key: cord-0877124-f9elbore
authors: Zhang, Fengqin; Wei, Yanqiu; He, Li; Zhang, Huilan; Hu, Qiongjie; Yue, Huihui; He, Jianhan; Dai, Huaping
title: A trial of pirfenidone in hospitalized adult patients with severe coronavirus disease 2019
date: 2022-02-05
journal: Chin Med J (Engl)
DOI: 10.1097/cm9.0000000000001614
sha: 966f7dcc46af93db8dfd20488a320b0b417f5ea2
doc_id: 877124
cord_uid: f9elbore

nan

To the Editor: Coronavirus disease 2019 (COVID-19), which broke out in 2019, has become a global pandemic. Similar to severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003, SARS-CoV-2 could cause acute lung injury and cytokine storms characterized by the increased interleukin (IL)-8, IL-6, and tumor necrosis factor a (TNFa). [1] Perspective studies in those survivors from the severe acute respiratory syndrome (SARS) epidemic in 2003 revealed that those SARS patients manifested varying degrees of pulmonary interstitial fibrosis. [2] Similarly, patients with severe COVID-19 are also featured by the diffuse alveolar damage along with alveolar interstitial fibrosis. [3] Pirfenidone (Beijing Contini Pharmaceutical Co., Ltd, Beijing, China) can inhibit the biological activity of fibroblasts and reduce matrix collagen deposition, prevent inflammasome activation and limit oxidative stress responses, supporting a therapeutic potential against idiopathic pulmonary fibrosis (IPF). [4] Given the presence of alveolar interstitial fibrosis in severe COVID-19 patients and the effect of pirfenidone on anti-inflammatory responses and anti-fibrosis, we hypothesized that pirfenidone can play a positive role in COVID-19 patients, thereby reducing the incidence of complications following SARS-CoV-2 infection. We thus conducted a clinical trial to assess the potential therapeutic effect of pirfenidone on severe COVID-19 patients. of ambient air or partial oxygen pressure (PaO 2 ) to inhaled oxygen (FiO 2 ) of 300 mmHg or less, were eligible for the study. The exclusion criteria were: patient disinterest in the study; the presence of conditions that did not allow for safe compliance, including hypersensitivity to pirfenidone; liver disease (eg, alanine aminotransferase levels >5 times the upper limit of the normal range [ULN] or aspartate aminotransferase levels >5 times ULN; contraindications of pirfenidone and pre-existing interstitial lung disease [ILD]). Consecutive patients, who meet the inclusion criteria, were randomly assigned in a 1:1 ratio to pirfenidone (200 mg, three times daily for the first two days and 400 mg, three times daily thereafter) plus standard therapy or standard therapy alone. Pirfenidone was given through a nasogastric tube in patients who were unable to swallow.

The primary end-point was the absolute changes from baseline in the total score on the King's Brief Interstitial Lung Disease (K-BILD) questionnaire at the 4th week, a change between 4 and 8 points has been suggested to represent a meaningfulchange. Secondary endpoint was the absolute change in computed tomography (CT) values, the total CT value was the sum of individual lobe values and ranged from 0 (no involvement) to 25 (maximum involvement). Other secondary outcomes included clinical laboratory findings (cytokines, biochemical indicators, etc) and the proportion of patients with clinical improvement. Safety outcomes included adverse events that occurred during treatment and premature discontinuation of treatment. Adverse events were classified according to the National Cancer Institute Common Terminology Criteria for Adverse Events, Although the difference of K-BILD scores did not reach a statistical significance after treatment (75.93 ± 10.07 vs. 76.33 ± 9.15, P = 0.911), a trend for the increase from baseline in patients following a 4-week of pirfenidone treatment was noted as compared to that of patients assigned in the standard treatment group (DK-BILD, 26.53 ± 11.12 vs. 22.73 ± 8.00; 3.80 [95% confidence interval, CI = À4.87 to 12.47]) [Supplementary Table 2 , http://links.lww.com/CM9/A646]. Similarly, there was no significant difference between two groups in terms of CT images (P = 0.745) after a 4-week of treatment, but some score changes including consolidation (0.30 ± 0.65 vs. 1.07 ± 1.17, P = 0.007), GGO (À12.27 ± 5.72 vs. À11.57 ± 4.07; between-group difference = À0.70, 95% CI = À2.97 to 1.57), and reticulation (À0.90 ± 5.26 vs. À0.30 ± 6.98; between-group difference = À0.60, 95% CI = À3.40 to 2.20) were observed, which reflected the improvement of lung inflammation and interstitial changes [Supplementary Table 3 The levels of pulmonary inflammatory cytokines or coagulopathy biomarker from baseline to the 4th week after receiving treatment were significantly decreased in the pirfenidone group as compared to those from the standard care group, such as IL-2R (À299.00, 95% CI = À430.50 to À105.00, P = 0.010), TNF-a (À3.50, 95% CI = À5.00 to À0.10, P = 0.049), and D-Dimer (À4.57, 95% CI = À8.98 to À0.16, P = 0.021) [Supplementary The percentages of patients with any adverse event or serious adverse event were similar between the patients from both groups. Among patients with adverse events, 11% (8/73) of patients reduced the dose of pirfenidone and 3% (2/73) of patients discontinued. Among those eight patients with pirfenidone reduced-dose, four cases were reduced to 600 mg/day due to gastrointestinal discomfort and the remaining four cases were reduced to 600 mg/day due to rash. The most common adverse event was diarrhea, which was reported in 11 out of 73 (15%) patients from the pirfenidone group. Some patients have elevated alanine aminotransferase and alanine aminotransferase level Given that the COVID-19 is a type of infectious disease that could be transmitted through the respiratory tract, lung function test was not included in this study. According to previous studies, patients infected with SARS-CoV-2 are far more likely to form an interstitial change in the lung, while our statistical indicators including K-BILD and CT image ratings reflected improved situation in terms of interstitial changes in pirfenidone treated patients. However, we failed to observe a significant difference between the two groups both for K-BILD and CT scores. Since our observation period only lasted for 4 weeks, our trial did not yield a significantly positive result, and the observation time could be a major factor.

It was noted that pirfenidone did not improve fibrosis, but it did not make the disease worse. Nevertheless, pirfenidone did manifest a strong effect on mitigating the cytokine storm, which seems to be responsible for the complications in severe COVID-19 patients. Indeed, comparative analysis revealed that the levels of IL-2R and TNF-a were decreased significantly following pirfenidone administration. Although the anti-inflammatory effect has not been widely appreciated, pirfenidone has been shown to downregulate inflammatory pathways and the compound may have considerable potential to be deployed as a nonsteroidal anti-inflammatory agent. [5, 6] To our surprise, our study also found that pirfenidone could significantly decrease the level of D-Dimer, which is relevant to the coagulopathy in the blood. There is evidence that COVID-19 renders patients with an increased risk for acute pulmonary embolism, and anticoagulant therapy might be associated with improved outcomes in patients with severe COVID-19. [7] Therefore, Chinese Medical Journal 2022; 135(3) www.cmj.org treatment of COVID-19 with pirfenidone may have the potential to reduce the incidence of thrombosis complications.

The safety and side-effect profile of pirfenidone in patients with severe COVID-19 associated ILD was similar to that observed in patients with IPF. [8] Despite the presence of certain side effects, but they could be easily managed with supporting therapies and temporary dose reductions or discontinuation. No fatal events were reported in our study, confirming the good safety profile of this drug even in fragile patients.

Our study also has several limitations. First, our sample size is limited. Second, although we have tried to avoid bias as much as possible during the trial, while this possibility cannot be completely excluded. Third, our trial lacks of dynamic clinical and laboratory data such as immune cell subsets and so on. Finally, our trail only lasted a 4-week of observation time, which could be a factor to confirm the antifibrotic effect of pirfenidone. In addition, glucocorticoids could also be a potential factor influencing the results of the study, even though there was no significant difference in the initial dosage, total dosage, and duration of treatment between the two groups.

Although pirfenidone has not been found to significantly improve the interstitial changes in severe COVID-19 patients, the trial, however, confirmed the benefits of pirfenidone therapy in anti-inflammatory responses, and obtained feasible evidence supporting a potential benefit in anti-thrombotic complications. Collectively, our study found that pirfenidone can be considered as a viable drug to treat patients with severe COVID-19, and confirmed that pirfenidone possesses a good tolerability profile without safety alert.

This study was supported by grants from the SARS-CoV-2 Pneumonia Emergency Technology Public Relation Project of Tongji Medical College, Huazhong University of

Risk factors associated with acute respiratory distress syndrome and death in patients with coronavirus disease 2019 pneumonia in Wuhan, China

SARS: prognosis, outcome and sequelae

Histopathologic changes and SARS-CoV-2 immunostaining in the lung of a patient with COVID-19

Pirfenidone: molecular mechanisms and potential clinical applications in lung disease

Pirfenidone inhibits T-cell activation, proliferation, cytokine and chemokine production, and host alloresponses

Pirfenidone protects against paraquat-induced lung injury and fibrosis in mice by modulation of inflammation, oxidative stress, and gene expression

Anticoagulant treatment is associated with decreased mortality in severe coronavirus disease 2019 patients with coagulopathy

Double-blind, placebo-controlled trial of pirfenidone in patients with idiopathic pulmonary fibrosis

A trial of pirfenidone in hospitalized adult patients with severe coronavirus disease 2019

None.