key: cord-0823450-8c8o3j03
authors: Sakai, Takashi; Herrmann, Nadine; Maintz, Laura; Nümm, Tim Joachim; Welchowski, Thomas; Claus, Ralf A.; Gräler, Markus H.; Bieber, Thomas
title: Altered Serum Phospholipids in Atopic Dermatitis and Association with Clinical Status
date: 2021-12-22
journal: JID Innov
DOI: 10.1016/j.xjidi.2021.100092
sha: 8b931b36c27d098f51f4ca25e98037bd7942a548
doc_id: 823450
cord_uid: 8c8o3j03

Circulating phospholipids have been considered as biomarkers and therapeutic targets in multiple disorders. Atopic dermatitis (AD) is the most common inflammatory skin disease. Although there are numerous studies having addressed stratum corneum lipids in the context of epidermal barrier, little is known about the circulating lipids in patients with AD. In this study, we explored the changes of serum phospholipids in AD using liquid chromatography coupled to tandem mass spectrometry and sought serum lipids’ contribution to clinical status. Several serum levels of phospholipids were altered in the AD group (n = 179) compared with that in healthy controls (n = 47) and patients without AD with atopic comorbidities (n = 22); lipids exhibiting the apparent changes included increased sphingosine, multiple variants of phosphatidylcholine, and decreased ceramide (16:0) in patients with AD. Moreover, serum levels of sphingosine correlated with the severity of AD, and sphingosine and ceramide(16:0) were also detected as the risk-increasing effect and risk-reduction effect of AD, respectively. In summary, alterations in the serum concentration of phospholipids are seen in patients with AD. Although more detailed investigations will be needed to evaluate the significance of the changes in circulating lipids in AD, these findings can provide, to our knowledge, previously unreported insight into AD’s pathogenesis and therapeutic strategies.

Atopic dermatitis (AD) is the most common inflammatory skin disease with a strong impact on the QOL of patients and their relatives and a significant socioeconomic burden. Epidermal barrier dysfunction and T helper 2-mediated inflammation-inducing pruritus are hallmarks of AD. In addition, AD is often associated with comorbidities such as allergic rhinitis, bronchial asthma, or food allergy and subsequent physical and psychological pain (Weidinger et al., 2018) . Although there are numerous studies having addressed stratum corneum lipids in the context of epidermal barrier (Borodzicz et al., 2016; Park et al., 2019) , little is known about the circulating lipids in patients with AD. Metabolomics is an approach for studying metabolic changes connected to clinical significance as well as for finding biomarkers, and it can provide new insight into diseases' pathogenesis. It is well-known that circulating levels of ceramide (Cer) and sphingolipids have emerged as predictive and diagnostic biomarkers of cardiometabolic complications, including coronary artery disease, diabetes, and heart failure (Choi et al., 2021) and several neurodegenerative disorders, including Alzheimer's disease and Parkinson's disease (Alessenko and Albi, 2020) . Those sphingolipids in cardiovascular and neurodegenerative disorders have also been considered therapeutic targets (Alessenko and Albi, 2020; Choi et al., 2021) . Furthermore, disturbances in circulating sphingolipids levels and their association with the phenotype have been observed in inflammatory and allergic disorders, such as systemic lupus erythematosus (Watson et al., 2012) , psoriasis (Kowalski et al., 2013; My sliwiec et al., 2017) , or bronchial asthma (Kim et al., 2020; Kowal et al., 2019) . Recently, we reported that serum concentrations of sphingosine (Sph)-1-phosphate (S1P), which is a bioactive lipid mediator, in patients with AD are higher than those in healthy individuals and patients without AD with atopic comorbidities (Sakai et al., 2021) . S1P receptors are therapeutic targets in multiple disorders (Cartier and Hla, 2019) , and the clinical trials with the compounds targeting S1P receptors in patients with AD are underway (NCT04162769, ACTRN12617000763347). Although other serum phospholipid levels have been evaluated in animal models with allergic dermatitis-eczema horses had significantly lower concentrations of phosphatidylcholine (PC) and sphingomyelin than their healthy controls (Hallamaa and Batchu, 2016 )-they have not been examined in patients with AD. We hypothesized that serum phospholipids in patients with AD are altered, which may be associated with clinical findings of AD. In this study, we explored the changes of serum phospholipids in AD with metabolomics and sought serum lipids' contribution to clinical status.

This study consisted of three groups: healthy controls (n ¼ 47), those with AD (n ¼ 179), and those with non-AD with atopic comorbidities (i.e., non-AD with allergic rhinitis, bronchial asthma, and/or food allergy, n ¼ 22). We recruited these participants as a part of the Christine Kü hne-Center for Allergy Research and Education (Davos, Switzerland) program (Bieber et al., 2020) , who were the same as those recruited in our previous study (Sakai et al., 2021) . We evaluated the serum concentrations of 40 lipids in all participants (Table 1) . Patients with AD showed increased serum levels of Sph and multiple variants of sphingomyelin and PC and decreased serum levels of Cer(16:0), with statistical significance, compared with healthy controls and those with non-AD with atopic comorbidities. Serum levels of lysoplatelet-activating factor in the AD group were not changed compared with those in the healthy controls and non-AD with atopic comorbidities group. Regarding serum concentrations of lysophosphatidylcholine (LPC), one variant of LPC (LPC[18:2]) was decreased in AD group compared with that in healthy controls, whereas one variant of LPC (LPC[16:0]) was increased in AD group compared with that in non-AD with atopic comorbidities group. The serum levels of three variants of lysophosphatidylethanolamine (LPE) were decreased in the AD group compared with that in healthy controls, whereas one variant of LPE was increased in the AD group compared with that in the non-AD with atopic comorbidities group. About comparing between healthy controls and non-AD with comorbidities group, serum levels of four variants of LPE, lyso-platelet-activating factor, and Cer(16:0) were decreased in non-AD with atopic comorbidities group compared with that in healthy controls (Table 1 and Figure 1 ).

Next, we evaluated the correlation between these serum lipids concentrations and clinical and laboratory data in AD ( Figure 2 and Tables 2 and 3). Body mass index showed no correlation with all evaluated serum phospholipid levels, with statistical significance. Although correlation coefficients were low, serum levels of Sph positively correlated with body surface area (r ¼ 0.281, P ¼ 0.032) and serum levels of thymus and activation-regulated chemokine (r ¼ 0.277, P ¼ 0.032). Other serum phospholipid levels showed no significant correlation with any clinical and laboratory data ( Figure 2 and Tables 2 and 3).

To evaluate which phospholipids have a strong association when predicting AD or non-AD (healthy controls and non-AD with atopic comorbidities group), we performed penalized Lasso regression. A total of 17 of 40 variables were selected that are associated with the risk of AD. The strongest risk-increasing effect was Sph, and the strongest riskreduction effects were Cer(16:0) and LPE(18:0) (Table 4 and Figure 3 ). We also evaluated these serum lipids levels in the AD subgroups stratified by AD's severity (AD [eczema area and severity index [EASI] < 16] vs. AD [EASI ! 16]) or atopic comorbidities (pure AD vs. AD with atopic comorbidities). Serum Sph levels were elevated in moderate to severe AD compared with those in mild to moderate AD. There were no differences in serum Cer(16:0) and LPE(18:0) levels between those AD subgroups ( Figure 4 ).

Several serum levels of phospholipids were altered in the AD group compared with those in the other groups; lipids exhibiting the apparent changes included increased Sph and multiple variants of PC and decreased Cer(16:0) in patients with AD. Moreover, serum levels of Sph, correlating with body surface area and thymus and activation-regulated chemokine, were elevated in moderate to severe AD, such as circulating S1P in AD (Sakai et al., 2021) . Sph and Cer(16:0) were also detected as the risk-increasing effect and riskreduction effect of AD, respectively. These findings suggest that altered serum levels of phospholipids might be involved in AD's pathogenesis or biomarkers reflecting systemic inflammation owing to dermatitis. The important roles of sphingolipids metabolites, especially in immune systems (Cinque et al., 2003) and as biomarkers in multiple disorders, have been well-established. Serum levels of Sph are associated with the symptomatic phenotypes of patients with COVID-19 (Janneh et al., 2021) and are fluctuated in patients with hepatitis B virus or hepatitis C virus by virologic conditions (Grammatikos et al., 2016; Mü cke et al., 2018) . In patients with rheumatoid arthritis, serum Sph and Cer levels are increased (Miltenberger-Miltenyi et al., 2020) , and the PC/LPC ratios of plasma represent a measure of inflammation (Fuchs et al., 2005) . PC has been reported as a factor related to the effect of omalizumab in AD (Hotze et al., 2014) , suggesting the association between increased serum Sph and PC (and S1P) levels and inflammation in AD.

In contrast, the significance of decreased serum Cer(16:0) levels in AD is obscure. Among the lipids whose serum levels were altered in cases of AD, the strongest change was seen for Cer(16:0) as well as for some variants of PC, suggesting that serum Cer(16:0) levels might serve as a biomarker indicating the severity of disease phenotype. However, there were no significant differences in serum Cer(16:0) levels between AD subgroups stratified by the severity of AD or between pure AD and AD with atopic comorbidities, and no significant correlation between serum Cer(16:0) levels and all clinical and laboratory data was observed. Contrary to We analyzed 40 lipids concentrations in serum from healthy individuals, individuals with AD, and individuals with non-AD with atopic comorbidities (allergic rhinitis, bronchial asthma, and/or food allergy). This figure is a graphical summary of altered lipids in the sera of the AD group compared with those of the healthy controls or non-AD with atopic comorbidities group, summarizing Table 1 . AD, atopic dermatitis; Cer, ceramide; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; PAF, plateletactivating factor; PC, phosphatidylcholine; SM, sphingomyelin; Sph, sphingosine. systemic levels, Cer(16:0) increases in the skin of patients with AD (Berdyshev et al., 2018) . The observed decreases in circulating Cer(16:0) levels in AD are inconsistent with reports indicating that this marker is altered (increased) in multiple other diseases such as neurodegenerative diseases (Pujol-Lereis, 2019), acute ischemic stroke (Gui et al., 2020) , and chronic kidney disease (Mantovani et al., 2021) . Serum levels of Cer(16:0) in patients with uncontrolled asthma are higher than those in patients with controlled asthma (Kim et al., 2020) . In tissues such as the liver and adipose tissue, long-chain Cer(16:0) is deleterious that its signaling is considered therapeutic targets in some disorders, including obesity and type 2 diabetes (Choi et al., 2021; Raichur et al., 2019) . Because there were significant differences in the serum levels of Sph, Cer(16:0), and S1P (Sakai et al., 2021) between patients with AD and both healthy controls and non-AD with atopic comorbidities group, we speculate that patients with AD may reflect systemic changes in sphingolipid syntheses or that the presence of large areas of eczematous skin in which the production of these lipids is altered may affect the syntheses at systemic levels; further analyses will be required to evaluate this hypothesis. Altered metabolism of sphingolipids can affect the immune system (Alaamery et al., 2021; Cartier and Hla, 2019) , which might be a therapeutic target or associated with severe AD's pathogenesis.

Because this study is observational, we cannot judge whether altered serum lipids are the pathogenic factors of AD or the results owing to systemic inflammation. Further studies may provide new insight into AD's pathogenesis and therapeutic strategies. Indeed, circulating sphingolipids can work as predictive biomarkers, such as drug efficacy and disease prognosis in other disorders, as well as diagnostic biomarkers (Hsu et al., 2019; Xie et al., 2021) . In AD, such prospective studies with metabolomics will be desired in the future. Moreover, we evaluated serum phospholipids in patients with AD at only one time point and did not evaluate skin barrier function and FLG mutations in this study; therefore, multiple timepoint analyses of serum phospholipids and the relationship between serum phospholipids and skin barrier function are of great interest among the future perspectives within Christine Kü hne-Center for Allergy Research and Education. The experiments using animal models with altered serum sphingolipids are helpful to evaluate the pathogenesis of altered serum lipids in AD. It would be interesting to know whether such models (e.g., mice with decreased serum Cer [16:0] levels) can develop severe dermatitis or not by several stimuli. Our survey also revealed the differences in serum levels of LPE and lyso-platelet-activating factor between healthy controls and non-AD with atopic comorbidities group. In this study, although the number of patients with non-AD with atopic comorbidities was not high enough, these serum lipids might evolve to new biomarkers in allergic disorders other than AD. The association between AD and obesity has been established (Ali et al., 2018) , and serum sphingolipids levels are changed depending on body mass index in patients with psoriasis (Kozłowska et al., 2019) . Therefore, the evaluation of serum phospholipids in patients with AD with obesity, compared with those in patients with AD with normal body weight or healthy individuals, would be informative to investigate the mechanisms between AD and obesity. The results presented in this study provide several ideas for future approaches in this arising field.

There are some limitations to this study. First, this observational study does not provide a mechanism behind the study, and our laboratory analyses were limited to 40 serum lipids. Other Cers than Cer(16:0) were not as readily detected and therefore were excluded from the analysis. Second, allergic rhinitis, bronchial asthma, and food allergy were diagnosed solely on the basis of the participants' self-reported diagnoses, and the number of patients without AD with atopic comorbidities was small compared with the numbers of participants in the other groups.

In summary, alterations in the serum concentration of lipids were seen in patients with AD, including increases in Sph and PC and decreases in Cer(16:0); the changes in Sph were associated with AD severity. More detailed investigations will be needed to evaluate the significance of the changes in circulating lipids in the pathogenesis of AD, especially in the context of the development of lipid-related treatments for this major chronic dermatologic condition.

The participants were recruited in the Christine Kü hne-Center for Allergy Research and Education program. They were part of an ADfocused registry program with a comprehensive collection of phenotypic and epidemiologic information as well as biobanking (Bieber et al., 2020) . This study enrolled a total of 248 participants: Abbreviations: AD, atopic dermatitis; BMI, body mass index; BSA, body surface area; Cer, ceramide; EASI, eczema area and severity index; Ig, immunoglobulin; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; PAF, platelet-activating factor; PC, phosphatidylcholine; SCORAD, severity scoring of atopic dermatitis; SM, sphingomyelin; Sph, sphingosine; TARC, thymus and activation regulated chemokine.

We analyzed the correlation between serum lipids concentrations and clinical and laboratory data in patients with AD (n ¼ 179). The r values were calculated by Spearman's correlation test, and the P-values were adjusted using Benjamini-Hochberg procedure. Table 2 shows the r values supporting 179 patients with AD, including 30 patients with AD only (pure AD) and 149 patients with AD with atopic comorbidities (i.e., allergic rhinitis, bronchial asthma, and/or food allergy); 22 patients without AD with atopic comorbidities; and 47 healthy controls. These study groups were the same as those used in our previous study (Sakai et al., 2021) . The study was approved by the ethics committee of the University of Bonn (Bonn, Germany) and was performed in accordance with the principles of the Declaration of Helsinki. Written informed consent was obtained from each participant in the study. The diagnosis of AD was performed according to the criteria of Hanifin and Rajka (Akdis et al., 2006) . Clinical scoring of AD (i.e., severity scoring of atopic dermatitis, EASI, and body surface area [%]) was performed by experienced dermatologists in the Dermatology Department at the University Hospital Bonn (Bonn, Germany) (Wollenberg et al., 2018a (Wollenberg et al., , 2018b . We defined AD with EASI !16 as moderate-to-severe AD and AD with EASI <16 as mild-to-moderate AD, which is currently the cut-off for the indication for systemic therapy (Beck et al., 2014) . Information on pruritus and sleeplessness was extracted from the subjective severity scoring of atopic dermatitis. Diagnosis of allergic rhinitis, bronchial asthma, or food allergy was conducted on the basis of the information provided by the subjects on a self-reported diagnosis. Blood samples were collected Abbreviations: AD, atopic dermatitis; BMI, body mass index; BSA, body surface area; Cer, ceramide; EASI, eczema area and severity index; Ig, immunoglobulin; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; PAF, platelet-activating factor; PC, phosphatidylcholine; SCORAD, severity scoring of atopic dermatitis; SM, sphingomyelin; Sph, sphingosine; TARC, thymus and activation regulated chemokine.

We analyzed the correlation between serum lipids concentrations and clinical and laboratory data in patients with AD (n ¼ 179). The r values were calculated by Spearman's correlation test, and the P-values were adjusted using Benjamini-Hochberg procedure. Table 3 shows the P-values supporting Figure 2 .

T Sakai et al.

from participants and immediately processed to serum using a standardized procedure; the resulting sera were stored frozen at e80 o C until assessment. The patients' demographics are shown in the previous literature (Sakai et al., 2021) . Briefly, participants were limited to adults (aged 20e59 years), and patients with any systemic or chronic disorder requiring hospital visits other than AD and atopic comorbidities (allergic rhinitis, bronchial asthma, or food allergy) were excluded. There were no differences among the three study groups with regard to age, body mass index, or sex. There was no apparent bias in the AD group with regard to age, body mass index, EASI, body surface area, total IgE levels, or AD duration. Moreover, in the AD group, onsets of AD of the patients were 1.0 (0.17e8.25) years (median [Q1eQ3]), and 82.6% of the patients developed AD in childhood and adolescence (less than 18 years). None of the patients with AD had received systemic treatment for AD, including systemic corticosteroids and biologics, within 30 days before sample collection. In contrast, 82.4% of the patients with AD had received the treatment with topical corticosteroids within the preceding year. Regarding ethnicity, 11 participants were Asian, and all other participants were German. Serum levels of thymus and activationregulated chemokine were measured by MSD U-PLEX Multiplex Assays (Meso Scale Diagnostics, Rockville, MD) according to the manufacturer's instructions. This biomarker was measured in all patients with AD.

Serum lipid analyses were performed in all participants using liquid chromatography coupled to tandem mass spectrometry. We evaluated 40 lipids in the serum of all participants. These lipids belong to the most prevalent group of phospholipids and were chosen because these lipids were readily detected and could be well-quantified. Cer(16:0) is the most abundant Cer that was assessed. As mentioned in the limitations of the main text, other Cers than Cer(16:0) were not as readily detected and therefore were excluded from the analysis. Protein precipitation was performed in 96-well plates from Sarstedt (Nuremberg, Germany; K-series) from 20. We sought the serum phospholipids being associated with the risk of AD with L1 penalized logistic regression between AD and non-AD, which includes healthy controls and those with non-AD with atopic comorbidities. We analyzed the serum concentrations of (a) Sph, (b) Cer(16:0), and (c) LPE(18:0) in the study groups: healthy individuals, patients with AD, and patients without AD with atopic comorbidities (allergic rhinitis, bronchial asthma and/or food allergy) or in the AD subgroups stratified by EASI or in the AD subgroups with or without atopic comorbidities. Significances were calculated with the two-tailed Mann-Whitney U test (middle and right panels) or with the Kruskal-Wallis test followed by pairwise comparisons with Mann-Whitney U test. The P-values for multiple comparisons were adjusted using Benjamini-Hochberg procedure (left panels). Healthy controls (n ¼ 47), patients with AD (n ¼ 179), patients with non-AD with atopic comorbidities (n ¼ 22); AD (EASI < 16) (n ¼ 130), AD (EASI ! 16) (n ¼ 49); pure AD (n ¼ 30), AD with atopic comorbidities (n ¼ 149). AD, atopic dermatitis; Cer, ceramide; EASI, eczema area and severity index; LPE, lysophosphatidylethanolamine; Sph, sphingosine.

mobile phase changed to 10% B, and the flow rate decreased linearly from 1.0 ml/min at 10 minutes to 0.5 ml/min at 10.5 minutes and remained constant until the end of the program at 11.3 minutes. Detection took place between 2 and 10 minutes. The injection volume per sample was 30.0 ml; samples were cooled at 4 C. The

HPLC system was coupled to the API 2000 triple-quadrupole mass spectrometer (Sciex, Foster City, CA) equipped either with an ESI or an APCI source, both operating in a positive mode under the following source parameters: source temperature of 450 C, curtain gas of 40, low collision gas, ion spray voltage of 5,500 (ESI), nebulizer current of 4 (APCI), ion source gas1 of 30 (ESI) or 60 (APCI), and ion source gas2 of 60 (ESI) or 30 (APCI). Target ions Q1 > Q3, assignment to an internal standard, and mode of analysis are given in Table 5 . The analytical results were quantified with Analyst 1.6.2 (AB Abbreviations: APCI, atmospheric pressure chemical ionization; Cer, ceramide; ESI, electrospray ionization; SM, sphingomyeline; Sph, sphingosine; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; PC, phosphatidylcholine. For Cer, SM, and lyso-compounds, the number of carbon atoms is given and also the number of unsaturated bonds; for SM, the sphingoid backbone is given in brackets; and for PC, the total number of carbon atoms as well as of unsaturated bonds are given.

Sciex, Forster City, CA) on the basis of internal standard samples and an external standard curve.

Data are presented using the median with 25th and 75th percentiles (Q1eQ3) or the box plots. We applied the two-tailed Mann-Whitney U test when comparing two groups and the Kruskal-Wallis test followed by pairwise comparisons with Mann-Whitney U test when comparing three groups. Spearman's correlation was used to compare continuous variables. The P-values for multiple comparisons were adjusted using Benjamini-Hochberg procedure. A P < 0.05 was considered statistically significant. In addition, we sought the serum phospholipids being associated with the risk of AD using L1 penalized logistic regression between AD and non-AD, which includes healthy controls and non-AD with atopic comorbidities. The regularization parameter lambda was chosen by 10-fold crossvalidation on the basis of the binomial deviance criterion. All analyses were performed using SPSS Statistics, version 25 (IBM, Armonk, NY); Prism, version 9.2.0 (GraphPad Software, San Diego, CA); and R, version 4.1.0 (R Core Team (2021) (R: A language and environment for statistical computing, Foundation for Statistical Computing, Vienna, Austria; https://www.R-project.org/) with packages glmnet_4.1-2, car_3.0-10, and openxlsx_4.2.4.

Datasets related to this article can be found at https://data.mendeley. com/datasets/tgjfsdb4t4/1, hosted at Mendeley Data.

Diagnosis and treatment of atopic dermatitis in children and adults: European Academy of Allergology and Clinical Immunology/ American Academy of Allergy

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This work was supported by the Christine Kü hne-Center for Allergy Research and Education. The funding source did not participate in study design, collection and interpretation of data, in the writing of the report and in the decision to submit the article for publication.