key: cord-0797938-kd1irkic
authors: Ok, Fesih; Erdogan, Omer; Durmus, Emrullah; Carkci, Serkan; Canik, Aggul
title: Predictive values of blood urea nitrogen/creatinine ratio and other routine blood parameters on disease severity and survival of COVID‐19 patients
date: 2020-07-22
journal: J Med Virol
DOI: 10.1002/jmv.26300
sha: b3c0b4301660876a9a41946cc6b4aa200aad046e
doc_id: 797938
cord_uid: kd1irkic

We aimed to examine independent predictive factors for the severity and survival of COVID‐19 disease, from routine blood parameters, especially the blood urea nitrogen (BUN)/creatinine (Cr) ratio. A total of 139 patients with COVID‐19 were investigated at Siirt State Hospital. According to the disease severity, the patients were categorized as three groups (moderate: 85, severe: 54, and critical: 20). Then, patients were divided into two groups: nonsevere (moderate) and severe (severe and critical). Demographic, clinical data, and routine blood parameters were analyzed. In multivariate model adjusted for potential confounders BUN/Cr ratio (odds ratio [OR] = 1.70; 95% confidence interval [CI]: 1.20‐2.40; P = .002) and neutrophil to lymphocyte ratio (NLR) (OR = 2.21; 95% CI: 1.20‐4.30; P < .001) were independent predictive factors for disease severity. In multivariate Cox proportional hazard model BUN/Cr ratio (hazard ratio [HR] = 1.02; 95% CI: 1.01‐1.05; P = .030), and NLR (HR = 1.17; 95% CI: 1.06‐1.30; P = .020) were independent predictors for survival of COVID‐19 disease. The optimal thresholds of the BUN/Cr ratio at 33.5 and 51.7 had the superior possibility for severe disease and mortality, area under the curve (AUC) were 0.98 and 0.95, respectively. The optimal thresholds of NLR at 3.27 and 5.72 had a superior possibility for severe disease and mortality, AUC were 0.87 and 0.85, respectively. BUN/Cr and NLR are independent predictors for COVID‐19 patient severity and survival. Routine evaluation of BUN/Cr and NLR can help identify high‐risk cases with COVID‐19.

In the latter part of 2019, unknown pneumonia cases began to appear in Wuhan, China (Hubei province). 1, 2 In January 2020, a new beta-coronavirus subtype in China was found through highthroughput sequencing along with the use of throat swab samples. 3 The World Health Organization (WHO) named this coronavirus disease as "COVID-19" and declared it to be a serious international pandemic and public health problem. 4 According to the WHO data, as of early May 2020, over 3 million global cases had been confirmed, more than 20 000 deaths. 5 While this virus affects many systems in animals, it usually occurs in the form of pneumonia in humans. 6, 7 However, mildly-and moderately-affected patients have a better prognosis, and milder symptoms. Yet, the prognosis for severe and critical patients is poor with high mortality rates. 8 In these critical groups, mortality from acute respiratory distress syndrome and multiple organ failure can be rapidly observed.

Therefore, it is vital to predicting the factors that can determine this group of patients at the time of diagnosis.

Blood urea nitrogen (BUN) and creatinine (Cr) are the end products of nitrogen metabolism in humans. Since they are small molecules, they can be easily filtered from the nephrons. Usually, about 30% to 40% of BUN is reabsorbed from tubules, while Cr is not reabsorbed very well. 9, 10 Studies show that the affected neurohormonal system is responsible for the reabsorption process in patients with acute heart failure (AHF). 9 Other studies have similarly demonstrated that the BUN/Cr ratio is more valuable than BUN or Cr alone in predicting the progression of patients with AHF. [11] [12] [13] We believe that multisystem inflammation, including a cytokine storm, can occur in severe and critical groups of COVID-19 patients; this can in turn increase BUN reabsorption and the BUN/Cr ratio by similar mechanisms. As such, this ratio would be beneficial in assessing the severity and survival of those with COVID-19 disease. In addition, there are parameters in peripheral blood whose predictive properties for COVID-19 have been demonstrated in previous studies. The neutrophil-lymphocyte ratio (NLR) and C-reactive protein (CRP) make up some of these parameters. 5, 14 In our study, we aimed to evaluate the role of applicable and cost-effective BUN/Cr ratios, as well as other routine blood parameters, to predict both the severity and survival of those with COVID-19 disease. 15 According to this program, patients divided into three groups as moderate, severe, and critical. Then, patients were classified as nonsevere (moderate) and severe (severe and critical).

Nonsevere patients met all of the features such as the history of contact, respiratory symptoms or fever, typical viral pneumonia involvement in computed tomography, and positive test result of RT-PCR for SARS-CoV-2 RNA. Severe patients also had at least one of the following features; PaO 2 /FiO 2 ≤300 mm Hg, or oxygen saturation (at rest) less than 93%, or shortness of breath, respiratory rate ≥30 times/minute. Respiratory samples were taken and assessed for SARS-CoV-2, according to WHO recommendations for quantitative reverse transcriptase-polymerase chain reaction. A positive test result confirmed the diagnosis of COVID-19 disease from samples. Patients with chronic kidney disease, patients received chemotherapy in the last 6 months, hematological disorders or blood transfusion during hospitalization, age under 18 years old, died on admission, being pregnant, and having missing baseline data were excluded. Those with asthma and chronic obstructive pulmonary disease ere also excluded because they affect the risk groups.

The electronic hospital information system was used for epidemiological data, including demographic, clinical, and laboratory findings. Complete blood count, CRP, and blood chemistry on admission were recorded as laboratory tests. Peripheral venous disease severity. The Cox proportional hazard models analyzed predictors of mortality. In the multivariate-adjusted models, age, gender, hypertension (HT), and heart disease were included. Receiver operator characteristics (ROC) curve analysis was used to determine optimal thresholds via area under the curve (AUC). Youden's Index in ROC curves was used to determine an optimum cut-off value of associated parameters for predicting COVID-19 disease severity and survival.

The Kaplan-Meier analysis was used to evaluate the survival rates of predictive factors' cut-off groups. P value of less than .05 was defined as statistical significance. Table 1 . There were 85 (61.2%) patients in the nonsevere group and 54 (38.8%, 34 severe, and 20 critical) patients in the severe group. The mean age was 47.2 ± 15.7 years in the nonsevere group and 68.3 ± 14.9 years in the severe group (P < .001). There was no significant difference between the two groups in terms of gender (P = .976). The rates of total comorbidities, HT, and heart disease were significantly higher in the severe group (all P < .001).

There was no significant difference in terms of diabetes mellitus (DM) between the two groups (P = .218). The overall number of in-hospital death was 13 (9.4%), and all of them was in severe group (P < .001).

There were many differences in the routine blood parameters between patients in nonsevere and severe groups on admission. 3.2 | Analysis of BUN/Cr ratio and other routine blood parameters for predicting COVID-19 disease severity and optimum cut-off values have milder symptoms and better prognoses than severe or critical patients who typically experience more complex symptoms and have a high mortality rate. 8 Determining the predictive variables for severe disease using routine blood tests can assist in the treatment and management of COVID-19. The demographic, comorbidity, and usual blood parameters of 139 patients with COVID-19 in Siirt, Turkey, were analyzed to determine potential biomarkers for disease severity and survival.

The age distribution of COVID-19 is variable. In our data, we showed that severe patients had an older age. The female-male proportions were similar in both groups. Total comorbidities, HT, and heart disease rates were higher in severe patients. Although the DM rate of severe patients was higher, the difference was not significant.

The clinical features of our patient groups are compatible with other studies. 3, 17 Yuwei et al reported that their total mortality rate was 13.47%. 18 In our cohort, the overall mortality rate was 9.4%. NEUs release reactive oxygen species, causing DNA damage in virus cells. 20 NEUs also interact with different compartments to produce a large number of cytokines and mediators, especially vascular endothelial growth factor. 21 However, the human immune response induced by a viral infection is primarily associated with lymphocytes.

Systematic inflammation significantly reduces CD4+ T lymphocytes and increases suppressive CD8+ T lymphocytes by significantly reducing cellular immunity. 22 In this study, the NLR similarly correlated with disease severity.

Using the multivariate logistic regression model, which adjusted age, gender, and comorbidities to minimize the potential impact of a confounding NLR, it was determined that the NLR was an independent predictor of disease severity (OR = 2.21). In the adjusted Cox proportional hazard analysis, the NLR (HR = 1.17) was associated with mortality.

BUN and Cr levels and the BUN/Cr ratio are the main parameters showing kidney function. 24 The BUN/Cr ratio plays a vital role in the treatment and clinical follow-up of patients with acute myocardial infarction (AMI), with a strong correlation between a high BUN/Cr ratio and the long-term mortality of AMI patients. 25 Yoichi et al reported that the BUN/Cr ratio is associated with an increased risk of death in AHF patients. 11 Gotsman et al 26 indicated that in patients with AHF in their study, the BUN/Cr ratio on admission was associated with increased 1 year and long-term (mean: 6.5-year follow-up) mortality. Brisco et al 27 also found a significant relationship between a high admission BUN/Cr ratio and increased mortality.

The new coronavirus enters the cells using angiotensin-converting enzyme 2 (ACE2) as a receptor. 28 According to the latest human tissue RNA sequencing data, ACE2 is expressed approximately 100 times higher in the kidneys than in the lungs. 29 Coronavirus can affect kidney function by entering kidney cells in a direct ACE2dependent way 30 This study had some limitations. First, this was a retrospective, single-center clinical trial and, therefore, had a small sample size.

Second, even though attempts were made to adjust for many confounders, other unknown features may have played a role. Third, there were no data from the group of mild patients who were sent home at the time of admission. Fourth, there was insufficient data regarding factors such as a high protein diet, which may have influenced the baseline BUN/Cr ratio. Due to these limitations, multicenter comprehensive investigations are needed.

The severity of COVID-19 varies from patient to patient. Advanced age, comorbidity, and immune status are the main risk factors affecting disease severity. Severe illness causes poor survival results.

The BUN/Cr ratio and NLR may be associated with disease severity, and routine use of these parameters may be beneficial in the evaluation of the disease. This study supports the authors' hypothesis F I G U R E 4 Kaplan-Meier survival curves of NLR for the survival of COVİD-19 disease. NLR, neutrophil to lymphocyte ratio that a high BUN/Cr ratio and NLR are independent predictors of COVID-19 patient severity and survival.

We would like to thank all healthcare professionals and hospital management who worked intensively during the pandemic process.

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Predictive values of blood urea nitrogen/creatinine ratio and other routine blood parameters on disease severity and survival of COVID-19 patients

The authors declare that there are no conflict of interests.

FO and OE developed the idea for and designed the study and had full access to all data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. ED and SC contributed to the writing of the report. FO contributed to the critical revision of the report. FO, AC, OE, and SC contributed to the statistical analysis. All authors contributed to data acquisition, data analysis, or data interpretation, and reviewed and approved the final version.

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Institutional Research Committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. This study was approved by the Institutional Ethics Board of Siirt University (No. 2020/05.02).

http://orcid.org/0000-0002-8785-9867Omer Erdogan https://orcid.org/0000-0001-8788-8302Emrullah Durmus https://orcid.org/0000-0001-5021-8495