key: cord-0845098-9ahbqtcq authors: Pogorelić, Zenon; Anand, Sachit; Žuvela, Tomislav; Singh, Apoorv; Križanac, Zvonimir; Krishnan, Nellai title: Incidence of Complicated Appendicitis during the COVID-19 Pandemic versus the Pre-Pandemic Period: A Systematic Review and Meta-Analysis of 2782 Pediatric Appendectomies date: 2022-01-06 journal: Diagnostics (Basel) DOI: 10.3390/diagnostics12010127 sha: e7a41b44933e9cd2896e43bd26c2f846356a028e doc_id: 845098 cord_uid: 9ahbqtcq Background: The Coronavirus Disease 2019 (COVID-19) pandemic has impacted volume, management strategies and patient outcomes of acute appendicitis. The aim of this systematic review and meta-analysis was to evaluate whether the COVID-19 pandemic resulted in higher incidence of complicated appendicitis in children presenting with acute appendicitis compared to the pre-COVID-19 period. The secondary aim was to investigate the proportion of the patients treated by non-operative management (NOM). Methods: A systematic search of four scientific databases was performed. The search terms used were (coronavirus OR SARS-CoV-2 OR COVID-19 OR novel coronavirus) AND (appendicitis). The inclusion criteria were all patients aged <18 years and diagnosed with acute appendicitis during the COVID-19 and pre-COVID-19 periods. The proportion of children presenting with complicated appendicitis and the proportion of children managed by NOM was compared between the two groups. The Downs and Black scale was used for methodological quality assessment. Results: The present meta-analysis included thirteen studies (twelve retrospective studies and one cross-sectional study). A total of 2782 patients (1239 during the COVID-19 period) were included. A significantly higher incidence of complicated appendicitis (RR = 1.63, 95% CI 1.33–2.01, p < 0.00001) and a significantly higher proportion of children managed via the NOM (RR = 1.95, 95% CI 1.45–2.61, p < 0.00001) was observed in patients during the COVID-19 pandemic when compared to the pre-COVID-19 period. Conclusion: There is a significantly higher incidence of complicated appendicitis in children during the COVID-19 pandemic than in the pre-COVID-19 period. Additionally, a significantly higher proportion of children was managed via the NOM during the pandemic in comparison to the pre-pandemic period. Acute appendicitis is the most common condition in the pediatric population that leads to emergency abdominal surgery [1, 2] . Although advanced diagnostic imaging is widely available, the initial diagnosis of appendicitis in children can be challenging, with rates of misdiagnosis reaching 100% in children aged two years or younger [3] [4] [5] [6] . This has been attributed to nonspecific presentation and overlap of symptoms with other common Diagnostics 2022, 12, 127 2 of 13 childhood conditions such as mesenteric lymphadenitis, gastroenteritis, or Meckel's diverticulitis. Clinical scores, such as Alvarado, appendicitis inflammatory response score, and pediatric appendicitis score have been developed to aid the diagnosis of acute appendicitis in children [3, 4] . The diagnostic delay often leads to a higher incidence of complications, such as perforation. Perforation rates show an inverse relation to age, ranging from 47.3% in children five years of age, to 100% in children under two years of age [5, 6] . Elevated inflammatory markers from blood or even hyponatremia and hyperbilirubinemia have been shown to assist in distinguishing between simple and perforated appendicitis [7] [8] [9] . Despite advances in medicine, especially in imaging diagnostics, acute appendicitis still in a certain percentage of patients remains unrecognized and mistreated in the initial stage of the disease [1, 8, 9] . In addition to all diagnostic challenges, the Coronavirus Disease 2019 (COVID-19) pandemic has become a new obstacle to overcome. The pandemic has disrupted the normal practice of economy, governance, and scientific and medical expertise [10] . Confinement measures, introduced in order to minimize the number of infected people, have had an impact on patients, medical procedures, and healthcare workers [11] . Many governing bodies have recommended the cancellation of elective surgical procedures during the pandemic, resulting in a major burden on healthcare systems [12] . A decline in admission rates for numerous medical and surgical conditions has been observed, possibly due to a generalized public fear of presenting to a hospital during the pandemic [13] [14] [15] [16] [17] . Despite the confinement measures, acute appendicitis does not quarantine [18] . The pandemic has impacted volume, diagnostic and management strategies, and patient outcomes of acute appendicitis [19, 20] . A nationwide study in the United States found a significant decrease in acute appendicitis presentation, while two studies from Germany observed a decrease in the number of appendectomies during the lockdown [19, 21, 22] . Additionally, it was suggested that non-operative management (NOM) could be a safe alternative to surgery during the pandemic [23] [24] [25] . A study from Budapest suggests that a higher number of perforated appendices is in line with international trends, and shares no correlation with the COVID-19 pandemic [24] . In contrast, various studies have also demonstrated no significant differences in the rates of complicated appendicitis among children presenting during the pandemic versus the pre-pandemic period [19, 20] . Due to these conflicting findings, there is no consensus statement regarding the incidence of complicated appendicitis among the children presenting during the pandemic. The aim of this study was to systematically summarize and compare all relevant data on pediatric complicated appendicitis during the pandemic and pre-pandemic periods. A secondary aim was to investigate the proportion of the patients treated by non-operative management (NOM). We hypothesize that the incidence of complicated appendicitis is higher in children presenting during the COVID-19 pandemic. The present review was not registered in any prospective register or database. The systematic search was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines [26] . A pilot literature search in the PubMed database was independently performed by two authors (SA and NK) on 14 October 2021, to confirm the absence of any systematic reviews on this subject. On the same day, both the authors systematically explored the PubMed, EMBASE, Web of Science, and Scopus databases (Appendix A, Table A1 ) using the following keywords: (Coronavirus OR SARS-CoV-2 OR COVID-19 OR novel Coronavirus) AND (appendicitis). Search filters were used to identify the studies in the pediatric population (Appendix A). Following identification of the total records and duplication removal, the remaining articles were screened as per the inclusion/exclusion criteria to select the relevant ones. The inclusion criteria in the present systematic review were: Participants-All patients aged <18 years and diagnosed with acute appendicitis (clinico-radiologic criteria) during the COVID-19 pandemic; Intervention-appendectomy or conservative management; Comparison-patients with acute appendicitis presenting during the pre-pandemic period (similar months of the previous year); Outcomes-the proportion of children presenting with complicated appendicitis (perforation of appendix, or gangrenous appendix, or abscess/phlegmon) was the primary outcome studied in this review. The proportion of children managed by NOM was the secondary outcome. All comparative studies reporting the primary outcome were eligible for inclusion in the meta-analysis. The type of operative approach, whether laparoscopic or open appendectomy, was not considered a specific eligibility criterion in this review. During the process of selection of the studies for meta-analysis, articles in non-English language were translated using Google translate feature of the Google search engine. Studies with unavailable full-texts were excluded. In addition, case reports, case series, expert opinions, editorials, review articles, and commentaries were also excluded. Data extraction was independently performed by two authors (T.Ž. and Z.K.) utilizing Microsoft Excel spreadsheets (Version 15.24). Data including the total sample size, the number of patients in each treatment group, the average duration of symptoms, the proportion of children with complicated appendicitis, the proportion of children managed by the NOM, and the average length of hospital stay (LOS) were recorded. In addition, the bibliographic information of each included study, i.e., the name of the first author, year of publication, and the type of the study design were also recorded. Any disagreements among the authors were resolved by discussion with the senior author (Z.P.). Two authors (S.A. and A.S.) independently performed the methodological quality assessment utilizing the Downs and Black scale [27] . This twenty-seven-item scale has minimum and maximum scores of 0 and 32, respectively. On the basis of these scores, the risk of bias in each study was declared as high (score 0-15), moderate (score = 16-23), or low (score > 23). Subsequently, the inter-observer agreement on these scores was adjudged using the Kappa statistics [28] . The level of agreement was defined as almost perfect (0.81-1.00), substantial (0.61-0.80), moderate (0.41-0.60), fair (0.21-0.40), or slight (0.00-0.20). The baseline data were expressed as numbers, proportions, averages (mean or median), and ranges. Meta-analysis was performed using the RevMan 5.4 (Cochrane Collaboration, London, UK) software. As both the outcomes (primary and secondary) were dichotomous, the risk ratios (with 95% CI) were calculated for them. Subsequently, the Mantel-Haenszel method was used to evaluate the pooled risk ratio [29] . Heterogeneity among the included studies was estimated using the I 2 statistics. A random-effects model was selected if the heterogeneity was substantial (I 2 > 50%). A p-value of <0.05 was considered statistically significant. For the purpose of analysis, groups A and B included children presenting during the COVID-19 pandemic and pre-pandemic periods, respectively. A total of 245 articles were identified with our search strategy. Of these, 80 duplicate records were removed. Subsequently, the abstracts of the remaining 165 articles were screened for eligibility ( Figure 1 ). Of these, 134 abstracts were excluded and only 31 articles were eligible for full-text review. Thirteen of these were further excluded as they included only adult patients (n = 10), were letters to the editor (n = 2), or brief communication (n = 1). Although eighteen studies [30] [31] [32] [33] [34] [35] [36] [37] [38] [39] [40] [41] [42] [43] [44] [45] [46] [47] were included for systematic review, only thirteen [30] [31] [32] [33] [34] [35] [36] [37] [38] [39] [40] [41] [42] were selected for the final meta-analysis. The remaining five studies [43] [44] [45] [46] [47] compared the two patient groups from non-identical time periods. A total of 2782 patients, 1239 and 1543 in groups A and B, respectively, were included in the meta-analysis. A total of 245 articles were identified with our search strategy. Of these, 80 duplicat records were removed. Subsequently, the abstracts of the remaining 165 articles wer screened for eligibility ( Figure 1 ). Of these, 134 abstracts were excluded and only 31 art cles were eligible for full-text review. Thirteen of these were further excluded as they in cluded only adult patients (n = 10), were letters to the editor (n = 2), or brief communica tion (n = 1). Although eighteen studies [30] [31] [32] [33] [34] [35] [36] [37] [38] [39] [40] [41] [42] [43] [44] [45] [46] [47] were included for systematic review, onl thirteen [30] [31] [32] [33] [34] [35] [36] [37] [38] [39] [40] [41] [42] were selected for the final meta-analysis. The remaining five studie [43] [44] [45] [46] [47] compared the two patient groups from non-identical time periods. A total of 278 patients, 1239 and 1543 in groups A and B, respectively, were included in the me ta-analysis. The baseline characteristics of the studies included in the meta-analysis are demon strated in Table 1 . All studies, except one, were retrospective in nature. The study b Place et al. was cross-sectional in design [33] . Ten out of thirteen studies compared th average duration of symptoms among the two patient groups. A significant differenc was observed in the four studies only [31, 35, 37, 41] . Similarly, ten studies compared th average LOS among both groups [35, 37, 42] . The baseline characteristics of the studies included in the meta-analysis are demonstrated in Table 1 . All studies, except one, were retrospective in nature. The study by Place et al. was cross-sectional in design [33] . Ten out of thirteen studies compared the average duration of symptoms among the two patient groups. A significant difference was observed in the four studies only [31, 35, 37, 41] . Similarly, ten studies compared the average LOS among both groups [35, 37, 42] . The Downs and Black scoring by both authors is depicted in Table 2 . The average scores ranged between 17.5 to 25. The total scores and inter-observer agreement are preselected in Table 3 . Twelve out of thirteen studies had a moderate risk of bias. The studies by Place et al. [33] and Theodorou et al. [42] had the lowest and the highest scores, respectively. The inter-observer agreement was almost perfect (Kappa = 0.961; p < 0.001) (Figure 2 ) demonstrated a statistically significant difference in the incidence of complicated appendicitis among the children of group A versus group B (RR = 1.63, 95% CI 1.33-2.01, p < 0.00001). For this outcome, the estimated heterogeneity among the included studies was statistically significant (I 2 = 57%, p = 0.005). This outcome was reported by all thirteen studies [30] [31] [32] [33] [34] [35] [36] [37] [38] [39] [40] [41] [42] . A total of 980 out of 2782 children, 523 and 457 belonging to groups A and B, respectively, developed complicated appendicitis. Pooling the data ( Figure 2 ) demonstrated a statistically significant difference in the incidence of complicated appendicitis among the children of group A versus group B (RR = 1.63, 95% CI 1.33-2.01, p < 0.00001). For this outcome, the estimated heterogeneity among the included studies was statistically significant (I 2 = 57%, p = 0.005). Eight studies [31, 33, [37] [38] [39] [40] [41] [42] compared the NOM rates among the children belonging to groups A versus B. Two of these had no patients managed via the NOM in either treatment group. Therefore, this outcome analysis consisted of events from six studies only. A total of 118/911 and 62/1005 children, belonging to groups A and B, respectively, were managed via the NOM. Pooling the data (Figure 3 ) demonstrated a significantly higher proportion of children managed via the NOM during the pandemic versus pre-pandemic period (RR = 1.95, 95% CI 1.45-2.61, p <0.00001). The estimated heterogeneity among the included studies was neither substantial nor statistically significant (p = 0.66; I 2 = 0%) for this outcome. treatment group. Therefore, this outcome analysis consisted of events from six studies only. A total of 118/911 and 62/1005 children, belonging to groups A and B, respectively, were managed via the NOM. Pooling the data (Figure 3 ) demonstrated a significantly higher proportion of children managed via the NOM during the pandemic versus pre-pandemic period (RR = 1.95, 95% CI 1.45-2.61, p <0.00001). The estimated heterogeneity among the included studies was neither substantial nor statistically significant (p = 0.66; I 2 = 0%) for this outcome. The aim of this study was to systematically summarize and measure the effects of the ongoing COVID-19 pandemic on the proportion of children with complicated appendicitis and those managed by NOM. A classification of complicated appendicitis is given when there is evidence of a perforated or gangrenous appendix, an intra-abdominal abscess, or fecal peritonitis, which often results in a longer length of stay and greater rates of morbidity and mortality. Overall, complicated appendicitis is more common in children, with rates as high as 30% [6, 48] . One of the reasons for the higher incidence of complicated appendicitis in young patients is diagnostic delay. The diagnostic delay is partly due to unclear anamnesis and atypical clinical presentations found in young patients. Studies showed that appendicitis is a diagnostic challenge with 7-15% of cases presenting twice to the emergency department before diagnosis, resulting in an increase in the rate of complications [49] [50] [51] . The risk of perforation within 24 h of the onset of symptoms is substantial (7.7%), and it increases in a linear fashion with duration, especially with prehospital delay, moreso than with admitted children [52] . Socioeconomic factors, which are globally worsened by ongoing COVID-19 pandemic, are also important factor in delayed presentation of pediatric patients as seeking medical care is dependent upon parents' knowledge of illness, transportation options, insurance status, and financial wellbeing [53, 54] . Another reason for higher incidence of complicated appendicitis in young patients is misdiagnosis. Misdiagnosis is due to the fact that the classical clinical symptoms and laboratory findings that are common in older pediatric population are missing in the younger [4] . Patient age is tied closely to the stage of acute appendicitis, so the youngest patients present with more advanced stages of disease and are at greater risk of perforation, with recent study showing a significant increase of perforation in relation with age as follows: 100% < 1 year; 100% 1-2 years; 83.3% 2-3 years; 71.4% 3-4 years; 78.6% 4-5 years and 47.3% of 5 years [6] . Studies also demonstrate that using various clinical methods (clini- The aim of this study was to systematically summarize and measure the effects of the ongoing COVID-19 pandemic on the proportion of children with complicated appendicitis and those managed by NOM. A classification of complicated appendicitis is given when there is evidence of a perforated or gangrenous appendix, an intra-abdominal abscess, or fecal peritonitis, which often results in a longer length of stay and greater rates of morbidity and mortality. Overall, complicated appendicitis is more common in children, with rates as high as 30% [6, 48] . One of the reasons for the higher incidence of complicated appendicitis in young patients is diagnostic delay. The diagnostic delay is partly due to unclear anamnesis and atypical clinical presentations found in young patients. Studies showed that appendicitis is a diagnostic challenge with 7-15% of cases presenting twice to the emergency department before diagnosis, resulting in an increase in the rate of complications [49] [50] [51] . The risk of perforation within 24 h of the onset of symptoms is substantial (7.7%), and it increases in a linear fashion with duration, especially with prehospital delay, moreso than with admitted children [52] . Socioeconomic factors, which are globally worsened by ongoing COVID-19 pandemic, are also important factor in delayed presentation of pediatric patients as seeking medical care is dependent upon parents' knowledge of illness, transportation options, insurance status, and financial wellbeing [53, 54] . Another reason for higher incidence of complicated appendicitis in young patients is misdiagnosis. Misdiagnosis is due to the fact that the classical clinical symptoms and laboratory findings that are common in older pediatric population are missing in the younger [4] . Patient age is tied closely to the stage of acute appendicitis, so the youngest patients present with more advanced stages of disease and are at greater risk of perforation, with recent study showing a significant increase of perforation in relation with age as follows: 100% < 1 year; 100% 1-2 years; 83.3% 2-3 years; 71.4% 3-4 years; 78.6% 4-5 years and 47.3% of 5 years [6] . Studies also demonstrate that using various clinical methods (clinical exam, laboratory tests, imaging and clinical scores), the availability of which can be reduced during COVID-19 pandemic, is associated with a reduction in the negative appendectomy rate from 14% to 4%, with a slight reduction in the rate of perforated appendicitis [55] . The most accepted mode of treatment of acute appendicitis is appendectomy following fluid resuscitation, analgesia, and intravenous antibiotics. Laparoscopic appendectomy is the most common surgical option with known benefits of lesser incidence of postoperative ileus, a shorter hospital stays, reduced analgesic requirements, a reduced incidence of wound infection and less risk of subsequent adhesive bowel obstruction [1, [56] [57] [58] [59] . Intraabdominal abscess rates are similar after laparoscopic and open appendectomy and are largely determined by whether the appendix is perforated or not [1, [56] [57] [58] [59] . Another option for treatment is NOM (conservative therapy) which can represent a feasible option for acute appendicitis, although complication-free treatment success rates are higher with surgical treatment [1] . Systematic reviews suggest that NOM with antibiotics may fail during the primary hospitalization in about 8% of cases, and an additional 20% of patients might need a second hospitalization for recurrent appendicitis [60] . All of the aforementioned factors in diagnosis and treatment of pediatric appendicitis are being affected by the ongoing COVID-19 pandemic. Since it started in March of 2020, the COVID-19 pandemic represents significant global health threat, a political challenge and has severely affected human life and welfare [11, 61] . Extensive measures, most significant being lockdowns, have been implemented to lower person-to-person transmission and to stop distribution of virus. In the beginning of the pandemic, lockdowns and "Staying home" were most common means to prevent transmission of the virus. During the COVID-19 pandemic elective surgical procedures were canceled in most centers. Surgical procedures were limited only for the care of urgent surgical patients [12] [13] [14] [15] [16] [17] 62, 63] . These efforts to minimize unnecessary traffic through the healthcare facility resulted in a significant reduction in emergency department patient encounters [61, 63] . Results from our study demonstrate a significantly higher incidence of complicated appendicitis among the children in pandemic group versus non-pandemic group. The same results were reported by nine studies included in the meta-analysis, while another four [32, [40] [41] [42] [43] showed an increase in complicated appendicitis but with no statistical significance. Results from six studies, included in secondary outcome, demonstrate a significantly higher proportion of children managed via the NOM during the pandemic versus pre-pandemic period [31, 33, 37, 39, 41, 42] . A recent study by Lazzerini et al. found that pediatric emergency department visits in Italy were reduced by as much as 88% during the peak of their COVID-19 outbreak, in comparison with pre-pandemic years [64] . Significantly higher complicated appendicitis rates during the pandemic can be explained by multiple factors. Delayed presentation of pediatric patients, in general, and higher incidence of NOM during pandemic, are the most important ones. Socioeconomics and delay in time from admission to surgery because of pandemic protocols could be speculated as minor factors [30] . As previously mentioned, the risk of perforation and other complications increases in a linear fashion with duration of disease, especially with pre-hospital delay more than with admitted children. Studies included in the meta-analysis had different findings in regard to delays from the onset of symptoms to admission at the emergency department. Several studies recorded longer prehospital delay in admission of acute appendicitis during the pandemic [9, 31, 35, 37, 38, 41] , while other studies showed no significant difference between pandemic and pre-pandemic delay of presentation [31, 34, 36, 38, 40, 42] . Significant increases in delayed care for different medical emergencies, including pediatric surgical emergencies, during the COVID-19 pandemic period have been noted by the medical community and published in several reports [65] . The effects of the COVID-19 pandemic are recorded in other urgent pediatric surgery conditions such as testicular torsion, in which latest studies show significantly longer time from testicular torsion symptom onset to presentation during the pandemic and a significantly higher proportion of patients reported delaying care [17, 66] . Recent studies show that the outbreak of the COVID-19 pandemic is associated with a delay in presentation of patients with most common medical emergencies such as acute ischemic stroke and delay of diagnosis of colorectal carcinomas, which will lead to a massive downstream impact on healthcare [67, 68] . Delayed presentation can be explained by avoidance of unnecessary hospital visits in the absence of severe symptoms and reduced or delayed access to medical care due to parental fear of children's exposure to As per the findings from recent adult/pediatric studies, the patients developing appendicitis during the pandemic reach healthcare facilities on time (similar to the prepandemic period). Although an identical management algorithm of acute appendicitis was followed during the two time periods, more reliance on non-operative management was observed among the surgeons during the COVID-19 pandemic [69, 70] . Our analysis shows the same result. The main reasons for NOM were the risk of false negative testing and prevention of viral transmission to healthcare workers in the operating room as well as to minimize hospital resource utilization. Additionally, it could be speculated that more patients asked for non-surgical treatment strategies during the pandemic as compared with the cases before the outbreak, with the fear of hospital admission and acquiring the COVID-19 infection from the hospital. Open surgery is suggested as a possible approach because of the shorter operation time and lower risk of COVID-19 transmission [71, 72] . Widespread use of laparoscopic approach and surgeons not being familiar with open surgery could be a reason for higher incidence and more reliance on NOM during pandemic. Fonseca et al. reported a 56% reduction in the number of appendectomies performed in pandemic group in comparison with pre-pandemic, and Percul et al. reported a reduction of 25% in total number of acute appendicitis cases [31, 40] . It could be speculated that during COVID-19 pandemic, patients with mild or non-specific symptoms were not seeking medical care because of the concern about acquiring COVID-19 infection. The number of cases that resolved on their own or are treated with antibiotics prescribed by gatekeepers should also be considered. Confounding variables such as movement restrictions, difference between mild and strict lockdown restrictions, travel restrictions, limited resources, studies being researched mainly in tertiary centers, and other pandemic-induced changes should also be acknowledged [36] . Here additional research is needed and data from outpatient medical care needs to be researched. The results of this study clearly demonstrated a significantly higher number of complicated appendicitis during the COVID-19 pandemic compared with pre-COVID-19 period. Additionally, the study demonstrates an increase in NOM of appendicitis during the pandemic. Both outcomes are direct effect of the COVID-19 pandemic. Management of pediatric appendicitis during this pandemic must be evaluated individually for every hospital and its capacity for SARS-CoV-2 testing, laboratory tests, imaging options, bed, staff, emergency ward capacity and personal protective equipment capacity. Although appendectomy should not be impacted by restrictions on elective procedures several institutions, countries and professional associations recommend performing NOM for appendicitis during pandemic [73] . COVID-19 fundamentally changes the way emergency wards and hospitals function and deliver patient care. The overflow of COVID-19 patients and the effects of the pandemic on health systems, in general, influenced emergency and pediatric specialties wards, which condensed as both elective and emergency care across pediatric specialties decreased and many of these wards were converted to adult wards to accommodate the overflow of adult COVID-19 patients. There were numerous considerations and limitations to consider while delivering patient care, attempting to limit hospital stays while also limiting the number of operations. In conclusion, COVID-19 is a global pandemic, challenging healthcare systems worldwide. During these challenging times, we address the importance of a comprehensive evaluation, physical examination, appropriate and effective treatment in children suspected of having any surgical condition. Balance should be achieved between measures designed to end the pandemic and the appropriate care of pediatric population requiring surgical care. The results of this review must be interpreted within the context of few limitations. First, all except one of the included comparative studies had a moderate risk of bias. Second, the retrospective nature of all studies, except one, is a source of information bias due to variable reporting. Only eight studies reported the proportion of children managed via the NOM. A similar variable reporting was observed in terms of the baseline characteristics of the included studies. The average duration of symptoms and the average hospital stay were non-uniformly reported (both in terms of the averages and the means of dispersion). Third, this meta-analysis involves the pooling of heterogeneous data (I 2 >50% for the primary outcome). Although we excluded the studies where patients were managed during nonidentical months of the pandemic and pre-pandemic periods, the study durations were still not constant across all the included studies. Some other factors that might have contributed to the heterogeneity are the inclusion of the subjects from different geographical locations, variations in socioeconomics in different parts of the world, etc. Finally, these patients were managed by different surgeons in different healthcare facilities. Differences in the management protocols at these centers might have affected the appendectomy and NOM rates. The results of this meta-analysis depict a significantly higher incidence of complicated appendicitis in children during the COVID-19 pandemic than in the pre-COVID-19 period. Additionally, a significantly higher proportion of children were managed via the NOM during the pandemic in comparison to the pre-pandemic period. However, the moderate risk of bias of majority of the included studies prevents us from deriving an appropriate estimate of the overall effect. Further studies with more homogeneous study samples need to be conducted before a definite conclusion is drawn. Author Contributions: Conceptualization, S.A., Z.P. and N.K.; formal analysis, S.A., A.S. and N.K.; writing-original draft preparation, T.Ž., Z.K. and Z.P.; writing-review and editing, S.A. and Z.P. All authors have read and agreed to the published version of the manuscript. Funding: This research received no external funding. Informed Consent Statement: Not applicable. The data presented in this study is available upon request of the respective author. The authors declare no conflict of interest. Web of science-Query 1: ((ALL = (coronavirus)) OR ALL = (SARS-CoV-2)) OR ALL =(COVID-19)) OR ALL = (novel coronavirus) AND Query 2: ALL = (appendicitis). Refine search using keyword "children". SCOPUS-(TITLE-ABS-KEY (appendicitis) AND TITLE-ABS-KEY (covid19 OR coronavirus OR SARS-CoV-2) AND TITLE-ABS-KEY (children OR pediatric). Overview and diagnosis of acute appendicitis in children Validity of appendicitis inflammatory response score in distinguishing perforated from non-perforated appendicitis in children Jurić, I. Prospective validation of Alvarado score and pediatric appendicitis score for the diagnosis of acute appendicitis in children Influence of age on clinical presentation, diagnosis delay and outcome in pre-school children with acute appendicitis Acute appendicitis in children younger than five years of age: Diagnostic challenge for pediatric surgeons Analysis of predictive factors for perforated appendicitis in children Hyponatremia as a predictor of perforated acute appendicitis in pediatric population: A prospective study Hyperbilirubinemia as an indicator of perforated acute appendicitis in pediatric population: A prospective study COVID-19 outbreak: Migration, effects on society, global environment and prevention Questionnaire for assessing social contacts of nurses who worked with coronavirus patients during the first wave of the COVID-19 pandemic Elective surgery cancellations due to the COVID-19 pandemic: Global predictive modelling to inform surgical recovery plans Do surgical emergencies stay at home? Observations from the first United States coronavirus epicenter Reduction in ST-segment elevation cardiac catheterization laboratory activations in the United States during COVID-19 pandemic Potential indirect effects of the COVID-19 pandemic on use of emergency departments for acute life-threatening conditions-United States Reduced rate of hospital admissions for ACS during Covid-19 outbreak in northern Italy Is there an increased incidence of orchiectomy in pediatric patients with acute testicular torsion during COVID-19 pandemic? A retrospective multicenter study Acute appendicitis does not quarantine: Surgical outcomes of laparoscopic appendectomy in COVID-19 times Where did all the appendicitis go? Impact of the COVID-19 pandemic on volume, management, and outcomes of acute appendicitis in a nationwide, multicenter analysis Management of appendicitis during COVID-19 pandemic; short-term outcomes Influence of COVID-19 confinement measures on appendectomies in Germany-a claims data analysis of 9797 patients. Langenbeck's Arch Appendicitis during the COVID-19 lockdown: Results of a multicenter analysis in Germany Rate of application and outcome of non-operative management of acute appendicitis in the setting of COVID-19: Systematic review and meta-analysis How COVID-19 pandemic influences paediatric acute appendicitis cases? Impact of the SARS-CoV-2 pandemic on the course and treatment of appendicitis in the pediatric population Preferred reporting items for systematic reviews and meta-analyses: The PRISMA statement The feasibility of creating a checklist for the assessment of the methodological quality both of randomised and non-randomised studies of health care interventions The measurement of observer agreement for categorical data Cochrane Handbook for Systematic Reviews of Interventions (Version 6.2). 2021. Available online: www.training. cochrane.org/handbook Impact of COVID-19 on the presentation and evolution of acute appendicitis in pediatrics Impact of COVID-19 outbreak on the emergency presentation of acute appendicitis Appendicitis in children in a large italian COVID-19 pandemic area Rate of pediatric appendiceal perforation at a children's hospital during the COVID-19 pandemic compared with the previous year Increased incidence of perforated appendicitis in children during COVID-19 pandemic in a bavarian multi-center study Complicated acute appendicitis during COVID-19 pandemic: The hidden epidemic in children Perforated appendicitis during a pandemic: The downstream effect of COVID-19 in children Delayed presentation and sub-optimal outcomes of pediatric patients with acute appendicitis during the COVID-19 pandemic Impact of the COVID-19 pandemic on appendicitis in COVID-19 negative children Increasing incidence of complicated appendicitis during COVID-19 pandemic Impact of the COVID-19 pandemic on the pediatric population with acute appendicitis: Experience at a general, tertiary care hospital Paediatric appendicitis during the COVID-19 pandemic Statewide impact of the COVID pandemic on pediatric appendicitis in California: A multicenter study Influence of the coronavirus 2 (SARS-Cov-2) pandemic on acute appendicitis Increase in pediatric perforated appendicitis in the New York city metropolitan region at the epicenter of the COVID-19 outbreak The role of a pediatric tertiary care center in avoiding collateral damage for children with acute appendicitis during the COVID-19 outbreak Acute appendicitis in children during the COVID-19 pandemic: Neither delayed diagnosis nor worse outcomes Factors predictive of complicated appendicitis in children Missed appendicitis in a pediatric emergency department Appendiceal perforation in children diagnosed in a pediatric emergency department Clinical features of misdiagnosed appendicitis in children Effect of delay in presentation on rate of perforation in children with appendicitis Association of neighborhood socioeconomic disadvantage with complicated appendicitis in children Insurance-related differences in the presentation of pediatric appendicitis Improving the diagnostic accuracy of appendicitis using a multidisciplinary pathway Comparison of open and laparoscopic appendectomy in children: A 5-year single center experience Incidence and causes of 30-day readmission rate from discharge as an indicator of quality care in pediatric surgery Comparison of new versus reused Harmonic scalpel performance in laparoscopic appendectomy in patients with acute appendicitis-a randomized clinical trial Acute appendicitis and ileal perforation with a toothpick treated by laparoscopy Antibiotic treatment and appendectomy for uncomplicated acute appendicitis in adults and children: A systematic review and meta-analysis Origin, transmission, diagnosis and management of coronavirus disease 2019 (COVID-19) An analysis of changes in emergency department visits after a state declaration during the time of COVID-19 The impact of the COVID-19 pandemic on emergency department visits and patient safety in the United States Delayed access or provision of care in Italy resulting from fear of COVID-19 The impact of COVID-19 on paediatric emergency department visits. A one-year retrospective study A multicenter study of acute testicular torsion in the time of COVID-19 Delayed presentation of acute ischemic strokes during the COVID-19 crisis Impact of COVID-19 on colorectal cancer disparities and the way forward Antibiotics alone as an alternative to appendectomy for uncomplicated acute appendicitis in adults: Changes in treatment modalities related to the COVID-19 health crisis Changes in appendicitis treatment during the COVID-19 pandemic-A systematic review and meta-analysis Managing appendicitis during the COVID-19 pandemic in the UK Risks of viral contamination in healthcare professionals during laparoscopy in the Covid-19 pandemic Management of acute appendicitis during the COVID-19 pandemic: Views of two Brazilian surgical societies. Rev. Colégio Bras