key: cord-0800011-w8yok8rq
authors: Trikash, Irene; Kasatkina, Ludmila; Lykhmus, Olena; Skok, Maryna
title: Nicotinic acetylcholine receptors regulate clustering, fusion and acidification of the rat brain synaptic vesicles
date: 2020-05-29
journal: Neurochem Int
DOI: 10.1016/j.neuint.2020.104779
sha: 51666299c715b6bbe82f59e978161d0c45b7058d
doc_id: 800011
cord_uid: w8yok8rq

The brain nicotinic acetylcholine receptors (nAChRs) expressed in pre-synaptic nerve terminals regulate neurotransmitter release. However, there is no evidence for the expression of nAChRs in synaptic vesicles, which deliver neurotransmitter to synaptic cleft. The aim of this paper was to investigate the presence of nAChRs in synaptic vesicles purified from the rat brain and to study their possible involvement in vesicles life cycle. According to dynamic light scattering analysis, the antibody against extracellular domain (1-208) of α7 nAChR subunit inhibited synaptic vesicles clustering. Sandwich ELISA with nAChR subunit-specific antibodies demonstrated the presence of α4β2, α7 and α7β2nAChR subtypes in synaptic vesicles and showed that α7 and β2 nAChR subunits are co-localized with synaptic vesicle glycoprotein 2A (SV2A). Pre-incubation with either α7-selective agonist PNU282987 or nicotine did not affect synaptic vesicles clustering but delayed their Ca(2+)-dependent fusion with the plasma membranes. In contrast, nicotine but not PNU282987 stimulated acidification of isolated synaptic vesicles, indicating that α4β2 but not α7-containing nAChRs are involved in regulation of proton influx and neurotransmitter refilling. Treatment of rats with levetiracetam, a specific modulator of SV2A, increased the content of α7 nAChRs in synaptic vesicles accompanied by increased clustering but decreased Ca(2+)-dependent fusion. These data for the first time demonstrate the presence of nAChRs in synaptic vesicles and suggest an active involvement of cholinergic regulation in neurotransmitter release. Synaptic vesicles may be an additional target of nicotine inhaled upon smoking and of α7-specific drugs widely discussed as anti-inflammatory and pro-cognitive tools.

Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels found in many types of both excitable and non-excitable cells. They mediate fast synaptic transmission in neuromuscular junctions and autonomic ganglia, whereas in the brain, they are localized mainly pre-synaptically to regulate neurotransmitter release (Dickinson et al., 2008; Gotti et al., 2009 ).

In addition to plasma membrane, the nAChRs were discovered in the outer membrane of mitochondria to regulate the early events of mitochondria-driven-apoptosis (Gergalova et al., 2012; Skok et al., 2016) . In contrast to muscular and ganglionic nAChRs, which mediate powerful ion currents, those expressed in the brain and mitochondria either mediate small local currents or function metabotropically by engaging the components of various intracellular signaling pathways (Papke, 2014; Skok et al., 2016) .

Structurally, nAChRs are homo-or heteropentamers composed of 10 types of alpha (α1 to α10) and 4 types of beta (β1 to β4) subunits combined in several established combinations; muscular nAChRs also contain γ, δ and ε subunits (Fasoli and Gotti, 2015) . The main nAChR subtypes found in the brain are α4β2 and α7 (Champtiaux and Changeux, 2002) ; α4β2 was shown to be critical for learning and memory (Picciotto et al., 2001) , while α7-containing nAChRs control cell proliferation, survival (Resende and Adhikari, 2009 ) and inflammation (De Jonge and Ulloa, 2007) and are also important for cognition (Leiser et al., 2009; Lykhmus et al., 2020) . The α7 nAChRs were initially discovered as homopentamers (Lindstrom, 1996) ; however, heteropentamers containing β2 subunits were further revealed in the brain (Moretti et al., 2014) , autonomic ganglia (Cuevas et al., 2000) and mitochondria (Skok et al., 2016) .

Synaptic vesicles (SVs) function in presynapse to store and deliver neurotransmitter to the synaptic cleft. During their life cycle, SVs pass through several stages of maturation like de novo biogenesis, acidification, accumulation of neurotransmitter and fusion with pre-synaptic plasma membrane (PM). Consequently, they contain plenty of specific proteins involved in SVs interaction with each other and with PM, neurotransmitter acquisition and release, etc. (Takemori et al., 2006) . Although it is well established that the nAChRs expressed in pre-synaptic nerve terminals regulate the transmitter release (Dickinson et al., 2008) , no direct evidence for the presence of nAChRs in SVs can be found in the literature. The early paper of Jacob, Lindstrom and Berg (1986) , who studied the intracellular pool of nAChRs, demonstrated the nAChRspecific immunoreactivity in coated pits, coated vesicles, multivesicular bodies, and smoothmembraned vacuoles. Another paper shows that nicotine enhanced dopamine refilling and release from striatal synaptosomes, suggesting the involvement of two discrete nAChR subtypes (Turner, 2004) . The aim of the present paper was to investigate the presence of nAChRs in SVs isolated from the rat brain and to study their possible involvement in vesicles life cycle.

All reagents were purchased from Sigma-Aldrich (Saint Louis, USA), unless specially indicated. Acridine orange (AO), R18 (octadecyl rhodamine B chloride) were from Molecular Probe (Eugene, OR, USA.). Octaethyleneglycol-dodecyl ether (C12E8) was purchased from Calbiochem (USA). All chemicals used in the research were of analytical grade. Dilutions were made by deionized water.

Antibodies against α7(1-208) (Lykhmus et al., 2010), α3(181-192), α4(181-192), α7(179-190) (Skok et al., 1999) , α9(11-23) (Koval et al., 2011), β2(190-200) or β4(190-200) (Koval et al., 2004) nAChR fragments were obtained, characterized and biotinylated previously in our lab.

Neutravidin-peroxidase conjugate and SV2A-specific antibody (Invitrogen) were purchased from ALT Ukraine Ltd (representative of Thermo Fisher Scientific in Ukraine).

We used Wistar rats (6-month-old, males, 480 -500 g) and C57Bl/6 mice, either wild-type or α7-/-(Orr-Urtreger et al., 1997) (2-3 months old females, 20-25 g). All animals were kept in the animal facility of Palladin Institute of Biochemistry at the 12 h light cycle. They were housed in a quiet, temperature-controlled room (22-23°C) and were provided with water and dry food pellets ad libitum. Before removing the brain rats were anesthetized with isoflurane, while mice were sacrificed by cervical dislocation. All animal experiments complied with the ARRIVE guidelines and were carried out in accordance with the EU Directive 2010/63/EU for animal experiments. All procedures conformed to the guidelines of the Animal Care and Use Committee (IACUC) of Palladin Institute of Biochemistry, Kyiv. All efforts were made to minimize animal suffering and to reduce the number of animals used.

For Lev treatment protocol, rats were randomly distributed into two groups, 6 animals in each. Experimental group obtained Lev (60 mg/kg) per os once a day (Kasatkina et al., 2018) .

Control group received equivalent volume (100 μl) of vehicle (distilled water). Animals were sacrificed on day 15 of the experiment.

Rat brains were weighed, cut into pieces, transferred to ice-cold 0.32 M sucrose, 10 mM Tris-HCL, pH 7.5 (9 ml/g of brain tissue) and gently homogenized using a glass homogenizer with a Teflon plunger (0.25 mm clearance). All manipulations were performed at 0ᵒC. Synaptosomes were prepared by differential centrifugation of rat brain homogenate (De Lorenzo and Freedman, 1978) . The resultant pellet (crude synaptosomes) was used to obtain PMs and SVs as described earlier (Trikash and Kolchinskaya, 2006) .

The resultant pellet of synaptosomes was lysed with 1 mM EGTA, 10 mM Tris-HCl pH 8.1 at 4°C for 60 min and centrifuged at 20000 g for 30 min. The pellet was used to separate the PMs, while the supernatant was centrifuged at 55000g, 60 min (pellet withdrawn) and again at 130000 g, at 4°C, 60 min. The last centrifugation yielded SVs in the pellet and cytosolic synaptosomal proteins in the supernatant. The resulting SVs pellet was resuspended in 10 mMTris-HCl pH 7.5. The SVs fraction was characterized in detail by electron microscopy and laser correlation spectroscopy previously (Trikash et al., 2004) .

For isolation of synaptic PMs, the pellet obtained after initial synaptosomes fractionation was resuspended in 0.32 M sucrose, 10 mMTris-HCl, pH 7.5 and layered on a discontinuous (0.8 -1.1 M) sucrose gradient. Centrifugation was carried out in the bucket-rotor at 50000 g for 120 min. Membrane fraction was collected at the interface between 0.8 M and 1.1 M sucrose and 4 volumes of 10 mMTris-HCl, pH 7.5 was added. This suspension was centrifuged at 130000 g for 10 min. Finally, the pellet was resuspended in 0.32 M sucrose, 10 mM Tris-HCl, pH 7.5. Purified PMs were used for the membrane fusion and ELISA studies.

Mitochondria were isolated from the brain homogenate of either rats or mice by differential centrifugation according to standard published procedures (Sottocasa et al., 1967; Gergalova et al., 2012) . Their purity was characterized by ELISA using the antibodies against voltage-dependent anion channel (VDAC, mitochondria marker) as described (Uspenska et al., 2018) . Mitochondria of the wild-type and α7-/mice were isolated in Kyiv, while those of β2-/mice were obtained in the Institut Pasteur in Paris in the course of previous collaborative studies.

To prepare detergent lysates, the pellets of PMs, SVs or mitochondria were frozen at -20 o C, thawed and treated with lysing buffer (0.01 M Tris-HCl, pH 8.0; 0.14 NaCl; 0.025% NaN 3 ; 1% Tween-20 and protease inhibitors cocktail) for 2 h on ice upon intensive stirring. The resulting lysates were pelleted by centrifugation (20 min at 20,000g). The protein concentration was established with the BCA Protein Assay kit (Thermo Scientific, Rockford, USA).

To determine the level of various nAChR subunits within the brain PMs, SVs or mitochondria preparations, the immunoplates (Nunc, Maxisorp) were coated with rabbit α7(1-208)-specific antibody (20 µg/ml), blocked with 1% BSA, and the detergent lysates of the membranes, SVs or mitochondria were applied into the wells (1 µg of protein per 0.05 ml per well) for 2h at 37 o C. The plates were washed with water and the second biotinylated α3(181-192)-, α4(181-192)-, α7(179-190)-, α9(11-23)-, β2(190-200)or β4(190-200)-specific antibody was applied for additional 2 h. All antibodies have been preliminary titrated in corresponding antigenic peptides and the doses (dilutions) have been selected according to titration curves: 1:200 for α3-specific, 1:80 for α4-specific, 1:300 for α7-specific, 1:250 for α9-specific, 1:250 for α10-specific, 1:500 for β2-specific and 1:200 for β4-specific, assuming that the initial concentration of all antibodies was 2 mg/ml.

To evaluate the presence of α4β2 and α7β2 combinations, the plates were coated with α4(181-192)or α7(179-190)-specific antibodies (20 µg/ml), mitochondria and SVs preparations were applied as described above and the bound β2 subunits were revealed with β2(190-200)specific antibody (1:500).

To test the presence of nAChR complexes with SV2A protein, the plates were coated with SV2A-specific antibody (10 µg/ml) and the bound nAChRs from SVs preparation were 

The hydrodynamic diameter of the particles in SVs suspension was measured using Malvern 4700 Zetasizer-3 spectrometer (Malvern Instruments, Worcestershire, U.K.) equipped with helium-neon laser "LG-111" (25 mW; wavelength 632.8 nm). Vesicle suspension (50 µl, 50 µg) was injected into cuvette containing 950 μl of either the lysis buffer (1 mM EGTA and 10 mM Tris-HCl, pH 8.1) or solution of synaptosomal cytosolic proteins (1 mg/ml). DLS measurements were carried out at a room temperature (22-24°C). The scattering was detected at the angle of 90°. Data were analyzed by Contin algorithm (Malvern Instruments), which calculates the Z-average size and polydispersity. The transforms of the intensity distribution to volume distributions were calculated using Malvern Instruments software.

The R18 assay is based on the changes of fluorescence intensity upon fusion of R18-labeled SVs and unlabeled PMs. SVs were labelled with R18 (20 µM) as described earlier (Kasatkina et al., 2018) and pre-incubated with vehicle, 75 nM nicotine or 30 nM PNU282987 for 10 min at 37ºC. These SVs (3 µl) were mixed with unlabeled PMs (1:9, based on protein concentration) in the medium of cytosolic synaptic proteins and supplemented with 10 -5 M Ca 2+ (start point, Trikash et al., 2010) . R18 fluorescence was recorded during 4 min thereafter by spectrofluorime-ter Hitachi 650-10S (Japan) at excitation and emission wavelength of 560 and 580 nm respectively with the 5 nm slit width and 530 nm cut-off filter in the emission beam. At the end of each experiment octaethyleneglycol-dodecyl ether (final concentration of 0.1%) was added to the reaction mixture to yield maximal fluorescence dequenching.

Fusion was registered as an increase of R18 fluorescence after lipid bilayer intermixing. The rate of fusion was proportional to the percentage of fluorescence dequenching (%FD):

Where I o is the initial fluorescence signal of R18-labeled SVs, I is the signal measured during the experiment and I d is the maximum value of the dequenched fluorophore signal after addition of octaethyleneglycol-dodecyl ether.

The changes in the SVs acidic state were registered with pH-sensitive fluorescent dye acridine orange (AO), which is accumulated in intracellular compartments proportionally to their pH values. Acidification of SVs results in decrease of AO green fluorescence. 

Dynamic light scattering of SVs preparations demonstrated the presence of two peaks corresponding to particles of about 40 and 500 nm diameters both in the buffer and in the presence of cytosolic synaptic proteins (Fig. 1A-D) . The size of the smaller peak corresponded to reported SVs size (Mundigl and De Camilli, 1994) . Cytosolic proteins are known to promote the SVs clustering by bringing them into close proximity, where they become stably bound or docked (Rottman, 1994; Trikash and Kolchinskaya, 2006; Trikash et al., 2008; Kasatkina et al., 2020 ). When SVs were tested in buffer, the peak of single SVs prevailed (Fig.1A ) and the peak of larger size particles (SV clusters) obviously increased in the presence of cytosolic proteins (Fig. 1C) . Addition of α7(1-208)-specific antibody to the incubation medium resulted in complete disappearance of SVs clusters found in buffer (Fig.1B) and in obvious decrease of clusters number in favor of single SVs when cytosolic proteins were present in the incubation medium (Fig. 1D , summarized in Fig.1E ). These data indicated that α7(1-208)-specific antibody prevents (in buffer) or inhibits (in protein medium) SVs clusters formation suggesting the nAChRs involvement.

The antibody elicited against the large extracellular domain (1-208) of α7 subunit potentially recognizes almost all nAChR subunits due to substantial homology of their extracellular portions. To determine the subunit composition of nAChRs within the SVs preparation we performed Sandwich ELISA, where the brain SVs, mitochondria or plasma membrane preparations were captured with α7(1-208)-specific antibody and were revealed with nAChR subunit-specific antibodies. Such an approach was successfully used by us previously to determine the nAChR subunits content in the brain , B lymphocytes (Koval et al., 2011) and mitochondria preparations . As shown in Fig.2A , synaptic vesicles demonstrated positive signals for α3, α4, α7, α9, β2 and β4 nAChR subunits.

Provided similar protein quantity was applied, the SVs nAChR composition was closer to the brain mitochondria than to brain PMs, the main subunits being α4, α7 and β2.

The α4 subunits form an established combination with β2 subunits in the brain, while α7 subunits can form either homopentamers or heteromers with β2 subunits (Lindstrom, 1996; Khiroug et al., 2002) . To study which combinations are found in SVs, we performed another Sandwich ELISA, where the preparation was captured with α4or α7-specific antibody and revealed with β2-specific antibody. As shown in Fig.2B , a strong signal for α4β2 combination was found in both SVs and mitochondria. The signal for α7β2 combination was much weaker compared to α4β2 and was significantly higher in SVs compared to mitochondria. Since the total signals for α4, α7 and β2 subunits in SVs were quite similar and high ( Fig.2A) , the data obtained with α4/β2 and α7/β2 antibody pairs suggested that only a part of α7 subunits found in SVs form heteromeric α7β2 nAChRs. The specificity of antibodies towards α7 or β2 nAChR subunits was confirmed in the assay using mitochondria of the wild-type, α7-/or β2-/mice. As shown in Fig.   2C , mitochondria of the wild-type mice gave positive signals in both α7 and α7β2 assays, α7-/mitochondria were negative in both assays, while β2-/mitochondria were positive for α7 but not α7β2.

We further performed Sandwich ELISA where the preparation was captured by the antibody against a synaptic vesicle protein SV2A (Feany, 1992) and revealed with nAChR subunit-specific antibodies. Positive signals were found mainly for α7and β2-specific antibodies suggesting the co-localization of SV2A with α7 and β2 nAChR subunits on SVs (Fig.2D ).

Based on the results of Sandwich ELISA experiments, further studies were performed using an α7-selective agonist PNU282987 (30 nM) and nicotine (75 nM), mostly specific for α4β2 nAChRs (Wonnacott, 2014) . We applied them in either dynamic light scattering analysis or in R18 assay, which measure SVs clustering or fusion, respectively. It was found that SVs preincubation with either PNU282987 or nicotine did not affect SV clustering (Fig. 3A) but delayed their Ca 2+ -dependent fusion with the PM by 47.5% and 58.8%, respectively (Fig. 3B) . In contrast, 1 µM nicotine but not 30 nM PNU282987 stimulated acidification of isolated SVs, as measured by the fluorescence of pH-sensitive dye acridine orange (Fig.4) indicating that α4β2 but not α7-containing nAChRs are involved in regulation of proton influx in SVs.

SV2A was identified as a specific binding site for levetiracetam (Lev), a second generation antiepileptic drug (Cortes-Altamirano et al., 2016) . Taking into account the established colocalization of SV2A with α7 and β2 nAChR subunits (Fig.2D) , we studied the effect of Lev treatment on SVs properties and nAChR composition. As shown in Fig. 5 , treatment of rats with Lev for two weeks increased their SVs clustering ( Fig.5A ) but decreased Ca 2+ -dependent fusion with the PM (Fig. 5B ) compared to SVs of non-treated rats. Sandwich ELISA demonstrated that Lev treatment decreased α4 and β2 nAChR subunits in both synaptic PMs and SVs, while α7 subunit was found decreased in PMs and increased in SVs (Fig.6A-B) . Therefore, Lev treatment resulted in redistribution of homomeric α7 nAChRs in favor of SVs. In addition, significant increase was found for α9 subunit in SVs, whereas β4 subunits were up-regulated in the PMs.

Functional nAChRs were traditionally attributed exclusively to cell PM; the intracellular pool was considered as non-mature nAChRs on the way of biosynthesis. The discovery of mitochondrial nAChRs showed for the first time that these receptors can function in intracellular environment (Gergalova et al., 2012) . The data presented here reveal one more intracellular compartment where nAChRs seem to play a significant role: they demonstrate the presence of nAChRs in SVs and their functional role in sequential steps of SV release.

The nAChRs can penetrate into SVs either from the endosomes in the course of SVs biogenesis, or from the PM in the course of SVs recycling (Bonanomi et al., 2006) . The Lev treatment affected the PM and vesicular nAChRs in different ways that is in favor of independent ways of their trafficking. However, an obvious re-distribution of α7 nAChRs between SVs and PMs may be a sign of selective re-uptake of this nAChR subtype from the membrane in the course of SVs recycling. The antibody specific to the nAChR extracellular domain (1-208) influenced SVs clusters formation suggesting that the nAChRs are located on the vesicle surface facing an extravesicular environment. The immediate effect of nicotine on SVs acidification and fast effects of nicotine and PNU282987 on SVs fusion are also in favor of an outward localization of nAChRs on SVs surface. The mechanisms of the nAChR trafficking to and incorporation into SVs need further examination.

The similarity of SVs nAChR content to mitochondrial and not to PM one might be a sign of independent trafficking of newly synthesized nAChRs to either the PM or intracellular compartments. However, the nAChR subunits content was normalized to similar quantity of protein loaded in ELISA. Both SVs and mitochondria preparations contain internal proteins, in addition to membrane ones, therefore, the relation of nAChRs to general protein content is lower compared to purified plasma membranes. Also, we cannot exclude that the density of nAChRs in mitochondria and SVs is lower compared to plasma membranes, which contain highly concentrated nAChRs in synaptic areas.

The presence of α4β2 and α7(β2) nAChRs in SVs and their changes upon Lev administration suggested the importance of cholinergic regulation for SVs functions. Here we studied three processes related to SVs life cycle and functioning: acidification (proton pumps activity as the driving force to refill vesicles with the neurotransmitter), clusters formation and Ca 2+ -dependent fusion with the PM resulting in exocytosis and neurotransmitter release.

The cell-free system, which simulates SVs interaction with each other and/or with the plasma membrane during the exocytotic process in presynapse, allowed us to investigate which steps of this process are affected by Lev treatment or nAChR agonists. The increase of vesicular ΔpH upon nicotine application suggests that the storage capacity of SVs can be positively regulated by cytosolic nicotine through activation of vesicular nAChRs. At least this is true for the vesicular transport of monoamines, acetylcholine, GABA and glycine where ΔpH is a main or prevailing driving force for accumulation.

Up-regulation of α7 (and α9) nAChRs in SVs of Lev-treated rats was accompanied by increased SVs clustering but decreased fusion with the PM. This is in accord with already reported Lev effect on α7 nAChR signaling (Tsudaka et al., 2015) . Accordingly, anti-α7(1-208) antibody prevented SVs clusters formation, while PNU282987 and nicotine delayed SVs fusion.

Taken together, this data suggest that the nAChRs expressed in SVs favor their clustering but prevent fusion.

Nicotine and PNU282987 prevented SVs fusion with the plasma membranes but did not influence SVs clustering. Therefore, the nAChR ion channel activity negatively regulates fusion but is not critical for intra-vesicles interaction. However, SVs clustering was impaired by α7(1-208)-specific antibody and the changes in SVs nAChR content in Lev-treated rats were accompanied by increased SVs clustering suggesting that the nAChRs may be involved in ionindependent manner, possibly, due to their connection to SV2A.

The discovery of functional nAChRs in SVs naturally puts a question about their physiological significance and the ligands, which affect them inside the cell. The α7-containing nAChRs can be activated by choline readily available intracellularly (Alkondon and Albuquerque, 2006) . It was recently shown that α9-containing nAChRs can be activated by phosphocholine and phosphocholine-modified macromolecules (Richter et al., 2016) . Our unpublished data allow suggesting that phosphocholine also affects α7 nAChRs. Recently published evidence demonstrates that long-chain unsaturated acylcholines can be viewed as endogenous modulators of α7 nAChRs (Akimov et al., 2020) These choline derivatives are natural components of membrane lipids, which, similarly to α7 nAChRs agonists, can affect SVs fusion with the PM and the process of neurotransmitter release.

In contrast to fusion, which was affected similarly by nicotine and PNU282987 activating both α4β2 and α7(β2) nAChRs, SVs acidification was affected by nicotine only suggesting that It is well established that the brain nAChRs are located mainly pre-synaptically to regulate the release of several neurotransmitters (Dickinson et al., 2008) . Application of nicotine to striatal nerve terminals (synaptosomes) promoted a sustained efflux of dopamine by affecting two different nAChR subtypes (Turner, 2004) . Our data for the first time show the presence of nAChRs in synaptic vesicles (previously considered as passive transport vehicles) and suggest a new way of cholinergic regulation of neurotransmitter release. According to this suggestion, synaptic vesicles may be an additional target of nicotine inhaled upon smoking and of α7specific drugs widely discussed as anti-inflammatory and pro-cognitive tools. A potential connection of α7 nAChRs and SV2A and a significant increase of α9 and α7 nAChRs in SVs of Lev-treated rats suggest an important role of nAChRs in regulating pro-epileptic activity and an additional mechanism of Lev anti-epileptic effect. SV2A, a specific target of Lev, is prevailing SV2 isoform in most GABAergic neurons and in granule cells of dentate gyrus (Bajjalieh et al., 1994) , which controls hippocampal excitability. SV2 directly interacts and co-traffics with calcium sensor protein synaptotagmin and plays a major role in regulating the amount of this protein in SVs (Yao et al., 2010) . In light of recent publications about the involvement of nAChRs in COVID-19 pathogenesis (Changeux et al., 2020) , the finding of nAChRs in SVs can explain the neurological effects of SARS-Cov2, in particular, seizures provoked by SARS-CoV-2 infection in patients with epilepsy (Vollono et al., 2020) . 

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Fusion of synaptic vesicles and plasma membrane in the presence of synaptosomal soluble proteins

Docking and fusion of synaptic vesicles in cell-free model system of exocytosis

The fusion of isolated synaptic vesicles as a model of final step of exocytosis

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Cotrafficking of SV2 and synaptotagmin at the synapse

Focal status epilepticus as unique clinical feature of COVID-19: A case 1. The α4β2 and α7 nAChRs are present in rat brain synaptic vesicles close to SV2A protein

The α7(1-208)-specific antibody inhibits synaptic vesicles clustering

Activation of either α4β2 or α7 nAChRs delays synaptic vesicles fusion with plasma membranes

Activating α4β2 nAChRs stimulates the proton influx in synaptic vesicles

Levetiracetam up-regulates α7 nAChRs in synaptic vesicles, favors clustering but decreases fusion

We are grateful to Dr Yu. Chunikhin for the help in dynamic light scattering studies.