key: cord-0826055-l8nevb3x
authors: Sookaromdee, Pathum; Wiwanitkit, Viroj
title: Identification of SARS-CoV-2 in post-mortem nasopharyngeal swabs
date: 2021-05-11
journal: Forensic Sci Int
DOI: 10.1016/j.forsciint.2021.110829
sha: 7f974a91e8a205770af779e1755e457d4fd20afe
doc_id: 826055
cord_uid: l8nevb3x

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Dear Editor, we would like to share the ideas on "Forensic evaluation of two nucleic acid extraction systems and validation of a RT-qPCR protocol for identification of SARS-CoV-2 in post-mortem nasopharyngeal swabs [1] ." Barrio et al. concluded that "Both the automated and the manual RNA extraction procedures showed good efficiency, but the automated virus extraction by bio-robot produced more reproducible results than the manual extraction [1] ." Generally, a robotic RNA purification protocol usually has a better threshold for detection of RNA of virus [2] . However, there are still many considerations on automated extraction. In general, the automatic extraction has to be based on commercial kit. The commercial RNA extraction kits may be insufficient when there is a large demand during outbreak [3] .

Additionally, comparing to manual approach, there are problems of in-valid rate and problem of negative agreement of automatic approach [4] . Barrio et al. mentioned for cross reactivity study but there is no specific study on cross reactivity to other viruses [1] . In a complete assessment of an automated extraction approach, it is necessary to perform a specific assessment on many respiratory viruses. In a recent report, Li et al. reported a similar evaluation with a complete cross reactivity analysis was performed in 20 other respiratory viruses [5] . Additionally, the reproducibility is also associated with stability. The effect of transport media on stability of virus should be mentioned [6] . Using of a lysis buffer supplemented with nucleic acid stabilization mix can also help extend stability [7] . To conclude on the stability property of new automated extraction should recognize the effect of transportation and specimen preparation.

Forensic evaluation of two nucleic acid extraction systems and validation of a RT-qPCR protocol for identification of SARS-CoV-2 in post-mortem nasopharyngeal swabs

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