key: cord-0820937-1c7h6aix authors: Colaneri, Marta; Sacchi, Paolo; Zuccaro, Valentina; Biscarini, Simona; Sachs, Michele; Roda, Silvia; Pieri, Teresa Chiara; Valsecchi, Pietro; Piralla, Antonio; Seminari, Elena; Di Matteo, Angela; Novati, Stefano; Maiocchi, Laura; Pagnucco, Layla; Tirani, Marcello; Baldanti, Fausto; Mojoli, Francesco; Perlini, Stefano; Bruno, Raffaele title: Clinical characteristics of coronavirus disease (COVID-19) early findings from a teaching hospital in Pavia, North Italy, 21 to 28 February 2020 date: 2020-04-23 journal: Euro Surveill DOI: 10.2807/1560-7917.es.2020.25.16.2000460 sha: 71213187bdaa23e605e2f82368bc0478509e039e doc_id: 820937 cord_uid: 1c7h6aix We describe clinical characteristics, treatments and outcomes of 44 Caucasian patients with coronavirus disease (COVID-19) at a single hospital in Pavia, Italy, from 21–28 February 2020, at the beginning of the outbreak in Europe. Seventeen patients developed severe disease, two died. After a median of 6 days, 14 patients were discharged from hospital. Predictors of lower odds of discharge were age > 65 years, antiviral treatment and for severe disease, lactate dehydrogenase > 300 mg/dL. We describe clinical characteristics, treatments and outcomes of 44 Caucasian patients with coronavirus disease (COVID-19) at a single hospital in Pavia, Italy, from 21-28 February 2020, at the beginning of the outbreak in Europe. Seventeen patients developed severe disease, two died. After a median of 6 days, 14 patients were discharged from hospital. Predictors of lower odds of discharge were age > 65 years, antiviral treatment and for severe disease, lactate dehydrogenase > 300 mg/dL. Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is an emerging virus recently detected and associated with a severe respiratory disease, named coronavirus disease (COVID- 19) , which has been declared a pandemic disease by the World Health Organization (WHO) on 11 March 2020 [1] . As at 20 April 2020, 1,149,071 confirmed cases of COVID 19 were reported in Europe, of which 181,228 were in Italy [2] . To date, while there are numerous studies describing clinical characteristics of COVID-19 patients from China [3] [4] [5] [6] , there are only few published from European countries. Here we report early findings on clinical presentation, treatment and clinical outcomes of patients with COVID-19 in a large teaching hospital in Pavia, Lombardy region, northern Italy, as well as preliminary analyses of predictors of discharge status and of developing severe disease. We extracted data from medical records of all consecutive patients admitted with a diagnosis of COVID-19, between 21 and 28 February 2020 and followed-up until 4 March 2020. We collected demographic data (sex and age), clinical data (symptoms on admission, comorbidities and chest X-ray results), laboratory tests and treatment data (use of antivirals, antibiotic drugs and oxygen support). Diagnosis of COVID-19 was confirmed by positive real-time reverse transcriptase PCR from nasal swabs, which were analysed by the molecular virology unit of our hospital according to the WHO guidelines and protocol by Corman et al. [7] . Severe disease was defined as requirement for highflow oxygen support. Low (cannula and simple masks) and high-flow (Venturi and reservoir masks) oxygen support were provided when hypoxia defined as peripheral oxygen saturation < 90% was detected. Univariate logistic analysis was employed to identify variables associated with hospital discharge and development of severe disease. The multivariable model was built to determine the association between the variables which threshold values were <0.10 at univariate analysis. In this final model, variables with a p value < 0.05 were considered statistically significant. From 21 February to 28 February 2020, 44 confirmed cases of COVID-19 were observed. The majority were males (28 males, 16 females). Median age was 67.5 years (range: 10-94 years, interquartile range (IQR): 29.1) and 19 patients were under 65 years old. Clinical signs on admission were fever (n = 40), cough (n = 15 ), dyspnoea (n = 10), diarrhoea (n = 3), weakness (n = 2); confusion, respiratory failure, constipation, chest pain and muscle pain were each reported by one patient (Table 1) . Remarkably, 16 patients had no underlying comorbidities. The most common comorbidities were hypertension (n = 15), heart disease (n = 11), diabetes mellitus (n = 7), history of cancer (n = 6; five solid carcinomas, one B-cell lymphoma), lung diseases (n = 2) and chronic hepatitis C (n = 2). Only one patient had chronic kidney disease. We observed no cases with known immunodeficiency, either congenital or acquired. Chest X-ray revealed the presence of 31 cases of interstitial pneumonia (n = 31). Table 1 shows laboratory findings on admission. Lymphocytopenia was present in 39 patients, thrombocytopenia was observed in 19, and leukopenia in 22. CD4+ T-cell count was < 250 cells/μL in 14 patients. LDH levels were elevated in 15. Twenty-five patients with hypoxia underwent an arterial-blood gas test. Assuming a normal arterial oxygen partial pressure to fractional inspired oxygen (PaO2/ FiO2 or P/F) ratio in the remaining 19 patients, a P/F ratio less than 260 was found in 10 patients. Thirteen patients did not receive any antiviral drug, while 31 patients received antiviral treatment (Table 1) . Antiviral treatment consisted in lopinavir/ritonavir (LPV/r) 200/50 mg twice a day plus hydroxychloroquine (HCQ) 200 mg twice a day. Antibiotic therapy was started in 32 patients and consisted of piperacillin/tazobactam and doxycycline. Supportive therapy was delivered based on clinical indications. In the univariate analysis for discharge, age > 65 years, interstitial pneumonia, presence of comorbidities, hypertension and antibiotic therapy were significantly associated with lower odds of discharge. In the univariate analysis for disease severity, male sex, respiratory frequency > 22, cancer as comorbidity, thrombocytopenia and arterial partial pressure of oxygen/fractional inspired oxygen (PF) ratio < 260 were significantly associated with higher odds for severe disease. LDH levels > 300 mU/mL and antiviral therapy were associated with both outcomes (Supplementary material, Table S1 ). As at 4 March 2020, 14 patients were discharged from the hospital. In these patients, median time from symptom onset was 12 days (range: 6-30 days, IQR: 6) and median time of hospitalisation was 6 days (range: 4-7 days, IQR: 2). Age over 65 years and antiviral treatment were significantly associated with lower odds of discharge, by multivariable analysis (Table 2) . Seventeen patients had severe disease and there were two fatalities. LDH levels > 300 mU/mL were significantly associated with higher odds of severe disease, by multivariable analysis (Table 2 ). In the supplementary materials we report detailed results of univariate and multivariable analysis. Particularly, Table S2 show results of model for discharge status, and Table S3 for severe disease. Table S4 reports the characteristics of patients who have received and who have not received antiviral treatment. Table S5 reports model for antiviral treatment. Eventually, Table S6 shows the characteristics of patients according to LDH levels. Two patients received sub-intensive care. In these patients, time from symptom onset was 10 and 13 days, respectively and time from hospitalisation was 5 days. Three patients were admitted to the intensive care unit. Time from symptom onset in these patients was 10, 12, 25 days, respectively and time from hospitalisation was 4 days in two and 5 days in one patient. The deaths occurred 9 and 10 days, respectively, after the onset of symptoms and 3 days after hospitalisation. As at 4 March, 23 patients were still in the isolation room receiving standard care, including standard supportive care. No bacterial infections were observed during the first week of observation. Two patients without known renal disease, developed kidney failure during hospital stay. Our early experiences from the COVID-19 pandemic showed significant morbidity in an aged population with high prevalence of coexisting illnesses. Older age and antiviral treatment were significantly associated with lower odds of hospital discharge; LDH levels > 300mg/dL were associated with higher odds of developing severe disease. It is important to underline that the median age and prevalence of coexisting illnesses observed in our cohort were higher than those reported in studies from China [8, 9] , potentially leading to a more severe clinical course. Similar to other studies, the most common comorbidities were hypertension, chronic heart disease and diabetes mellitus [10] . A high number of our patients received antiviral treatment with LPV/r plus HCQ. However, patients who received antiviral treatment had lower odds of discharge. This association appears plausible because of the propensity of physicians to administer antiviral treatment to patients with more severe disease. The actual role of therapy, and the optimal treatment schedule for COVID-19 are still unclear. Doubts were raised regarding the clinical effectiveness of LPV/r [11] and several clinical trials are underway to evaluate the efficacy of other novel antivirals (e.g. remdesivir) and selective cytokine blockade (e.g. tocilizumab) [12] . Based on findings in the literature, most of our patients received antibiotic combination therapy, to cover both common and atypical pathogens [8] . Remarkably, no bacterial superinfections were observed during the first week of observation. It is interesting to underline that among biological markers, heightened LDH levels were the only independent risk factors for severe disease. This finding was also reported in previous Chinese studies [8, 13, 14] as well as in an earlier study on Middle East respiratory syndrome (MERS) coronavirus [15] . It is well known that elevated LDH levels are suggestive of haematological malignancy and acute lung injury, such as Pneumocystis pneumonia (PCP) in HIV patients [16] . LDH levels might reflect tissue necrosis related to immune hyperactivity and thus relate to poor clinical outcome [17] . LDH levels may be a useful and easy to test parameter in order to identify patients at risk for severe respiratory failure. The lymphocyte count is a parameter that has been described as linked to prognosis of COVID-19. Our laboratory data showed a low lymphocyte count in a considerable proportion of patients, a finding confirming previous reports [18] [19] [20] . Our results support the use of low absolute value of lymphocyte counts as a reference index in the diagnosis of COVID-19 [8, 21] . Regarding the clinical characteristics of our cohort, fever was the most common symptom, this is similar to the results reported by a meta-analysis of studies performed in Chinese patients [22] . However, both cough and muscle weakness/fatigue were less frequent than in other studies. Although some studies demonstrated the presence of SARS-CoV-2 in stool samples [23] and that it might be associated with gastrointestinal symptoms [24] , nausea and diarrhoea were rare in our patients. Further studies are needed to assess if these differences reflect peculiar clinical manifestation of the disease in the Italian context. To date, more than 700 COVID-19 patients have been admitted to our hospital and further, more in-depth studies will follow. However, this picture of the first week of COVID-19 outbreak provides information regarding the disease outside China in a real life-setting of a country with very high case numbers. Although our study is limited by the small number of patients and by the short follow-up, our findings suggest that in a Caucasian aged population with high prevalence of coexisting illnesses, COVID-19 is characterised by notable morbidity. 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None declared. This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence and indicate if changes were made.Any supplementary material referenced in the article can be found in the online version.