key: cord-0851843-uqprwtvn authors: So, Daeyoung; Min, Kyueng-Whan; Jung, Woon Yong; Han, Sang-Woong; Yu, Mi-Yeon title: Microscopic Polyangiitis Following mRNA COVID-19 Vaccination: A Case Report date: 2022-05-09 journal: J Korean Med Sci DOI: 10.3346/jkms.2022.37.e154 sha: 93b47bdfa05cec14399e30bad702c8b128e5f017 doc_id: 851843 cord_uid: uqprwtvn Coronavirus disease 2019 (COVID-19) is one of the most widespread viral infections in human history. As a breakthrough against infection, vaccines have been developed to achieve herd immunity. Here, we report the first case of microscopic polyangiitis (MPA) following BNT162b2 vaccination in Korea. A 42-year-old man presented to the emergency room with general weakness, dyspnea, and edema after the second BNT162b2 vaccination. He had no medical history other than being treated for tuberculosis last year. Although his renal function was normal at last year, acute kidney injury was confirmed at the time of admission to the emergency room. His serum creatinine was 3.05 mg/dL. Routine urinalysis revealed proteinuria (3+) and hematuria. When additional tests were performed for suspected glomerulonephritis, the elevation of myeloperoxidase (MPO) antibody (38.6 IU/mL) was confirmed. Renal biopsy confirmed pauci-immune anti-neutrophil cytoplasmic antibody (ANCA)-related glomerulonephritis and MPA was diagnosed finally. As an induction therapy, a combination of glucocorticoid and rituximab was administered, and plasmapheresis was performed twice. He was discharged after the induction therapy and admitted to the outpatient clinic 34 days after induction therapy. During outpatient examination, his renal function had improved with serum creatinine 1.51 mg/dL. We suggest that MPA needs to be considered if patients have acute kidney injury, proteinuria, and hematuria after vaccination. Coronavirus disease 2019 (COVID-19) is one of the deadliest and most widespread viral infections in human history. Currently, there are approximately 200 million confirmed cases and five million deaths worldwide. 1 COVID-19 is caused by an enveloped positive-stranded RNA virus that includes beta-coronavirus with the same subgenus but a different strain as severe acute respiratory syndrome coronavirus, which caused a pandemic in 2003. 2 As a result of increased globalisation, infectious diseases can be transmitted much more efficiently. 3 Owing to the highly contagious nature of COVID-19, vaccination is important to achieve herd immunity and prevent disease outbreaks. BNT162b2 and mRNA-1273 are representative mRNA vaccines, and their safety has been proven through several clinical trials worldwide. Research on the efficacy and safety of vaccines is still in progress. 4-7 The shortterm adverse effects of the vaccine are not significantly different from those of other vaccines, and long-term adverse effects are not yet known. Moreover, in patients with rare adverse effects, it is difficult to evaluate the causal relationship with the vaccine. There had been several case reports of anti-neutrophil cytoplasmic antibody (ANCA)associated vasculitis (AAV) occurring after vaccination. [8] [9] [10] [11] [12] Recently, several cases of AAV were reported worldwide after receiving the COVID-19 mRNA vaccine. 13-17 Here, we report the first case of MPA following the second dose of BNT162b2 vaccine in Korea. A 42-year-old man visited the emergency department because of general weakness, shortness of breath, edema, gross hematuria, and significant weight loss (5 kg) that had developed approximately 2-3 weeks earlier. The symptoms occurred after receiving a second dose of BNT162b2 vaccine on October 5, 2021, 63 days after the first dose. He had not taken any medication earlier except tuberculosis medication between August 2020 and March 2021. In addition, there were no specific findings in the family history and psychosocial history. On physical examination, the initial vital signs were stable, and mild pitting edema was seen in both legs. Any other signs, such as arthralgia, redness, sensation of heat on the skin, and skin rash were not observed. Routine laboratory assessments obtained 201 days prior to receiving a second dose vaccine were normal for serum creatinine (sCr) of 0.97 mg/dL (reference range: 0.5-0.10) and estimated glomerular filtration rate (eGFR) of 96.6 mL/min/1.73 m 2 (reference range: 60-180) ( Table 1 ). The serum levels of acute phase reactants were normal for white blood cell (WBC) was 7,700 /mm 3 and C-reactive protein was 0.7 mg/dL. Acute kidney injury (AKI) at admission was observed with sCr levels of 3.05 mg/dL, blood urea nitrogen (BUN) of 59 mg/dL (reference range: 8.0-21) and an eGFR of 24.0 mL/min/1.73 m 2 ( Table 1) . Dipstick urine test showed proteinuria (3+), hematuria (many red blood cells under high-power field [HPF] , and epithelial cells [0-4 under HPF]) but was negative for glucose, WBCs, bacteria, and hyaline casts. The random urinary protein-creatinine ratio (UPCR) was 3,438 mg/g*Cr. The following tests were found to be within the normal range: Complement component 3 Anti-proteinase-3 antibodies were not determined, whereas MPO antibodies were elevated at 38.6 IU/mL (reference range: < 3.5). A renal biopsy was performed 29 days after the second vaccination. On histological examination, 21 glomeruli were identified, among which nine cellular crescents and two fibrous crescents were identified. Diffuse necrotising and crescentic glomerulonephritis with mild interstitial inflammation was confirmed using a light microscope (Fig. 1) . In addition, a paucity of immune deposits was confirmed by immunofluorescence microscopy. According to histological findings, pauci-immune ANCArelated glomerulonephritis was confirmed, and finally, MPA was diagnosed. Combination therapy with glucocorticoid and rituximab was administered as induction therapy, and plasma exchange was additionally performed. Methylprednisolone (500 mg) was administered intravenously for three days, and thereafter, oral prednisolone (1 mg/kg) was administered. A bolus of 622 mg of rituximab was administered intravenously for 4 weeks (375 mg/m 2 every week). Plasma exchange was performed twice because of the patient's reluctance. He was discharged on November 16, 2021, 19 days after induction therapy. On November 30, 2021, the patient visited the outpatient clinic, 33 days after induction therapy. He said that general weakness still remained mild, but other symptoms were improved. Actually, kidney function was partially restored (Table 1 and Fig. 2) . Nephrotic-range proteinuria (random UPCR, 4,203 mg/g*Cr) was still observed, but anti-MPO antibodies decreased to 19.6 IU/mL. There were no side effects. The current treatment was effective, and no side effects were found, so we decided to follow up with him as an outpatient and reduce the steroid dose. We reported that MPA occurred in a healthy man after the administration of the BNT162b2 vaccine in Korea, firstly. It is accepted that the COVID-19 mRNA vaccine is safe and effective, although the COVID-19 vaccine has been used only for approximately 2 years. However, serious adverse events are rarely reported as vaccination rates increase. Several cases of MPA after COVID-19 mRNA vaccine administration have been reported, and MPA occurred after the second dose of vaccine. 13-16 Hematuria and proteinuria, which are representative of kidney involvement, were also observed. Except for one patient with normal kidney function at diagnosis of MPA, 16 three patients with AKI recovered kidney function after immunosuppressive treatment. 13-15 Similar to previous reports, MPA in our case occurred after the second dose of vaccine and is currently improving with rituximab and steroid treatment. In influenza immunization, which is an mRNA vaccine like BNT162b2 vaccine, there have been several cases of AAV following vaccination, and a causal relationship and its mechanism have been studied. [8] [9] [10] [11] It is hypothesised that the molecular similarity between vaccine components and antigens on the surface of human neutrophils after vaccination induces the production of pathogenic ANCA or hypersensitivity of the vaccine to viral RNA induces the production of ANCA. 9,18 AAV may have occurred after COVID-19 mRNA vaccination by a mechanism similar to AAV following other vaccinations. Enhanced immune response after the second vaccination could promote pathogenic ANCA, eventually leading to AAV. However, the exact mechanism by which COVID-19 mRNA vaccines induce AAV other than through temporal causality has not yet been elucidated. MPA could be also a random event unrelated to vaccination. Additional causality studies are required to establish safer vaccination policies. In AAV, the patient mortality rate was 19-50% at 5 years, and kidney survival rates at 5 years were 70-80%, depending on the severity of kidney involvement at diagnosis. 19 produced a positive effect in 70-80% of patients with ANCA-associated rapid progressive glomerulonephritis. 22 Crescent glomerulonephritis was confirmed in five patients with AAV following COVID-19 mRNA vaccination in previous studies, including our report. In all patients, renal function was restored. Although few cases have been reported, the prognosis for vaccine-associated AAV may be better than that for AAV caused by other causes. Because of several case reports of AAV onset following COVID-19 vaccination, we may have reasonable suspicion and bring forward the diagnosis and treatment of rare AAV following COVID-19 vaccination. Currently, COVID-19 vaccines are being considered for an annual booster shot, such as seasonal boosters for flu and respiratory syncytial viruses. We suggest that MPA needs to be considered if patients have acute kidney injury, proteinuria, and hematuria after vaccination. COVID-19) dashboard Coronaviridae Study Group of the International Committee on Taxonomy of Viruses. 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And, we also thank Editage for English language editing.