key: cord-0876867-n5tvml4h authors: Barnabei, Remo; Di Michele, Giulio; Cellini, Antonio; Amicosante, Gianfranco; Perilli, Mariagrazia; Bellio, Pierangelo; Piccirilli, Alessandra; Celenza, Giuseppe title: Transient disappearance of CD19(+)/CD5(+) B‐lymphocyte clone in peripheral blood in a patient with CLL during SARS‐CoV‐2‐related mild disease date: 2021-06-22 journal: Clin Case Rep DOI: 10.1002/ccr3.4238 sha: 37bb9e11b6a4bb933de3b054747b8047c1b0f12a doc_id: 876867 cord_uid: n5tvml4h Although lymphopenia is currently considered a good predictor for the prognosis of COVID‐19, it must be critically evaluated in patients with CLL, where other clinical markers should be considered to define the prognosis and treatment. BARNABEI Et Al. ACE2 inhibitor ramipril (2.5 mg) therapy and metformin (500 mg twice a day) glycemic control therapy for type II diabetes. Hematological parameters, at presentation, were consistent with the previous CLL diagnosis (leukocytes 18.33 × 10 3 /µL, comprising 56.5% lymphocytes and 40.1% neutrophils of the total white blood cell count) and biochemical alterations included: glycemia (145 mg/dL), C-reactive protein (18. 73 mg/dL), and an erythrocyte sedimentation rate of 120 mm/h. Estimated glomerular filtration rate, serum electrolyte levels, and cardiac, muscle, pancreatic, and liver enzyme levels were all within physiological limits, except for gamma-glutamyltransferase (72 UI/L), which was moderately above the upper physiological limit, and coagulative indices and plasma brain natriuretic peptide levels were also within physiological limits. NMR of the lumbosacral rachis was compatible with suspected spondylodiscitis, and chest Xrays did not demonstrate lung alterations. The patient was treated with levofloxacin (750 mg/d) and teicoplanin (400 mg/d) for 4 weeks and oral pregabalin 75 mg/d, oxycodone/naloxone 5/2.5 mg/d, and tramadol/ paracetamol 37.5/325 mg/d for pain relief. Thirteen days following admission, the patient's CRP levels had dropped to 0.7 mg/dL and, with unchanged hematological parameters, he was transferred from Internal Medicine to long-term care. Twenty-one days following admission, the patient was diagnosed as SARS-CoV-2 positive by real-time RT-PCR of a nasopharyngeal swab and was moved to the Infectious Diseases department. The patient was asymptomatic but exhibited an increase in CRP levels to 4.32 mg/dL and a decrease in leukocyte count to 5.79 × 10 3 /µL. Five days later (26 days from admission), the patient's arterial oxygen saturation (SaO 2 ) levels dropped to ≈90%, chest CT revealed prevalently peripheric bilateral ground-glass opacities, compatible with new coronavirus infection. Blood CRP and ferritin levels rapidly increased to reach maximums of 11.89 mg/dL and 1695.6 mg/dL, respectively, within 3 days, preceded by a pronounced reduction in leukocytes and lymphocytes numbers, during infection, to within the physiological range (30.6% neutrophils and 64.8%lymphocytes at admission to 58.5% neutrophils and 37.2% lymphocytes during SARS-CoV-2 infection; Figure 1 ). The patient was successfully treated with darunavir (800 mg/d), ritonavir (100 mg/d), and with hydroxychloroquine (200 mg twice per day) for 1 week, by which time his CRP and ferritin levels had returned to the physiological range, in the absence of other biochemical changes. Three months following infection, the patient was asymptomatic, exhibited biochemical and coagulative indices, and arterial blood gas tests within the physiological range and, despite F I G U R E 1 Aligned time-course plots of significative hematological parameters (leukocytes count and lymphocytes percentage) and inflammation markers (Creactive protein and ferritin) recorded from hospital admission. Dashed lines represent the upper limit of the reference ranges a marked anti-SARS-CoV-2 IgG humoral immune response, remained positive for SARS-CoV-2 for up to 3 months following the disappearance of symptoms. Between the thirty-fifth and seventy-first days, following COVID-19 diagnosis, the patient's mean (±SD) leukocytes count was 8.64 × 10 3 ± 0.36/µL and mean (±SD) neutrophil count was 3.09 × 10 3 ± 0.20/µL, associated with a moderate mean (±SD) lymphocytosis of 5.02 × 10 3 ± 0.46/µL), resulting in a typical differential mean (±SD) WBC of 58.13 ± 3.08% neutrophils and 35.86 ± 3.10% lymphocytes (Figure 1 ). In this case study, we report the biochemical, hematological, and coagulative changes in a COVID-19 patient presenting with stage 2 CLL, diabetes, and hypertension; three conditions reported negatively affect the clinical COVID outcome over the whole duration of SARS-CoV-2 infection. The mortality rate for COVID-19 depends on several factors and ranges between 2% and 5%. 5 Fatal outcomes for COVID-19 associate more frequently with comorbidities and/or immunosuppression, including hematological malignancies. 6 CLL is more prevalent in the elderly, is characterized by immunodeficiency, and often associates with comorbidities. Although relatively little is known about the clinical course of COVID-19 in CLL patients, estimates suggest that around 30-33% of CLL patients with symptomatic SARS-CoV-2 infection have a poor outcome and, in general, are considered to be more susceptible to severe disease. 6, 7, 8 The deregulation of adaptative immunity in CLL results in humoral immunodeficiency, even in the absence of disease progression, and is likely, therefore, to influence antibody responses. 9 Hypertension and diabetes represent comorbidities that are more frequently associated with a fatal outcome in COVID-19, which in Italy have been associated with 73.8% and 33.9% COVID-19 deaths, respectively (Characteristics of COVID-19 patients dying in Italy. Report based on available data on March 20th, 2020, National Institute of Health, https://www.epice ntro.iss.it/en/coron aviru s/sars-cov-2-analy sis-of-deaths). Notwithstanding these statistics and the complete absence of therapeutic recommendations for COVID-19 in patients with lymphoid malignancies, which became available only months later, 10,11 this particular CLL patient who also presented with diabetes and hypertension was efficaciously treated for COVID-19 in the empirical strategy described above. Forty-five days following COVID-19 diagnosis, the patient exhibited a strong IgG, but not IgM, anti-SARS-CoV-2 humoral immune response, attributable to the low titer at the time of analysis, a transient immune response not captured within the testing window and suboptimal seroconversion in CLL patients. 12 Nevertheless, this patient remained SARS-CoV-2 positive for 3 months following the disappearance of symptoms, suggesting that CLL-induced immunodeficiency might negatively affect viral clearance, despite the IgG seroconversion. Surprisingly, the leukocytosis detected at hospital admission, compatible with CLL, reduced dramatically during SARS-CoV-2 infection to within the physiological range and remained at this level for up to 3 months, and the CD19 + / CD5 + CLL clone that characterized this patient's disease disappeared, resulting in a physiological neutrophil-to-leukocyte ratio and transient remission of CLL. Lymphopenia and neutrophilia characterize COVID-19 in non-CLL patients and correlate with COVID-19 intensive care unit admissions, ARDS and disease severity. [13] [14] [15] [16] It is, therefore, possible that lymphopenia and neutrophilia induced by SARS-CoV-2 infection may explain the transient CLL remission exhibit by the patient in this report. In support of this hypothesis, Vardanyan and colleagues reported a case of a 61-year-old CLL patient (Binet stage A), who experienced a partial 6 days resolution of CLL lymphocytosis during SARS-CoV-2 infection 17 and, based on previous studies, 18 implicated a potential antiproliferative role for IL-6. Within this context, it is also worth noting that an antitumor immune response resulting from infection-induced inflammatory cytokine expression has been hypothesized in SARS-CoV-2-induced remission of Hodgkin lymphoma. 19 It is, therefore, conceivable that the proinflammatory response triggered by SARS-CoV-2 infection may have been responsible for the transient disappearance of the CD19 + /CD5 + CLL clone in this patient. This hypothesis is corroborated by the maintenance of white blood cell numbers within the physiological range for the duration of SARS-CoV-2 infection in this CLL patient. Based on the patient's clinical history and comorbidities (CLL, diabetes, and hypertension) associated with poor prognosis in COVID-19, the patient's optimum outcome in this case report was completely unexpected and cautions against directly generalizing from previous research finding should be taken. The distinctive kinetics and tropism patterns of SARS-CoV-2 in patients with underlying hematological malignancies call for specific clinical and diagnostic management strategies. Even though lymphopenia and associated neutrophilia are valuable prognostic markers and useful therapeutic efficacy indices, other biochemical markers must also be carefully considered in COVID-19 patients with hematological malignancies of the immune system. COVID-19 in a patient with chronic lymphocytic leukaemia Covid-19 infection in therapy-naive patients with B-cell chronic lymphocytic leukemia Chronic lymphocytic leukemia management in Italy during the Covid-19 pandemic: a campus CLL report Outcomes of Covid-19 in patients with CLL: a multicenter international experience Coronavirus disease 2019 (COVID-19) and immune-engaging cancer treatment Could coronavirus disease 2019 (Covid-19) render natural immunity to re-infections? A spotlight on the therapeutic pipeline Covid-19 severity and mortality in patients with chronic lymphocytic leukemia: a joint study by ERIC, the European Research Initiative on CLL, and CLL Campus Anti-SARS-CoV-2 antibody response in patients with chronic lymphocytic leukemia Perturbation of the normal immune system in patients with CLL How to manage lymphoid malignancies during novel 2019 coronavirus (CoVid-19) outbreak: a Brazilian task force recommendation Australian and New Zealand consensus statement on the management of lymphoma, chronic lymphocytic leukaemia and myeloma during the COVID-19 pandemic Unexpected kinetics of anti-SARS-CoV-2 total antibodies in two patients with chronic lymphocytic leukemia Lymphopenia is associated with severe coronavirus disease 2019 (COVID-19) infections: a systemic review and meta-analysis Lymphopenia predicted illness severity and recovery in patients with Covid-19: a single-center, retrospective study Lymphopenia during the Covid-19 infection: what it shows and what can be learned Lymphopenia and neutrophilia at admission predicts severity and mortality in patients with Covid-19: a meta-analysis Chronic lymphocytic leukaemia in Covid-19 The two faces of IL-6 in the tumor microenvironment SARS-CoV-2-induced remission of Hodgkin lymphoma