key: cord-0830933-9ir6ud76 authors: Du, Xue; Gao, Yang; Kang, Kai; Chong, Yang; Zhang, Mei-Ling; Yang, Wei; Wang, Chang-Song; Meng, Xiang-Lin; Fei, Dong-Sheng; Dai, Qing-Qing; Zhao, Ming-Yan title: COVID-19 patient with an incubation period of 27 d: A case report date: 2021-07-26 journal: World J Clin Cases DOI: 10.12998/wjcc.v9.i21.5955 sha: f0dece8b9a96a11b657203874df1cd40b3000319 doc_id: 830933 cord_uid: 9ir6ud76 BACKGROUND: As a highly contagious disease, coronavirus disease 2019 (COVID-19) is wreaking havoc around the world due to continuous spread among close contacts mainly via droplets, aerosols, contaminated hands or surfaces. Therefore, centralized isolation of close contacts and suspected patients is an important measure to prevent the transmission of COVID-19. At present, the quarantine duration in most countries is 14 d due to the fact that the incubation period of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is usually identified as 1-14 d with median estimate of 4-7.5 d. Since COVID-19 patients in the incubation period are also contagious, cases with an incubation period of more than 14 d need to be evaluated. CASE SUMMARY: A 70-year-old male patient was admitted to the Department of Respiratory Medicine of The First Affiliated Hospital of Harbin Medical University on April 5 due to a cough with sputum and shortness of breath. On April 10, the patient was transferred to the Fever Clinic for further treatment due to close contact to one confirmed COVID-19 patient in the same room. During the period from April 10 to May 6, nucleic acid and antibodies to SARS-CoV-2 were tested 7 and 4 times, respectively, all of which were negative. On May 7, the patient developed fever with a maximum temperature of 39℃, and his respiratory difficulties had deteriorated. The results of nucleic acid and antibody detection of SARS-CoV-2 were positive. On May 8, the nucleic acid and antibody detection of SARS-CoV-2 by Heilongjiang Provincial Center for Disease Control were also positive, and the patient was diagnosed with COVID-19 and reported to the Chinese Center for Disease Control and Prevention. CONCLUSION: This case highlights the importance of the SARS-CoV-2 incubation period. Further epidemiological investigations and clinical observations are urgently needed to identify the optimal incubation period of SARS-CoV-2 and formulate rational and evidence-based quarantine policies for COVID-19 accordingly. open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See 19) , which is caused by severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infection, has been officially identified as a Class B infectious disease mainly involving the respiratory system. In China, the prevention and control measures for COVID-19 are required to be in accordance with Class A infectious disease[1]. SARS-CoV-2 is a novel coronavirus[2], and all individuals are susceptible to this infection. At present, no effective targeted intervention has been proposed beyond supportive treatment [3, 4] . The successful experience of antiepidemic measures in China suggest that a series of multi-faceted public health interventions can effectively control the outbreak of COVID- 19[5] , of which centralized isolation of close contacts and suspected patients is one of the most important measures to prevent the transmission of COVID-19. The quarantine duration in most countries is 14 d based on the predicted longest incubation period of SARS-CoV-2. It is true that cases with an incubation period of more than 14 d are rare in the clinic[6]. However, it is still of great concern to avoid the rapid spread of the epidemic from cases with an incubation period of more than 14 d, since the infected person has strong infectivity in the incubation period [7, 8] . We present a COVID-19 patient with an incubation period of 27 d confirmed in the Fever Clinic of The First Affiliated Hospital of Harbin Medical University. July 26, 2021 Volume 9 Issue 21 A 70-year-old male patient was admitted to the Department of Respiratory Medicine of The First Affiliated Hospital of Harbin Medical University on April 5 due to a cough with sputum and shortness of breath. Cough with sputum and shortness of breath appeared 2 mo ago, and the cough and dyspnea were then further aggravated. The patient had a medical history of hypertension and vitiligo. The patient had no personal or family history. The patient's vital signs were stable. Rales could be heard on auscultation of both lungs. Laboratory tests revealed the following results on admission: White blood cell count of 9.06 × 10 9 /L, neutrophil (NEUT) count of 7.34 × 10 9 /L, NEUT% of 80.90%, lymphocyte (LYMPH) count of 0.47 × 10 9 /L and percentage of lymphocytes (LYM%) of 5.20%. During the period from April 10 to May 7, blood cell analyses were reexamined (Table 1 ), in which LYMPH and LYM% significantly decreased. The initial lung computed tomography (CT) indicated pneumonia and multiple nodules in the lungs, bilateral pleural effusion and pericardial effusion. On May 7, reexamination of the lung CT showed patchy shadows and interstitial changes. On May 11, lung CT imaging showed that the lung lesions had rapidly deteriorated ( Figure 1 ). On May 7, the results of nucleic acid and antibody detection of SARS-CoV-2 were positive. On May 8, the nucleic acid and antibody detection of SARS-CoV-2 by Heilongjiang Provincial Center for Disease Control were also positive, and the patient was diagnosed with COVID-19 and reported to the Chinese Center for Disease Control and Prevention. In the initial stage of disease, the patient occasionally had shortness of breath but did not need oxygen therapy. On April 10, the patient was transferred to the Fever Clinic for further treatment due to close contact to one confirmed COVID-19 patient in the same room. During the period from April 10 to May 6, lung CT was reviewed on April 26 and 30 (Figure 1 ), and nucleic acid and antibody of SARS-CoV-2 were tested 7 and 4 times, respectively, all of which were negative (Table 1) . Surprisingly, on May 7, the patient developed fever with a maximum temperature of 39℃, and his respiratory difficulties had deteriorated. Continuous oxygen therapy was then provided. Due to continuous deterioration of the patient's condition, a high-flow nasal cannula and invasive mechanical ventilation were given to the patient on May 7 and 11 to enhance respiratory support. In addition, comprehensive treatment measures also included antibiotic agents, analgesic and sedative drug injections, vasopressor support and immunotherapy. July 26, 2021 Volume 9 Issue 21 Unfortunately, despite timely comprehensive treatment measures were taken, the patient's immune function and condition did not improve, and he eventually died. In April 2020, a cluster of SARS-CoV-2 infections occurred in two tertiary general hospitals in Harbin city, resulting in 66 newly confirmed patients including this case and 21 asymptomatic patients. Due to close contact to a confirmed COVID-19 patient, our patient had received relevant treatment in the Fever Clinic of our hospital for 27 d. As our hospital is the treatment center for COVID-19 in Heilongjiang Province, the ward layout, regulations, work arrangements, operation specifications and disinfection procedures in the Fever Clinic of our hospital were all in line with national regulations and standards; thus, the possibility of cross-infection in the Fever Clinic was extremely low. Therefore, by staying in the same room with a confirmed COVID-19 patient from April 5 th to 10 th , there was a chance of contracting SARS-CoV-2. Currently, the diagnosis of COVID-19 mainly depends on detection of SARS-CoV-2 nucleic acid on oropharyngeal and/or nasopharyngeal swabs by real-time polymerase chain reaction (RT-PCR)[9,10], although there is still a possibility of false-positive and false-negative results [11] [12] [13] . Therefore, lung CT has been proposed as an auxiliary method for the rapid diagnosis of COVID-19 due to its low rate of missed diagnosis [14] . In addition to a CT scan, abnormal findings on fluorodeoxyglucose-positron emission tomography/CT scans may confirm or exclude the presence of SARS-CoV-2 infection [15] . Bilateral and peripheral ground-glass opacities and consolidation are the most common features of COVID-19 on lung CT imaging [16] [17] [18] , which were not found in the three lung CT examinations before our patient was diagnosed with COVID-19. In addition, the patient's seven nucleic acid tests and four antibody tests for SARS-CoV-2 were all negative during the quarantine period. Following the diagnosis of COVID-19, CT imaging revealed rapid deterioration of the lungs with the typical imaging characteristics of COVID-19 over a short period of time compared with the previous CT scans [19] . Based on the above history, it was concluded that the patient had acquired SARS-CoV-2 infection due to sharing the same room with a confirmed COVID-19 patient from April 5 th to 10 th , he then developed worse symptoms on May 7 th and was confirmed to have COVID-19 on May 8 th . Thus, the incubation period was 27 d, which is very rare in clinical practice. The prerequisite for determining the optimal quarantine duration is a good understanding of the incubation period. The incubation period usually refers to the time between infection and the onset of corresponding symptoms and signs or when the disease is confirmed [20] , which determines the adjustment in quarantine policies. The mainstream view is that the incubation period of SARS-CoV-2 is usually identified as 1-14 d with a median estimate of 4-7.5 d [6, [21] [22] [23] , and thus the current clinical practice of 14-d quarantine duration in most countries seems reasonable. However, there is a different opinion that a small proportion of COVID-19 patients have an incubation period of more than 14 d based on the estimated incubation distribution[6, 20], which will be an enormous figure considering the surge in COVID-19 patients and its rising trend. Age may be one of the explanations for the longer incubation period of COVID- 19[24] , while the other mechanisms are still unclear. Definitive close contact, as shown in this case report, and some extreme cases should be considered to extend the quarantine duration during the outbreak stage of the epidemic [25, 26] . When the epidemic recurred in Heilongjiang Province in April 2020, the quarantine duration was extended and the SARS-CoV-2 nucleic acid test was popularized for entry personnel, referred to as "14 + 7 + 14". Fourteen days of centralized isolation and 2 nucleic acid tests, and 7 d of centralized isolation and one nucleic acid test should be carried out at the entry point and the local region, respectively, and then 14 d of home isolation and observation should be carried out and incorporated into the grid management of the local community [27] . Prolonged quarantine duration can effectively prevent SARS-CoV-2 from spreading during the incubation period. Obviously, an extension of quarantine duration will make the already overwhelmed medical system even more overstretched, and increase the burden on society and individuals during the COVID-19 epidemic. Further epidemiological investigations and clinical observations are urgently needed to identify the optimal incubation period of SARS-CoV-2 and formulate rational and evidence-based quarantine policies for COVID-19 accordingly. As the quarantine duration for COVID-19 in most countries is currently 14 d, cases with an incubation period of more than 14 d in the clinic may trigger rapid spread of the epidemic, which requires us to be highly vigilant. Further studies are needed to determine the proportion of COVID-19 patients with an incubation period of more than 14 d, and weigh the costs of extending quarantine duration and the potential risks and consequences of the spread of the epidemic during the incubation period. These findings will have important implications for optimal prevention and control of COVID-19. National Health Commission of the People's Republic of China. 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We pay tribute to the medical staff who lost their lives in the national fight against the COVID-19 epidemic.