key: cord-0871935-5l06vvpy authors: Chen, Bingbing; Jin, Hui; Yang, Rong title: Application of fluorescence method in the process of personal protective equipment removal date: 2020-05-07 journal: Am J Infect Control DOI: 10.1016/j.ajic.2020.04.023 sha: 47fa7ff30abe009992966b79e4e385c04289c18c doc_id: 871935 cord_uid: 5l06vvpy nan Hangzhou center for disease control and prevention; No. 568, mingshi road, jianqiao town, jianggan district, Hangzhou; Jinhui1206@163.com; To the editor, More than 1.2 million cases of Coronavirus disease 2019 (COVID-19) [1] had been confirmed worldwide as of April 6, 2020. With the increase in overseas returnees and visitors to China, Hangzhou sets up a working group, including health-care workers and staffs from public security, transportation, foreign affairs and other non-medical system, responsible for quarantine work. Proper wear and removal of personal protective equipment (PPE) becomes a critical measure to ensure the safety of frontline personnel. This study intends to carry out the practice of pollutant simulation, so as to grasp the key point of PPE removal and guarantees the safety of frontline staffs. We trained 140 staffs from the working group for the theory of PPE in batches, followed by one-to-one field practice of wear and removal of PPE. Forty-six frontline staffs from non-medical systems were selected to carry out the pollutant simulation test. According to the characteristics of the phosphors which can be identified by naked eyes under ultraviolet irradiation, we dissolved the phosphors in gel -like ethanol as pollutant. We applied the phosphors on the outer surface of the gloves, forearms of the whole-body protective gowns, the outer surface of the gown hat, and the bare outer surface of kn95 respirator. After removing the PPE, we irradiated the clothes and the exposed skin through the ultraviolet lamp to show the risk points during the removal process. In the pollutant simulation test, 21 staffs polluted the upper chest, accounting for 45.7% of the total, followed by 20 and 15 staffs polluted the hands and the front chest, accounting for 43.5% and 32.6% of the total, respectively. The number of people with face and back pollution was less than 10%.See table 1 for details. The correct use of PPE is an effective way to ensure the safety of personnel, and improper wear and removal will bring potential harm to users [2] . In this study, 45.7% of the workers caused the upper chest pollution during the unloading process of PPE, and the main pollution source was from the lower edge of outer surface of kn95 respirator. When the user lowered his head in the removal process, the chest was contaminated. Therefore, in the wear process, it's necessary to protect the external surface of the kn95 respirator with the placket seal. 43.5% users contaminated their hands during the removal of PPE. In this study, 50% of the workers' hand hygiene actions were not standardized, and 39.1% of the workers forgot to do hand hygiene at least once when they took off PPE. Hand hygiene is considered the most economical, convenient and efficient way to control hospital infection [3, 4] ,it is of great significance to enhance the awareness of hand hygiene [5] . To reduce the risk of infection, we should pay more attention to the removal of PPE without contamination as well as its supply. The outbreak of COVID-19: An overview Air, Surface Environmental, and Personal Protective Equipment Contamination by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) From a Symptomatic Patient The 'My five moments for hand hygiene' concept for the overcrowded setting in resource-limited healthcare systems Potential utilities of mask wearing and instant hand hygiene for fighting SARS-CoV-2 Practical recommendations for critical care and anesthesiology teams caring for novel coronavirus (2019-nCoV) patients