key: cord-0805326-6pi0dq9e authors: Yeleti, Ramya; Guglin, Maya; Saleem, Kashif; Adigopula, Sasikanth V; Sinha, Anjan; Upadhyay, Smrity; Everett, Jeffrey E; Ballut, Kareem; Uppuluri, Sarada; Rao, Roopa A title: Fulminant myocarditis: COVID or not COVID? Reinfection or co-infection? date: 2021-02-22 journal: Future cardiology DOI: 10.2217/fca-2020-0237 sha: a8e41c996e2ef744ecc1768eb8ebc8cfffb3cfe9 doc_id: 805326 cord_uid: 6pi0dq9e We describe a unique case of fulminant myocarditis in a patient with presumed SARS-CoV-2 reinfection. Patient had initial infection 4 months backand had COVID-19 antibody at the time of presentation. Endomyocardial biopsy showed lymphocytic myocarditis, that is usually seen in viral myocarditis. The molecular diagnostic testing of the endomyocardial biopsy for cardiotropic viruses was positive for Parvovirus and negative for SARS-CoV-2. Authors highly suspect co-infection of SARS-CoV-2 and Parvovirus, that possibly triggered the immune cascade resulting in fulminant myocarditis. Patient was hemodynamically unstable with ventricular tachycardia and was supported on VA ECMO and Impella CP. There was impressive recovery of left ventricular function within 48 hours, leading to decannulation of VA ECMO in 72 h. This unique case was written by the survivor herself. After initial stabilization on mechanical support, intravenous methylprednisolone 1 gm was started on day 1. On day 2, moderate pericardial effusion (Supplementary Video 1) was noted, and pericardial window was done. At this time COVID test results came back positive along with positive antibody. Due to the severity of the hemodynamic compromise, she was treated with Remdesivir and convalescent plasma. Echocardiogram done the very next day (48 hours of presentation) showed impressive recovery of biventricular function with near normal LVEF (Supplementary Video 2). After another 24 hours of hemodynamic stability, patient was successfully decannulated from VA ECMO and was maintained on Impella support alone. The next day transesophageal echocardiogram was done prior to the removal of the Impella. Severe mitral regurgitation was noted due to the flail and torn A3 component of anterior mitral valve. Mitral valve repair was immediately performed with 6 gortex neo-chords to A3 of anterior mitral valve and annuloplasty ring. Separation from cardiopulmonary bypass was delayed due to biventricular failure, however there was impressive recovery of LVEF to normal once again in the operating room. Patient was extubated the next day. Cardiac MRI (CMR) done prior to discharge showed transmural late gadolinium enhancement of basal-mid anterolateral and inferolateral segments ( Figure 3 ). Patient was continued on high dose steroids and was discharged to home in stable condition and normal LVEF (Supplementary Video 3) on day 13. At the 4-month follow-up, the patient continues to do well on medical therapy with preservation of LVEF. Currently, she is on a slow steroid wean. This case is unique for various reasons. SARS-CoV-2 PCR was positive after 4 months of initial infection, raising the possibility of reinfection. According to CDC patients can get reinfection after 3 months of initial infection. However, the patient also had Positive SARS-CoV-2 IgG antibody at the time of presentation. SARS-CoV-2 IgG antibody is thought be highly protective in preventing reinfection. Ours is the first case to isolate Parvovirus genome in the myocardial tissue in a patient with COVID-19. FM as the initial presentation of SARS-CoV-2 infection, requiring ECMO for hemodynamic support is rare. The other distinctive features include rapid recovery of FM within 48 hours of presentation and ECMO decannulation in 72 hours. This case report is quite unique as one the authors of the case report is the survivor herself. Cardiovascular involvement from SARS-CoV-2 although is widely reported, in general is uncommon. FM in COVID patients has been limited to case reports [1] . The exact mechanism of myocarditis in COVID patients is not clear. SARS-CoV-2 is not thought to be a cardiotropic virus [2] . Isolation of the virus from the cardiomyocyte in the autopsy or biopsy specimen has not been possible [2] . However viral particles have been found in cardiomyocyte in one pediatric patient [3] and in the myocardial macrophage in another case [4] . Alternatively, virus can trigger inflammatory response in the form of SIRS, lymphopenia, macrophage activation and cytokine storm releasing IL-6, IL-10 and TNF-α which can cause myocarditis [5] . Co-infection with another virus or bacteria is a known common phenomenon resulting in myocarditis. Ours is the first case to demonstrate parvovirus in the endomyocardial biopsy in a patient otherwise with presumed COVID myocarditis. Parvovirus is a cardiotropic virus and FM has been described [6] . Shah et al. noticed high coinfection rate (22.4%) in COVID-19 patients [7] . Probably we can postulate that infection with SARS-CoV-2 and Parvovirus co-infection triggered immune cascade and lead to FM. In this patient IgM for parvo virus was negative and IgG was positive indicating past infection. IgG antibody production usually occurs 18-24 days after the infection and can persist for years. It is not very clear if the patient contracted reinfection as she was persistently positive for PCR in the presence of SARS-CoV-2 antibody. However, it is currently not known if or how antibody testing results correlate with immunity to SARS-CoV-2 infection. COVID reinfection is being reported as early as 4 weeks from initial infection and the second infection can be more severe [8, 9] . Alternatively, patients can persistently test positive despite clearing the infection, partly due to presence of viral particles. There is a prevalent theory of a link between acute myocarditis (AM) and autoimmune disease; the association is seen in about 7.2% of AM patients [10] . The patient was positive for anti-Ro antibodies, had significant inflammation on CMR and had history of vitiligo which can be associated other autoimmune conditions. These factors were taken into consideration in initiating glucocorticoid therapy. Since her LVEF recovered rapidly, concomitant therapy with azathioprine, cyclosporine and Mycophenolate mofetil were not considered due to lack of convincing evidence from a risk versus benefit analysis. Notably, the patient received convalescent plasma immunoglobulin during her stay. Research regarding Immunoglobin as AM treatment is well described in children. The Immunoglobulin therapy may have a role in AM as cardiac autoantibodies are frequently seen in myocarditis. One may consider using Immunoglobulin therapy especially in hemodynamically unstable patient as a treatment modality [11] . FM with cardiogenic shock can be expected in COVID patients and requires a full extent of hemodynamic support along with anti-viral treatment. This case raises the impact of possible co-infection in COVID patients. This case also highlights the diagnostic challenges that we face in the era of pandemic. To view the supplementary data that accompany this paper please visit the journal website at: www.futuremedicine.com/doi/sup pl/10.2217/fca-2020-0237 Financial & competing interests disclosure COVID-19 fulminant myocarditis: a case report Clinically suspected myocarditis in the course of severe acute respiratory syndrome novel coronavirus-2 infection: fact or fiction? Eur • Describes the various COVID myocarditis cases in the literature SARS-CoV-2 in cardiac tissue of a child with COVID-19-related multisystem inflammatory syndrome • First paper to report SARS-Co-V-2 viral particle inside the cardiac myocyte Myocardial localization of coronavirus in COVID-19 cardiogenic shock • Paper reporting SARS-CoV-2 viral particle in the macrophage in the endomyocardial biopsy Recognizing COVID-19-related myocarditis: the possible pathophysiology and proposed guideline for diagnosis and management • Describes the possible mechanisms of COVID-19 related myocarditis Parvovirus B19 Myocarditis of Fulminant Evolution Higher co-infection rates in COVID19 Genomic evidence for reinfection with SARS-CoV-2: a case study COVID-19 re-infection by a phylogenetically distinct SARS-CoV-2 variant, first confirmed event in South America Clinical presentation and outcome in a contemporary cohort of patients with acute myocarditis The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.No writing assistance was utilized in the production of this manuscript. The authors state that they have obtained verbal and written informed consent from the patient/patients for the inclusion of their medical and treatment history within this case report.