key: cord-0808165-pgda7i3u authors: Baba, Yuri; Ishiguro, Takashi; Gochi, Mina; Shimizu, Yoshihiko; Takayanagi, Noboru title: A 72-Year-Old Woman With Respiratory Failure and Bilateral Ground-Glass Opacities date: 2020-07-02 journal: Chest DOI: 10.1016/j.chest.2019.11.054 sha: 2cc2901fb4c3263a5f1961b8e1bec68d963d574d doc_id: 808165 cord_uid: pgda7i3u A 72-year-old woman with diabetes mellitus was admitted to our hospital because of dyspnea on exertion. Sputum cytologic evaluation revealed intranuclear inclusion bodies in the cells; we therefore considered viral pneumonia and performed a bronchoscopy. The bronchial washing fluid was positive for immunoperoxidase staining of herpes simplex virus type 1 (HSV1) and HSV1 polymerase chain reaction. The patient was diagnosed as having pneumonia due to HSV1 and was successfully treated with acyclovir. HSV1 polymerase chain reaction. The patient was diagnosed as having pneumonia due to HSV1 and was successfully treated with acyclovir. CHEST 2020; 158(1):e41-e45 KEY WORDS: bronchoscopy; herpes simplex virus pneumonia; immunoperoxidase staining A 72-year-old Japanese housewife was admitted to our hospital due to anorexia and dyspnea on exertion. She had been diagnosed several years earlier with type 2 diabetes mellitus and hypertension. She had also developed lip herpes several years ago. She had no history of smoking or drinking. She had been anorexic for 5 days before and developed dyspnea on exertion from 3 days prior to referral and admission to our hospital. She had had no contact with any people showing infectious symptoms prior to her presentation, and she had not been started on any new medications for the past few years. On admission, the patient's body temperature was 37.6 C, and oxygen saturation measured by pulse oximetry under inhalation of oxygen at 3 L/min by nasal cannula was 93%. Her lips and pubic region showed no exanthema. Auscultation revealed diffuse fine crackles. Her peripheral limbs were not edematous. A chest radiograph showed bilateral patchy opacities distributed predominantly in the upper lung fields (Fig 1A) . No pleural effusion or obvious lymphadenopathy was observed. Chest CT images obtained during inspiration revealed multifocal patchy ground-glass opacities (Fig 1B) , bronchial wall thickening, and interlobular septal thickening but no pleural effusion or lymphadenopathy. Laboratory data on admission showed a WBC count of 8,800/mm 3 with infectious symptoms, or a history suggestive of drug-induced lung diseases but did have fine crackles and diffuse, bilateral patchy ground-glass opacities in her chest, 1 we initially suspected acute interstitial pneumonia and administered prednisolone (50 mg/d), which is often used in the treatment of acute interstitial pneumonia. Because we could not completely rule out community-acquired pneumonia due to atypical pathogens, we administered azithromycin 2 g on hospital day (HD) 1. On HD 3, the shadows on the patient's chest radiograph increased. We therefore changed the steroid therapy to pulse therapy (methylprednisolone 1 g daily for 3 days). Her respiratory condition continued to worsen, however. What Study Should Be Conducted Next? On HD 7, we began to suspect viral pneumonia because of a sputum cytology finding of intranuclear inclusion bodies in the cells (Fig 2A) . Because of the patient's worsening respiratory condition, she was intubated, and a bronchoscopy was then performed to obtain a definitive diagnosis. Cytologic evaluation of the bronchial washing fluid obtained from the right upper lobe revealed intranuclear inclusion bodies ( Fig 2B) . Results of culture of the bronchial washing fluid were negative for bacteria, fungus, and mycobacteria. Immunoperoxidase staining for herpes simplex virus type 1 (HSV1) of lymphocytes using bronchial washing fluid was positive (Fig 2C) Diagnosis: Viral Pneumonia due to HSV1 The patient was diagnosed as having HSV1 pneumonia (HSV1P) based on these results. On HD 11, she was started on acyclovir 15 mg/kg/d for 16 days. The bilateral shadows on the patient's chest radiograph then improved. Serum anti-HSV1 IgG measured by using an enzyme-linked immunoassay method and complement fixation method was > 128 times normal and 32 titers on admission, respectively, and her HSV1 IgG titers had remained high. HSV1 IgM was negative at all measurements. After discharge on HD 62, the patient has continued to be followed up as an outpatient and has not developed relapse of HSV1P. The bacterial pathogens of pneumonia have mainly been identified on the basis of culture, paired sera, and rapid diagnostic test results. PCR testing directed at respiratory viruses has been reported to find viruses more frequently than had been previously thought. [2] [3] [4] [5] These studies investigated influenza virus, respiratory syncytial virus, Figure 1 -A, Chest radiograph on admission showed bilateral patchy opacities distributed predominantly in the upper lung fields. Pleural effusion and lymphadenopathy were not observed. B, Chest CT image on admission showed multifocal segmental and subsegmental ground-glass opacities, bronchial wall thickening, and interlobular septal thickening but no pleural effusion or lymphadenopathy. e42 Chest Imaging and Pathology for Clinicians coronavirus, human metapneumovirus, and adenovirus, but HSV was not included in previous reports investigating the frequency of viral infection in community-acquired pneumonia. HSV1P is rare and mostly observed in transplant recipients and those receiving immunosuppressants or steroids. As far as we know, the number of reports of HSV1P is limited to 10 cases of patients unaffected by transplantation, HIV, immunosuppressants, corticosteroids, burns, or malignancy 6-14 (Table 1) . Unfortunately, although we did not initially suspect HSV1P in the current patient, cytologic findings of sputum provided a diagnostic clue, and we subsequently investigated HSV infection. The findings obtained following bronchoscopy were very helpful in diagnosing HSV1P. HSV can be cultured from the oral cavity of 1% to 5% of asymptomatic adults, and it is difficult to identify HSV as a pathogen of pneumonia when the virus is cultured from the sputum. 15 To confirm the diagnosis of HSV1P, it is important to prove that HSV is positive by using PCR and according to typical cytologic and histologic findings from the lower respiratory tract and alveoli. [15] [16] [17] Among the 10 reported patients with HSV1P unaffected by transplantation, HIV, immunosuppressants, corticosteroids, burns, or malignancy ( Table 1) , eight of the 10 were aged < 50 years, and seven had no underlying diseases. Only the current patient had diabetes mellitus with mild elevation of her glycosylated hemoglobin value, which made it difficult for us to suspect HSV1P. Therefore, although HSV1P is rare, physicians should consider it in the differential diagnosis of patients with no underlying diseases or a fragile immunologic state. A noteworthy fact is that no skin, oral, or genital lesions suggestive of HSV infection were found in any of the patients with HSV pneumonia. HSV has two patterns of acute infection: primary infection and reactivation. The infection pattern in the current patient was considered to be reactivation because her anti-HSV1 IgG level was high, 18 and she had experienced a herpes virus lip infection several years earlier. Acyclovir was generally selected for the treatment of HSV1P, [6] [7] [8] [9] [10] [11] [12] [13] [14] 19 except in two cases ( Table 1 ). The study patient improved spontaneously with no medications, 6 and a postmortem diagnosis of HSV1P was made in the other patient. One study reported a mean treatment duration of 9 AE 3 days in immunocompetent patients, whereas immunocompromised patients were treated with acyclovir for 17 AE 10 days. 20 Acyclovir was administered to the study patient for 16 days, and there has been no relapse of HSV1P. Few comments are available regarding events following recovery from HSV1P; however, one patient developed acute inflammatory demyelinating polyneuropathy (a variant of Guillain-Barré syndrome) following improvement with acyclovir, 10 which should alert physicians to potentially serious outcomes. Another concern is corticosteroids, which we initially administered for a suspected diagnosis of acute interstitial pneumonia. Limited data suggest favorable effects of corticosteroids on varicella zoster virus (in combination with acyclovir), hantavirus, 21 and in influenza-associated pneumonia in some clinical settings, 22,23 but other reports have found them to be harmful. 24 The study patient's condition clearly worsened following corticosteroid therapy. Patients with acute interstitial pneumonia report progressive dyspnea, cough, fever, and, occasionally, flu-like symptoms, which overlap with the symptoms of viral pneumonia. Careful attention should be paid in the differentiation of viral pneumonia prior to administering corticosteroids. Cytologic features characteristic of HSV infection can be found at the margins of ulcers or in the alveolar cells, and they include small eosinophilic intranuclear inclusion bodies separated from the surrounding nuclear chromatin by a clear halo (Cowdry type A inclusions) and a single or multinucleated cells with ground-glass changes in the involved nuclei. 15, 25 The study patient exhibited these findings, which were compatible with HSV infection. We initially did not suspect HSV1P, but screening of sputum cytology samples provided a clue for correcting our strategies for diagnosis and treatment. The chest radiograph findings of HSV1P have been described in a few reports. Most described bilateral consolidation, but nodules with irregular margins and ground-glass opacities have also been reported. 15 CT findings of HSV1P include multifocal segmental and subsegmental ground-glass opacities and consolidation, scattered distribution, and pleural effusion, 25 which, except for pleural effusion, were also found in the study patient. Furthermore, interlobular septal thickening and bronchial thickening have been reported in viral pneumonia 26 and were also found in the current patient. Teaching Points 1. HSV1 virus is a cause of community-acquired pneumonia. 2. Although HSV1P is considered to be rare, it can develop in patients with diabetes mellitus with a mild increase in glycosylated hemoglobin levels and without any complications of diabetes mellitus itself. 3. When HSV1P is suspected, investigation of samples obtained from the lower respiratory tract is recommended. 4. No skin, oral, or genital lesions suggestive of HSV infection were found in any of the patients with reported HSV1P unaffected by transplantation, HIV, immunosuppressants, corticosteroids, burns, or malignancy. 5. Hasty administration of corticosteroids may be harmful to patients with viral pneumonia, and thus it is important to rule out viral pneumonia prior to the administration of corticosteroids. 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