 16 data showed that most of the glycoproteins that we identified bind both Gal-1 and Gal-3, suggesting that galectins may contribute to the pathogenicity of T. whipplei through the interaction with these membrane-and virulence-associated glycoproteins.

TL binding as well as Gal-1 and Gal-3 binding to these glycoproteins strongly suggest the presence of poly-LacNac. Indeed, both Gal-1 and Gal-3 preferentially bind poly-LacNAc-rich glycans, but the affinity of Gal-3 is higher than Gal-1. For example, the dissociation constant (K d ) of Gal-1 and Gal-3 for LacNAc oligomers having repeating numbers of 5 are 39 μM and 0.19 μM, respectively. 20 However, SNA lectin binding also suggests that, in the meantime, most of these glycoproteins are sialylated. Several studies have shown that α-2, 6-sialylation inhibits galectin binding, except Gal-3 which binds internal LacNAc residues. 44 As a result, α-2, 6-sialylation of terminal LacNAc residues from T. whipplei glycoproteins may limit Gal-1 binding, but not that of Gal-3 and may explain the reduced band intensity when whole-cell lysates were probed with Gal-1 as compared with Gal-3. In addition, although Gal-1 and Gal-3 have a higher affinity for complex N-glycans (K d <8 μM), 20 we did not observe extensive binding of Gal-1 or Gal-3 to N-glycosylated proteins. This could also be a consequence of sialylation of glycoproteins. Overall when comparing the binding pattern of SNA and ConA, it seems that T. whipplei high molecular weight (HMW) proteins (>55 kDa) are associated with sialylation while LMW proteins are associated with N-glycosylation. Finally, we confirmed that binding of Gal-1 and Gal-3 to bacterial glycoproteins was dependent on β-galactose since lactose was able to completely displace bound galectin from bacteria. Nevertheless, two consecutive lactose washings were required for complete removal of bound Gal-1 from bacteria while one was sufficient for Gal-3. This observation may be the consequence of the stronger affinity of Gal-1 for poly-LacNAc chains while Gal-3 forms multiple low-affinity interactions with internal LacNAc residues. 44 We next showed that T. whipplei affects Gal-1 and Gal-3 expression and also modulates subcellular distribution of Gal-1 and Gal-3. Indeed, we found that upon infection, Gal-1 expression was increased while that of Gal-3 was decreased. In addition, T. whipplei disturbs the homogeneous distribution of Gal-1 and Gal-3 in macrophages and both Gal-1 and Gal-3 accumulate at infected areas of the cell where they co-localize with T. whipplei. Similarly, during C. trachomatis infection of HeLa cells the expression of Gal-1 is increased and Gal-1 accumulates nearby bacteria disturbing the homogenous cellular distribution of Gal-1. 34 Such results convince the ability of pathogens to modulate galectin expression as well as the ability of galectins to act as PRRs for pathogens.

Several studies have demonstrated that external addition of soluble Gal-1 and Gal-3 either enhance or inhibit cell entry of some pathogens. 32 For example, soluble Gal-1 enhances C. trachomatis uptake in a dose-dependent manner in vitro 34 while for T. cruzi, cell adhesion is increased with the addition of soluble Gal-3. 37 In contrast, addition of Gal-1 inhibits the internalization of Dengue virus and Nipah Virus. 29, 45, 46 We demonstrated that both Gal-1 and Gal-3 enhance T. whipplei phagocytosis by macrophages in a dose-dependent manner. Interestingly, addition of recombinant human Gal-3 as low as 0.2 ng/ml was sufficient to double the uptake of T. whipplei while with recombinant human Gal-1, this was achieved with 4 ng/ml. In line with this, inhibition of galectin binding by lactose drastically decreased bacterial phagocytosis. Even though both soluble Gal-1 and Gal-3 enhance the phagocytosis of T. whipplei by macrophages, the difference in bacterial uptake by Gal-1 KO and Gal-3 KO cells revealed that only Gal-3 is critical for optimal bacterial uptake. Therefore, our results suggest that both galectins are important for bacterial adherence through glycan-galectin-mediated interaction and that Gal-3 is required for cell entry .

Different studies have addressed the regulation of Gal-1 and Gal-3 levels in sera from rheumatoid arthritis (RA) patients. According to these studies, RA patients have higher Gal-1 as well as Gal-3 levels in sera compared to healthy individuals. [47] [48] [49] T. whipplei infections are commonly associated with joint manifestations mimicking seronegative RA. 50, 51 We found that expression of Gal-1 and Gal-3 was significantly higher and lower, respectively, in sera from patients with T. whipplei infection, as compared with healthy controls. Interestingly, in our study, 5 patients had symptoms of arthritis and the highest Gal-1 levels were measured from a patient with severe peripheral arthralgia. Therefore, further studies on larger sample size are worthwhile to understand whether the changes in serum Gal-1 and Gal-3 upon T. whipplei infection are associated with severity of arthritis and arthralgia.

Moreover, we found that in vitro, T. whipplei increased the expression of Gal-1 by macrophages while that of Gal-3 was reduced. According to several different studies, it seems that Gal-1 is more associated with antiinflammatory functions 52-54 while Gal-3 is mainly associated with pro-inflammatory functions. 55, 56 Both Gal-1 and Gal-3 are critical determinants of macrophage polarization. However, their exact contribution for activation of different macrophage phenotypes is still controversial. 57-61 Interestingly, T. whipplei infection is associated with Th2-biased immune responses, including alternative activation/M2 polarization of macrophages. 11, 14 Compared to Gal-3, Gal-1 expression is higher after in vitro infection but also in the serum from patients. In addition, the optimal soluble Gal-1 concentration that increases phagocytosis in vitro is also higher than that of Gal-3. However, we found that only Gal-3 is critical as a cell entry mediator/receptor. It is therefore possible that at the beginning of the infection, both Gal-1 and Gal-3 enhance cell entry, and at later stages, optimal Gal-1 and Gal-3 levels are reached both locally and systemically to shape the T. whippleifavorable Th-2 dominant microenvironment, although other factors are probably involved. Hence, circulating Gal-1 and Gal-3 levels may thus provide a snapshot of the polarization status of the immune response against T. whipplei infection.

Finally, several single nucleotide polymorphisms (SNPs) of Gal-1 and Gal-3 have been associated with increased or decreased Gal-1 and Gal-3 expression in sera. 62, 63 Such SNPs may also affect the uptake of the pathogen. Indeed, during enterovirus EV71 infection, a nonsynonymous Gal-3 genetic variant is associated with lower virus titer than the wild-type allele. 62 Similarly, it has been reported that susceptibility to influenza A (H7N9) infection is associated with functional variants of Gal-1 which affect its expression. 63 When considering the opsonin effect of both Gal-1 and Gal-3 during T. whipplei infection and the spectrum of T. whipplei infections, it is worthwhile to assess the association between the type of the infection (carriage, chronic isolated or chronic systemic) and the polymorphism of Gal-1 and Gal-3. Finally, the drugs which are used to treat T. whipplei infection, doxycycline and hydroxychloroquine have been identified as chemical modulators of Gal-1 and Gal-3 expression in humans and their role against infection (e.g. Coronaviruses) or cancer may also arise from galectin inhibition. [64] [65] [66] [67] In conclusion, our study demonstrates that both Gal-1 and Gal-3 favor T. whipplei infection by enhancing the internalization of bacteria through glycan-galectin-mediated interaction, but only Gal-3 is required for optimal bacterial uptake. Bacteria also modulate the expression and distribution of Gal-1 and Gal-3. In addition, the enhanced Gal-1 and decreased Gal-3 levels in patients with T. whipplei infection may reflect galectin-mediated immune evasion by T. whipplei. Further studies are required to determine the involvement of specific T. whipplei glycoproteins in galectin recruitment and immune evasion. Therefore, the understanding of galectin-glycan interaction and the interplay of different galectins during T. whipplei infections may be of critical importance for the development of novel therapeutic and diagnostic approaches.

Tropheryma whipplei str. Twist was cultured in axenic medium as previously described. 68 THP-1 (ATCC® TIB-202) cells were obtained from the American Type Culture Collection. Cells were maintained in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Gibco) at 37°C and 5% CO 2 . Cells were kept at a minimum density of 3 × 10 5 cells/ml and were passaged when reaching 8 × 10 5 cells/ml. Cells were sub-cultured in 24 well plates or 6 well plates at a cell density of 5 × 10 5 and 1 × 10 6 cells/well, respectively. Phorbol-12myristate 13-acetate (PMA) (Sigma-Aldrich) was added to a final concentration of 100 nM to differentiate THP-1 cells into macrophages. After 3 days, cells were gently washed once with 1X phosphatebuffered saline (PBS, Gibco) and incubated for an additional 24 hours in 10% FBS in RPMI 1640. To evaluate the bacterial cell entry in the presence of added recombinant Gal-1 and recombinant Gal-3, cells were infected in the presence of indicated recombinant Gal-1 or recombinant Gal-3 and incubated for 3 hours. To evaluate the impact of blocking galectin on bacterial cell entry, macrophages were pre-incubated 2 hours with and without 200 mM lactose or 200 mM sucrose and thereafter, cells were infected for 2 hours. To evaluate the Gal-1 and Gal-3 expression and cellular distribution cells were infected for 1 hour or 24 hours. Bone marrow-derived macrophages (BMDMs) from Gal-1 KO (kindly given by Dr. Stéphane Mancini, Cancer Research Center of Marseille, France), Gal-3 KO (kindly given by Dr. Ludger Johannes, Institut Curie, France) and C57BL/6 wild-type (WT, Charles River laboratories) mice were generated as previously described. 69 Unless notified, macrophages were infected with 50 bacteria per cell and incubated 37°C and 5% CO 2. At the end of each infection period, cells were washed twice with PBS to remove free bacteria.

Bacteria (1 × 10 9 ) were sonicated for 2 minutes at 20 W in TS-lysis buffer (7 M urea, 2 M Thiourea, 1% CHAPS). Protein quantity of bacterial cell lysate was determined by Bradford Assay (Bio-Rad). Amount of 40 µg from bacterial whole cell lysate was resolved on 10% SDS/PAGE. After migration, gel was incubated in 12.5% tri-chloro acetic acid for 30 minutes, rinsed with distilled water for 15 minutes and incubated for 50 minutes in 1% Periodic acid in 3% acetic acid. Excess periodic acid was removed by several washings with distilled water and the gel was incubated in distilled water at room temperature overnight. Schiff's reagent (Sigma-Aldrich) was then added in the dark for 50 minutes and subsequently washed the gel with 0.5% metabisulfite for 10 minutes, trice. After removing excess stain by several washings with distilled water, the gel was reserved in 5% acetic acid and scanned.

Protein bands were cut from PAS-stained gel. Separated bands were washed several times with acetonitrile and water and digested overnight at room temperature into peptides using in-gel digestion with proteomics grade trypsin (Agilent Technologies). The peptides were extracted from the gel using acetonitrile. A matrix-assisted laser desorption/ionization time-of-flight spectrometry (MALDI-TOF-MS) on a Bruker Autoflex Speed spectrometer (Bruker Daltonics, Wissembourg, France) was used to identify the protein bands. The mass spectrometer was calibrated externally using Bovine serum albumin tryptic peptides. Peptide mixture (1 μL) was co-crystallized onto the anchorchip MALDI-TOF target plate with an equal amount of matrix solution (0.3 mg/mL of αcyano-4-hydroxycinnamic acid in acetone and ethanol in 1:2 volume ratio and 0.1% trifluoroacetic acid). Mascot software was used for protein identification using peptide mass fingerprinting. Searches were performed against all available sequences in public databases.

Equal amounts of bacterial whole-cell lysate were resolved in 10% SDS/PAGE gel and transferred onto 0.45-µm nitrocellulose membranes (Bio-Rad). Membranes were incubated in carbo-free blocking buffer (Vector) for 1 h. For lectinblotting, strips were probed with the following biotinylated lectins (5 µg/ml) (Vectors) for 1 hour: Sambucus nigra (SNA), Lycopersicon esculentum (Tomato) lectin (TL), Peanut agglutinin (PNA) and Concanavalin A (ConA). For blotting with Gal-1 and Gal-3, strips were incubated for 2 hours with biotinylated recombinant Gal-1 and Gal-3 (1:1000) (Peprotech). Thereafter, strips were washed with 0.3% Tween-20 in PBS and incubated with streptavidin-HRP (1:10000) (Sigma-Aldrich) for 1 hour followed by washings with 0.3% Tween-20 in PBS. Finally, strips were treated with ECL Western blotting substrate (Promega) and visualized under Fusion FX (Vilber Lourmat).

Gal-1 and Gal-3 binding assay was performed as described previously. 34 Briefly, bacteria were incubated with recombinant Gal-1 or Gal-3 (0.3 µg/ml) for 1 hour at 4°C, washed with PBS to remove unbound galectin and washed with lactose twice (500 μl, 200 mM) to remove bound galectin to bacteria. Equal amount of bacterial protein lysates obtained from bacterial pellets incubated with recombinant galectins (TW), bacterial pellets incubated with recombinant galectins and washed once with lactose (TW1), elutes from TW1 bacterial pellets, bacterial pellets incubated with recombinant galectins and washed consecutively twice with lactose (TW2) were resolved in 15% SDS/PAGE gel and transferred onto 0.45-µm nitrocellulose membrane. Membrane was blocked in carbo-free blocking buffer for 1 hour and incubated overnight at 4° C with anti-Gal-1 (1:1,000) or anti-Gal-3 (1:1,000) followed by incubation with goat anti-rabbit HRPconjugated IgG (1:5000) (Invitrogen) for 1 hour. The T. whipplei-specific GpTw110 protein was used as loading control and labeled by incubating blots overnight at 4°C with GpTw110 specific antimouse polyclonal antibody (1:1000). 16 Blots were visualized using ECL Western blotting substrate under Fusion FX (Vilber Lourmat).

Cells were lysed with TS-lysis buffer and protein quantity was assessed by Bradford assay (Bio-Rad). Equal amount of protein lysates were loaded in 15% SDS/PAGE gel and proteins were transferred onto 0.45-µm nitrocellulose membranes. Membranes were incubated in carbo-free blocking buffer for 1 hour. To label Gal-1 and Gal-3 blots were incubated overnight at 4°C, respectively, with anti-Gal-1 (1:1,000) antibody and anti-Gal-3 (1:1,000) antibody followed by incubation with goat anti-rabbit HRPconjugated IgG (1:5000) for 1 hour. T. whippleispecific GpTw110 was used as the loading control for bacteria. Monoclonal Anti-β-Actin−Peroxidase antibody (1:25000) (Sigma-Aldrich) for 1 hour was used to label β-actin. After each incubation blots were washed in 0.3% Tween-20 PBS. Bands were visualized using ECL Western blotting substrate under Fusion FX (Vilber Lourmat).

Cells grown on coverslips were fixed in 3% paraformaldehyde for 15 minutes and permeabilized with Triton X-100 (0.1%) for 3 minutes. Antihuman Gal-1, anti-human Gal-3 (1:1000) and T. whipplei-specific mouse antibodies (1:2000) prepared as previously described (Liang, Z., 2002) were used to label Gal-1, Gal-3 and bacteria, respectively. Donkey Anti-Rabbit IgG (1:1000) (Alexa Fluor® 488, Invitrogen) and goat anti-mouse IGg (1:1000) (Alexa Fluor® 555, Invitrogen) were used as secondary antibodies for 45 minutes to label the two galectins and bacteria, respectively. Nuclei were labeled by with 4′,6-diamidino-2-phenylindole (1:500) for 15 minutes (DAPI, Invitrogen). After each labeling coverslips were washed several times with 5% FBS in PBS. Air-dried coverslips were mounted with mowiol and slides were observed using LSM 8000 Airyscan confocal microscope (Zeiss LSM 800) under oil immersion objective (63 × 1.5).

Cells were lysed with Triton X-100 (1% in PBS) and DNA was extracted using QIAamp DNA MiniKit (Qiagen). The quantity and the quality of extracted DNA was assessed by nanodrop spectrophotometer (Nanodrop technologies). Quantitative polymerase chain reaction (qPCR) was performed using specific primers for T. whipplei 16S-23S ribosomal intergenic spacer region as described previously 4 and Smart SYBR Green kit (Roche).Threshold cycles (Ct) were obtained using CFX Touch Real-Time PCR detection system (Bio-Rad). For each qPCR run, a standard curve was generated using a serial dilution ranging from 10 2 to 10 8 copies of the intergenic spacer region of T. whipplei.

A total of 7 patients with proven diagnosis of T. whipplei infection by a strategy developed in our institute 70 were included in this study: 7 Whipple's disease patients (6 Males; mean age 68 ± 10 years and 1 Female; age 65 years).The study was validated by the ethics of the Mediterranee Infection Institute under reference 2016-025. Serum samples from 9 healthy donors (5 Males; mean age 41.6 ± 9.4 years and 4 females; mean age 39.8 ± 2 years) were obtained from the Etablissement Français du Sang (Marseille, France) according the convention n°7828. Serum concentrations of Gal-1 and Gal-3 were determined by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer's instructions using immunoassays for Gal-1 from RayBiotech, Inc (Assay sensitivity 4.10 ng/mL) and for Gal-3 from Peprotech (Assay sensitivity <10 pg/mL).

Densitometry analysis of Western blots was performed using Image J 1.52a software. Statistical Analysis was performed using GraphPad Prism 6.0 Software. Data represent the mean ± SEM of N experiments. For simple unpaired analysis between two independent groups, unpaired T test was used. Mann-Whitney U test was used when the dependent variable is not normally distributed. P values less than 0.05 were considered as statistically significant.

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Peripheral T-Cell Reactivity to Heat Shock Protein 70 and Its Cofactor GrpE from Tropheryma whipplei Is Reduced in Patients with Classical Whipple's Disease

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Tropheryma whipplei and Whipple's disease

Authors were grateful to Dr. Bernard Henrissat for his helpful suggestions, Dr. Lea Luciani for providing clinical information of patients, and Dr. Stéphane Mancini and Dr. Ludger Johannes for providing Gal-1 KO and Gal-3 KO mice, respectively.

DA designed the study, performed experiments and wrote the manuscript, SMZ performed experiments, LD and BR performed experiments, PD performed experiments, DR funded the study, PEF supervised the study and provide critical comments to the manuscript and BD supervised the study and provide critical comments on the manuscript.

No potential conflicts of interest were disclosed.

The authors report no conflict of interest.

This work was supported by the French Government under the « Investissements d'avenir » (Investments for the Future) program managed by the Agence Nationale de la Recherche under Grant number 10-IAHU-03; by Région Provence Alpes Côte d'Azur and European funding FEDER PRIMI.

http://orcid.org/0000-0002-4874-3706