key: cord-0800246-5evr2v3r
authors: Shental, Noam; Levy, Shlomia; Wuvshet, Vered; Skorniakov, Shosh; Shalem, Bar; Ottolenghi, Aner; Greenshpan, Yariv; Steinberg, Rachel; Edri, Avishay; Gillis, Roni; Goldhirsh, Michal; Moscovici, Khen; Sachren, Sinai; Friedman, Lilach M.; Nesher, Lior; Shemer-Avni, Yonat; Porgador, Angel; Hertz, Tomer
title: Efficient high-throughput SARS-CoV-2 testing to detect asymptomatic carriers
date: 2020-09-11
journal: Sci Adv
DOI: 10.1126/sciadv.abc5961
sha: eba0593ffbc96b9616225a7665e240c7f6c4b6b5
doc_id: 800246
cord_uid: 5evr2v3r

Recent reports suggest that 10 to 30% of severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) infected patients are asymptomatic and that viral shedding may occur before symptom onset. Therefore, there is an urgent need to increase diagnostic testing capabilities to prevent disease spread. We developed P-BEST, a method for Pooling-Based Efficient SARS-CoV-2 Testing, which identifies all positive subjects within a set of samples using a single round of testing. Each sample is assigned into multiple pools using a combinatorial pooling strategy based on compressed sensing. We pooled sets of 384 samples into 48 pools, providing both an eightfold increase in testing efficiency and an eightfold reduction in test costs, while identifying up to five positive carriers. We then used P-BEST to screen 1115 health care workers using 144 tests. P- BEST provides an efficient and easy-to-implement solution for increasing testing capacity that can be easily integrated into diagnostic laboratories.

The coronavirus disease 2019 (COVID-19) pandemic is rapidly spreading throughout the world. Recent reports suggest that 10 to 30% of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected patients are asymptomatic (1) (2) (3) (4) (5) . Other studies report that some subjects have substantial viral shedding before symptom onset (6) . There have been multiple recent studies that identified asymptomatic carriers and have attempted to estimate their rate and contribution to disease spread. A study of 2685 tourists in the New York area conducted over two seasons found that 6.2% of subjects tested positive for at least one respiratory virus, and 38.7% of these were infected with circulating human corona viruses (7) . Rothe et al. (5) described transmission of SARS-CoV-2 from a German patient who was infected by a Chinese businesswoman who visited Germany. Two other German co-workers were infected but only came in contact with the German patient who was asymptomatic. Mizumoto et al. (3) reported that 50.5% of infected patients on board the Diamond Princess cruise ship were asymptomatic at time of diagnosis. Using a model, they found that the estimated asymptomatic proportion (among all infected cases) was 17.9% [95% confidence interval (CI), 15.5 to 20.2%]. A study of evacuated Japanese nationals from Wuhan China, estimated that 30.8% of subjects were asymptomatic (95% CI, 7.7 to 53.8%) (8) . A recent modeling study estimated that COVID-19 may be most transmissible in the 2 days before symptom onset (9) . Another recent study of 3184 infections in Japan identified 61 case clusters at various public events (10) . Since both asymptomatic and presymptomatic subjects can spread the disease (1, 2) , identifying these individuals is critical for effective control of the SARS-CoV-2 pandemic.

A major bottleneck of managing the COVID-19 pandemic in many countries is diagnostic testing, which is performed primarily on symptomatic patients, because of limited laboratory capabilities and limited access to genome-extraction and polymerase chain reaction (PCR) reagents. On the other hand, there is an urgent need to increase diagnostic testing capabilities to allow screening of asymptomatic and presymptomatic populations. These tests will be routinely required until a vaccine is developed.

Here, we developed P-BEST, a method for Pooling-Based Efficient SARS-CoV-2 Testing, using a single-stage nonadaptive group-testing approach, which significantly reduces the number of tests required to identify all positive subjects within a large set of samples. P-BEST can be configured on the basis of the carrier rate of a given population, and as we show below, if the carrier rate is below 1.3%, then the method provides both an eightfold improvement in testing efficiency and an eightfold reduction in test costs.

To reduce the number of tests required for identifying all carriers, P-BEST uses a single-step (nonadaptive) group-testing design. Instead of testing each sample separately, samples are pooled into groups, and each pool is tested for SARS-CoV-2 using a standard clinically approved PCR-based diagnostic assay. Each sample is part of multiple pools, using a combinatorial pooling strategy designed for maximizing the ability to identify all positive individuals (11, 12) . PCR results for each of the pools are provided to the detection algorithm (see Methods), which identifies all positive carriers without the need for an additional testing stage (Fig. 1 , A to C). Pooling is performed on samples before RNA extraction. The efficiency of group testing (i.e., the ratio of the number of samples to the number of pools) is affected by the positive carrier rate of the population tested. As the carrier rate increases, the efficiency is reduced, since more pools are required to correctly identify all positive subjects. If the percentage of carriers in the tested set of samples is ~1%, then our method can correctly identify all positive individuals using an eightfold reduction in the number of diagnostic tests as compared to testing each individual sample separately.

We tested P-BEST using a pooling design constructed for carrier rates of up to ~1% (i.e., four positive carriers within a set of 384 samples). We evaluated P-BEST using leftover samples that were previously clinically tested for COVID-19. A total of 384 samples diluted in lysis buffer were pooled into 48 pools, each containing a set of 48 unique samples. Pooled samples were then tested by the clinical diagnostic laboratory of the Soroka University Medical Center (SUMC) using a clinically approved COVID-19 PCR-based diagnostic protocol that included an RNA extraction stage. We tested P-BEST using four sets of 384 samples, each containing an increasing number of positive carriers ranging from two to five. We found that P-BEST was able to correctly identify all positive carriers within these four sets of 384 samples using only 48 tests per set, providing an eightfold increase in testing efficiency (Fig. 1D) . Only in the case of five carriers (carrier rate of 1.3%), one additional false-positive carrier was detected.

A key issue with sample pooling is loss of sensitivity due to sample dilution. In general, pooling a positive sample with additional negative samples will result in dilution of the viral RNA concentration within the pool. Theoretically, pooling eight samples will result in a reduction of ~3 PCR cycles, and pooling 16 samples will lead to a reduction of ~4 PCR cycles, etc. Since in P-BEST, samples are diluted into pools of 48 subjects, there is an inherent drop in PCR sensitivity of about five to six cycles (a factor of 2 5 to 2 6 ), which was observed experimentally. In retrospective analysis, we found that only a single pool that included a carrier yielded a negative PCR result (of 70 positive pools across four experiments). Similarly, only 1 of 122 of the negative pools was positively identified by the PCRbased assay. These two errors had no effect on the detection capabilities of P-BEST, which is robust to both of these types of errors.

To test the effect of pool size on PCR sensitivity, we conducted two independent experiments in which positive samples were diluted into sets of negative samples. In our first experiment, a set of five positive samples {with cycle threshold [C(t)] values ranging from 26.2 to 34.2} was individually mixed into a set of 7, 15, 19, and 23 negative samples generating pools of size 8, 16, 20, and 24 samples, respectively. Each single sample and pools were then tested for SARS-Cov-2 using an in-house PCR kit based on the E gene. We found that all pools were PCR positive, yet in some cases, larger pool size actually yielded a lower C(t) ( Fig. 2A) . In a second experiment, another set of five samples [with C(t) values ranging from 25.2 to 30.8] was mixed with three distinct sets of 15 and 47 negative samples, resulting in pools of sizes 16 and 48, respectively. RNA was extracted from each single sample and pools and was then tested for SARS-CoV-2 using the Seegene COVID-19 diagnostic kit. We found that all five samples were positively detected in all of the pools of both sizes 16 and 48 (Fig. 2B ). The level of variability within each triplicate differed by sample.

The performance of P-BEST was also evaluated using in silico simulations. In each simulation, a certain number of carriers (ranging from one to five) were randomly selected within sets of 384 samples, and a P-BEST experiment was simulated using the reported pooling design. Simulations included two sources of noise: (i) PCR failure, which can lead to dropped pools; and (ii) liquid dispensing volume errors corresponding to 50% error in the amount of RNA. In each such simulation, we quantified the number of false negatives, false positives, and true positives returned by our method. Simulations demonstrated that the method can correctly identify up to 5 of 384 (1.3%) carriers, with an average number of false positives that was less than 2.75 and an average number of false negatives that was less than 0.33 (Fig. 3) .

To test the robustness of P-BEST, we considered two types of potential noise factors. First, variation in initial RNA levels may 

We are conducting an ongoing clinical study to screen asymptomatic health care personnel of the SUMC using P-BEST. Thus far, we have approached 1118 health care employees; of these, 1115 agreed to participate and signed an informed consent form. Within the cohort, 690 (62%) were female and 425 (38%) were male. Subjects were recruited across all SUMC staff and included physicians (n = 165, 14.8%), nurses (n = 157, 14.1%), nurse assistants (n = 43, 3.9%), other clinical staff (n = 119, 10.7%), and administrative staff (n = 631, For each pool size, the set of negative samples used was identical across all five samples tested, e.g., the same seven negative samples were used to create all pools of size 8. Sample's de-identified study IDs appear above each subplot. Each pool size is plotted in a different color. All samples, including those with C(t) > 34 were identified in all pool sizes, yet in some cases, the C(t) value does not monotonically increase with pool size. Samples were tested using an in-house PCR kit based on the SARS-CoV-2 E gene. (B) Five positive samples were mixed into three pools of sizes 16 and 48, each containing a distinct set of negative samples. Sample de-identified study IDs appear above each subplot. RNA was extracted from all single samples and pools and subsequently tested for SARS-CoV-2 using the Seegene diagnostic kit. All five samples were identified across all pools of both sizes, yet triplicates sometime display variation in C(t).

A small number of carriers were randomly assigned to 384 samples, and a P-BEST experiment was simulated using the reported pooling scheme designed for a carrier rate of ~1%. Simulations accounted for the estimated variation in RNA amounts based on measurements of n = 48 individual samples and also assumed that 1 of the 48 pools failed PCR amplification. Samples reported by P-BEST were compared to the true simulated sample labels to estimate the P-BEST's success rate. Results were averaged over 3000 simulations. Error bars correspond to 95% CIs. 56.6%). A total of 296 (26.5%) subjects worked in direct contact with patients with COVID-19. Within the cohort, 926 (93.1%) participants reported themselves as totally asymptomatic, 71 (6.3%) reported a mild cough, and 70 (6.3%) reported rhinorrhea. The 1115 participants were tested using three rounds of P-BEST using a total of 3 × 48 testing kits. All of the pools tested were negative. Because of the increasingly lower carrier rates in Israel during April 2020, the third batch was blindly spiked with a sample from a patient with COVID-19. As in our preliminary validation studies, we correctly identified this patient using the P-BEST method.

Here, we described P-BEST, a novel method for efficient SARS-Cov-2 diagnostic testing that is based on single-stage nonadaptive group testing. Using a pooling scheme designed for a carrier rate of ~1%, we showed that our method correctly identified all positive carriers in sets of 384 samples pooled into 48 pools, thereby providing an eightfold reduction in the number of required tests.

Any long-term mitigation strategy that will allow our society to exit from stringent lockdowns will require significant increases in testing capacity (13, 14) . While it is clear that in many regions around the world, the current carrier rates are substantially higher than 8%, the lockdown periods imposed in many places are slowly reducing infection rates. Therefore, we anticipate that in the near future, carrier rates are likely to drop below this threshold in many countries. P-BEST may then be used for conducting efficient routine screens of, e.g., health care workers and staff in nursing homes, as well as general population screens to identify new hotspots of the SARS-CoV-2.

Recently, several studies reported preliminary results using adaptive two-stage group-testing methods for SARS-CoV-2 testing (13, 15, 16) . Additional reports have analyzed the efficiency gain of implementing pooled testing (14, 17, 18) . Furthermore, the U.S. Food and Drug . S1 ). Simulations used the pooling scheme designed for a carrier rate of ~1%. The false-positive (left) and false-negative (right) detection rates for the two scenarios show that RNA variation does not substantially degrade P-BEST performance. All simulations considered one dropped pool. Error bars correspond to 95% CIs. To assess the effects of dropped pools due to PCR amplification failures, we measured the average number of false-positive (left) and false-negative (right) detections as a function of the true number of carriers across 3000 simulations for zero, one, or two randomly dropped pools using the pooling scheme designed for a carrier rate of ~1%. P-BEST seems to be robust to one to two dropped pools. All simulations considered the experimental level of RNA variation, as measured across 48 samples ( fig. S1A ). Error bars correspond to 95% CIs.

Administration recently published novel guidelines on sample pooling (19) . A clear advantage of P-BEST over other recently proposed pooling methods for SARS-CoV-2 diagnostic testing (20, 21) is that our method is a nonadaptive single-stage method. Carriers are detected using a single round of testing, which reduces the overall test time and does not require storing samples for a second round of testing. The latter is highly attractive for high-throughput diagnostic laboratories for which logistical simplicity is imperative. Recently, another method for single-stage COVID-19 group testing was suggested (22) .

Our current implementation of P-BEST was designed for a carrier rate of ~1%. To allow higher testing efficiency, i.e., to minimize the number of pools required to screen a given population, designs should vary according to the carrier rate. Pooling designs can differ by the number of pools and by the number of samples per pool. Specifically, as the carrier rate in the population rises, more pools are required to correctly identify all positive carriers in a single testing round. Moreover, the required number of samples per pool decreases with increasing the carrier rate. Conversely, when carrier rates are low, more efficient pooling designs can be used, i.e., less pools are required to test the same number of individuals, while the number of samples per pool should be increased. As the pandemic continues to spread, the infection rates within a given population are continuously changing. To provide a close to optimal pooling design, a rough estimate of the current carrier rate is required. In general, this rate can be estimated on the basis of the carrier rates observed during the last few days for a given population, since they typically gradually vary over time.

Each pooling design can correctly identify up to a certain percentage of carriers for which it was optimized. Therefore, carrier rates lower than the expected rate would not hamper detection (yet higher efficiency could have been achieved had this been known in advance). In contrast, the scenario in which the number of carriers is higher than expected may be problematic. As we show in our simulation results above, if the actual carrier rate ends up being slightly higher than the expected rate, then our pooling method will identify all positive carriers, as well as some additional false positives, without the addition of false negatives. Such graceful degradation is required to handle variations in carrier rate that are either real changes or a result from the small sample size tested in each pooling run. Cases where the actual carrier rate is much higher than the expected level may also be identified using our method. In this case, too many pools would be PCR positive, and the number of samples identified by the method will be much larger than the number of expected carriers. While this prevents the direct identification of the actual positive carriers using a single testing round, it will notify the user and allow him/her to retest the samples either individually or by using an alternate pooling design suited for a higher carrier rate.

As mentioned above, when carrier rates are sufficiently low, a significant increase in testing efficiency may be obtained, while requiring increasing the number of samples in each pool. However, increasing the pool size will lead to loss of sensitivity, and the optimal pool size should be quantified for each diagnostic kit. While two recent reports tested pooling sensitivity with pools of up to 32 samples per pool (13, 23) , we found that samples with C(t) < 35 were detected in pools of up to 48 samples each, using two different SARS-CoV-2 diagnostic tests. The loss of sensitivity differed from sample to sample and generally adhered to the theoretically expected value; however, this was not always the case. We note that P-BEST can be used with any pool size, yet limiting the pool size for low carrier rates will reduce its efficiency. For example, screening 384 samples using pools of size 16 (instead of 48) will require 72 pools, thereby providing a ~5.3-fold increase in testing efficiency (as compared to an eightfold increase with pools of 48).

We tested the performance of P-BEST in a clinical study aimed at screening asymptomatic and mildly symptomatic health care workers. Using P-BEST, we were able to screen 1115 patients using a total of 144 clinical diagnostic kits, highlighting the efficiency of our method. Because of stringent lockdowns imposed in Israel, infection rates in April 2020 dropped substantially, and the number of new cases per day continued to decline. We were, therefore, not surprised that no positive carrier was identified within the 1115 subjects screened thus far. The correct identification of a blindly spiked positive sample was used to verify that the pooling, RNA extraction, and PCR processes were properly performed. We note that this would be one typical scenario where the use of P-BEST would be most suitable, i.e., carrier screening when infection rates are very low (<1%), thus providing significant savings in reagents and other diagnostic testing resources and significantly increasing testing capacity.

We are currently exploring several improvements of our method as follows: (i) P-BEST requires the use of widely available automated liquid-dispensing robots. While our current application of P-BEST required ~5 hours for pool assembly using a single-channel robot, we have now successfully tested a more advanced liquid-handling system with a multidispenser arm that can perform this task in about 1 hour. Our method can be easily adapted to work on any standard liquid-dispensing robot, and we are in the process of developing code for these robots from several leading vendors. (ii) To allow P-BEST to be entirely performed in a biosafety level 2 (BSL-2) laboratory, we are currently experimenting with two inactivation methods: directly depositing swab samples into tubes with lysis buffer instead of viral transfer media or heating samples at 70°C for 30 min. (iii) Compressed sensing detection in P-BEST can, in principle, estimate the viral load [C(t) values] of each positive carrier. These estimates may prove clinically relevant and are straightforward in P-BEST. (iv) Because of the global shortage of RNA extraction kits, we are currently testing heat-based and detergent-based methods RNA extraction protocols (24) . While these protocols will be performed in a BSL-3 laboratory, they will reduce the test time by about 2 hours and will also reduce test costs by about 50%. In addition, (v) a recent report has suggested that saliva is more sensitive than nasopharyngeal swabs for SARS-CoV-2 detection (25) . Using saliva samples, which are selfcollected, can greatly increase sample collection capacity. We are, therefore, also optimizing our method for pooling saliva samples.

In summary, here, we presented a novel method for efficient SARS-CoV-2 diagnostic testing that can provide significant increases in both testing efficiency and testing resources and can be readily implemented in clinical diagnostic laboratories. Code and protocols required for implementation of P-BEST can be found at https://github.com/NoamShental/PBEST.

The mathematical field of group testing (26) aims to tackle the problem of efficiently identifying individuals carrying a certain rare trait within a large population by pooling samples and testing each pool as if it were a single sample. In general, pooling is designed in such a way that each individual has a unique "footprint" within the set of pools, thus allowing carrier identification. Group testing dates back to the mid-20th century (27) , and since then, many intricate pooling designs have been described and rigorously analyzed. Group testing has been successfully applied in data compression (28) , computation in the data stream model (29) , and in molecular biology (30, 31) . In our previous studies, we described a combination of group testing and next-generation sequencing for detecting carriers of rare genetic mutations. We detected all individual carriers of rare mutations of a set of 1024 mutagenized Sorghum bicolor plants using a set of 48 pools (11, 12) .

The efficiency of group testing, often measured by the ratio between the number of screened individuals and the number of pools, generally increases with the decreasing frequency of the observed trait. For example, screening for a trait that appears in 0.1% of the population can be done more efficiently than screening for a trait that appears in 1% of the population. Moreover, when the carrier rate exceeds ~8%, group testing is no longer effective, since the required number of pools would be comparable to the number of samples tested.

To optimize efficiency, the pooling design needs to be tailored to the expected carrier rate, by adjusting the number pools and the number of samples per pool. If the true carrier rate exceeds the expected rate, then the method will identify larger sets of suspected carriers, which may include false positives, without any additional false negatives. Therefore, it is imperative to evaluate the robustness of a specific pooling design to higher carrier rates (e.g., Fig. 2 ). In case that the true carrier rate is much lower than the rate for which the pooling design was tailored, testing efficiency would be suboptimal, since a smaller number of pools would have sufficed to correctly detect all positive carriers.

In our current proof-of-concept study of P-BEST, we developed a pooling scheme designed to correctly identify all positive carriers for carrier rates <1.3%. Specifically, we pooled sets of 384 patient samples into 48 pools, each containing 48 samples. Each sample was added to six different pools. Pools were designed on the basis of a Reed-Solomon error correcting code (32) , which, as in our previous work, proved to be robust to experimental noise, e.g., pools that fail to be amplified.

PCR samples with C(t) values of <40 were considered positive. Carrier detection was performed using the gradient projection for sparse reconstruction algorithm (33) as in our former studies (11, 12) . The transformation from fractional to discrete results was done using the following algorithm: The 20 samples with highest scores were selected, and only subsets of these 20 were further considered. In total, 2 20 subsets of samples were tested. Each subset corresponds to a vector x of length 384, in which the entries of the selected samples were equal to 1, and all others were set to zero. Nonzero entries of the product of Mx, where M is the pooling matrix, were replaced by the value 1 and compared to the (binary) measurement vector y. The vector x* for which ‖Mx* − y‖ 1 achieved its minimum was selected.

In each simulation, a number of carriers (ranging from one to five) were randomly selected, thus corresponding to a vector x of length 384, in which the selected carriers were equal to 1, and all others were set to zero. The measurement vector y representing the PCR measurements over the P-BEST pools was provided by binarizing M′x, where M′ is a noisy instance of the pooling matrix M. More specifically, to simulate liquid dispensing volume errors, each nonzero entry in M was sampled from a normal distribution with a mean of 1 and SD of 0.01. A second type of experimental noise, corresponding to dropped pools due to PCR failure, was simulated by randomly deleting entries in y (and corresponding rows in M). The detection algorithm presented in the former section was used to identify carriers using the vector y and the matrix M as input, and its detected carriers were compared to x to evaluate performance.

Naso-and oropharynx swabs were collected for analysis by the laboratory of clinical virology in SUMC, which is approved by the Israeli Ministry of Health to test for SARS-CoV-2 infections. The laboratory uses a clinically approved 2019-nCoV detection kit (Seegene, CA, USA) for both viral nucleic acid extraction and quantitative reverse transcription PCR (qRT-PCR)-based amplification. The kit identifies three SARS-CoV-2 genes: E, RdRP, and N genes. RNA extraction was performed using the STARMag 2019-nCoV kit (Seegene, CA, USA) on a liquid-dispensing robot (STARlet Hamilton, USA). All samples were analyzed individually, and positive and negative results were recorded by the SUMC diagnostic laboratory before our study. To inactivate the virus and extract viral RNA, 500 l of the transfer medium containing the swab is combined with 350 l of lysis buffer of the STARMag kit in a BSL-3 laboratory; following 20′ of incubation, 350 l was used for nucleic acid extraction into a volume of 100 l of extracted genome, from which 8 l was taken for the 2019-nCoV PCR assay.

For initial validation of P-BEST, we used leftover clinical samples from patients who tested positive and patients who tested negative from the clinical virology laboratory of SUMC. Four sets of 384 samples each containing one to two positive samples (sets 1 to 4, respectively) were created. We then conducted a clinical study to screen SUMC health care workers for SASR-CoV-2 infections. The study recruited adult subjects that self-reported as asymptomatic or mildly symptomatic (cough, runny nose, myalgia, or sore throat) with no fever, shortness of breath, or pneumonia. After signing an informed consent, samples were obtained by swabbing the nasopharynx and oropharyngeal cavity each with a different swab using standard techniques. Three batches of 384 participants were collected. As a positive control, one collection batch included a sample from a patient who tested positive via an individual PCR-based diagnostic method. This study was approved by SUMC ethical review board (Helsinki committee).

Pools were prepared using a liquid-handling robot (Arise EZMate-601) using a code written in Python. The code automatically generates a command file for the robot to use. Samples were manually pipetted into 96-well plates from which the robot assembled a set of 48 pools, each containing 48 distinct samples. We generated 48 pools for each sample set. Each pool contained equal volumes from 48 samples (11 l per sample, 528 l per pool). Each individual sample was represented in six different pools. The total time for pooling 384 samples into 48 pools was ~5 hours and was performed in a standard BSL-2 laboratory. Analogously to single samples, 350 l from each pool was then used for nucleic acid extraction into a volume of 100 l, using the clinically approved STARMag kit and the STARlet robot (Hamilton, USA). Then, 8 l was used for qRT-PCR to detect the E gene of SARS-CoV-2, based on an in-house method that was clinically validated and used by the SUMC clinical virology laboratory before the introduction of the 2019-nCoV Seegene kit. We used this PCR method because of shortages of the Seegene 2019-nCoV assay kits used by the clinical diagnostic laboratory at SUMC. To verify compatibility of the Seegene kit, we retested some of our positive pools with the Seegene PCR kit. Pools for the clinical study were generated, as above, but 21 l per sample was used to obtain a total volume of 1008 l in each pool. Testing of the samples collected in the clinical study was performed using the clinically approved Seegene assay kits.

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We thank J. Racah and S. Levy-Tzedek for discussions and manuscript editing. N.S. thanks D. Shental and M. Sobel for discussions. Funding: Funding for this study was provided by a donation to BGU from the Rosetrees Trust. A.P. was supported by an Israeli Science Foundation grant ISF-2484/19 ( 06953-P0029A, filed 13 April 2020). The other authors declare that they have no competing interests. Data and materials availability: All data needed to evaluate the conclusions in the paper are present in the paper, the Supplementary Materials, and/or the P-BEST Github page. Additional data related to this paper may be requested from the authors.