key: cord-0818788-2c7p56s1
authors: Savelli, G.; Bonacina, M.; Rizzo, A.; Zaniboni, A.
title: Activated macrophages are the main inflammatory cell in COVID-19 interstitial pneumonia infiltrates. Is it possible to show their metabolic activity and thus the grade of inflammatory burden with (18)F-Fluorocholine PET/CT?
date: 2020-05-27
journal: Med Hypotheses
DOI: 10.1016/j.mehy.2020.109885
sha: ca3c2aefff5b7bf30c223da9eb2411ca0efd6ddc
doc_id: 818788
cord_uid: 2c7p56s1

The recent outbreak of Covid-19 has represented a major challenge for the countries affected by the disease, not only in terms of loss of human life, economic downturn, and constraint on individual freedom, but also for the great pressure on the national health systems and hospitals. The 380 kDa virus has been a perfect storm, especially for those national health systems used to working with limited resources and high intensity rhythms, such as Italy. For the first time in the new century, a virtually unknown fast-spreading disease has caused a public health emergency thus forcing most countries to deal with an insurmountable logistic gap. Hence, every branch of Medicine, even though not directly involved in the treatment, has been called upon to provide its contribution to resolve the crisis. It is now becoming more apparent that Covid-19 is not solely a lung disease, but a complex systemic disease involving several organs and systems. This is due to an abnormal inflammatory response which eventually leads to multisystemic coagulopathy which mainly, but not uniquely, targets the lungs. Although the pathophysiology of this syndrome is still not fully understood, macrophages and their immune complex system seem to play a key role. It is not yet clear why some patients develop the violent immune response which results in pneumonitis while others do not. There are clues indicating that the systemic hyper-inflammation defined as macrophage activation syndrome (MAS), or cytokine storm, requires an increase in choline consumption to synthesize phosphatidylcholine and stimulate phagocytosis, organelle biogenesis, secretory functions, and endocytosis. (18)F-Fluorocholine is a synthetic analog of the naturally occurring choline normally used for PET/CT imaging of prostate cancer patients. (18)F-Fluorocholine could image and quantify the macrophage activity in pulmonary interstitial infiltrates of Covid-19 pneumonia. If the hypothesis is confirmed experimentally, (18)F-Fluorocholine PET/CT could be used to in vivo image and quantify the degree of lung inflammation and potentially stratify the gravity of this disease.

such as Italy. For the first time in the new century, a virtually unknown fast-spreading disease has caused a public health emergency thus forcing most countries to deal with an insurmountable logistic gap. Hence, every branch of Medicine, even though not directly involved in the treatment, has been called upon to provide its contribution to resolve the crisis. It is now becoming more apparent that Covid-19 is not solely a lung disease, but a complex systemic disease involving several organs and systems. This is due to an abnormal inflammatory response which eventually leads to multisystemic coagulopathy which mainly, but not uniquely, targets the lungs. Although the pathophysiology of this syndrome is still not fully understood, macrophages and their immune complex system seem to play a key role. It is not yet clear why some patients develop the violent immune response which results in pneumonitis while others do not. There are clues indicating that the systemic hyper-inflammation defined as macrophage activation syndrome (MAS), or cytokine storm, requires an increase in choline consumption to synthesize phosphatidylcholine and stimulate phagocytosis, organelle biogenesis, secretory functions, and endocytosis. 18 F-Fluorocholine is a synthetic analog of the naturally occurring choline normally used for PET/CT imaging of prostate cancer patients. 18 F-Fluorocholine could image and quantify the macrophage activity in pulmonary interstitial infiltrates of Covid-19 pneumonia. If the hypothesis is confirmed experimentally, 18 F-Fluorocholine PET/CT could be used to in vivo image and quantify the degree of lung inflammation and potentially stratify the gravity of this disease.

The global pandemic which originated in China and rapidly spread to Europe and the U.S. is caused by Coronavirus disease 19 (Covid-19) whose main clinical feature is a severe acute respiratory syndrome: up to 20% of the patients affected by Covid-19 develop respiratory symptoms which require oxygenation 1 , 2 , 3 .

The urgency of finding a definition of the pathogenetic bases of the lung damage and establishing an effective therapy, together with insufficient medical information, has led clinicians to focus on alveolar injury characterized by altered alveolar lining cells, reactive type II pneumocyte hyperplasia, and intra-alveolar fibrinous exudates 4 

Phospholipids are the major component of macrophage cell membranes. The main phospholipid is phosphatidylcholine (PC), a chemical compound which incorporates choline. Once activated by an external noxa, the macrophages undergo the so-called polarization through the production of inflammatory cytokine and the onset of the pro-inflammatory phase 8 . The activation, coupled with changes in membrane composition and dynamics due to the induction of phospholipid biosynthesis (mainly PC), has been largely attributed to a demand for processes such as phagocytosis, organelle biogenesis, secretory functions, and endocytosis 9 . In summary, the activated macrophages therefore require more choline to adapt to the altered situation. lung. The patient underwent examination within his routine follow-up for a prostate adenocarcinoma. PSA was 1.97 ng/ml and unchanged compared with the previous evaluation. The CT scan shows diffuse wide areas of solid-subsolid ground-glass opacities, bilateral and subpleural crazy-paving bronchovascular thickening. The patient referred mild fever (>38 °C), cough, fatigue, shortness of breath, ageusia-dysgeusia and anosmia, all strongly suggestive symptoms for a Covid-19 infection. PET shows some mild-intense areas of uptake which follow, with irregular shape and intensity, the CT alterations.

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