key: cord-0832031-tcn10pho
authors: Moreau, Gregory Brett; Burgess, Stacey L.; Sturek, Jeffrey M.; Donlan, Alexandra N.; Petri, William A.; Mann, Barbara J.
title: Evaluation of K18-hACE2 Mice as a Model of SARS-CoV-2 Infection
date: 2020-07-28
journal: Am J Trop Med Hyg
DOI: 10.4269/ajtmh.20-0762
sha: 73754c7dcefa7cc3007e0253307c6f619bd55304
doc_id: 832031
cord_uid: tcn10pho

Murine models of SARS-CoV-2 infection are critical for elucidating the biological pathways underlying COVID-19. Because human angiotensin-converting enzyme 2 (ACE2) is the receptor for SARS-CoV-2, mice expressing the human ACE2 gene have shown promise as a potential model for COVID-19. Five mice from the transgenic mouse strain K18-hACE2 were intranasally inoculated with SARS-CoV-2 Hong Kong/VM20001061/2020. Mice were followed twice daily for 5 days and scored for weight loss and clinical symptoms. Infected mice did not exhibit any signs of infection until day 4, when no other obvious clinical symptoms other than weight loss were observed. By day 5, all infected mice had lost around 10% of their original body weight but exhibited variable clinical symptoms. All infected mice showed high viral titers in the lungs as well as altered lung histology associated with proteinaceous debris in the alveolar space, interstitial inflammatory cell infiltration, and alveolar septal thickening. Overall, these results show that the K18-hACE2 transgenic background can be used to establish symptomatic SARS-CoV-2 infection and can be a useful mouse model for COVID-19.

An invaluable step in identifying effective vaccines and therapies to combat COVID-19 is the availability of a mouse model of infection. The host receptor for SARS-CoV-2 is the human angiotensin-converting enzyme 2 (hACE2), 1 which was previously identified as the receptor for the SARS-CoV-1 2 that causes SARS, a disease that emerged from China in 2002. 3 The mouse ACE2 ortholog, which has significant amino acid sequence variation in the viral receptor binding domain, cannot serve as an efficient receptor for either SARS-CoV-2 or CoV-1. 4 A transgenic mouse model to study SARS-CoV-1 infection was developed that expresses the hACE2 gene under the control of the human cytokeratin 18 promoter. 5 Infection of these mice with SARS-CoV-1 results in a rapidly lethal infection. 5 Four other hACE2-expressing mouse lines have been created to date and tested for the ability to support SARS-CoV-2 infection. Two lines express the hACE2 gene under the control of the mouse ACE2 promotor 6, 7 ; one was made using the CRISPR/Cas9 technology. 7 The third strain uses the lung ciliated epithelial cell hepatocyte nuclear factor-3/ forkhead homologue 4 (HFH4) promoter. 8, 9 An additional approach was to transfect wild-type mice with an adenovirus carrying the hACE2 gene. 10 Overall, with the exception of the HFH4 mice, in which there was some lethality, infection of these three mouse strains with SARS-CoV-2 results in mild clinical symptoms and no lethality. Here, we report the infection of K18-hACE2 with SARS-CoV-2. Although this infection resembled that of other strains, we observed variable clinical presentation, with some mice exhibiting more severe symptoms than reported using other models. Overall, this work supports the usefulness of K18-hACE2 transgenic mice as a model for human COVID-19 infections.

To investigate the potential of this transgenic mouse strain as a model for COVID-19 infection, five K18-hACE2 mice were intranasally inoculated with 8 × 10 4 Median Tissue Culture Infectious Dose (TCID50) of SARS-CoV-2, and five mice were mock-infected with sterile Dulbecco's Modified Eagle's Medium (DMEM). Mice were followed twice daily for 5 days and scored for clinical symptoms (weight loss, eye closure, appearance of fur [piloerection] and posture, and respiration). The mock-infected mice did not exhibit any clinical symptoms or experience any weight loss throughout the experiment. Infected mice did not exhibit any measurable clinical symptoms through day 3. On day 4, no other clinical symptoms other than weight loss were observed. On day 5, all the infected mice had lost around 10% of their original weight ( Figure 1A ) and exhibited variability in other clinical signs of infection, with clinical scores ranging from 3 to 9 (maximal score 14) ( Figure 1B ). Although two of the infected K18-hACE2 mice showed only mild symptoms at day 5 (weight loss and reduced activity), two mice exhibited piloerection. The most severe mouse had increased respiration, lethargy, and slight eye closure and met our criteria for euthanasia. Because the study was ended on day 5, it is unclear whether the remaining four mice would have recovered if the study was carried past day 5.

Although the clinical severity was variable between infected K18-hACE2 mice, our results suggest that these mice present with more symptomatic disease than other hACE2 mouse models of SARS-CoV-2 infection. In the mouse model expressing hACE2 under the mouse ACE2 promoter, infected mice did not exhibit any clinical symptoms other than maximal weight loss on day 3 postinfection, and those mice recovered. 7 Only mild ruffling of fur and up to 8% weight loss on day 5 were observed in the other model using the mouse ACE2 promoter, and once again, all mice recovered. 6 In mice transfected with an adenovirus carrying the hACE2 gene, mice exhibited about a 10% weight loss on day 4 postinfection but no lethality. 10 In contrast to these models, in which mice exhibited mild symptoms and recovered, only 60% of the mice survived past day 5 in the mouse strain expressing hACE2 under the lung ciliated epithelial cell HFH4 promoter. 9 Although this model had higher lethality, weight loss was only about 5% and these mice had no respiratory symptoms. The authors hypothesize that mortality may be due to neuroinvasion because virus was detected in the brain. In K18-hACE2 mice infected with SARS-CoV-1, the course of infection is clearly different; the infection is uniformly fatal, beginning on day 4 postinfection, and mice were symptomatic with labored breathing and lethargy. 5 Although the number of mice used in this study was small and we were not able to measure survival, our data support a difference in the disease progression between these two viruses.

All mice were euthanized on day 5, and tissue was collected for dissection and enumeration of viral loads. No significant differences in histology of the spleen, small intestine, or liver were observed between infected and mock-infected mice, and these tissues were normal in size and appearance. Dissection of the lungs of infected mice revealed a mottled or marbled appearance that was not observed in mock-infected mice (data not shown). Lung sections were analyzed after staining with hematoxylin and eosin and scored based on tissue pathology. 11 SARS-CoV-2-infected mice exhibited significantly higher histopathology scores than mock-infected mice ( Figure 2 ). The major histopathology findings in infected mice were proteinaceous debris in the alveolar space, neutrophils in the interstitial space, and alveolar septal thickening ( Figure 2 ); these observations were consistent with other hACE2 mouse models, which also detected signs of lung injury including interstitial pneumonia, inflammatory cell infiltrates, and alveolar septal thickening. 6, 7 Consistent with the observed infiltrating neutrophils, granulocytes and inflammatory monocytes were also elevated in the bronchoalveolar lavage (BAL) fluid from the infected mice ( Figure 3 ).

Other hACE2 mouse models of COVID-19 infection have observed high viral titers in the lungs with limited viral load in organs such as the liver and spleen during intranasal infection. 6,7 Although we did not investigate viral load in the liver or spleen, these organs appeared normal by histology, suggesting that there was limited viral titer in these tissues. Virus was detected in the lungs of all infected mice, with titers generally in the range of 1 × 10 5 plaque forming units (PFU)/mL (Table 1 ). Viral titers in the lungs appeared somewhat associated with disease severity: mouse 1390, which had the highest lung titer, had the highest clinical score, histopathology score, and percent weight loss at day 5 (Table 1 ) and the highest numbers of neutrophils, monocytes, and eosinophils in the BAL (Figure 3 ). In addition, mouse 1413, which had the lowest titer, had the lowest clinical score, second lowest percent weight loss at day 5 (Table 1) , and lowest number of eosinophils and monocytes in the BAL (Figure 3 ). Of note, mouse 1413 did not have the lowest histopathology score (Table 1) . Although there were trends toward higher viral titers in the lungs being associated with higher clinical and histopathology scores, these trends were not significant, and viral titer was not a strong predictor of percent weight loss. The power of this analysis is limited by the small sample size, but these results suggest that factors in addition to viral load, such as inflammatory responses, are driving the severity of disease. This would also potentially explain the sudden onset of clinical symptoms at 5 days post-inoculation.

In this report, we have described the course of SARS-CoV-2 infection in K18-hACE2 transgenic mice. Our findings are consistent with other studies using hACE2 mice, which observed successful infection with SARS-CoV-2 and a milder disease severity compared with SARS-CoV-1. 6, 7 The onset of symptoms was abrupt, manifesting on day 5. Mice exhibited a similar degree of weight loss but a varying degree of symptoms and clinical/histopathological scores. The number of mice used in this study was too small to determine whether this was a result of experimental variability or natural variability in outcomes. The variance in clinical and histopathological scores may be partially explained by viral titer, but there are likely other factors, such as the host immune response, that contribute to the variance observed. The observation of more severe disease in a subset of the K18-hACE2 mice is distinct from other hACE2-expressing COVID-19 models, which typically observed only mild clinical symptoms. 6, 7 This could be due to experimental differences such as strain differences or the challenge dose (Table 2 ). To date, little is known about the possibility of virulence differences among isolates. Hong Kong/VM20001061/2020 and strain 2019n-CoV/USA_WA1/ 2020 are closely related and have been classified as Type IB. 12 The receptor-binding domains of these strains are 100% identical (data not shown). The phylogeny of HB-01 and Wuhan/AMMS01/2020 has not been reported. The challenge dose used in each experiment is similar; our experiment used the lowest amount of inoculum. The resident microbiota in each mouse strain could also impact outcomes of infection. The other difference between these strains is in the level of hACE2 receptor expression or tissue distribution. Nonetheless, K18-hACE2 transgenic mice may be a particularly useful , and respiratory rate (0-2). All mouse work was approved by the university's institutional animal care and use committee, and all procedures were performed in the university's certified animal biosafety level three laboratory.

Histology. Tissues were fixed in formaldehyde. Slides were scanned at ×20 magnification. Histopathological scoring for lung tissues was performed according to the guidelines of the American Thoracic Society. 11 A two-tailed Student's t test was used to determined statistical significance.

Viral titers. The left lobe of the lung was homogenized in 1 mL serum-free DMEM with a disposable tissue grinder. Plaque assays were performed as described. 13 In brief, Vero C1008, Clone E6 (ATCC CRL-1586) cells grown in DMEM (GIBCO 11995-040) with fetal bovine serum (FBS) were seeded into at a concentration of 2 × 10 5 cells/well the night before the assay. Serial dilutions were added to the wells. The plate was incubated at 37°C, 5% CO 2 for 2 hours, shaking the plates every 15 minutes. After 2 hours, the media was replaced with a liquid overlay of DMEM, 2.5% FBS containing 1.2% Avicel PH-101 (Sigma Aldrich, St. Louis, MO) and incubated at 37°C, 5% CO 2 . After 3 days, the overlay was removed, wells were fixed with 10% formaldehyde, and stained with 0.1% crystal violet to visualize plaques. Plaques were counted, and PFUs were calculated according to the following equation: Average # of plaques/dilution factor × volume diluted virus added to the well. 8, 9 Not specified Not specified ∼ 5% Weight loss, no clinical symptoms, but only 60% survived Adenovirus transfection 10 10 5 focus-forming units Strain 2019n-CoV/USA_WA1/2020 10% Maximum weight loss, all recovered hACE2 = human angiotensin-converting enzyme 2. 

This work was supported by NIH grants R01 AI124214 to W. A. P., R01 AI146257 to S. L. B., and 5T32AI007496-23 to A. N. D., and the University of Virginia's Global Infectious Diseases Institute. J. M. S. is a an iTHRIV scholar, a program supported in part by the National Center For Advancing Translational Sciences of the NIH under award numbers UL1TR003015 and KL2TR003016

Disclaimer: The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH

Division of Infectious Diseases and International Health

Receptor recognition by the novel coronavirus from Wuhan: an analysis based on decade-long structural studies of SARS coronavirus

Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus

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Acknowledgments: We would like to gratefully acknowledge the advice and assistance of Angelina Angelucci and Young Hahn at the University of Virginia as well as Caitlin Woodson and Kylene Kehn-Hall at George Mason University. Publication charges for this article were waived due to the ongoing pandemic of COVID-19.

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