key: cord-0792442-kwws81ne
authors: Steinhauer, Katrin; Meister, Toni Luise; Todt, Daniel; Krawczyk, Adalbert; Paßvogel, Lars; Becker, Britta; Paulmann, Dajana; Bischoff, Birte; Pfaender, Stephanie; Brill, Florian H.H.; Steinmann, Eike
title: Comparison of the in vitro-efficacy of different mouthwash solutions targeting SARS-CoV-2 based on the European Standard EN 14476
date: 2021-02-11
journal: J Hosp Infect
DOI: 10.1016/j.jhin.2021.01.031
sha: c24f17b6b8946c949f5ed24c852665b8a452d74d
doc_id: 792442
cord_uid: kwws81ne

The outbreak of the SARS-Cov-2 pandemic is triggering a global health emergency alert. Until vaccination becomes available, a bundle of effective preventive measures is desperately needed. Recent research is indicating the relevance of aerosols in the spread of SARS-CoV-2. Thus, in this study commercially available antiseptic mouthwashes based on the actives chlorhexidine digluconate (CHX) and octenidine dihydrochloride (OCT) were investigated regarding their efficacy against SARS-CoV-2 using the European Standard 14476. Based on the requirement of EN 14476 in which reduction of at least four decimal logarithms (log(10)) of viral titer is requested to state efficacy, the OCT-based formulation was found to be effective within a contact time of only 15 sec against SARS-CoV-2. Based on this in vitro-data the OCT-mouthwash thus constitutes an interesting candidate for future clinical studies to prove its effectiveness in a potential prevention of SARS-CoV-2 transmission by aerosols.

to the similarity in appearance to a solar corona [1] . Until the SARS-CoV outbreak in 51 2002, coronaviruses were thought to only cause mild self-limiting infections in 52 humans but were known to cause a wide variety of infections in animals [1] . 17 years 

An overview of Their Replication 186 and Pathogenesis

A novel coronavirus from patients with 188 pneumonia in China

Emerging coronaviruses: genome structure, 190 replication, and pathogenesis

Efficacy of preprocedural mouthrinses 192 in the reduction of microorganisms in aerosol: a systematic review

Particle sizes of infectious aerosols: implications for infection 195 control

Use of mouthwashes against 198 COVID-19 in dentistry

European Standard EN 14476: 201 Chemical disinfectants and antiseptics -Quantitative suspension test for the 202 evaluation of virucidal activity in the medical area -Test method and 203 requirements (Phase 2 / Step 1)

CoV-2 to UV irradiation

Virucidal efficacy of different oral 211 rinses against severe acute respiratory coronavirus 2