key: cord-0851920-3463r8qy
authors: Garfield, Benjamin; McFadyen, Charles; Briar, Charlotte; Bleakley, Caroline; Vlachou, Aikaterini; Baldwin, Melissa; Lees, Nick; Price, Susanna; Ledot, Stephane; McCabe, Colm; Wort, S John; Patel, Brijesh; Price, Laura C.
title: Potential for personalised application of inhaled nitric oxide in COVID-19 pneumonia
date: 2020-11-14
journal: Br J Anaesth
DOI: 10.1016/j.bja.2020.11.006
sha: 1445bd017c7304723e4fb360c2a1dd9d9ad76456
doc_id: 851920
cord_uid: 3463r8qy

nan

Editor -Coronavirus disease 2019 (COVID-19) has affected 31.1 million people worldwide and caused over 1.2 million deaths as of November 2020. 1 The majority of critical care patients with COVID-19 pneumonia fulfil the Berlin definition of acute respiratory distress syndrome (ARDS) requiring invasive mechanical ventilation. 2 Despite standard ARDS interventions with high positive endexpiratory pressure, prone positioning and lung protective ventilation, some patients remain profoundly hypoxaemic and hypercapnic. 2 Inhaled nitric oxide (iNO) has been a rescue strategy used previously in ARDS, where it increases PaO 2 /FiO 2 ratio and reduces physiologic dead space fraction at 24 h, but without improving mortality or intensive care unit (ICU) length of stay. 3 A dual role for iNO has been proposed in COVID-19 as an anti-viral agent and as a pulmonary vasodilator, especially given the 'pulmonary vascular' phenotype increasingly apparent in this disease. 4 5 Whether patients with COVID-19 are 'responders' to iNO, and factors that predict potential responsiveness, remain unknown. A recent publication in this journal 6 described an observational series of 16 patients with COVID-19 related ARDS, in whom iNO was employed as a rescue therapy for refractory hypoxaemia. The authors report a lesser improvement in PaO 2 /FiO 2 ratio following iNO initiation than in a historical (non-COVID-19) ARDS comparison cohort from their institution. They were unable to delineate any biochemical or physiological characteristics predictive of a positive response to iNO in COVID-19 ARDS patients. For comparison, we present our experience at one of the five Severe Acute Respiratory Failure centres in the UK. We report the effects of iNO in a population of patients with COVID-19-induced ARDS with refractory hypoxaemia and evaluate the predictors of iNO responsiveness in these patients.

ICU patients admitted to a tertiary respiratory failure centre in the UK receiving iNO with at least moderate ARDS (PaO 2 /FiO 2 ratio < 26.7 mm Hg/3.56 kPa) 7 between March 2020 and May 2020 were included in this observational study. Data were collected on patient chracteristics, respiratory physiology, baseline blood tests and the use of ARDS interventions during the study period. Oxygenation and carbon dioxide clearance were evaluated before and in the first 24 h after initiating iNO. Measurements were taken as an average of three values at each time point from stored ventilator data to calculate PaO 2 /FiO 2 ratio, oxygenation index (OI) 8 and dead space fraction based on the Engelhoff modification of the Bohr equation. 9 In patients who received prone positioning, follow-up measurements were taken in the same patient position. Responders to iNO were defined at those exhibiting an increase in PaO 2 /FiO 2 ratio of >1.33 kPa at 24 h. 10 Change in PaO 2 /FiO 2 ratio, OI and dead space fraction at 3 and 5 days, and in the 24 h before and after stopping iNO were also examined. Outcomes including length of stay and 30-day mortality were analysed.

Data are presented as mean (standard deviation) or median (interquartile range). Statistical analysis used Graphpad prism (v. 5; GraphPad, San Diego, CA, USA). Ethical approval for analysis of retrospective data was in place (A-CLUE 285452, IRAS Reference: 285452) through the Royal Brompton and Harefield Research Ethics Committee. All patients lacked capacity, and the need for individual informed consent was waived for retrospective analysis of data collected prospectively for routine care, without breach of privacy or anonymity.

Thirty-five consecutive patients (20% female) were included, with a mean age of 57.6 (8.1) yr and mean body mass index 30.8 (5.3) kg m -2 , with 32 (91.4%) patients undergoing proning at the time of initiation of nitric oxide. Patients who were not being proned had right ventricular (RV) failure (1), recent cardiac arrest (1) or morbid obesity (1). Two of these patients required proning at a later date. Patients were treated with 20 ppm iNO for an average of 146.4 (80.8) h, with the exception of one patient treated with 40 ppm.

Within 24 h of iNO initiation, there was a significant increase in PaO 2 /FiO 2 ratio from baseline (13.6 (3.9) vs. 17.4 (5.5) kPa, p<0.001) (Figure 1A) , a reduction in OI (20.6 (15.2-24.0) vs. 14.4 (11.9-20.8), p<0.001) and a reduction in dead space fraction (0.28 (0.10) vs 0.24 (0.09), p=0.038) (Figure 1B) . The change in PaO 2 /FiO 2 ratio and OI (data not shown), but not in dead space fraction, was preserved in those who survived to 48 h and 5 days. Compared to the immediate values before weaning, when stopping iNO there was no significant change in PaO 2 /FiO 2 ratio or dead space fraction (Figure1 A  and B) .

According to the pre-defined improvement in PaO 2 /FiO 2 ratio by at least 1.33 kPa, 23 patients (65.7%) responded to iNO at 24 h. Responders had a significantly lower baseline PaO 2 /FiO 2 ratio (12.1 (2.8) vs. 16.3 (4.4) kPa, p<0.01) and higher baseline OI (21.6 (6.3) vs. 16.1 (5.2), p<0.01) than non-responders. Responders to iNO also had higher baseline brain natriuretic peptide (BNP) (available in n=16) (187 (84-529) ng L -1 vs 43 (34-112) ng L -1 , p=0.023). Baseline high sensitivity (hs)troponin levels (available in n=33) taken prior to initiation of iNO were not different between the groups overall ( Table 1 ). Taking twice the upper limit of our hs-troponin assay (>27 ng L -1 ), patients in the non-responder group all fell below this cut-off, compared to higher hs-troponin levels in responders (Fisher's exact test p=0.015). Of the 35 patients studied, 2 later required extra-corporeal membrane oxygenation, both at 3 days after iNO initiation. The 30-day mortality after starting iNO was 17/35 (48.5%). This is the third report of the use of iNO in patients with ARDS caused by COVID-19 6 11 , and the largest cohort reported to date. In contrast to these previous reports, we observed that in patients with at least moderately severe ARDS, iNO at 20 ppm improved oxygenation (PaO 2 /FiO 2 ratio and OI) and ventilatory efficiency (dead space fraction), with 65.7% of patients responding to iNO at 24 h. We were also able to differentiate responders from non-responders on the basis of higher levels of the cardiac biomarkers BNP and hs-troponin. More responders had severe ARDS (17/23 responders vs 3/12 non-responders).

Several factors may account for differences between our findings, and those of Longobardo and colleagues 6 , who reported only minimal improvement in PaO 2 /FiO 2 ratio with iNO, and did not identify any patient characteristics associated with response to therapy. We routinely collect hstroponin on all COVID-19 patients admitted to our institution. We used a dose of 20 ppm in all patients except one (who received 40 ppm), whereas the Longobardo cohort received 10-20 ppm. Finally, our cohort was enriched for patients with right ventricular dysfunction or pulmonary hypertension, with 23/35 patients having one or both on transthoracic echocardiography prior to iNO initiation. The use of iNO is widespread in the management of oxygenation and pulmonary hypertension during lung or heart transplantation, and as a treatment for acute RV failure following cardiotomy and cardiopulmonary bypass 12 13 . It is plausible that ARDS patients with these characteristics would be more likely to derive benefit from iNO therapy.

iNO has been shown to improve oxygenation and reduce dead space ventilation in ARDS. 3 Our data support its use as a rescue therapy in COVID-19 ARDS refractory to standard management including proning. Whether iNO confers a mortality benefit in patients with COVID-19 pneumonia remains to be seen, but the potential disease modifying effect 4 make it an attractive intervention for further study. Reassuringly in this small cohort on a variety of other treatments there was no evidence of rebound hypoxaemia or hypercapnia after cessation of iNO therapy.

These data suggest the iNO may be beneficial in those patients with more severe hypoxaemia with raised BNP and hs-troponin, likely suggestive of RV strain. It is also likely that these patients have a pulmonary vascular phenotype, increasingly recognised in COVID-19 pneumonia. 5 This specific phenotypic response to iNO support the interplay between pulmonary vascular blood flow and right heart function in COVID-19 pneumonia, with RV dysfunction increasingly reported, 14 and associated with radiological signs suggesting pulmonary microthrombosis in severe cases. 15 iNO may be helpful in patients with COVID-19 with refractory hypoxaemia despite standard interventions, especially in those with raised BNP and troponin. BNP and hs-troponin may be useful biomarkers for phenotypic enrichment for future clinical trials of iNO in COVID-19 ARDS. This highlights the possibility for personalised application of iNO in clinical trials that utilise biomarkers and echocardiography findings for phenotypic enrichment.

BG, BP, SL, SJW and LP substantially contributed to conception and design and revising it critically for important intellectual content. CB, AV, CM and MB acquired data. BG and LP analysed and interpreted the data, and drafted the article. All authors contributed to, revised and approved the final manuscript.

BP declares personal fees from GSK and grants from Mermaid Care A/C, ESICM, RBHT charity, European Commission, Academy of Medical Sciences and Imperial College London COVID fund. SJW declares grants and personal fees from Actelion and Bayer and personal fees from GSK and MSD. LP declares personal fees from Actelion. The other authors declare no conflict of interest with regard to the production or submission of this manuscript.

This retrospective project received no specific funding

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