key: cord-0864604-raqropjb
authors: Peng, Tsui‐Yi; Lee, Kuan‐Rong; Tarn, Woan‐Yuh
title: Phosphorylation of the arginine/serine dipeptide‐rich motif of the severe acute respiratory syndrome coronavirus nucleocapsid protein modulates its multimerization, translation inhibitory activity and cellular localization
date: 2008-07-09
journal: FEBS J
DOI: 10.1111/j.1742-4658.2008.06564.x
sha: 4f394214d79be643597d3716bbf80a061a6ad04d
doc_id: 864604
cord_uid: raqropjb

Coronavirus nucleocapsid protein is abundant in infected cells and participates in viral RNA replication and transcription. The central domain of the nucleocapsid protein contains several arginine/serine (RS) dipeptides, the biological significance of which has not been well investigated. In the present study, we demonstrate that the severe acute respiratory syndrome coronavirus nucleocapsid protein is phosphorylated primarily within the RS‐rich region in cells and by SR protein kinase 1 in vitro. The nucleocapsid protein could suppress translation and its RS motif is essential for such an activity. Moreover, phosphorylation of the RS motif could modulate the translation inhibitory activity of the nucleocapsid protein. We further found that RS motif phosphorylation did not significantly affect RNA binding of the nucleocapsid protein but impaired its multimerization ability. We observed that the nucleocapsid protein could translocate to cytoplasmic stress granules in response to cellular stress. Deletion or mutations of the RS motif enhanced stress granule localization of the nucleocapsid protein, whereas overexpression of SR protein kinase 1 inhibited nucleocapsid protein localization to stress granules. The nucleocapsid protein lacking the RS motif formed high‐order RNP complexes, which may also account for its enhanced stress granule localization. Taken together, phosphorylation of the severe acute respiratory syndrome‐CoV nucleocapsid protein modulates its activity in translation control and also interferes with its oligomerization and aggregation in stress granules.

Coronavirus nucleocapsid protein is abundant in infected cells and participates in viral RNA replication and transcription. The central domain of the nucleocapsid protein contains several arginine ⁄ serine (RS) dipeptides, the biological significance of which has not been well investigated. In the present study, we demonstrate that the severe acute respiratory syndrome coronavirus nucleocapsid protein is phosphorylated primarily within the RS-rich region in cells and by SR protein kinase 1 in vitro. The nucleocapsid protein could suppress translation and its RS motif is essential for such an activity. Moreover, phosphorylation of the RS motif could modulate the translation inhibitory activity of the nucleocapsid protein.

We further found that RS motif phosphorylation did not significantly affect RNA binding of the nucleocapsid protein but impaired its multimerization ability. We observed that the nucleocapsid protein could translocate to cytoplasmic stress granules in response to cellular stress. Deletion or mutations of the RS motif enhanced stress granule localization of the nucleocapsid protein, whereas overexpression of SR protein kinase 1 inhibited nucleocapsid protein localization to stress granules. The nucleocapsid protein lacking the RS motif formed high-order RNP complexes, which may also account for its enhanced stress granule localization. Taken together, phosphorylation of the severe acute respiratory syndrome-CoV nucleocapsid protein modulates its activity in translation control and also interferes with its oligomerization and aggregation in stress granules. and packaging of viral genomic RNA [8] [9] [10] . Moreover, it binds to the 5¢ and ⁄ or 3¢ end of the genomic RNA [11] [12] [13] and may participate in viral genome replication and subgenomic mRNA transcription [14, 15] . However, other evidence suggests that the N protein is dispensable for these processes [16] . In addition, the mouse hepatitis virus (MHV) N protein stimulates translation of a reporter mRNA containing an intact MHV 5¢-untranslated region, probably by binding to a tandem repeat of UCYAA in the leader sequence [17] . By contrast, recent evidence indicates that the SARS-CoV N protein interferes with translation through its interaction with cellular translation elongation factor 1a [18] . In addition, the SARS-CoV N protein can inhibit the activity of cellular cyclin-dependent kinases and thereby perturb S phase progression of virusinfected cells [19, 20] . Therefore, coronavirus N proteins may affect various cellular functions.

Within various coronaviruses, the N protein varies from 377 to 455 amino acid residues in length. Although the sequence conservation between N proteins is relatively low [3, 21] , they are likely to adopt a common secondary structure essentially consisting of two functional domains. The N-terminal domain interacts with RNA through a structural module rich in positively charged residues [22] [23] [24] . The RNA binding capacity of the N protein is critical for viral infectivity [24] . The C-terminal domain folds into a b-sheet platform engaged in homodimerization [24, 25] and may also confer the RNA binding activity [10] . Moreover, RNA binding may promote multimerization of the N protein, implicating a nucleocapsid formation mechanism [10, 23, 26] .

Between the two functional domains is a structurally flexible segment containing several arginine ⁄ serine (RS)-dipeptides. This RS-rich motif is characteristic of cellular precursor mRNA (pre-mRNA) splicing factors, termed SR proteins [27] . The RS domain is dynamically phosphorylated by several SR protein specific kinases, such as those of the SR protein kinase (SRPK) and Clk families [28] . Phosphorylation of the RS domain modulates the activity, protein-protein interactions and subcellular localization of SR proteins [29] . Coronavirus N proteins are phosphorylated in host cells and in virions [25, 30, 31] and it has been reported that phosphorylation affects the RNA binding specificity and nucleocytoplasmic shuttling of the N proteins [25, 32] . Indeed phosphorylation can occur within the RS motif of coronavirus N proteins [19, 33] and this motif may play a role in C-terminal domain dimerization [26] . Nevertheless, whether phosphorylation of the RS motif can modulate the functions of N proteins remains to be examined in detail.

Coronavirus N proteins localize to both the cytoplasm and the nucleolus in virus-infected cells [34] [35] [36] and can shuttle between the nucleus and the cytoplasm [37] . Nucleolar localization of N protein requires regions in the protein that are rich in arginine residues and is likely cell cycle-dependent [20, 35, 36] . The avian infectious bronchitis virus N protein indeed interacts with and colocalizes with the nucleolar proteins nucleolin and fibrillarin [38, 39] . However, the ability of nucleolar localization varies between N proteins of different coronaviruses [36] . The SARS-CoV N protein is poorly localized to the nucleolus [36] . In the present study, we found that the SARS-CoV N protein appeared in cytoplasmic stress granules (SGs). When eukaryotic cells encounter environmental stress, mRNA metabolism is reprogrammed to adapt to stress-induced damage. Translationally stalled mRNAs together with a number of translation initiation factors and RNA-binding proteins are deposited into SGs [40] . Formation of SGs can also be induced by overexpression of the prion-like RNA binding protein TIA-1 [41] . Upon stress induction, TIA-1 forms aggregates in SGs and may play a role in translation inhibition [41] .

In the present study, we examined phosphorylation of the SARS-CoV N protein. Our data provide evidence that phosphorylation of the N protein primarily occurs within its RS-rich motif and may affect its oligomerization, translation inhibitory activity and subcellular localization.

Phosphorylation of the RS-rich motif of the SARS-CoV N protein Coronavirus N proteins are phosphoproteins [30, 32] . The N protein of all coronaviruses, including the SARS-CoV, contains an RS-rich motif ( Fig. 1 ) that likely provides potential phosphorylation sites for multiple cellular kinases [32] . We predicted that N proteins, due to their similarity with cellular SR proteins in the RS-rich motif, may serve as a substrate of SR protein specific kinases. To study phosphorylation of SARS-CoV N protein RS domain, we overexpressed FLAG-tagged N protein and the RS-deleted mutant (NDRS) in HEK293 cells. Transfected cells were incubated with [ 32 P]orthophosphate for labeling. Anti-FLAG immunoprecipitation of the full-length N protein from the cell lysate revealed a 32 P-labeled band at approximately 52 kDa ( Fig. 2A, lane 1) , similar to previous reports [42] , indicating that the SARS-CoV N protein was phosphorylated in vivo. However, inefficient labeling of NDRS ( Fig. 2A, lane 2) suggested that the RS motif is the major phosphorylation site for the SARS-CoV N protein in cells. We next examined whether the SR protein kinase SRPK1 can phosphorylate the N protein within its RS-rich motif. Full-length N and NDRS were each fused to glutathione S-transferase (GST) and subjected to in vitro phosphorylation using purified SRPK1. Figure 2B shows that only full-length N protein was phosphorylated by SRPK1. However, neither another SR protein kinase, Clk1, nor protein kinase A was able to phosphorylate the N protein in vitro (see supplementary Fig. S1 ). To avoid the possibility that inefficient phosphorylation of NDRS resulted from its improper folding, recombinant GST-NDRS protein was analyzed by CD spectroscopy. The CD spectrum of purified NDRS suggested that this truncated protein still adopted an ordered conformation ( Fig. 2C ) and was also similar to that of the full-length N protein (data not shown). Taken together, these data suggest that the RS-rich region of the SARS-CoV N protein is possibly phosphorylated by SRPK1.

The RS motif of the SARS-CoV N protein is divergent from that of canonical SR proteins and contains fewer RS dipeptides. To determine which serines might be the major phosphorylation sites, we made a series of serine to alanine substitution mutants and investigated their phosphorylation using SRPK1. As shown in Fig. 2D , increasing the number of alanine substitution gradually decreased the phosphorylation level of the N protein. This result indicated that multiple serines are phosphorylated, and was consistent with the observations made for other SR proteins [43] . However, because N-8A was much poorly phosphorylated compared to N-6A (Fig. 2D , lanes 3 and 4), S 203 S 204 might serve as the primary site of SRPK1-mediated phosphorylation.

Previous reports indicate that MHV infection induces host translational shut-off [44] . Coronavirus N proteins are primarily distributed throughout the cytoplasm, with a higher concentration within nucleoli [21, 35, 39] and, thus, have the potential to interfere with ribosome biogenesis or translation in host cells.

To test whether the SARS-CoV N protein plays a role in translation control and whether phosphorylation modulates its activity, we performed an in vitro translation assay. Using a firefly luciferase reporter, we titrated recombinant GST-N or GST-NDRS protein into the reticulocyte lysate. Both the protein level and activity of the luciferase were measured, which may directly reflect the translation activity because luciferase mRNA levels were similar between treatments ( Fig. 3 , bottom). The GST-N protein suppressed luciferase translation in a dose-dependent manner but this translation suppressive effect was attenuated upon phosphorylation by SRPK1 (Fig. 3 ). GST-NDRS or GST control had no significant effects on translation of the luciferase mRNA. Therefore, the SARS-CoV N protein might possess translation suppression activity that requires its RS motif and, thus, could be modulated by phosphorylation.

To better understand the effect of RS motif phosphorylation on the biological function of the N protein, we Representative autoradiograms show the resulting firefly luciferase protein; Coomassie blue staining shows titrated N protein (N) and a reticulosyte lysate protein (*) as loading control. The graph shows relative translation efficiency obtained by comparison with the reaction without N protein; data are the mean ± SD values are from three independent experiments. In vitro translation reactions contained 1 lg of indicated recombinant protein as well as 32 P-labeled luciferase reporter mRNA as tracer. After incubation, the level and the integrity of radioisotope labeled RNA were examined on a denaturing 4% polyacrylamide gel.

T Figure 4A shows that His-N was 32 P-labeled after phosphorylation and protein phosphatase treatment removed 32 slight mobility shift of the N protein (lane 2), possibly indicating its stoichiometric phosphorylation. Next, electrophoretic mobility shift assay showed that nonphosphorylated His-N bound an approximately 110 nucleotide RNA probe with an apparent dissociation constant of 52.9 nm, comparable to that reported previously [45, 46] . Moreover, His-N appeared to form oligomers in a concentration-dependent manner (Fig. 4B,C) . The phosphorylated N protein also bound this RNA probe and its dissociation constant was determined to be 66.7 nm, which was similar to that of nonphosphorylated N protein (Fig. 4C) . However, formation of high-order N protein RNP complexes appeared to be impaired when the N protein was phosphorylated (Fig. 4D) . Chemical crosslinking of the N protein confirmed that phosphorylated N was less capable of forming oligomers than the nonphosphorylated one (Fig. 4E) . Therefore, it is likely that phosphorylation of the RS motif interferes with oligomerization of the N protein.

Because the RS domain can modulate subcellular localization of cellular SR proteins [45] , we next examined whether the RS motif of SARS-CoV N protein has this activity. When the FLAG-tagged NDRS fusion protein was transiently expressed in HeLa cells, approximately 5% of transfected cells showed a punctate staining pattern (Fig. 5A ). This granule-like localization pattern was also observed with the full-length N protein, albeit rarely (approximately 1% of the transfected cells). Although this granule staining pattern was observed only in a few percent of N or NDRSprotein expressing cells under normal cell conditions, it was greatly enhanced upon arsenite treatment (> 95% transfected cells; Fig. 5B ). Indeed both N and NDRS colocalized with endogenous poly(A)-binding protein 1 (PABP1) and transiently expressed TIA-1 (Fig. 5B) , both of which are SG components [40] .

To distinguish whether the RS motif deletion or a lack of phosphorylation enhanced N protein localization in SGs, we examined the cellular localization of two RS motif mutants, N-6A and N-14A. Both mutants showed a higher tendency towards SG localization than the wild-type N (Fig. 5A) , suggesting that SG localization of the N protein primarily resulted from its hypophosphorylation. Moreover, the N-terminal (N NT ) but not the C-terminal (N CT ) part of the N protein appeared to be responsible for granule localization (Fig. 5A) . We apparently reasoned that the N-terminal domain contains the RS motif and confers RNA binding ability [10, 22] and is therefore capable of forming granules. Next, we evaluated SRPK1-mediated RS motif phosphorylation in modulating SG localization or retention of the N protein. FLAG-tagged N protein and HA-tagged SRPK1 were transiently coexpressed in HeLa cells. In the presence of overexpressed SRPK1, the N protein was unable to localize to SGs, even after arsenite treatment of the cells (Fig. 6 , white arrows). However, SRPK1 overexpression could not disperse RS deletion or alanine substitution mutants to the cytoplasm (NDRS and N-14A; Fig. 6 ). Under this condition, PABP1, similar to NDRS and N-14A, showed a granular pattern (Fig. 6, lower panel) , indicating that SG assembly is not disturbed by overexpression of SRPK1. Therefore, phosphorylation of the RS motif might diminish N protein oligomerization (Fig. 4) and thereby prevent its aggregation in SGs. Together, the SARS-CoV N protein could target to SGs, reflecting its role in translation suppression. Moreover, phosphorylation of the RS motif modulates the ability of the N protein to form SGs.

The SARS-CoV N protein might regulate translation and could target to SGs; therefore, we evaluated whether it forms RNPs in host cells. Using glycerol gradient sedimentation, we observed that the N protein formed RNPs in cells because it was moved to lighter density fractions after RNase treatment (Fig. 7A ). Compared to full-length N, NDRS even migrated in heavier fractions of the sucrose density gradient (Fig. 7B) . The high-order NDRS complexes were also sensitive to RNase (data not shown). Therefore, removal of the RS motif from the N protein induced larger RNP formation, which may account for NDRS aggregation in SGs. The above data show that RS motif deletion induced high-order N protein-containing RNP formation. We inferred that this might result from hypophosphorylation of the NDRS protein.

The RS domain is a characteristic feature of cellular pre-mRNA splicing factors [27, 29] . Several viral proteins also contain various numbers of repeated RS dipeptides. The transactivator E2 protein of cutaneous papillomaviruses has a relatively long RS domain, which functions to recruit cellular splicing factors for cotranscriptional splicing regulation [47] . The core protein of hepatitis B virus (HBV) has an arginine-rich domain at the C-terminus that bears a few RS dipeptides. The HBV core protein can be phosphorylated by SRPK1 and SRPK2 [48] . Similar to the HBV core, coronavirus N proteins contain a short RS-rich motif (Fig. 1 ) and the SARS-CoV N protein might be phosphorylated by SRPK1 (Fig. 2) . We have examined whether the SARS-CoV N protein plays a role in pre-mRNA splicing due to the presence of the RS motif but, so far, we do not have any evidence to support this hypothesis (data not shown). In the present study, we provide evidence that the SARS-CoV N protein could suppress translation at least in vitro (Fig. 3) . The potential role of the N protein in translation control might correlate with its localization in the cytoplasmic SGs (Fig. 5 ) and is also in line with recent reports that coronavirus infection could cause translational shut-off in host cells and that the SARS-CoV N protein may execute this activity via its interact with elongation factor 1a [18, 44] . Although coronaviral N protein largely forms helical nucleocapsids with the viral RNA genome during infection [10] , how it participates in translation control in host cells and whether it has any substrate specificity or functions under certain cellular conditions remain to be studied in the future. Previous reports have suggested that the SARS-CoV N protein can act as a substrate of various kinases, such as cyclin-dependent kinases, glycogen synthase kinase, creatine kinase II and mitogen-activated protein kinase [32] . Our data show that multiple serine residues within the RS motif could be in vitro phosphorylated by SRPK1 (Fig. 2) . The SARS-CoV N protein is primarily distributed in the cytoplasm, coincident with the cellular localization of SRPK1. Coexpression of SRPK1 could modulate cellular localization of the N protein, suggesting that the N protein is a substrate of SRPK1 in cells (Fig. 6) . Phosphorylation of the transmissible gastroenteritis virus N protein also occurs on a moderately conserved serine within the RS motif, although which kinases could phosphorylate this serine is as yet unknown [33] . In the present study, we provide evidence that SRPK1-mediated RS motif phosphorylation influences the biochemical and biological activities of the SARS-CoV N protein. First, the potential translation suppression activity of the SARS-CoV N protein might be modulated by phosphorylation (Fig. 3) . Moreover, phosphorylation may also impact on its oligomerization, cellular localization and perhaps RNP complex formation (Figs 4-7) . The questions of whether SRPK1 phosphorylates the SARS-CoV N protein in cells particularly during viral infection and where this phosphorylation occurs remain to be answered.

A mammalian two-hybrid assay previously showed that the RS motif is directly involved in N protein selfinteraction [42] . However, other evidence indicated that the RS motif interferes with SARS-CoV N protein multimerization but this activity requires its C-terminal domain [26] . Our data show that RS motif phosphorylation partially impaired N protein multimerization (Fig. 4) . Perhaps such phosphorylation modulates the balance between N protein self-association and dissociation, which thereby impacts on its cellular functions. Multimerization of the N protein is necessary for nucleocapsid formation and assembly of the viral particles [42] . Thus, whether phosphorylation of the RS motif in virions could modulate N protein function in encapsulation of genomic RNA remains to be investigated. Moreover, we observed that deletion of the RS motif greatly enhanced association of the SARS-CoV N protein with cellular RNPs (Fig. 7) . Perhaps RS motif phosphorylation prevents nonspecific binding of the N protein to cellular RNP complexes and thus aids viral genome packaging into capsids; this possibility also remains to be tested.

During infection, coronaviral N protein participates in virus replication that probably occurs at the sites associated with ER-derived membrane tubules and vesicles [49] . Subsequently, viral nucleocapsids are transported to the budding sites in the Golgi region for viral particle formation. Although overexpressed, most coronavirus N proteins are located in the cytoplasm as well as in the nucleolus [34, 35] . Nevertheless, the SARS-CoV N protein does not localize substantially to the nucleolus [36, 50] , as also observed in the present study (Fig. 5 ). It has been proposed that the signals for nuclear and nucleolar targeting of the SARS-CoV N protein are poorly accessible to the nuclear import machinery due to phosphorylation regulation or conformational constrains [36, 50] . Nevertheless, the present study has revealed for the first time that overexpressed SARS-CoV N protein might localize to SGs in HeLa cells (Fig. 5) . Such an SG localization pattern was enhanced by deletion or phosphorylation site mutations of the RS motif and was obvious in stress-treated cells (Fig. 5) . SGs contain mRNPs whose translation is temporarily blocked [40] . Therefore, the N protein may sequester cellular mRNPs in SGs and inhibit their translation, possibly during viral infection. Nevertheless, the evidence demonstrating that RS motif phosphorylation reduced oligomerization of the N protein and prevented its aggregation in SGs is likely to be in accordance with the attenuation of its translation suppression activity.

The cDNA encoding the SARS-CoV N protein was kindly provided by K. Peck (Academia Sinica, Taipei, Taiwan). We generated the NDRS cDNA by ligating the PCR fragments encoding amino acid residues 1-175 and 215-422, respectively. The full-length N and NDRS cDNAs were each cloned into pcDNA3 (Invitrogen, Carlsbad, CA, USA) in-frame with the FLAG-epitope tag, and also into pCEP4 (Invitrogen) to generate the HA-tagged proteins. All the N protein mutants were generated from the FLAG-N construct using the QuikChange site-directed mutagenesis system (Stratagene, La Jolla, CA, USA); the sequences of these mutants were verified. The cDNAs encoding the N-terminal (residues 1-214) and C-terminal (residues 215-422) domain of the N protein and N-6A and N-14A were individually cloned into pCDNA3 (Invitrogen) in-frame fusion with the pre-engineered FLAG-tag. The pET11D-His-N vector was obtained from T. H. Huang (Institute of Biomedical Sciences, Academia Sinica) and used for production of the Histagged full-length N protein in Escherichia coli. The NDRS cDNA was appropriately cloned into pET15b (Novagen, Madison, WI, USA) for production of recombinant NDRS. The wild-type and mutant N and NDRS cDNAs were subcloned into pGEX-5X (GE Healthcare, Piscataway, NJ, USA) using EcoRI and SalI sites to generate the GSTfusion proteins. Subsequently, the cDNAs encoding mutant N proteins were cloned into pGEX-5X. The pET15b-FLAG used for in vitro transcription of an RNA probe was constructed by insertion of the FLAG-epitope coding sequence into NheI and BamHI. The in vitro translation reporter pFL-SV40 was constructed by replacing the renilla luciferase of pRL-SV40 (Promega, Madison, WI, USA) with the firefly luciferase.

HeLa and HEK293 cells were grown in DMEM (Gibco, Grand Island, NY, USA) supplemented with 10% fetal calf serum and penicillin ⁄ streptomycin. Transfection was performed using Lipofectamine 2000 (Invitrogen) as recommended by the manufacturer. For stress treatment, HeLa cells were cultured in the presence of 0.5 mm arsenite for 1 h. The procedure for indirect immunofluorescence was essentially as described previously [47] . Polyclonal antibody against the HA epitope was from BAbCO (Richmond, CA, USA). Monoclonal anti-FLAG and anti-PABP were from Sigma (St Louis, MO, USA). Fluorescein isothyocyanate and rhodamine conjugated secondary antibodies were from Cappel Laboratories Cochranville, PA, USA. Immunostained cells were visualized with an Axiovert 200 microscope (Carl Zeiss Inc., Oberkochen, Germany).

The His-tagged SARS-CoV N and NDRS proteins were overproduced in E. coli BL21 (DE3). The bacterial lysate was prepared in a buffer containing 50 mm sodium phosphate (pH 8.0), 300 mm NaCl and 6 m urea, and was subsequently passed through His•Bind Resin (Novagen) for purification of His-tagged proteins. Bound proteins were eluted using the above buffer containing 250 mm imidazole. The eluate was dialyzed against a buffer containing 50 mm sodium phosphate (pH 7.4), 100 mm NaCl, 1 mm EDTA and 0.01% NaN 3 . GST and GST-fusion to N, NDRS and all mutant proteins were overproduced in E. coli strain BL21 and purified over glutathione-Sepharose beads (GE Healthcare) as recommended by the manufacturer. Purified GST fusion proteins were dialyzed against buffer D (20 mm Hepes, pH 7.9, 50 mm KC1, 0.5 mm dithiothreitol, 0.2 mm EDTA and 20% glycerol).

For in vivo phosphorylation, 3 · 10 6 transfected HeLa cells expressing FLAG-N or NDRS in a 60 mm diameter plate were incubated in sodium phosphate-deficient DMEM (Invitrogen) supplemented with 0.75 mCi [ 32 P]orthophosphate (Amersham, Little Chalfont, UK) for 2.5 h. FLAGtagged protein was immunoprecipitated from the cell lysates using anti-FLAG M2 agarose (Sigma) in a buffer containing 10 mm sodium phosphate (pH 7.2), 150 mm NaCl, 2 mm EDTA, 1% NP-40 and a mixture of protease inhibitors (Roche, Indianapolis, IN, USA), which was used as recommended by the manufacturer. In vitro phosphorylation of the N protein using recombinant GST-SRPK1 was essentially as described previously [42] ; the reactions contained 5 lm ATP with or without additional 40 nm [k-32 P]ATP. Dephosphorylation was performed using 200 U k-protein phosphatase (New England Biolabs, Beverly, MA, USA).

Purified recombinant GST-NDRS (3 lm) in 20 mm potassium acetate, 5 mm sodium acetate, 2 mm magnesium acetate and 1 mm EGTA was subjected to far-UV CD analysis using a Jasco J-720 spectropolarimeter (Jasco Inc., Easton, MD, USA). The measurement was performed in the range 190-250 nm in a 1 mm path length cuvette at room temperature. The data were recorded at 1 nm intervals.

The approximately 110 nucleotide RNA probe was in vitro transcribed by T7 RNA polymerase using BamHI-digested pET15b-FLAG as template. The RNA was uniformly labeled with [a- 32 protein was incubated with 5 · 10 4 c.p.m. of 32 P-labeled RNA in a 20 lL reaction containing 10 mm Hepes (pH 7.9), 50 lm EDTA, 10% glycerol, 1 mm dithiothreitol, 5 mm MgCl 2 , 0.1 mg of BSA, 2.5 lg of tRNA and 10 U of RNasin (Promega) at 25°C for 15 min. Samples were fractionated on a 6% polyacrylamide nondenaturing gel in 0.5· TBE buffer (45 mm Tris-HCl, 45 mm boric acid, 1 mm EDTA, pH 8.0). Quantification was performed using Typhoon9410 Variable Mode Imager (Amersham).

The crosslinker disuccinimidyl suberate (Sigma) was prepared in N,N-dimethylformamide (Sigma) and used for chemical crosslinking of recombinant His-tagged N protein.

The reaction mixtures contained 0.35 mm phosphorylated or nonphosphorylated N protein and 5 mm crosslinker in the NMR buffer (5 mm Hepes, 100 mm NaCl, 2 mm KCl, 1 mm MgCl 2 , 2 mm CaCl 2 and 0.5 mm EDTA, pH 7.8).

The reaction was performed at 4°C for 1 h and stopped by 100 mm glycine. Proteins were fractionated by SDS ⁄ PAGE and detected by immunoblotting using anti-SARS-CoV N serum (Imgenex, San Diego, CA, USA). Quantification was performed using image j software (National Institutes of Health, Bethesda MD, USA).

The TNT coupled reticulocyte lysate system (Promega) was used for in vitro translation of a firefly luciferase reporter mRNA that contained 68 and 42 nucleotides in the 5¢ and 3¢ UTR, respectively, and was in vitro synthesized by T7 RNA polymerase from the template pFL-SV40. Each 10 lL of translation reaction contained 100 ng of the luciferase mRNA and different amounts of recombinant GST-N or NDRS protein. The resulting luciferase activity was assessed by the luciferase assay system (Promega). To visualize luciferase protein, [ 35 S]methionin was added into the reaction according to the manufacturer's recommendation.

HEK293 cells were transiently transfected with the vector expressing HA-tagged N or NDRS protein. The cell lysate was then prepared in 10 mm Tris-HCl (pH 7.4), 150 mm NaCl and 3 mm MgCl 2 for sucrose gradient or in 20 mm Hepes (pH 7.9), 100 mm KCl and 1 mm MgCl 2 for glycerol gradient; both buffers additionally contained 100 lgAEmL )1 cycloheximide, 35 lgAEmL )1 digitonin and 20 UAEmL )1 RNasin (Promega). Density gradient sedimentation was performed in a Beckman SW41 rotor (Beckman-Coulter, Fullerton, CA, USA) at 4°C; for sucrose and glycerol gradient sedimentation, the centrifugation condition was 30 000 g for 5 h and 74 000 g for 16 h, respectively.

Proteins were precipitated by 20% trichloroacetic acid from each fraction and analyzed by immunoblotting using anti-SARS-CoV N serum.

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We thank Tai-Huang Huang and Konan Peck for the SARS-CoV N protein cDNAs, plasmids and recombinant proteins, and Chwan-Deng Hsiao and Yi-Wei Chang for CD analysis. We thank Ru-Inn Lin and Wei-Lun Chang for their initial experimental assistance and Dr Tim C. Taylor for editing the manuscript. This work was supported by the National Science Council of Taiwan (NSC 95-3112-B001-007).

The following supplementary material is available online: Fig. S1 . The SARS N protein is phosphorylated by SRPK1 but not by Clk1 and PKA.This material is available as part of the online article from http://www.blackwell-synergy.com Please note: Blackwell Publishing is not responsible for the content or functionality of any supplementary materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.