key: cord-0851180-hyxek4os
authors: Stoeckle, Kate; Witting, Britta; Kapadia, Shashi; An, Anjile; Marks, Kristen
title: Elevated inflammatory markers are associated with poor outcomes in COVID‐19 patients treated with remdesivir
date: 2021-08-23
journal: J Med Virol
DOI: 10.1002/jmv.27280
sha: 5168a2385ecaa6bf13a57ee62b83ff9ab4bb284a
doc_id: 851180
cord_uid: hyxek4os

The antiviral remdesivir has been shown to decrease the length of hospital stay in coronavirus disease 2019 (COVID‐19) patients requiring supplemental oxygen. However many patients decompensate despite being treated with remdesivir. To identify potential prognostic factors in remdesivir‐treated patients, we performed a retrospective cohort study of patients hospitalized at NewYork‐Presbyterian Hospital/Weill Cornell Medical Center between March 23, 2020 and May 27, 2020. We identified 55 patients who were treated with remdesivir for COVID‐19 and analyzed inflammatory markers and clinical outcomes. C‐reactive protein (CRP), d‐dimer, and lactate dehydrogenase levels were significantly higher in patients who progressed to intubation or death by 14 days compared to those who remained stable. CRP levels decreased significantly after remdesivir administration in patients who remained nonintubated over the study period. To our knowledge, this is the largest study to date examining inflammatory markers before and after remdesivir administration. Our findings support further investigation into COVID‐19 treatment strategies that modify the inflammatory response.

of Phase 3 randomized, open-label, multicenter study of remdesivir therapy in patients with severe COVID-19 (NCT04292899). Severe COVID-19 was defined as an oxygen saturation of less than or equal to 94% on room air or requirement of supplemental oxygen. For our study, we excluded patients who were mechanically ventilated at the time of remdesivir administration (N = 20), patients who received tocilizumab or eculizumab (N = 4) and patients who received less than three doses of remdesivir (N = 2). Patients who also received hydroxychloroquine before remdesivir were included as this was local standard of care at the time of the study (N = 29). Patients who received corticosteroids were also included (N = 27). Our primary outcome variable was whether patients were on mechanical ventilation or deceased by Day 14 (progressors) or remained alive and nonintubated (nonprogressors). We used the electronic medical record to obtain levels of inflammatory markers including D-dimer, IL-6, CRP, and ferritin from 4 days before remdesivir administration to 14 days after. Day 1 was considered the first day of remdesivir administration. We performed descriptive statistics using Fisher's exact test and 

Of the 55 patients included, 9 were progressors and 46 were nonprogressors. The median age was 66 in progressors and 62 in nonprogressors, and 36% overall were women. The median CRP throughout the study period was significantly higher in progressors compared to nonprogressors (24 vs. 9 mg/L; p < 0.001), as shown in Table 1 . The median D-dimer and lactate dehydrogenase were also higher in progressors than in nonprogressors (871 vs. 576 ng/ml; p = 0.002, 511 vs. 392 U/L; p < 0.001, respectively). Median levels of ferritin and IL-6 were higher in progressors compared to nonprogressors however the trend did not reach significance (1225 vs. 1033 ng/ml; p = 0.17, 32 vs. 18 pg/ml; p = 0.39, respectively).

In the mixed-effect analysis, we found that patients ontreatment (β = −4.43, p = 0.003) and posttreatment (β = −6.94, p < 0.001) had significantly lower median CRP compared to pretreatment levels. Adjusting for the time period, nonprogressors had significantly lower median CRP compared to progressors (β = −10.01, p < 0.001).

After adding the two-way interaction for time and progressor status, results showed that median CRP between the two groups ontreatment compared to pretreatment CRP was significantly different (β = −7.87, p = 0.027). At posttreatment (compared to pretreatment), nonprogressors had significantly decreased CRP compared to progressors (β = −9.76, p = 0.008; Figure 1 ).

In this observational study, we describe inflammatory markers in patients hospitalized with COVID-19 and treated with remdesivir. To our knowledge, ours is the largest study to date examining inflammatory markers before and after remdesivir administration. We found that median CRP, D-dimer, and LDH levels were higher in patients who progressed to intubation or death by 14 days compared to those who did not progress. We found that the median ferritin and IL-6 levels were also higher in progressors, however, the differences were not significant. It is possible that this is because there were fewer recorded values for ferritin and IL-6 compared to the other inflammatory markers. Our data are consistent with prior studies and suggest that elevated CRP, D-dimer, and LDH levels can be used as predictors of poor clinical outcomes including mechanical ventilation and death in patients with severe COVID-19 pneumonia. Many of these studies however do not disclose which treatment, if any, their patients were receiving. [5] [6] [7] [8] Our study demonstrates that these markers can be reliable reflections of clinical status in patients treated with remdesivir.

We found that CRP levels decreased significantly after remdesivir administration in nonprogressors compared to progressors. Our results are consistent with the hypothesis that remdesivir attenuates the inflammatory response in a certain subset of patients but not in all. This is in line with several studies showing that COVID-19 patients treated with a 5 or 10 days course of remdesivir had a shorter time to recovery than those who received placebo. 9-11 Current recommendations from T A B L E 1 Demographics and inflammatory markers in progressors and nonprogressors the Infectious Diseases Society of America and the National Institutes of Health advise treatment with remdesivir in hospitalized patients with COVID-19 who require supplemental oxygen. 12, 13 Of note, the World Health Organization recommends against remdesivir in light of their Solidarity trial findings that it had no effect on mortality rates or duration of hospital stay in COVID-19 patients. 14 In addition a recent large cohort study found that remdesivir was associated with longer hospital stays and had no survival benefit. 15 Further work is needed to understand the link between antiviral activity and the subsequent inflammatory response. However because we did not have a control group, it is unclear whether the differences we saw between progressors and nonprogressors were due to remdesivir, the natural course of disease, or another factor, such as corticosteroids, which many of our study patients received (78% of progressors and 38% of nonprogressors). Future studies examining other inflammatory markers including erythrocyte sedimentation rate, fibrinogen, and complement levels may give further insight into prognostic indicators and potential therapeutic targets. 16, 17 

In sum, we found that inflammatory markers were higher in COVID-19 patients treated with remdesivir who had poor clinical outcomes compared to patients who remained stable. In addition, CRP levels decreased significantly after remdesivir administration in patients who remained nonintubated over the study period. Our findings support further investigation into COVID-19 treatment strategies that modify the inflammatory response.

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Elevated inflammatory markers are associated with poor outcomes in COVID-19 patients treated with remdesivir

The authors thank the participants and study staff for their dedication to the study. Research grant paid to the institution from Gilead Sciences Inc., for the conduct of Phase 3 remdesivir clinical trial (NCT04292899). Anjile An was partially supported by the Clinical and