key: cord-0855666-10w1uvf7
authors: Lei, Yake; Song, Yinggai; Shu, Yilin; Zhao, Youyun; Huo, Xixiang; Wang, He; Zeng, Yingchun; Yu, Xiao; Li, Xiang; Ye, Guojun; Fang, Bin; Han, Shi; Li, Ruoyu; Liu, Linlin
title: Fungal antigenemia in patients with severe Coronavirus Disease 2019 (COVID-19): the facts and challenges
date: 2020-05-25
journal: J Microbiol Immunol Infect
DOI: 10.1016/j.jmii.2020.05.010
sha: dafcc6fa5516c8a161c72bdc8ed4c36c0ea84707
doc_id: 855666
cord_uid: 10w1uvf7

nan

Yake Lei 1 *,Yinggai Song 3,4,5,6 *,Yilin Shu 1 *,Youyun Zhao 2 *, Xixiang Huo 1 *, He Wang 7 , Yingchun Zeng 1 , Xiao Yu 1 , Xiang Li 1 , Guojun Ye 1 , Bin Fang 1 , Shi Han 1 , Ruoyu Li 3,4,5,6 #, Linlin Liu 1 # The traditional detection methods used for fungal infection, such as culture methods 10 and histopathology, might bring unpredictable biosafety issues, since the relevant 11 specimens cannot be inactivated. Therefore, serological assays for fungal antigens, Candida and Aspergillus infection is 70% -80%, and the specificity is 70%-80%. The 15 serum GM detection is as sensitive as 70% -80%. The sensitivity of mannan detection is 16 58% and the specificity is 93%. When the combined detection of mannan antigen and 17 antibody can improve the sensitivity of 83% and the specificity of 86%. 3 18

In this study, we aimed to illustrate the existence of these fungal antigens from 181 Workstation (A200). Rank and sum tests were performed, and count data were analyzed 36 with a χ² test. Differences were considered statistically significant at p < 0.05. There was 37 no significant difference in the mean values and positive rates of G, GM, and Mn between 38 the early-, middle-, and late-stage COVID-19 patient samples (P > 0.05) ( Table 1) . 39

However, the positive GM test rate tended to increase with age, i.e., the positive rate of 40 the groups of patients 50-64 years old and aged ≥50 years old were higher than that of 41 the group of patients aged 20-49 years old. 42

The main limitation of this retrospective study using the residual sera for analysis is 43 the lack of clinical evaluation or other diagnostic approaches for possible fungal infection The authors declare that they have no competing interests. 

A national survey 63 on fungal infection diagnostic capacity in the clinical mycology laboratories of 64 tertiary care hospitals in China

66 guidelines for the use of antifungal agents in patients with invasive fungal 67 diseases in Taiwan

Invasive candidiasis: A review of nonculture-based 69 laboratory diagnostic methods

Epidemiological and 71 clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in 72

China: a descriptive study