key: cord-0800166-3oij2gd7 authors: Moniuszko-Malinowska, Anna; Czupryna, Piotr; Boczkowska-Radziwon, Barbara; Wasiluk, Tomasz; Borawski, Karol; Dunaj, Justyna; Bujno, Magdalena; Radziwon, Piotr; Rutkowski, Krzysztof; Pancewicz, Sławomir title: A 63-Year-Old Woman with SARS-CoV-2 Infection, Who Developed Severe COVID-19 Pneumonia and Was Supported with Convalescent Plasma Therapy date: 2020-09-29 journal: Am J Case Rep DOI: 10.12659/ajcr.927662 sha: d12ffca31cfca5aad23533aca22ffc4866427c2b doc_id: 800166 cord_uid: 3oij2gd7 Patient: Female, 63-year-old Final Diagnosis: COVID-19 Symptoms: Cough • fever Medication: — Clinical Procedure: PCR • plasma exchange • serology Specialty: Infectious Diseases OBJECTIVE: Unusual clinical course BACKGROUND: There is no evidence-based treatment for coronavirus disease 2019 (COVID-19). We report the case of a 63-year-old woman with SARS-CoV-2 infection who developed severe COVID-19 pneumonia and was treated with convalescent plasma. CASE REPORT: A 63-year-old woman who presented with severe and prolonged course of COVID-19 disease (fever up to 39.4°C, persistent cough, and dyspnea) received a convalescent plasma transfusion, which led to complete recovery. The diagnosis was confirmed by RT-PCR testing using the CFX96 Real-Time System (Bio-Rad, USA) from nasopharyngeal swabs. In laboratory tests, an increase in acute-phase parameters was observed. Chest computed tomography (CT) showed abnormalities typical for COVID-19. On days 9 and 11 of the disease, she received the convalescent plasma prepared from a single plasmapheresis donation from a male donor. This male donor was qualified as a convalescent plasma donor according to Polish guidelines, which are compliant with European guidelines. He donated plasma at the Regional Centre for Transfusion Medicine in Białystok, Poland. The therapy with convalescent plasma led to clinical improvement and normalization of inflammatory parameters. CONCLUSIONS: This report presents a case of severe COVID-19 pneumonia in a 63-year-old woman who was given supportive treatment with convalescent plasma. Ongoing clinical trials will determine whether convalescent plasma therapy is an effective treatment for SARS-CoV-2 infection. The virus that causes coronavirus disease 2019 (COVID-19) is a beta coronavirus related to Severe Acute Respiratory Syndrome coronavirus (SARS-CoV-2). The new virus originated in China and since December 2019 it has spread throughout the world, causing over 21.8 million cases and 773 000 deaths, and the pandemic is still ongoing [1]. SARS-CoV-2 is transmitted by airborne droplets during coughing or sneezing and by close contact with infected individuals. The expression of angiotensin-converting enzyme-2 (ACE-2) receptor in the upper-respiratory tract correlates with the course of virus infection. SARS-CoV-2 utilizes ACE-2 as a receptor to enter host pneumocytes, which may explain its high infectivity. Moreover, comorbidities, such as diabetes, hypertension, heart diseases, and obesity, influence the course of COVID-19 [2] . The diagnosis of SARS-CoV-2 infections relies on PCR in samples taken from the upper-or lower-respiratory tract. The detection of antibodies against SARS-CoV-2 in blood may be useful. In symptomatic cases, chest CT plays a crucial role, as it reveals abnormalities, such as ground-glass lesions, patchy consolidations, alveolar exudates, and interlobular involvement, eventually indicating deterioration. To date, no curative treatment has been found. There are no specific antiviral agents that target the novel virus, but other treatment methods, including plasma obtained from COVID-19 convalescents, are under investigation [3] . We report the case of a patient treated with plasma obtained from a convalescent patient, which led to a complete recovery. A 63-year-old woman with type 2 diabetes and hypertension, without any other risk factors, was referred to the Department of Infectious Diseases and Neuroinfections, Medical University of Białystok, Poland due to fever and cough. Her husband and daughter had been diagnosed with COVID-19. The sample taken from the patient in the Outpatient Department was positive for SARS-CoV-2. The diagnosis was made by reverse transcription-polymerase chain reaction (RT-PCR) testing using the CFX96 Real-Time System (Bio-Rad, USA) from nasopharyngeal swabs. Detection was made with the Seegene (CE-IVD) diagnostic kit. Three regions were detected: RdRP, N, and E. There were no significant abnormalities on physical examination. Chest computed tomography (CT) on day 2 of admission revealed lesions suggestive of COVID-19 pneumonia ( Figure 1A , 1B). Despite 9 days of treatment with oral chloroquine (2×250 mg), the patient remained pyrexial up to 39.4°C and had persistent cough and dyspnea. There was an increase in acute-phase parameters (Table 1) . A chest CT scan performed on day 8 showed progression of inflammatory changes ( Figure 1C , 1D). On days 9 and 11, she was given 250 mL of convalescent plasma intravenously, both prepared from a single plasmapheresis donation from a male donor. The donor had mild COVID-19 symptoms (1-day fever and loss of taste and smell). He was presumed healthy after 2 negative PCR test results. He was a suitable convalescent plasma donor according to Polish and European guidelines [4] and donated plasma at the Regional Centre The patient responded well to plasma therapy, with fever resolution, clinical improvement, and normalization of inflammatory parameters (Table 1) . Subsequent PCR tests were negative 3 times. A change in anti-SARS-CoV-2 IgG antibodies titer was observed. Before infusion, the titer was 81.4 AU/ml (28.05). After the first infusion, the titer was 172 AU/ml (31.05). After the second infusion, the titer was 247 AU/ml (01.06) (DiaSorin, Italy). A repeat CT chest (day 20) showed regression of inflammatory changes ( Figure 1E, 1F) . The patient was discharged on day 23 in good general condition. There were no treatment complications. At follow-up 6 weeks after discharge, she reported no symptoms, and all laboratory parameters were within normal ranges (Table 1 ). The quest for COVID-19 treatment continues. Thus far, there is not enough data to support the effectiveness of any particular There have been very few reports on the usefulness of convalescent plasma transfusions to treat COVID-19. They included a few case series, but no clinical trials. Their outcomes were encouraging, as improvement was observed in all patients treated with convalescent plasma, despite a severe course of the disease [5, 6] . Convalescent plasma can lead to direct neutralization of the virus, modification of an overactive immune system (cytokine storm, Th1/Th17 ratio, complement activation), and a hypercoagulable state [7] . In 2015 Mair-Jenkins et al. reported that convalescent plasma transfusion may be beneficial for patients with viral severe acute respiratory infections. A meta-analysis of 32 studies on SARS coronavirus infections and severe influenza proved that transfusion of convalescent plasma had a clinically relevant impact in reducing the rate of mortality and viral load in these patients [8] . Studies performed during ongoing coronavirus pandemics confirm that patients with a severe infection can benefit from convalescent plasma transfusion and that the risk of adverse effects of such therapy is rather low [4, 6, [9] [10] [11] . The most common adverse reactions are transfusion-related events, involving rigors, fever, anaphylaxis, transfusion-related acute lung injury, circulatory overload, and hemolysis. Therapy seems to be more effective if implemented during first 2 weeks after the onset of symptoms. 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