key: cord-0790060-xdfou1t8
authors: Lou, Zhiyong; Xu, Yanhui; Xiang, Kehui; Su, Nan; Qin, Lan; Li, Xu; Gao, George F.; Bartlam, Mark; Rao, Zihe
title: Crystal structures of Nipah and Hendra virus fusion core proteins
date: 2006-09-15
journal: FEBS J
DOI: 10.1111/j.1742-4658.2006.05459.x
sha: d5275ce83c67dbb432aae3705b193fa47638928e
doc_id: 790060
cord_uid: xdfou1t8

The Nipah and Hendra viruses are highly pathogenic paramyxoviruses that recently emerged from flying foxes to cause serious disease outbreaks in humans and livestock in Australia, Malaysia, Singapore and Bangladesh. Their unique genetic constitution, high virulence and wide host range set them apart from other paramyxoviruses. These characteristics have led to their classification into the new genus Henpavirus within the family Paramyxoviridae and to their designation as Biosafety Level 4 pathogens. The fusion protein, an enveloped glycoprotein essential for viral entry, belongs to the family of class I fusion proteins and is characterized by the presence of two heptad repeat (HR) regions, HR1 and HR2. These two regions associate to form a fusion‐active hairpin conformation that juxtaposes the viral and cellular membranes to facilitate membrane fusion and enable subsequent viral entry. The Hendra and Nipah virus fusion core proteins were crystallized and their structures determined to 2.2 Å resolution. The Nipah and Hendra fusion core structures are six‐helix bundles with three HR2 helices packed against the hydrophobic grooves on the surface of a central coiled coil formed by three parallel HR1 helices in an oblique antiparallel manner. Because of the high level of conservation in core regions, it is proposed that the Nipah and Hendra virus fusion cores can provide a model for membrane fusion in all paramyxoviruses. The relatively deep grooves on the surface of the central coiled coil represent a good target site for drug discovery strategies aimed at inhibiting viral entry by blocking hairpin formation.

The Nipah and Hendra viruses are highly pathogenic paramyxoviruses that recently emerged from flying foxes to cause serious disease outbreaks in humans and livestock in Australia, Malaysia, Singapore and Bangladesh. Their unique genetic constitution, high virulence and wide host range set them apart from other paramyxoviruses. These characteristics have led to their classification into the new genus Henpavirus within the family Paramyxoviridae and to their designation as Biosafety Level 4 pathogens. The fusion protein, an enveloped glycoprotein essential for viral entry, belongs to the family of class I fusion proteins and is characterized by the presence of two heptad repeat (HR) regions, HR1 and HR2. These two regions associate to form a fusion-active hairpin conformation that juxtaposes the viral and cellular membranes to facilitate membrane fusion and enable subsequent viral entry. The Hendra and Nipah virus fusion core proteins were crystallized and their structures determined to 2.2 Å resolution. The Nipah and Hendra fusion core structures are six-helix bundles with three HR2 helices packed against the hydrophobic grooves on the surface of a central coiled coil formed by three parallel HR1 helices in an oblique antiparallel manner. Because of the high level of conservation in core regions, it is proposed that the Nipah and Hendra virus fusion cores can provide a model for membrane fusion in all paramyxoviruses. The relatively deep grooves on the surface of the central coiled coil represent a good target site for drug discovery strategies aimed at inhibiting viral entry by blocking hairpin formation.

and their ability to jump species barriers have attracted detailed attention, as they have many of the physical attributes to serve as potential agents of bioterrorism [5, 6, 9] .

Paramyxoviruses are enveloped negative-stranded RNA viruses, forming a large family (Paramyxoviridae) divided into two subfamilies with five established and two newly defined genera (Rubulavirus, Respirovirus, Morbillivirus, Pneumovirus, Metapneumovirus and the new Avulavirus and Henipavirus) [6, 10, 11] . Like other paramyxoviruses, NiV and HeV consist of two surface glycoproteins on the viral surface, termed the fusion (F) protein and glycoprotein (G protein, also called attachment protein) [7, 8, 12, 13] . These two glycoproteins are both responsible for viral fusion and entry into host cells [12, 13] . The G protein initiates viral infection by binding to the cellular receptor (attachment), whereas the F protein mediates the subsequent virus-cell membrane fusion process [12] [13] [14] . The F protein undergoes a series of conformational changes in the attachment and subsequent fusion process mediated by the paramyxoviruses [14] [15] [16] .

The F proteins of paramyxoviruses share several features with other viral glycoproteins responsible for membrane fusion, including the hemagglutinin (HA) protein in influenza virus, gp160 of HIV-1, GP of Ebola virus and the spike protein of severe acute respiratory syndrome virus and other coronaviruses. These glycoproteins play a crucial role in the conformational changes during the virus-mediated membrane fusion process [15] [16] [17] [18] [19] [20] . They are all initially synthesized as a single-chain precursor, termed F0 in paramyxovirus, which is then cleaved into two subunits (F1 and F2 in paramyxovirus) by a furin-like enzyme derived from the host cell [7] . F1 and F2 are covalently linked by a disulfide bond, and the complex forms a trimer on the virus envelope. The fusion peptide at the N-terminus of F1 is highly hydrophobic and is considered to be responsible for direct insertion of the F protein into the cellular lipid bilayer [15, 16, 22] . The highly conserved heptad repeat (HR) regions in F1, HR1 and HR2, seemingly act as scaffolding modules. HR1 and HR2 will interact with each other to form a so-called 'trimer of hairpins', 'six-helix bundle' or 'fusion core' in the membrane fusion process. In the fusion core structure, three HR1 helices form a central trimeric coiled coil surrounded by three HR2 helices in an oblique antiparallel manner [15, 16, 23] . This hairpin formation aligns the transmembrane domain in the viral membrane closely with the fusion peptide inserted into the cellular membrane, thus facilitating membrane fusion.

There are at least three different conformations in the membrane fusion process in the model for the viral fusion mechanism proposed from the gp41 structure of HIV. The first is the native (nonfusogenic) conformation in which the HR1 peptides and HR2 peptides are inaccessible. The subsequent conformation is the prehairpin intermediate, in which the HR1 peptides are exposed with their fusion peptides inserted into the target cellular membrane. The last conformation is the fusogenic state, in which the HR1 and HR2 peptides come together and form a highly stable coiled coil aligning the viral and cellular membranes in juxtaposition, facilitating membrane fusion and viral entry [24] . Introduction of exogenous soluble HR1 or HR2 into the virus infection system will block the formation of this hairpin structure and thus inhibit viral fusion and subsequent infection by competing with the endogenous HR1-HR2 interaction and holding the F protein in the intermediate state [16, 25] . Recent studies have shown that the HR2 peptide of NiV and HeV has strong inhibitory activity for membrane fusion in the in vitro cell fusion system of the viruses [26] . Our previous biochemical and biophysical studies have also shown that the complex of HR1 and HR2 in NiV or HeV forms a typical thermostable six-helix bundle [21] . However, detailed structures of the complex and the interaction between HR1 and HR2 in NiV or HeV have not been reported to date.

In this study, a single chain combining the HR1 and HR2 peptides (termed the two-helix) was constructed for both NiV and HeV and expressed in the Escherichia coli system used previously for other paramyxoviruses [27] [28] [29] [30] [31] [32] [33] . We have determined the crystal structures of the NiV two-helix and HeV two-helix to 2.2 Å resolution, thus confirming the formation of a six-helix bundle. These structures also show the typical characteristics of NiV and HeV F proteins as members of the Paramyxoviridae family, and provide a structural basis to explain the inhibitory effects of HR2 on viral fusion and formation of the fusion core structure. The results also show that the HR2 proteins of NiV and HeV are functionally and structurally interchangeable, and this correlates with the sequence similarity of the HR peptides in NiV and HeV (predicted HR1 regions are identical for NiV and HeV but there is a two amino acid difference in the HR2 regions).

The HR1 and HR2 regions of the NiV and HeV F proteins consist of residues 137-178 and residues 453-485, respectively, and were predicted by a computer program called learncoil-vmf [34] . The two peptides encompassing the N-terminal and C-terminal HRs of the NiV ⁄ HeV F protein assemble into a stable trimer of heterodimers [21] . The two-helix molecules were prepared as a single chain by linking HR1 and HR2 with a linker (Fig. 1A) . The NiV two-helix forms crystals with unit cell parameters a ¼ 31

, and belongs to the space group P1. The crystals contain three two-helix molecules (one stable trimer) per asymmetric unit and diffract to 2.2 Å . The solvent content is estimated to be 21% with a Matthews coefficient (V m ) of 1.5 Å 3 AEDa )1 . The HeV selenomethionyl derivative crystals belong to space group P1 with unit cell parameters a ¼ 32

and diffraction extends to 2.2 Å . Assuming the presence of three two-helix molecules (one stable trimer) per asymmetric unit, the solvent content is estimated to be 26%, with a Matthews coefficient (V m ) of 1.7 Å 3 AEDa )1 . Selected data statistics are shown in Table 1 .

The HeV two-helix crystal structure was determined by multiple wavelength anomalous dispersion (MAD) from a single selenomethionyl derivative crystal. Three selenium sites were located in one asymmetric unit from Patterson maps calculated with the program cns [35] . The model was improved by cycles of manual building and refinement using the programs o [36] and cns [35] . The structure was subsequently refined to a final R-value of 21.3% and a free R-value of 27.4%.

The NiV two-helix crystal structure was determined by molecular replacement with the HeV two-helix structure as a search model. After rotation and translation function searches with cns [35] , the model was improved by cycles of manual building and refinement using the programs o [36] and cns [35] . The final R-value and the free R-value for the refinement were 22.5% and 28.0%, respectively.

The three-dimensional structures of NiV and HeV two-helix are very similar, with an rmsd of 1.4 Å for all Ca atoms, which correlates with their high amino acid sequence identity. Unless otherwise stated, we will concentrate on the structure of the NiV two-helix in the following discussion. The fusion core of NiV has a rod-shaped structure approximately 50 Å in length and with a maximum diameter of 28 Å . The NiV two-helix complex structure is a six-helix bundle comprising a trimer of NiV two-helix molecules. The center of this bundle consists of a parallel trimeric coiled coil formed by three HR1 helices, against which three HR2 helices pack in an antiparallel manner ( Fig. 2A,B) . The Residues highlighted with a red background are those that are strictly conserved; residues highlighted with a yellow background are residues that are more than 80% conserved. Residues that are important for HR1 and HR2 interactions, including the e and g positions in HR1 and the a and d positions in HR2, are labeled. Residues that are important in the end deep groove in HR1 and HR2 are framed and labeled with a blue triangle and red star. SV5, parainfluenza virus 5 or simian virus 5; NDV, Newcastle disease virus; HRSV, human respiratory syncytial virus. (C) Helix wheel analysis of the predicted coiled-coil regions of NiV F protein HR1 and HR2, which are represented as purple and golden wheels, respectively. The two substitutions in HeV relative to NiV were located in positions g and f of the helix wheel, not in the a or d positions, which are important for the central HR1 trimer formation. The substitutions of RL to KI are also conservative.

N-terminus of HR1 and the C-terminus of HR2 are located at the same end of the six-helix bundle, placing the fusion peptide and transmembrane domains close together. A region of about 270 amino acids would be located at the other end of the six-helix bundle between HR1 and HR2 in the postfusion state of NiV F protein.

The eight amino acids in the linker and several terminal residues were disordered in the electron density map and could not be traced in any of the three molecules. In one asymmetric unit of the NiV structure, the three molecules include residues 143-176 in HR1 and 455-484 in HR2, 143-175 in HR1 and 455-484 in HR2, and 143-175 in HR1 and 458-485 in HR2, respectively. In the HeV structure, the three molecules in one asymmetric unit include residues 143-176 in HR1 and 455-484 in HR2, 143-175 in HR1 and 454-484 in HR2, and 143-177 in HR1 and 457-484 in HR2, respectively. The rmsd of the NiV two-helix and the HeV two-helix is 1.3 Å for all Ca atoms.

Residues 143-176 of HR1 fold into a nine-turn a-helix that extends over the entire length of the coiled coil. As in other naturally occurring coiled coils of the fusion core, the residues in the a and d positions of the fusion core diagram representation [24] of HR1 are predominantly hydrophobic (Fig. 1B) . A sequence alignment of NiV with other representative paramyxovirus fusion proteins shows that the residues in these two HR positions are highly conserved (Fig. 1B) .

Residues 455-484 of HR2 form an eight-turn amphipathic a-helix stretching the entire length of the coiled coil. Each HR2 peptide packs against the long grooves formed by the interface of the three HR1 helices, and no interaction is observed between individual HR2 helices ( Fig. 2A,B) . The C-terminus of HR2 ends with V484, which is aligned with N143 of HR1; N143 is also the N-terminus of the HR1 domain. The N-terminus of HR2 starts with I456, which is aligned with L175 of HR1 (Fig. 2C) . 

Three HR2 helices of the NiV fusion core pack against the outside of the central coiled-coil trimer in an obliquely antiparallel manner, which suggests a common interaction mode for the other well-studied Paramyxoviridae virus fusion proteins. The HR2 helices interact with HR1 mainly through hydrophobic interactions between hydrophobic residues in HR2 and the hydrophobic grooves on the surface of the central coiled coil (Fig. 3A) . The interaction region of HR1 can be divided into three parts: the upper deep groove (I144-V158), the central shallow groove (V159-T164) and the lower deep groove (A165-L172) (Fig. 3B ). Residues M463, I474, L481 and V484 of HR2 are anchored in the deep groove of HR1 and make a significant contribution to the hydrophobic interactions between HR1 and HR2 (Fig. 3B ). Sequence comparison between NiV ⁄ HeV and other paramyxovirus fusion proteins shows that residues contributing to the HR1-HR2 interaction (e and g positions in HR1, a and d positions in HR2) are highly conserved (Figs 1B  and 3B ). This pattern of sequence conservation can also be shown by a helical wheel representation of one HR1 helix and one HR2 helix [21] . Sequence comparison between NiV and SV5 fusion proteins shows that five out of nine changes (including one A to V) occur in the e and g positions of HR1, and six out of nine changes (including two L to I and one I to V) occur in HR2 at the a and d positions. In contrast, only 13 out of 15 nonconservative changes occur at the outside f, b and c positions in HR1, and three out of 21 nonconservative changes occur at positions other than a and d in HR2 (Fig. 1B) .

Among paramyxovirus fusion proteins, only the SV5 and human respiratory syncytial virus (hRSV) fusion core structures have been determined to date [32, 37] . The NiV fusion core structure has a similar conformation to both SV5 F1 and hRSV, and can be superim- posed with an rmsd of 0.68 Å and 0.67 Å between all Ca atoms, respectively. We will focus our structural comparison on NiV F and SV5 F, as the fusion core structures of hRSV and SV5 share significant similarity. Although the NiV and SV5 fusion cores share a very similar topology, they also have some significant differences. First, the structure of the NiV F fusion core HR1 peptide (143-176) is much shorter than its counterpart in the SV5 fusion core (122-185), although HR2 has the almost same length in NiV (455-484) and SV5 (440-477) fusion cores (Fig. 4A,B) . Second, the hydrophobic grooves on the surface of the central coiled coil have some significant differences, especially in the lower deep groove (Fig. 4C,D) . In the structure of the NiV F fusion core, the lower deep groove formed by T164, A165, T168, V169 and L172 is much deeper than the equivalent region of the SV5 fusion core structure, formed by A157, T158, L161, G162 and V165 (Fig. 3E ). This groove is so deep that we even observe that the bottom of the grooves are connected to each other and form a connective hole in the HR1 surface. Residue M463, which occupies the d position in the HR2 region and faces the center of the trimer, anchors into this groove and greatly contributes to the stability of the fusion core complex. Residue L161 in the SV5 structure makes this groove more shallow than its counterpart, T168, in NiV due to the longer hydrophobic side chain. Sequence alignment with other Paramyxoviridae viruses also shows that NiV has the shortest hydrophobic residue in the T168 position and the longest residue in the M463 position.

Although they share many differences from other Paramyxoviridae fusion core proteins, the NiV and HeV F fusion cores also share certain similarities and show high conservation. Among the Paramyxoviridae, the NiV and HeV fusion cores have the shortest structures and sequences. However, all paramyxovirus fusion cores share the same core parts and are highly conserved, both in sequence (Fig. 1B) and in threedimensional structure (Fig. 3B) . These facts suggest that the structure of the NiV F fusion core may share common features with all Paramyxoviridae virus fusion cores, leading us to propose the NiV F fusion core structure as a model for Paramyxoviridae fusion cores. Furthermore, the conserved deep grooves at both ends of the NiV fusion core may provide a structural basis for the design of wide-spectrum therapeutics targeting the Paramyxoviridae family.

Structural studies of the influenza virus HA and HIV gp41 have established a paradigm for understanding the mechanisms of viral and cellular membrane fusion [18] . The similarity between the NiV F protein and other widely studied viral fusion proteins, as well as previous biochemical analysis [38] , indicates a similar mechanism of membrane fusion mediated by the NiV and HeV fusion proteins. The structures of the NiV and HeV fusion cores reported here add to the repertoire of paramyxovirus six-helix bundle fusion core structures, providing greater structural information in order to understand the formation of the fusion-active state of genus Henipavirus. Similar to SV5F and HIV gp41, the NiV and HeV fusion proteins probably undergo a series of conformational changes to become fusion-active. The The end deep groove on the surface of heptad repeat 1 (HR1) in the NiV F fusion core. HR1 is shown as a white molecular surface, and HR2 is represented by gold sticks. (B) The same orientation and position on the surface of HR1 in the SV5 fusion core. HR1 is shown as a white molecular surface, and HR2 is represented by green sticks. The position of the deep groove is highlighted by red lines. (E) Details of residues in the end deep grooves of the NiV F and SV5 fusion cores. NiV F fusion core residues are shown as yellow sticks with black labels; SV5 fusion core residues are shown as green sticks with purple labels.

Nipah ⁄ Hendra virus fusion core structures Z. Lou et al. fusion loop, which inserts into the cellular membrane, is accepted to have the distinct conformational states proposed for the NiV F protein fusion core, including the native state, the prehairpin intermediate, and the fusion-active hairpin state. Several biological and inhibition studies have also provided good evidence that the fusion core in the crystal structure presented here is the final, stable form of the protein, which is the fusion-active state following one or more conformational changes. First, gel filtration and chemical crosslinking results demonstrated that the oligomeric state of the two-helix protein was a trimer. Even at high concentrations of the crosslinker, the monomer ⁄ dimer bands could be observed [31] . Second, NiV and HeV infection in vitro can be potently blocked by peptides corresponding to the C-terminal HR (HR2) of the HeV fusion envelope glycoprotein 39. These features suggest that the NiV F protein also undergoes a conformational change mechanism, similar to influenza HA and HIV gp41.

As membrane fusion is a very important process during virus infection, inhibition studies have been carried out to find effective drugs to block virus infection by targeting the membrane fusion step. In the case of HIV-1, several strategies to block hairpin formation have been successfully developed to identify viral entry inhibitors that bind to the hydrophobic pocket and grooves on the surface of the central coiled coil consisting of HIV-1 gp41 N peptides. These useful viral entry inhibitors include D peptides, five-helix, and synthetic peptides derived from N or C peptides [40] [41] [42] . Successful viral entry inhibitors have also been identified for other viruses, such as T20 for HIV-1 and GP610 for Ebola virus. Analogous strategies could also be used for the design of NiV ⁄ HeV fusion inhibitors. In 2005, several reports showed that NiV ⁄ HeV infection in vitro can be potently blocked by specific HR2 peptides. The improved second-generation HR2 peptides, which use poly(ethylene glycol) to facilitate peptide synthesis and increase solubility, also show good IC50 values in in vitro assays. The applied chemical modifications are also predicted to increase the serum half-life in vivo and should increase the chances of success in the development of an effective antiviral therapy [50] . The well-defined hydrophobic grooves on the surface of the central coiled coil of the NiV F protein fusion core identified from our structure can offer a reasonable explanation for the inhibition of NiV and HeV infection. Furthermore, the structures reported here provide significant targets for the design of NiV and HeV antiviral agents.

Purification and crystallization ) the two-helix constructs of both NiV and HeV Fusion proteins were prepared as a single chain by linking the HR1 and HR2 domains with an eight amino acid linker (GGSGGSGG). The PCR-directed gene was inserted into the pET22b vector (Novagen, Shanghai, China), and the target plasmids were transformed into BL21 (DE3) competent cells. The cells were cultured at 310 K in 2 · YT medium containing 100 lgAEmL )1 ampicillin and were induced with 0.2 mm isopropyl thio-b-d-galactoside (IPTG) when the culture density (D 600 ) reached 0.6-0.8. The selenomethinoine derivative HeV two-helix protein was expressed in M9 medium containing 30 mgAEL )1 selenomethionine in E. coli strain BL21 (DE3). The two products were both purified by nickel-nitrilotriacetic acid affinity chromatography followed by gel filtration chromatography. The purified NiV two-helix and HeV two-helix derivative were dialyzed against crystallization buffer (10 mm Tris ⁄ HCl, pH 8.0, 10 mm NaCl) and concentrated to 10-15 mgAEmL )1 . Initial crystallization conditions were screened using Crystal Screen reagent kits I and II (Hampton Research, Aliso Viejo, CA, USA) and a poly(ethylene glycol) screening kit prepared in-house.

Good-quality NiV two-helix crystals were obtained from 0.1 m Tris ⁄ HCl (pH 8.5) ⁄ 29% poly(ethylene glycol) 4000 (v ⁄ v). Good-quality HeV two-helix derivative crystals were obtained from 0.1 m Hepes (pH 6.5) ⁄ 10% poly(ethylene glycol) 4000 (v ⁄ v). The preparation and crystallization of the two-helix proteins of NiV and HeV have previously been reported in detail [21] .

Data collection from the NiV two-helix crystal was performed in-house on a Rigaku RU200 (Tokyo, Japan) rotating-copper-anode X-ray generator operated at 48 kV and 98 mA (CuKa; k ¼ 1.5418 Å ) with an Mar345 imageplate detector. The crystal was mounted on nylon loops and flash-cooled in a cold nitrogen gas stream at 100 K using an Oxford Cryosystems (Oxford, UK) cold stream and with the reservoir solution as cryoprotectant. Data were indexed and scaled using the HKL2000 programs denzo and scalepack [43] . The HeV two-helix selenomethionine derivative crystal was mounted on nylon loops and flash-frozen in a cold nitrogen gas stream at 100 K using an Oxford Cryosystems cold stream and with 0.1 m Hepes (pH 6.5) ⁄ 25% poly(ethylene glycol) 400 as cryoprotectant. MAD data were collected by a rotation method using a Mar CCD detector with synchrotron radiation beamline 3W1A of the Beijing Synchrotron Radiation Facility. Data were collected from a single selenomethionyl derivative crystal at peak (0.9799 Å ), edge (0.9801 Å ) and remote (0.9500 Å ) wavelengths to 2.2 Å . Data were indexed and scaled using denzo and scalepack programs [43] .

For determination of the HeV two-helix structure, initial MAD phasing steps were performed using solve [44] , and density modification was performed using resolve [45] . The program o [36] was used for manual tracing of the experimental density map, and the initial structure was subsequently refined using the programs o [36] and cns [35] . The NiV two-helix structure was determined by molecular replacement with the HeV two-helix structure as a search model. Rotation and translation function searches were performed with the program cns [35] . The model was further improved by manual building and refinement using the programs o [36] and cns [35] . The quality of the two structures was verified by procheck [46] , with none of the main-chain torsion angles located in disallowed regions of the Ramachandran plot. Structure determination and refinement statistics are summarized in Table 1 . The figures were generated with the programs grasp [47] , pymol [47] and molscript [48] .

Coordinates and structure factors for the NiV and HeV fusion core crystal structures have been deposited in the RCSB PDB with accession numbers 1WP7 and 1WP8, respectively.

Fatal encephalitisdue to Nipah virus among pigfarmers in Malaysia

Nipah virus: a recently emergent deadly paramyxovirus

Emerging infectious diseases. Nipah virus (or a cousin). strikes again

A novel morbillivirus pneumonia of horses and its transmission to humans

Emerging viral diseases of Southeast Asia and the Western Pacific

Emerging Paramyxoviruses

The exceptionally large genome of Hendra virus: support for creation of a new genus within the family Paramyxoviridae

Complete nucleotide sequences of Nipah virus isolates from Malaysia

Nipah virus ) a potential agent of bioterrorism?

Virus Taxonomy: Classification and Nomenclature of Viruses, 7th Report (vanRegenmortel MHV, Fauquet CM

Complete nucleotide sequence of avian paramyxovirus type 6 isolated from ducks

Functional expression and membrane fusion tropism of the envelope glycoproteins of Hendra virus

Functional properties of the fusion and attachment glycoproteins of Nipah virus

Paramyxovirus fusion: a hypothesis for changes

The paramyxovirus fusion protein forms an extremely stable core trimer: structural parallels to influenza virus haemagglutinin and HIV-1 gp41

Mechanisms of viral membrane fusion and its inhibition

Structural basis for membrane fusion by enveloped viruses

Membrane fusion mediated by coiled coils: a hypothesis

Coiled coils in both intracellular vesicle and viral membrane fusion

Nipah ⁄ Hendra virus fusion core structures Z

Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin

Basis for fusion inhibition by peptides: analysis of the heptad repeat regions of the fusion proteins from Nipah and Hendra viruses, newly emergent zoonotic paramyxoviruses

Influenza fusion peptides

A trimeric structural domain of the HIV-1 transmembrane glycoprotein

HIV entry and its inhibition

Enfuvirtide for prophylaxis against HIV infection

Membrane fusion tropism and heterotypic functional activities of the Nipah virus and Hendra virus envelope glycoproteins

The fusion protein core of measles virus forms stable coiled-coil trimer

Biochemical and biophysical analysis of heptad repeat regions from the fusion protein of Menangle virus, a newly emergent paramyxovirus

Design and analysis of postfusion 6-helix bundle of heptad repeat regions from Newcastle disease virus F protein

Crystallization and preliminary X-ray crystallographic analysis of the trimer core from measles virus fusion protein

Six-helix bundle assembly and characterization of heptad repeat regions from the F protein of Newcastle disease virus

Both heptad repeats of human respiratory syncytial virus fusion protein are potent inhibitors of viral fusion

Crystallization and preliminary X-ray diffraction analysis of post-fusion six-helix bundle core structure from Newcastle disease virus F protein

LearnCoil-VMF: computational evidence for coiled-coil-like motifs in many viral membrane-fusion proteins

Crystallography and NMR system: a new software suite for macromolecular structure determination

Improved methods for building protein models in electron density maps and the location of errors in these models

Structural basis for paramyxovirus-mediated membrane fusion

Sixhelix bundle assembly and analysis of the central core of mumps virus fusion protein

Inhibition of Henipavirus fusion and infection by heptad-derived peptides of the Nipah virus fusion glycoprotein

Design of potent inhibitors of HIV-1 entry from the gp41 N-peptide region

Inhibiting HIV-1 entry: discovery of d-peptide inhibitors that target the gp41 coiled-coil pocket

Protein design of an HIV-1 entry inhibitor

Processing of x-ray diffraction data collected in oscillation mode

Automated MAD and MIR structure solution

Maximum-likelihood density modification using pattern recognition of structural motifs

PROCHECK: a program to check the stereochemical quality of protein structures

Protein folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons

MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures

This work was supported by the NSFC (grant number 30221003).