key: cord-0865108-hyy08odl authors: Stilma, Willemke; van Meenen, David M. P.; Valk, Christel M. A.; de Bruin, Hendrik; Paulus, Frederique; Serpa Neto, Ary; Schultz, Marcus J. title: Incidence and Practice of Early Prone Positioning in Invasively Ventilated COVID-19 Patients—Insights from the PRoVENT-COVID Observational Study date: 2021-10-19 journal: J Clin Med DOI: 10.3390/jcm10204783 sha: ad6f18ea074adc4a0f5d1b24072bd0bf5b1d4792 doc_id: 865108 cord_uid: hyy08odl We describe the incidence and practice of prone positioning and determined the association of use of prone positioning with outcomes in invasively ventilated patients with acute respiratory distress syndrome (ARDS) due to coronavirus disease 2019 (COVID-19) in a national, multicenter observational study, performed at 22 intensive care units in the Netherlands. Patients were categorized into 4 groups, based on indication for and actual use of prone positioning. The primary outcome was 28-day mortality. Secondary endpoints were 90-day mortality, and ICU and hospital length of stay. In 734 patients, prone positioning was indicated in 60%—the incidence of prone positioning was higher in patients with an indication than in patients without an indication for prone positioning (77 vs. 48%, p = 0.001). Patients were left in the prone position for median 15.0 (10.5–21.0) hours per full calendar day—the duration was longer in patients with an indication than in patients without an indication for prone positioning (16.0 (11.0–23.0) vs. 14.0 (10.0–19.0) hours, p < 0.001). Ventilator settings and ventilation parameters were not different between the four groups, except for FiO(2) which was higher in patients having an indication for and actually receiving prone positioning. Our data showed no difference in mortality at day 28 between the 4 groups (HR no indication, no prone vs. no indication, prone vs. indication, no prone vs. indication, prone: 1.05 (0.76–1.45) vs. 0.88 (0.62–1.26) vs. 1.15 (0.80–1.54) vs. 0.96 (0.73–1.26) (p = 0.08)). Factors associated with the use of prone positioning were ARDS severity and FiO(2). The findings of this study are that prone positioning is often used in COVID-19 patients, even in patients that have no indication for this intervention. Sessions of prone positioning lasted long. Use of prone positioning may affect outcomes. Patients with acute respiratory distress syndrome (ARDS) have been shown to benefit from early prone positioning if hypoxemia is severe and refractory through an increase in the fraction of inspired oxygen (FiO 2 ) > 60% and higher positive end-expiratory pressure (PEEP) [1, 2] . Especially patients with focal consolidations could profit from this intervention [3] , as higher PEEP may be ineffective and could even cause overdistension. Before the coronavirus disease 2019 (COVID-19) pandemic, prone positioning remained remarkably underused [4, 5] . Invasively ventilated patients with ARDS due to COVID-19 often have an indication for prone positioning. Indeed, these patients often have severe hypoxemia. Additionally, consolidation may behave as focal lesions [6, 7] , which is another reason to apply prone positioning early after the start of invasive ventilation [8] . Last but not least, hypoxemia could also be a consequence of pulmonary embolism, for which higher PEEP is not helpful. Several recent reports in COVID-19 patients have shown frequent use of prone positioning, but with a remarkable variance in incidence and practice [6, [8] [9] [10] [11] . The purpose of this current analysis of a national multicenter study of COVID-19 patients admitted to the intensive care unit (ICU) for invasive ventilation early in the pandemic, named 'PRactice of VENTilation in COVID-19 (PRoVENT-COVID) [12] , was to study the incidence and practice of prone positioning in this cohort. We tested the hypothesis that prone positioning improves the outcome of COVID-19 patients. We also wished to determine what factors were associated with its use. The PRoVENT-COVID study is an observational cohort study undertaken at 22 ICUs from 1 March 2020 until 1 June 2020 in the Netherlands-in this study, we enrolled~40% of all patients that needed invasive ventilation during the first wave of the national outbreak [13] . The study protocol [12] and the statistical analysis plan for the current analysis were prepublished [14] . The study protocol was approved by the ethics committee in Amsterdam UMC, Amsterdam, the Netherlands (registration number W20_157 # 20.171); the need for individual patient informed consent was waived due to the observational nature of the study. The study was registered at clinicaltrials.gov on 15 April 2020 with study identifier NCT04346342. Consecutive patients were enrolled in the PRoVENT-COVID study if (1) age was >18 years; (2) they were admitted to one of the participating ICUs; and (3) they had received invasive ventilation for respiratory failure related to COVID-19 that was confirmed by a reverse transcriptase-polymerase chain reaction for SARS-CoV-2. The PRoVENT-COVID study had no exclusion criteria. For the current analysis, we excluded patients who were transferred from or to another ICU during the first days of invasive ventilation, as it could be that prone positioning was delayed because of an imminent transport and also because data on the use of prone positioning could not be assessed in non-participating centers. We collected demographic data, including disease severities and the medical history at baseline. ARDS severity was scored as mild, moderate or severe, in accordance with the current definition for ARDS [15] . Ventilator settings and ventilation parameters were collected every 8 h, and use and timing of prone positioning and use of neuromuscular blocking agents (NMBA) were collected in the first 4 calendar days of invasive ventilation. Chest X-rays and lung CT-scans were made at initiation of invasive ventilation. The X-rays were coded in quadrants and the CT-scans as a %. This was based on the interpretation of trained data collectors: all data collectors had a medical background and had received additional training regarding the chest X-rays and lung CT-scan assessment before the start of data collection. Follow-up was complete up to day 90, and included timing of liberation from invasive ventilation, ICU and hospital discharge, and life status at ICU and hospital discharge, and at day 28 and day 90. Patients were categorized into 4 groups based on indication for (yes or no) and the use of (yes or no) early prone positioning. A patient was labeled to have an indication for prone positioning if PaO 2 /FiO 2 ratio < 150 mmHg, at PEEP of ≥5 cm H 2 O and FiO 2 ≥ 0.6 [1] for at least 2 consecutive time points within the first 32 h after the start of invasive ventilation. The primary endpoint of this analysis was 28-day mortality. Secondary outcomes were 90-day mortality, and ICU-and hospital length of stay (LOS). We did not perform a formal sample size calculation; instead, the number of available patients served as the sample size. The day of intubation, which in theory could last from 1 min to 23 h and 59 min, was named 'day 0'. Successive days were named 'day 1', 'day 2' and 'day 3'. Categorical patient variables are presented as numbers and percentages, and continuous data as medians with interquartile ranges. With regard to the primary endpoint, there were no missing data. The amount of missing data of other variables was low, <5%. Incidence of prone positioning is expressed as numbers and percentages variables. Timing and duration of prone positioning are expressed in the number of hours from the start of invasive ventilation, and the total number of hours per full calendar day. To assess differences among the 4 groups a chi-squared test and Kruskal-Wallis test were used where appropriate. Ventilatory variables and parameters over the first 4 calendar days were compared using a Kruskal-Wallis test and were presented in cumulative distribution plots and line graphs displaying the 4 groups of interest. For each day, ventilatory variables at the moment of the worst PaO 2 /FiO 2 for that day were used, assuming these were collected at the moment the patient was in a supine position. Hazard ratios (HRs) for 28-day and 90-day mortality were compared between the 4 groups using a (shared-frailty) Cox proportional hazard model, with the center as frailty. HRs for ICU length of stay, hospital length of stay and ventilator free days, were compared using a competing risk analysis with the center as a random effect. Kaplan-Meier curves were constructed for all outcomes of interest. Predefined variables assessed for the final models were severity of ARDS [10] , PEEP, FiO 2 , body mass index (BMI), use of NMBAs and tidal volume per predicted body weight. If these variables had a p < 0.20 in the univariable model, they were included in the multivariable model. Covariates used for the final model were the variables with a p < 0.05 in the multivariable model; the covariates used in the univariable, and multivariable models are reported in Table S1 . This analysis was repeated to compare patients having an indication for and receiving prone positioning and patients having an indication for but not receiving prone positioning. An adjusted mixed-effect model with the center as a random effect was used to determine which factors had an association with use of prone positioning. Variables included in this model were severity of ARDS [16] , PEEP, FiO 2 , body mass index (BMI) and hypercapnia. As a posthoc analysis, a time-dependent Cox regression analysis was performed. All models were checked for collinearity. All analyses were conducted in R v.4.0.3 (R Foundation for Statistical Computing: Vienna, Austria) [17] and a p < 0.05 was considered significant. Between 1 March and 1 June 2020, 22 ICUs were invited and accepted participation in the PRoVENT-COVID study. Of 1122 enrolled patients, 734 patients were eligible for the current analysis. The main reason for exclusion was an early transfer from or to a non-participating hospital (Figure 1 ). At the start of ventilation, patients that were placed in prone positioning had higher severities of ARDS, and PaO 2 /FiO 2 < 150 was more frequent in patients that had an indication for prone positioning (Table 1) . Additionally, in the group without an indication for prone positioning, the severity of ARDS and the number of patients with a PaO 2 /FiO 2 ≤ 150 mm Hg was higher in the group that received prone positioning than the group that was not placed in the prone position (Table 1) . NMBAs were used more often in patients having an indication than in patients not having an indication for prone positioning (60% vs. 52%). An adjusted mixed-effect model with the center as a random effect was used to determine which factors had an association with use of prone positioning. Variables included in this model were severity of ARDS [16] , PEEP, FiO2, body mass index (BMI) and hypercapnia. As a posthoc analysis, a time-dependent Cox regression analysis was performed. All models were checked for collinearity. All analyses were conducted in R v.4.0.3 (R Foundation for Statistical Computing: Vienna, Austria) [17] and a p < 0.05 was considered significant. Between 1 March and 1 June 2020, 22 ICUs were invited and accepted participation in the PRoVENT-COVID study. Of 1122 enrolled patients, 734 patients were eligible for the current analysis. The main reason for exclusion was an early transfer from or to a nonparticipating hospital (Figure 1 ). At the start of ventilation, patients that were placed in prone positioning had higher severities of ARDS, and PaO2/FiO2 < 150 was more frequent in patients that had an indication for prone positioning (Table 1) . Additionally, in the group without an indication for prone positioning, the severity of ARDS and the number of patients with a PaO2/FiO2 ≤ 150 mm Hg was higher in the group that received prone positioning than the group that was not placed in the prone position (Table 1) . NMBAs were used more often in patients having an indication than in patients not having an indication for prone positioning (60% vs. 52%). Prone positioning was used in 438/734 (60%) patients. Incidence of prone positioning was higher in patients having an indication than in patients not having an indication for prone positioning (77% vs. 48%; p < 0.001). For patients who were placed in the prone position, the median first day of proning was day 0 (0-1) and received prone positioning over a median of 3 (2-3) days; this was similar in patients with and without an indication. At the start of ventilation, peak airway pressure, driving pressure, compliance, respiratory rate, FiO2, PaO2, and SaO2/FiO2 and PaO2/FiO2, and mechanical power were different between the 4 groups ( Table 3 ). In the group without an indication for prone posi- At the start of ventilation, peak airway pressure, driving pressure, compliance, respiratory rate, FiO 2 , PaO 2, and SaO 2 /FiO 2 and PaO 2 /FiO 2 , and mechanical power were different between the 4 groups (Table 3 ). In the group without an indication for prone positioning, invasive ventilation differed with regard to the peak and driving pressure. Both were higher in the patients that received prone positioning (Table 3) . Driving pressure, compliance, PaO 2 /FiO 2 , FiO 2 and PaCO 2 remained different between groups on successive days. Tidal volume was not different between groups on any day of collection of these data. PEEP was only different on day 2 and day 3; PaO 2 was different on day 0 and day 1 (Figure 3 , Figure 4 and Figures S1-S8) . Mortality at day 28 was lowest in patients with no indication for prone positioning-28.6% vs. 31.3% in patients that were not placed in prone position vs. patients that were placed in the prone position. Mortality at day 28 was highest in patients with an indication for prone positioning-41.3% vs. 34.1% in patients that were not placed in prone position vs. patients that were placed in the prone position. Differences between the four groups, though, did not reach statistical significance (p = 0.244). Differences in mortality at day 90 between groups followed a similar pattern (p = 0.100). ICU length of stay in patients that survived till ICU discharge was lower in patients that had no indication for prone positioning-a median of 16 (10-25) days vs. 19 days, in patients that were not placed in a prone position vs. patients that were placed in a prone position. ICU length of stay in patients that survived till ICU discharge was higher in patients that had an indication for prone positioning-a median of 22 (12-30) days vs. 21 (14-34) days in patients that were not placed in a prone position vs. patients that were placed in a prone position. Mortality at day 28 was lowest in patients with no indication for prone positioning-28.6% vs. 31.3% in patients that were not placed in prone position vs. patients that were placed in the prone position. Mortality at day 28 was highest in patients with an indication for prone positioning-41.3% vs. 34.1% in patients that were not placed in prone position vs. patients that were placed in the prone position. Differences between the four groups, though, did not reach statistical significance (p = 0.244). Differences in mortality at day 90 between groups followed a similar pattern (p = 0.100). ICU length of stay in patients that survived till ICU discharge was lower in patients that had no indication for prone positioning-a median of 16 (10-25) days vs. 19 days, in patients that were not placed in a prone position vs. patients that were placed in a prone position. ICU length of stay in patients that survived till ICU discharge was higher in patients that had an indication for prone positioning-a median of 22 (12) (13) (14) (15) (16) (17) (18) (19) (20) (21) (22) (23) (24) (25) (26) (27) (28) (29) (30) days vs. 21 days in patients that were not placed in a prone position vs. patients that were placed in a prone position. Hospital length of stay in patients that survived till hospital discharge was lower in patients with no indication for prone positioning-a median of 28 (20-40) days vs. 31 (22-) days in patients that were not placed in the prone position vs. patients that were placed in the prone position. Hospital length of stay in patients that survived till hospital discharge was higher in patients with an indication for prone positioning-a median of 31 Adjusted HRs were different between groups for mortality at day 90, ICU length of stay and hospital length of stay, but not for mortality at day 28 ( Figure 5 and Figure S9 ). not placed in the prone position vs. patients that were placed in the prone position. tilator free days at day 28 were low in patients with an indication for prone position a median of 0.0 (0.0-10.0) days vs. 0.0 (0.0-14.0) days, in patients that were not plac a prone position vs. patients that were placed in a prone position. Adjusted HRs were different between groups for mortality at day 90, ICU leng stay and hospital length of stay, but not for mortality at day 28 ( Figures 5 and S9 ). ARDS severity and FiO2 were the only factors that were independently assoc with the actual use of prone positioning (Table S2) . The time-dependent Cox regression analysis did not change the findings (Table Here, we describe the practice and outcome of prone positioning in patients ARDS due to COVID-19 that received invasive ventilation in the first 3 months of th tional outbreak in the Netherlands. The incidence of prone positioning was high, a patients not having an indication for this intervention. Sessions of prone positioning long and lasted longer in patients with an indication. ARDS severity and FiO2 pred the use of prone positioning. ARDS severity and FiO 2 were the only factors that were independently associated with the actual use of prone positioning (Table S2) . The time-dependent Cox regression analysis did not change the findings (Table S3) . Here, we describe the practice and outcome of prone positioning in patients with ARDS due to COVID-19 that received invasive ventilation in the first 3 months of the national outbreak in the Netherlands. The incidence of prone positioning was high, also in patients not having an indication for this intervention. Sessions of prone positioning were long and lasted longer in patients with an indication. ARDS severity and FiO 2 predicted the use of prone positioning. Our study confirms the high incidence of prone positioning in invasively ventilated COVID-19 patients, as found in other observational studies [6, 9, 11, 18] . Studies from before the COVID-19 pandemic showed a remarkable underuse of this intervention in patients with ARDS-in the LUNG SAFE study in 2014, overall use was 7.9%, and 16.3% in patients with severe ARDS [19] ; in the APRONET study in 2016, overall use was 13.7%, and 32.9% of patients with severe ARDS [5] . There are several reasons why prone positioning is used more often in COVID-19 patients. It could simply be that the increase of use has continued after LUNG SAFE and APRONET-the implementation of interventions with proven benefits can take many years, also in the ICU setting [20] . It could also be that the poor results of randomized clinical trials that tested alternative ways to improve outcomes, like higher PEEP and recruitment maneuvers, have had a positive effect on the use of prone positioning. Last but not least, it could be that COVID-19 ARDS presents as a form of lung injury that may respond better to prone positioning than other forms of ARDS [3] . Indeed, the findings of one randomized clinical trial suggest that prone positioning may be better than higher PEEP in ARDS patients with non-recruitable lung lesions, which may be typical in COVID-19 ARDS, at least at the initiation of invasive ventilation [3, 21] . The high incidence of prone positioning was notable in patients not having an indication for this intervention. This may also have been the case in other cohorts, as the reported overall PaO 2 /FiO 2 ratio in other studies was comparable to that in our cohort [5, 8, 14] , suggesting a similar distribution of ARDS severities and with that a comparable rate of indication for prone positioning. In addition, some of the patients without an indication for prone positioning were actually placed in the prone position. This group had median lower PaO 2 /FiO 2 ratios, which could be seen as an indication to initiate prone positioning by the clinician. Whether the results of the chest X-rays and lung CT-scans were an indication for the clinician to initiate prone positioning could not be collected. Therefore, we could not comment on or analyze the relationship between imaging and the indication for prone positioning in this cohort. In our data, there was no difference in mortality at day 28 between groups, but there was a difference in mortality at day 90. Duration of invasive ventilation is remarkably high in COVID-19 patients, and so is LOS in ICU in these patients [11] [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] [26] [27] [28] [29] [30] . This can explain why 28-mortality was not different between the groups, while 90-day mortality was. On the one hand, it could suggest the benefit of this intervention in ARDS due to COVID-19, in line with the findings of the seminal study in patients with ARDS not related to COVID-19 in France [1] . This finding is also in line with the results of one meta-analysis of studies in invasively ventilated COVID-19 patients [23] . On the other hand, it could be that this intervention was foregone in patients with a poor outcome, or in patients with treatment directives. Information on this was not collected in the PRoVENT-COVID study. This explanation, however, seems less likely as there were no differences in any baseline characteristic. The use of NMBA during prone positioning was remarkably lower than in previous studies in the pre-COVID era, in which the incidences were as high as 72% [23] to 88% [11] . It should be noted, though, that recommendations regarding the use of NMBA in ARDS patients [24] may have become obsolete after the publication of the more recent ROSE trial [25] . Additionally, recommendations for the use of NMBA, as well as the actual use of NMBA during prone positioning, may depend on local practices, and maybe even on the experiences of the healthcare workers that had to take care of patients in the overwhelming first wave of the COVID-19 pandemic. Notably, a recent study in COVID-19 patients showed NMBA use to be associated with a higher risk of and a longer duration of ventilation and longer ICU LOS, even after propensity matching [26] . A recent study in patients with ARDS due to COVID-19 showed that sustained improvements in oxygenation in response to the first prone positioning session are associated with better outcomes [27] . Recently, the recruitment-to-inflation ratio was suggested as a bedside tool to identify patients that have a high chance of responding well to lung recruitment maneuvers [28] . Unfortunately, we were unable to separate patients based on these approaches, due to the way data were collected. Prone positioning could come with procedure-related adverse effects [5, 29] . We did not collect these data. It could be hypothesized that the incidence of adverse events during a pandemic is high due to the stressful and demanding situation, with increased workloads and the lack of experienced staff. However, when dedicated prone position teams are present, as was often the case in the centers in the Netherlands early in the pandemic, the rate of procedure-related adverse events could also be low [30] . The only two factors that had an association with the actual use of prone positioning were ARDS severity and FiO 2 . This is in line with an earlier observation from before the COVID-19 pandemic [5] . In that study, the major reason for not placing a patient in the prone position was that clinicians deemed hypoxemia not being severe enough. In the current analysis, a PaO 2 /FiO 2 ratio < 150 mmHg at two successive observations was used as a cutoff for the indication for prone positioning. This is more strict than in the previous study. This study has strengths. First, the data were collected in a short time frame during which general care for COVID-19 patients did not change. Second, the study was designed to minimize bias by strictly adhering to a predefined statistical analysis plan. Third, the study involved one-third of all COVID-19 ARDS patients receiving invasive ventilation in the first months of the national outbreak in the Netherlands, and patients were enrolled in 22 ICUs from university-affiliated hospitals, teaching hospitals and non-teaching hospitals, contributing to its generalizability. PRoVENT-COVID also has limitations. As in any observational study, the knowledge that care data were being captured could have interfered with practice-for instance, doctors and nurses in participating centers could have been keener to use prone positioning. In line with the study design, the use of late prone positioning, i.e., after the first 4 days of invasive ventilation, was not collected. This means that we could not report on the associations of late prone positioning, if that happened, with outcomes. Additionally, it should be realized that reasons to exclude patients from prone positioning, such as recent tracheal surgery or sternotomy, pregnancy or presence of wounds or burns, were not collected. It is conceivable, though, that these contra-indications were barely present in this cohort. Nevertheless, this may have introduced misclassification bias. The selection of ICUs was based on the personal contacts between steering committee members and ICUs that participated in recent research projects of ventilation, which could have resulted in an overrepresentation of units with more experience in prone positioning, and therefore a higher incidence. Similar to other epidemiological studies, access to patients' data was restricted to data collectors who were granted access only to patients that were labeled eligible for participation by the local doctors-thus, we could not control whether all COVID-19 patients receiving invasive ventilation in participating ICUs were enrolled. Lastly, the national character of PRoVENT-COVID may make these results not representative for other countries. The findings of this study extend our understanding of the incidence and practice of prone positioning in patients with ARDS due to COVID-19, and the association of this intervention with outcomes. Our findings may have important suggestions for clinical management. In this national cohort of patients with ARDS due to COVID-19, prone positioning was frequently used, even in patients that did not have an indication for this intervention. Prone positioning may improve the outcome of invasively ventilated patients with an indication for this intervention. Factors that had an association with its use were ARDS severity and set FiO 2 . Table S3 : Time dependent cox regression analysis, Figure S1 : Distribution curves 4 groups tidal volume, PEEP, driving pressure and compliance day 1, Figure S2 : Distribution curves 4 groups P/F ratio, FiO2, PO2, PCO2 day 1, Figure S3 : Distribution curves 4 groups tidal volume, PEEP, driving pressure and compliance day 2, Figure S4 : Distribution curves 4 groups P/F ratio, FiO2, PO2, PCO2 day 2, Figure S5 : Distribution curves 4 groups tidal volume, PEEP, driving pressure and compliance day 3, Figure S6 : Distribution curves 4 groups P/F ratio, FiO2, PO2, PCO2 day 3, Figure S7 : Line graphs tidal volume, driving pressure, PEEP and compliance day 0, 1, 2, 3, Figure S8 : Line graphs P/F ratio, PO2, PCO2, FiO2 for day 0, 1, 2, 3, Figure S9 : Outcomes. Prone Positioning in Severe Acute Respiratory Distress Syndrome Effect of prone positioning during mechanical ventilation on mortality among patients with acute respiratory distress syndrome: A systematic review and meta-analysis Personalised mechanical ventilation tailored to lung morphology versus low positive end-expiratory pressure for patients with acute respiratory distress syndrome in France (the LIVE study): A multicentre, single-blind, randomised controlled trial LUNG SAFE Investigators; et al. Patterns of Use of Adjunctive Therapies in Patients with Early Moderate to Severe ARDS: Insights from the LUNG SAFE Study A prospective international observational prevalence study on prone positioning of ARDS patients: The APRONET (ARDS Prone Position Network) study Correction to: Clinical features, ventilatory management, and outcome of ARDS caused by COVID-19 are similar to other causes of ARDS High versus low PEEP in non-recruitable collapsed lung tissue: Possible implications for patients with COVID-19 Mechanical ventilation parameters in critically ill COVID-19 patients: A scoping review Epidemiology, clinical course, and outcomes of critically ill adults with COVID-19 Pathophysiology of COVID-19-associated acute respiratory distress syndrome: A multicentre prospective observational study Clinical characteristics and day-90 outcomes of 4244 critically ill adults with COVID-19: A prospective cohort study PRactice of VENTilation in Patients with Novel Coronavirus Disease (PRoVENT-COVID): Rationale and protocol for a national multicenter observational study in The Netherlands Ventilation management and clinical outcomes in invasively ventilated patients with COVID-19 (PRoVENT-COVID): A national, multicentre, observational cohort study PRoVENT-COVID)-Rationale, Study Protocol and Statistical Analysis Plans for a National Multicenter Observational Study in The Netherlands The Berlin definition of ARDS: An expanded rationale, justification, and supplementary material Acute respiratory distress syndrome: The Berlin Definition R Foundation for Statistical Computing. R: A Language and Environment for Statistical Computing; R Foundation for Statistical Computing Prone Positioning and Survival in Mechanically Ventilated Patients with Coronavirus Disease 2019-Related Respiratory Failure Patterns of Care, and Mortality for Patients with Acute Respiratory Distress Syndrome in Intensive Care Units in 50 Countries The answer is 17 years, what is the question: Understanding time lags in translational research Battling COVID-19-related mortality: From a fight for ventilators to a cry for oxygen Patient characteristics, clinical course and factors associated to ICU mortality in critically ill patients infected with SARS-CoV-2 in Spain: A prospective, cohort, multicentre study Effect of prone versus supine position in COVID-19 patients: A systematic review and meta-analysis Clinical practice guidelines for sustained neuromuscular blockade in the adult critically ill patient: 2016 updateexecutive summary National Heart, Lung, and Blood Institute PETAL Clinical Trials Network Early Neuromuscular Blockade in the Acute Respiratory Distress Syndrome COVID-19 Critical Care Consortium Neuromuscular Blocking Agents in Critically-Ill COVID-19 Patients Requiring Mechanical Ventilation Sustained oxygenation improvement after first prone positioning is associated with liberation from mechanical ventilation and mortality in critically ill COVID-19 patients: A cohort study Differential effects of prone position in COVID-19-related ARDS in low and high recruiters A Comprehensive Review of Prone Position in ARDS Nursing care and prevalence of adverse events in prone position: Characteristics of mechanically ventilated patients with severe SARS-CoV-2 pulmonary infection The authors declare no conflict of interest. The study protocol was approved by the ethics committee in Amsterdam UMC, Amsterdam, the Netherlands (registration number W20_157 # 20.171); the need for in-dividual patient informed consent was waived due to the observational nature of the study.Informed Consent Statement: Patient consent was waived due to the observational nature of the study.Data Availability Statement: Access to source data will be made available through local, regional and national anonymized datasets on request, and after agreement of the PRoVENT-COVID steering committee.