key: cord-0809038-nr3fyyl0 authors: Engert, Viktoria; Siauw, Celine; Stock, Annika; Rehn, Monika; Wöckel, Achim; Härtel, Christoph; Wirbelauer, Johannes title: Severe Brain Damage in a Moderate Preterm Infant as Complication of Post-COVID-19 Response during Pregnancy date: 2021-06-14 journal: Neonatology DOI: 10.1159/000516846 sha: 661bc453de88dcfe7a419729d493a5d219a39357 doc_id: 809038 cord_uid: nr3fyyl0 Current evidence from the COVID-19 pandemic suggests that neonatal SARS-coronavirus-2 infections usually have a mild course. Data on how maternal infection during pregnancy affects fetal development are scarce. We present the unique case of a moderate preterm infant with intracranial bleeding and periventricular leukomalacia as a potential consequence of post-COVID-19 hyperinflammation during pregnancy. It is well acknowledged that infections during pregnancy with specific viruses, for example, cytomegalovirus, rubella virus, and zika virus, may represent major This is an Open Access article licensed under the Creative Commons Attribution-NonCommercial-4.0 International License (CC BY-NC) (http://www.karger.com/Services/OpenAccessLicense), applicable to the online version of the article only. Usage and distribution for commercial purposes requires written permission. Engert/Siauw/Stock/Rehn/Wöckel/ Wirbelauer/Härtel Neonatology 2 DOI: 10.1159/000516846 causes of permanent neurological disability in children. Current experience of the COVID-19 pandemic suggests that SARS-CoV-2 infection in pregnant women is associated with a higher risk for preterm delivery but usually mild or uneventful neonatal courses. Potential neurological manifestations of SARS-CoV-2 infection in the newborn are yet unclear [1] [2] [3] [4] [5] . Here, we report the complication of intracranial hemorrhage and periventricular leukomalacia in a moderate preterm infant born after maternal hyperinflammatory response following SARS-CoV-2 infection during the 2nd trimester of pregnancy. The 25-year-old mother presented at 33 weeks of gestation with preterm labor but no other clinical abnormalities. Apart from mild cold symptoms at 16 weeks of gestation, maternal history including immune-mediated and coagulation disorders was unremarkable. Laboratory evaluation revealed increased markers of inflammation, for example, C-reactive protein 14.5 mg/dL and a total white blood cell (WBC) count of 18.3/nL with normal platelet counts and coagulation studies. There was no focus for any infection. Due to pathological cardiotocography and maternal signs of inflammation, caesarean section was performed at 33 5/7 weeks. The histological examination of the placenta revealed no signs of funisitis, chorioamnionitis, or placental infarction. Microbiological evaluation of swabs (cervix and placenta) was negative for any pathogen. The preterm infant presented with Apgar score 4/7/7 after 1, 5, and 10 min (pH 7.28, base excess −5.0 mmol/L) and transitory tachypnea which was well controlled with respiratory support using continuous positive airway pressure. Ubiquitous petechial bleedings and pale hematomas were noticed on the skin but no other signs of clinical infection (shown in Fig. 1 ). Birth weight (2,100 g, 43rd percentile), length (45 cm, 48th percentile), and head circumference (30 cm, 21st percentile) were appropriate for gestational age. Day 1 cerebral ultrasound demonstrated cortical hyperechogenicity and cystic periventricular lesions (shown in Fig. 2 ). Laboratory studies revealed increased numbers of WBC (48.6/nL, nucleated red blood cells 27/100 WBCs), slightly increased procalcitonin (2.1 ng/mL, ref <0.5 ng/mL), but unremarkable C-reactive protein (<0.1 mg/dL). The initial hemoglobin value (17.9 g/dL, ref. 14.3-19 g/dL) was normal and the platelet count at the lower margin of reference values (132/nL, ref. 150-450/nL). Coagulation studies, however, were pathological, that is, Fig. 3 ). Maternal serology testing for SARS-CoV-2 (ELISA) revealed IgG antibodies against S1-protein (1. was also prevalent in the newborn serum, while specific anti-N-protein IgM and anti-S1/S2 IgM and IgG were negative. PCR test of SARS-CoV-2 after birth was negative in the mother (swab) and infant (swab and CSF). The infant recovered well and was discharged at 36 days of life without major clinical findings for further close follow-up. Our case report generates the hypothesis that a post-COVID systemic inflammatory response during pregnancy affected the fetal circulation followed by a coagulopathy and preterm labor. One proposed underlying mechanism might be the placental overexpression of angiotensin-converting enzyme 2 -the SARS-CoV-2 receptor -which can critically modulate hemodynamics within the uteroplacental unit [6] . Second, the proinflammatory cytokine storm invoked by SARS-CoV-2 may induce even more severe inflammation with deleterious consequences on the fetal brain during a critical time frame (e.g., development of periventricular leukomalacia). Further mechanistic links exist between hyperinflammation, endothelial activation, and dysregulated complement function which are known pathways to mediate microvascular injury, vasculitis, and coagulopathy [7] [8] [9] . Systemic inflammatory responses and coagulation disturbances are known complications of acute SARS-CoV-2 infection in intensive care patients [10, 11] . Hyperinflammatory responses also occur with temporal delay of several months after SARS-CoV-2 infection, for example, multisystem inflammatory syndrome in children. With a predominance in the first decades of life, post-COVID hyperinflammation is rather related to antibody-dependent enhancement of acquired immune responses to virus than direct virus replication. In the case of a 16-year-old boy, vascular inflammation was reported including stenosis of cerebral vessels leading to stroke [8] . In the specific context of immunotolerance during pregnancy, neonatal consequences of post-COVID hyperinflammation have not been described yet. In our unique case, diagnostic signs of inflammation and coagulopathy were intracranial and petechial bleeding (without abnormal platelet count) as well as highly elevated D-dimers and WBC count. Two other case reports describe neurological symptoms in newborns in relation to a SARS-CoV-2 infection Sagittal T2-weighted image shows intracerebral hemorrhage with sedimentation phenomenon (arrow) (a); blood degradation products were also found in the subarachnoid spaces (arrow) on susceptibility-weighted images (b) and subdural on the left side, exemplary on T1-weighted images (c). due to perinatal transmission. The newborns presented with encephalitic symptoms such as lethargy, irritability, axial hypertonia, and high-pitched crying but unremarkable CSF results. Cerebral imaging was normal in 1 affected infant, while white matter injury was diagnosed in the other case being suggestive for cerebrovascular inflammation [12, 13] . To our knowledge, we present the first report of severe brain damage as a potential consequence of SARS-CoV-2-mediated hyperinflammation during pregnancy. We propose that the current pandemic situation requires serological screening of mother-infant dyads for SARS-CoV-2 antibodies when unusual clinical appearances of vasculitis, inflammation, or coagulopathy are obvious. Furthermore, all infants of mothers with known SARS-CoV-2 infection during pregnancy should be monitored for adverse neurological findings. 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Arch Pediatr Neonatal early-onset infection with SARS-CoV-2 in a newborn presenting with encephalitic symptoms Transplacental transmission of SARS-CoV-2 infection Virological diagnostics was performed by Dr. Benedict Weißbrich, Institute of Virology, University of Würzburg. The parents have given their written informed consent to publish the case including publication of images. The authors have no conflicts of interest to declare. There is no particular funding to the manuscript. V.E., J.W., and C.H. drafted the first version of the manuscript. C.S., M.R., A.W., V.E., J.W., and C.H. were involved in the treatment of mother and infant. A.S. performed MR imaging and interpreted imaging finding. All authors have read and critically revised the final version of the manuscript.