key: cord-0826931-db6g5r9r authors: Drosos, Alexandros A.; Pelechas, Eleftherios; Voulgari, Paraskevi V. title: Seronegative Erosive Arthritis Following SARS-CoV-2 Infection date: 2021-11-16 journal: Rheumatol Ther DOI: 10.1007/s40744-021-00395-9 sha: dc2ed00aab6de28aaf7c7855b5705640a4fc0a41 doc_id: 826931 cord_uid: db6g5r9r Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) affecting mostly the respiratory system, but several other organs and systems can be involved. Extrapulmonary manifestations and autoimmune phenomena following SARS-CoV-2 infection are frequent events occurring during the first 2 weeks or in later stages of the disease course. These can be expressed as an isolated discovery of autoantibodies, mostly antinuclear or antiphospholipid antibodies, through to full-blown autoimmune organ-specific and systemic diseases. Joint pain is a frequent complain in most patients, but to our knowledge, frank arthritis has not been reported so far. A 46-year-old woman developed symmetrical polyarthritis 2 months after SARS-CoV-2 infection. Laboratory tests showed high acute phase reactants, while the immunological profile was negative. Hand and wrists X-rays revealed soft tissue swelling as well as bone erosions at the ulnar base of the third and fourth metacarpophalangeal joint of the right hand and carpal bones. The patient responded well to small doses of prednisone and methotrexate and after 4 months she had a sustained clinical and laboratory improvement. This is the first report making an association between SARS-CoV-2 infection and erosive polyarthritis. Physicians dealing with patients infected from SARS-CoV-2 should be aware for the possible development of musculoskeletal disorders, among them symmetrical polyarthritis. Thus, a close follow-up and monitoring is mandatory. Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a matter of concern worldwide and a challenge for physicians dealing with patients with autoimmune rheumatic diseases (ARD) [1] . Although the inflammation caused by SARS-CoV-2 infection affects mainly the respiratory system, some patients may develop extrapulmonary manifestations (EPM). These are the result of a hyperinflammatory response of the innate and adaptive immunity, caused by an excessive reaction to the SARS-CoV-2 [2, 3] . This exaggerated reaction is characterized by the production and release of a plethora of pro-inflammatory cytokines like interleukin (IL)-1b, IL-6, IL-18, tumor necrosis factor alpha (TNFa), and impaired interferon (IFN) type-I response [2] [3] [4] . Immune-mediated EPM are frequently recognized in patients with COVID-19 and are associated with various autoimmune phenomena [5, 6] . The clinical features range from the detection of an autoantibody, mainly antinuclear antibodies (ANA) or antiphospholipid antibodies (APLs), to a mild and benign disease, but there are cases that lead to full-blown organ-specific and systemic autoimmune diseases like thyroiditis, myositis, myocarditis, vasculitis, peripheral neuropathy, antiphospholipid syndrome (APS), and many others [7, 8] . Arthralgias and joint pain have been reported in about 40% of patients contracting COVID-19. However, frank arthritis with evidence of inflammation upon physical examination of the involved joints, to our knowledge, has not been reported so far. To this end, we report a 46-year-old woman who developed symmetrical polyarthritis affecting the small joints of the hands, 2 months after SARS-CoV-2 infection. Laboratory evaluation revealed high acute phase reactants without specific autoantibodies, while imaging modalities showed erosive changes affecting the third and fourth metacarpophalangeal (MCP) joints of the right hand and carpal bones. The relevant literature was searched and discussed. A written informed consent has been obtained and signed by the patient. All procedures were in accordance with ethical standards and the Helsinki Declaration of 1964 and its later amendments. Ethics committee approval by our institution is not required if manuscripts do not show details of the presented patients. A 46-year-old woman presented on May 4, 2021 with pain and swelling affecting the small joints of the hands bilaterally, lasting for 6 weeks. She received paracetamol and non-steroidal antiinflammatory drugs [(NSAIDs), diclophenac 75 mg bid], without significant improvement. She was a single, non-smoker with negative past medical and family history. However, on February 1, 2021 she had been diagnosed with SARS-CoV-2 infection, based on low grade fever, sore throat, myalgias, arthralgias, and a positive polymerase chain reaction (PCR) for COVID-19. She remained isolated at home, receiving occasionally paracetamol, and 3 weeks later she was free of symptoms. A new test for SARS-CoV-2 infection was negative. However, 1 month later she complained for arthralgias, morning stiffness, and swelling of the small joints of the hands. She repeated a new SARS-CoV-2 test which was negative. Clinical examination revealed swelling and tenderness affecting the MCPs and proximal interphalangeal (PIP) joints bilaterally (Fig. 1 ). She denied photosensitivity, skin rashes, psoriasis, oral ulcers, Raynaud's phenomenon, uveitis, urethritis, and diarrhea. Laboratory tests showed high C-reactive protein (CRP) [60 mg/dl (normal values \ 6)], high erythrocyte sedimentation rate [(ESR) 82 mm/h], and high IgG antibodies against SARS-CoV-2, while a new PCR test was negative. The rest of the laboratory tests including ANA, rheumatoid factor (RF), and anti-citrullinated protein antibodies (ACPA) were all negative. Chest X-rays were negative, while hand X-rays showed soft tissue swelling and erosions affecting the ulnar base of the 3rd and 4th MCP of the right hand and carpal bones, as well as joint space narrowing of the 2nd and 3rd MCPs and radiocarpal joint bilaterally (Fig. 2a) . The aforementioned findings were better visualized with the use of musculoskeletal ultrasonography, which detected an erosive lesion involving the base of the 4th MCP of the right hand measuring 8 mm of diameter (Fig. 2b) . On the basis of the American College of Rheumatology (ACR) classification criteria, 1987 and 2010, she was classified as having seronegative rheumatoid arthritis (RA) [9, 10] . She was treated with methotrexate (MTX), 15 mg/week and folic acid supplements, plus prednisone 10 mg/day. Two months later she had a substantial clinical improvement with normal acute phase reactants; thus, prednisone was tapered. After 4 months the patient had a complete clinical and laboratory improvement receiving MTX 15 mg/day and prednisone 2.5 mg/day. swelling around the PIP joints bilaterally, and an erosive change is evident on the proximal ulnar side of the proximal phalanx of the 4th digit of the right hand (arrow). Note also joint space narrowing of the 2nd and 3rd MCPs and of the radiocarpal joint bilaterally. MCP metacarpophalangeal joints, PIP proximal interphalangeal joints. b Musculoskeletal ultrasound in B-mode. An erosive lesion is evident on the ulnar base of the 4th digit of the right hand (white asterisk) Note also that another erosion is forming more distally DISCUSSION COVID-19, caused by SARS-CoV-2 infection, can lead to severe inflammation and has also been suggested to induce EPM and autoimmune phenomena [7, 8] . Several studies have reported the presence of autoantibodies in patients with COVID-19, mostly ANAs, APLs, and anti-b2glycoprotein I antibodies [11] [12] [13] [14] . ACPAs and RF in low titers have also been described, but frank arthritis with structural damage has not been reported so far. In this report we describe, for the first time, the onset of seronegative erosive RA in a patient after COVID-19. Autoimmune phenomena related to COVID-19 were initially described in severe hospitalized patients, but have been also reported in patients with mild COVID-19 and even in asymptomatic patients. The severity of the autoimmune manifestations of COVID-19 varies, ranging from a benign and self-limiting disease like psoriasis to systemic lupus erythematosus (SLE), or APS and vasculitis [7, 8] . Some autoimmune phenomena in relation to COVID-19 tend to occur during the first 2 weeks, whereas others tend to manifest in a late, post-infectious stage, and also in asymptomatic patients. Little is known about the pathogenesis of these manifestations, since autoantibodies are absent in many cases, as in our patient. One hypothesis has reported the presence of autoantibodies against IFN type I, or inborn errors in type I IFN immunity [15, 16] . Another hypothesis implies that SARS-CoV-2 infection can disturb immunological tolerance by exposure of antigen epitopes that elicit cross-reactive antibodies. There are several reports indicating antigenic mimicry between viral and human proteins. One published example of molecular mimicry is the immune response of Epstein-Barr virus (EBV) in patients with SLE [17, 18] . On the other hand, the etiology of RA is multifactorial. Genetic factors, among them the human leucocyte antigen (HLA) DRB1 shared epitope (SE), hormonal factors, and environmental exposure such as smoking, or infectious agents, may predispose to RA development. The SE hypothesis implies that susceptibility alleles could bind efficiently to arthritogenic peptides (self-antigen or viral peptide), leading to activation of CD4 ? T cells and production of antibodies, like RF and ACPAs, through B cell activation [19] . Several viruses have been postulated as possible etiologic factors in RA, among them EBV, parvovirus B-19 and many others. For example, EBV is a polyclonal activator of B cells and increases the production of RF. Another hypothesis implies that the EBV gp110 protein is one of the many xenoproteins found in SE. Such proteins might trigger responses through the molecule mimicry hypothesis, leading the immune response directed against a similar endogenous protein. Indeed, Gammazza et al. compared viral proteins with human molecular chaperones and postulated that chaperones could participate in molecular mimicry phenomena after SARS-CoV-2 infection [20] . Several other studies suggested molecular mimicry as a possible mechanism underlying the development of autoimmune phenomena in SARS-CoV-2 infection [21] [22] [23] . However, the possibility of other types of seronegative arthritides should be excluded. Reactive arthritis (ReA) is one of them. Usually, ReA is manifested as a mono-or oligoarticular disease affecting mostly the large joints of the body, following a gastrointestinal or genitourinary infection [24] . In the discussed case, our patient presented with symmetrical polyarthritis affecting the small joints of the hands and had no history of urethritis or gastrointestinal infection. Another possibility is the coincidence of seronegative RA with COVID-19 disease, or the presence of RA and its flaring after SARS-CoV-2 infection [14] . This possibility is difficult to exclude; however, our patient had no history of arthralgias, or signs and symptoms of arthritis before SARS-CoV-2 infection. Finally, the presence of erosive disease in this case and its chronological relationship with COVID-19 disease is a matter of discussion, since structural damage in RA is manifested late during the disease course. On the other hand, erosive changes can also be seen in patients with early RA and this finding can be assumed as an unfavorable prognostic factor for RA outcome [25] . Treatment-wise, these autoimmune phenomena can be combated depending on the clinical features and their severity. In cases of systemic manifestations, small to high doses of steroids with or without immunosuppressive drugs are required. In mild cases, close follow-up and monitoring may be sufficient. In our patient, small doses of prednisone along with MTX were sufficient to suppress the inflammatory process. We searched Medline and Scopus sources using the following keywords and found no other cases published so far: SARS-CoV-2 and erosive symmetrical polyarthritis or/and COVID-19 and symmetrical erosive disease. In conclusion, we described a patient with seronegative erosive RA following SARS-CoV-2 infection. Early recognition of the specific types of autoimmune phenomena and diseases after COVID-19 is an imperative for physicians and will allow them to have an accurate diagnosis and treatment. Thus, close follow-up and monitoring is mandatory. Funding. No funding or sponsorship was received for this study or publication of this article. Authorship. All named authors meet the International Committee of Medical Journal Editors (ICMJE) criteria for authorship for this article, take responsibility for the integrity of the work as a whole, and have given their approval for this version to be published. Author Contributions. Alexandros A. Drosos: concept and design, critical review. Eleftherios Pelechas: drafting the manuscript, bibliographic evaluation. Paraskevi V. Voulgari: critical review, drafting. Disclosures. Alexandros A. Drosos, Eleftherios Pelechas, and Paraskevi V. Voulgari have nothing to disclose. Compliance with Ethics Guidelines. All procedures were in accordance with ethical standards and the Helsinki Declaration of 1964 and its later amendments. Ethics committee approval by our institution is not required if manuscripts do not show details of the presented patients. Data Availability. Data sharing is not applicable to this article as no datasets were generated or analyzed during the current study. Open Access. 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