key: cord-0821831-eilvoe7v
authors: Huang, Jing; Li, Jie; Zou, Zhenwei; Kandathil, Asha; Liu, Jun; Qiu, Shaohong; Oz, Orhan K.
title: Clinical Characteristics of 3 Patients Infected withCOVID-19: Age, Interleukin 6 (IL-6), Lymphopenia, and Variations in Chest Computed Tomography (CT)
date: 2020-10-14
journal: Am J Case Rep
DOI: 10.12659/ajcr.924905
sha: 425bff03dd8089838b73eb83cd4f09baae18b90d
doc_id: 821831
cord_uid: eilvoe7v

Case series Patients: Female, 69-year-old • Female, 38-year-old • Male, 37-year-old Final Diagnosis: COVID-19 Symptoms: Fever Medication: — Clinical Procedure: — Specialty: Infectious Diseases OBJECTIVE: Unknown ethiology BACKGROUND: COVID-19 has been identified as the cause of the large outbreak of pneumonia in patients in Wuhan with shared history of exposure to the Huanan seafood market; however, there is more to learn about this disease. Some experts report that the virus may have reduced toxicity during transmission, but others say that toxicity does not change during transmission. CASE REPORTS: In this case series, we report clinical and imaging characteristics of 3 patients (A, B, and C) infected with COVID-19. In an exposure-tracking epidemiological investigation, we found that it is possible that Patient A transmitted the infection to her treating physician, Patient B. Patient B then likely transmitted the infection to her family member, Patient C. From the chest CT studies and clinical characteristics, we postulate that the virulence did not decrease during human-to-human transmission. In previous studies, patients with the virus infection had changes in chest CT; however, we found that during the early stages of this disease, some patients (Patient C) may have normal chest CT scans and laboratory studies. Most importantly, we found that IL-6 levels were highest and lymphocyte count was lowest in those with more severe infection. CONCLUSIONS: In this case series, we report the exposure relationship of the 3 patients and found that chest CT scans may not have any changes at the beginning of this disease. Lymphopenia and elevated levels of IL-6 can be found after infection.

Recently, COVID-19, a corona virus pneumonia outbreak which began in Wuhan Hubei province in China, has rapidly become a global health issue all over the world [1] [2] [3] . COVID-19 is estimated to have resulted in 4 483 864 cases in 185 countries with 303 825 deaths as of May 15, 2020 . Many countries chose to lock down or order "stay at home" to prevent virus rapid transmission and protect people [4] [5] [6] . Some researchers report that the virus may have reduced toxicity during transmission or even suddenly disappear when the temperature rises; however, other researchers insist that COVID-19 toxicity does not change during transmission [7] [8] [9] .

From the current experience with COVID-19, death has more often been seen among the older people (age >60 years). Those with pre-existing conditions or diseases are more susceptible to the new virus and their condition can suddenly and rapidly progress from an asymptomatic infection, including slight fever, cough, or diarrhea, to severe pneumonia, respiratory failure, and multiple organ failure, sometimes leading to death [2, 10] . The progression is closely related to an "inflammatory storm" involving a number of different cytokines. Laboratory analysis shows these patients tend to have lymphopenia, thrombocytopenia, and increased or even normal C-reactive protein (CRP), lactate dehydrogenase, and interleukin levels [11] . The main CT findings of COVID-19 are ground-glass opacities (GGOs) and patchy consolidations, which are also the main indicators of disease initiation and progression [12] . However, not all patients follow the same pattern, and there are patients who are symptomatic and have positive nucleic acid test results but have normal CT scan or laboratory tests, which may be negative or low during treatment. In the present case series, we report the clinical characteristics, radiological findings, treatment, and outcome of 3 patients with COVID-19, illustrating the range of severity and outcomes.

A 69-year-old woman presented with headache, vomiting, low-grade fever, and a maximum body temperature of 37.5°C on January 9, 2020. She did not appear to have other diseases and never smoked. Her symptoms did not improve much after taking over-the-counter cold medicine and amoxicillin. She had dizziness, vomiting, cough, chest tightness, muscle soreness, and other discomforts, and her body temperature gradually increased to 38.5°C. Her initial chest CT was normal (not shown). She was unsuccessfully treated with moxifloxacin at a local hospital in Wuhan on January 12, 2020 and then came to our outpatient facility on January 14, 2020. A chest CT showed multiple, peripheral ground-glass opacities in both lungs suggestive of viral pneumonia ( Figure 1A ). Tests for influenza A and influenza B viruses were negative. Laboratory tests revealed CRP: 70.61 mg/L and lymphocyte: 0.78×10 9 /L ( Table 1) . Cytokine detection in blood revealed IL-6: 15.86 pg/ml ( Table 2 ). The nucleic acid test for COVID-19 was positive on January 15, 2020. A throat swab was used to collect samples to extract COVID-19 RNA from patients suspected of having COVID-19 infection. We used a respiratory sample RNA isolation kit (Zhongzhi, Wuhan, China) and this suspension was used for real-time reverse transcription polymerase chain reaction (RT-PCR) analysis by using a COVID-19 nucleic acid detection kit according to the manufacturer's protocol (Shanghai Bio-Reproductive Medicine Technology Co., Ltd.). These diagnostic criteria are based on the recommendations of the National Institutes for Disease Control and Prevention (http://ivdc.chinacdc.cn/kyjz/202001/t20200121_211337.html). The Ct value is defined as a medium load from 37 to less than 40 and needs to be confirmed by retesting. After treatment with moxifloxacin, vancomycin, and oseltamivir on January 16, 2020, blood oxygen decreased to SPO2=92%. Subsequently, after January 16, 2020, she was given linezolid, voriconazole, and moxifloxacin, oseltamivir, and gamma globulin, with no improvement. She died on January 22, 2020, 13 days after the onset of fever.

A 38-year-old woman began to have chest tightness on January 19, 2020. She started to have fever on January 22, 2020, with body aches, dry cough, and a body temperature of up to 37.4°C. Her temperature normalized after physical cooling. Prior to this, she was in good health and does not smoke. The nucleic acid test for COVID-19 was positive on January 23 using the same methods as described above, and the diagnosis of 2019-nCoV infection was confirmed. On January 24, 2020, she was admitted to the hospital for treatment. Routine blood tests showed lymphocytes: 0.84×10 9 /L ( Table 1) . Cytokine detection in blood revealed an IL-6=2.94pg/ml ( Table 2) . Tests for influenza A and influenza B viruses were negative. A chest CT on day 5 showed a few small left lower-lobe opacities ( Figure 1B ). On January 24, 2020, she was started on moxifloxacin, oseltamivir, and gamma globulin and was placed in isolation. At present, the patient has recovered and is in good stable general health.

A 37-year-old man developed fatigue on January 27, 2020, and then developed fever up to 37.6˚C. He was in good health before and never smoked. On January 28, 2020, the nucleic acid test for COVID-19 was positive but the chest CT scan was normal ( Figure 2 ). Routine blood tests showed lymphocyte= 0.97×10 9 /L ( Table 1) . Cytokine detection in blood revealed IL-6=8.54 pg/ml (Table 2) . Later, he was admitted to the hospital for isolation and observation. After treatment with moxifloxacin, oseltamivir, and gamma globulin, his body temperature was normal on January 30, 2020, but a chest CT on day 5 showed multiple small ground-glass opacities in both lungs ( Figure 1C ). At present, he has recovered and is in good stable general health.

Comparing the 3 cases, we found older age and higher inflammatory status with increasing cytokines such as IL-6 or CRP are associated with poor outcome. Lymphopenia is a common finding. While the association with inflammation and the elevated IL-6 levels have been reported, our patients became ill early in the pandemic and were presenting these findings. These cases show that patients can have slight symptoms and normal chest CT in the early phases of the disease. Non-severe patients may have normal or small opacities on their chest CT. The presence of peripheral ground-glass opacities with bilateral distribution, multi-lobar and posterior involvement, and subsegmental vessel enlargement appear to be common characteristics of those with severe COVID-19 pneumonia [13, 14] .

Patient A became symptomatic on January 9, 2020 and was treated by Patient B, who is an infectious disease physician, beginning January 14, 2020. Patient B became symptomatic with fever 8 days later on January 22, 2020 and did not have contact with any other patients with COVID-19 infection. Patient B performed a tracheotomy on patient A on January 17, 2020. Therefore, we speculate that patient B was very likely to have been infected by patient A. Patient C, who is patient B's husband, became symptomatic on the 4 th day after patient B was confirmed to be infected. In the week before he became infected, he did not have contact with other people outside his home, so we speculate that patient C was infected by patient B.

From the perspective of the clinical symptoms of these 3 patients, we found that the clinical symptoms of patient A were the most severe. The symptoms of Patient B and Patient C were almost the same. The disease progression of Patient A was the fastest, with only 13 days from fever to death. The patient's blood oxygen decreased on the 9 th day, and there was no significant improvement with administration of large amounts of oxygen. The rapid disease progression in Patient A may be related to the older age (69 years old). For patient A, after having cold-like symptoms, she took cold medicine and amoxicillin for 1 week from January 9 to January 15. On January 16, 2020 she was given moxifloxacin, vancomycin, and oseltamivir treatment, but the blood oxygen still decreased. Therefore, after January 16, 2020, the doctor modified the treatment by using linezolid, voriconazole, and moxifloxacin, oseltamivir, and gamma globulin. However, none of these treatments improved the patients' condition. Patient A took several antibacterial drugs because she had an infection and had fever and increased CRP (70.61 mg/L). All medications were used strictly according to instructions; therefore, the risk of medication adverse effects seems low. The death of Patient A may also be closely related with high inflammation status (IL-6: 15.86 pg/ml, CRP 70.61mg/L). In addition, patient A may have missed the best treatment window due to delayed viral nucleic acid detection and normal chest CT when she first presented, both of which are easily ignored by doctors in clinical practice. Additionally, for treatment, ECMO is a very limited resource in this hospital. Therefore, patient A did not have a chance to receive it before she died. Patient B did not show a decrease in blood oxygen, the fever was controlled, and fatigue improved. Patient C was likely infected by Patient B, but had slightly worse symptoms than Patient B. Moreover, the time from infection to the time of presentation to physicians for both patient A and B infected was estimated in the range from 1 to 14 days (most likely 3-10 days). Given the short time between infections, it is likely the virulence of the virus did not change during transmission, although gene sequencing of the virus may be a better option to explain the viral pathogenicity and transmissibility. Age may have had some protective effect for patient B. Some studies have shown that men tend to have higher risk for infection and more severe symptoms compared with women [15] [16] [17] [18] [19] [20] . However, related studies have been hampered by a large heterogeneity in testing and reporting of the data. Based on clinical practice, it seems men have higher death rates compared to women. However, the data is confusing when taking the health condition, comorbidities, and hormone levels into consideration. The proportion of positive tests is almost equal in men and women, which indicates they still have a similar risk of infection with COVID-19 [8, 21] .

None of these 3 patients received hydroxychloroquine and/or azithromycin treatment since that the drug scheme was not available at the time they were admitted to clinics in January 2020 [22] [23] [24] .

Chest CT findings were variable in our case series, but in general the findings were worse with increasing severity of infection. Figure 1 shows chest CT images on day 5 of illness for all 3 patients. The CT findings for patient A are the most extensive (Figure1A), with a large area of inflammatory changes in both lungs. The patient died from respiratory failure 8 days later. Chest CT in Patient B shows only a few ground-glass changes in the left lower lung (Figure1B) . The initial chest CT scan in Patient C showed no abnormalities in the early stages of the disease (Figure 2 ). After 1 week, there were some lower-lobe opacities ( Figure 1C ). Figure 3 shows the disease course in the 3 patients. During the follow-up of patients B and C, chest CT scan results for Patient B (March 12 th ) and Patient C (February 27 th ) returned to normal, with no previous CT changes ( Figure 4 ). The laboratory results show that the WBC count and neutrophil count were normal in all 3 patients; however, the lymphocytes were reduced ( Table 1 ). The cytokine test results showed that IL-6 was significantly increased (Table 2) . Cytokines reflect the degree of inflammation of the lungs and indicate that Patient A had severe inflammation. The degree of increase in IL-6 can be used as a laboratory test indicator for the degree of infection and inflammatory changes associated with COVID-19 infection. From chest CT screen and laboratory findings, we believe the severity of COVID-19 infection can be inferred at the initial presentation to the healthcare system.

Our analysis of these 3 cases did not show that the virus lost virulence during transmission. Further gene sequencing of the virus is urgently needed to definitively correlate virulence with severity of infection [25, 26] . In addition, patients infected with the virus may not have changes on chest CT in the early stages of disease, which is easily ignored by doctors in clinical practice. Thus, early testing of CVID-19 nucleic acid and other cytokine levels would be better options to find infected people. Among cytokine tests, IL-6 seems to be sensitive detector since it can change during the disease initiation. Moreover, IL-6 can reflect the degree of infection and inflammatory changes associated with COVID-19. Indeed, a review of clinical trials related to COVID-19 at clinical trials.gov shows 2 studies are targeting IL-6 signaling through targeting the IL-6 receptor with Sarilumab. In summary, older age, a high inflammatory status (cytokine level increased), lymphopenia, and bilateral multilobar opacities on chest CT are closely associated with higher severity and mortality from COVID-19 infection in our series. Other comorbidities, especially lung and cardiovascular diseases and diabetes, increase the risk of death from COVID-19 infection, although it appears our 3 patients did not have these.

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