key: cord-0787739-20eq5meh
authors: Esposito, Dominic; Mehalko, Jennifer; Drew, Matthew; Snead, Kelly; Wall, Vanessa; Taylor, Troy; Frank, Peter; Denson, John-Paul; Hong, Min; Gulten, Gulcin; Sadtler, Kaitlyn; Messing, Simon; Gillette, William
title: Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays
date: 2020-06-04
journal: Protein Expr Purif
DOI: 10.1016/j.pep.2020.105686
sha: bd36068997ef748710fc7511a8ceb8f5e4fd15e6
doc_id: 787739
cord_uid: 20eq5meh

The SARS-CoV-2 spike trimer is the primary antigen for several serology assays critical to determining the extent of SARS-CoV-2 exposure in the population. Until stable cell lines are developed to increase the titer of this secreted protein in mammalian cell culture, the low yield of spike protein produced from transient transfection of HEK293 cells will be a limiting factor for these assays. To improve the yield of spike protein and support the high demand for antigens in serology assays, we investigated several recombinant protein expression variables by altering the incubation temperature, harvest time, chromatography strategy, and final protein manipulation. Through this investigation, we developed a simplified and robust purification strategy that consistently yields 5 mg of protein per liter of expression culture for two commonly used forms of the SARS-CoV-2 spike protein. We show that these proteins form well-behaved stable trimers and are consistently functional in serology assays across multiple protein production lots.

The need for high quality protein reagents is an important aspect of the response to the COVID-19 pandemic [1] . Screening for the presence and extent of an immune response will be a critical tool in controlling the spread of the infection until the development of an effective vaccine. Additionally, such monitoring will provide essential data to policy makers as they develop guidelines to limit virus spread in the population. With the ubiquitous presence of related coronaviruses in the population (e.g. SARS-CoV, MERS-CoV, common cold coronaviruses OC43 and HKU1), the tests must be highly specific for SARS CoV-2. Several serology assays, both published and in progress, are employing the S protein (hereafter referred to as spike) in enzyme-linked immunosorbent assays (ELISA). The specificity inherent in the spike protein [1, 2] makes it an obvious target for therapeutic interventions and for use in serology studies to assess the prevalence of immune responses to a specific coronavirus. Two spike antigens are currently widely used: the receptor binding domain (RBD), which interacts with the extracellular ACE2 receptor, and a much larger soluble spike ectodomain modified for stability to mimic the prefusion native spike trimer conformation [2] . While robust production of spike RBD domain was recently reported [3] , high quality soluble spike trimers remain a difficult antigen to both express and purify. Publication of one form of this protein cites a yield of 0.5 mg/l [2] , with another publication suggesting yields as high as 5 mg/l [3] . However, many unpublished reports indicate consistent yields only in the 1-2 mg/l range, providing a significant challenge for large-scale serology assay development. In addition, some of these publications provide limited information on purification details or protein quality assessment, which make interpretation of the yields challenging.

To support an NIH-led serosurvey on the extent of the coronavirus immune response in ~ 10,000 human samples, our lab was tasked to produce RBD and spike proteins for ELISA assay optimization and deployment [4] . We found our RBD production yield to be similar to published reports, however, spike production was problematic with our initial attempts producing inconsistent results and lower yields than those reported in the literature. Without a robust method in place in our lab for determining spike titer in culture supernatants, we were limited to the crude method of SDS-PAGE gel analysis for estimating expression levels. Due to the size of spike (138 kDa), heavy level of glycosylation, and low expression levels, SDS-PAGE gel analysis was inconclusive when comparing multiple lots of expression supernatants. Thus, the work presented here is intended to provide a robust method for those wishing to reliably produce SARS CoV-2 spike protein in quantities sufficient for serology assays, structural biology, or simply to better understand some of the production variables affecting the yield. It is expected that this protocol could be further improved and perhaps eventually replaced by a stable cell line production platform. Nevertheless, the approaches outlined here allowed us to improve the production yield of spike protein significantly by modifying cell culture temperature and harvest time, as well as improving the purification process. The final proteins produced were highly pure, formed appropriate trimeric structures, and were functional as antigens in ELISA assays.

Taken together, these improvements allowed the production of sufficient spike antigen for more than 500 ELISA plates per liter of culture and generated enough protein from a single 4-liter expression to support a robust serosurvey being conducted by the NIH [4] . 

Harvested culture supernatants were clarified by centrifugation (4000 x g, 20 min, 4°C) followed by filtration (catalog# 12993, Pall Corporation, Port Washington, NY). When used in column chromatography, clarified supernatants were concentrated and buffer exchanged by TFF.

Specifically, a MasterFlex peristaltic pump (Vernon Hills, IL) fed the clarified supernatant to a 30 kDa molecular weight cutoff cassette (catalog# CDUF002LT, MilliporeSigma, Burlington, MA). The clarified supernatant was concentrated to 10% of the initial volume and then buffer exchanged with 5 volumes of 1x PBS, pH 7.4 (Buffer A, diluted from 10X PBS, catalog #70011069 Thermo Fisher Scientific, Waltham, MA). Following buffer exchange, the TFF 6 cassette was rinsed with 200-250 ml Buffer A, to collect any protein remaining in the cassette.

Clarified supernatants for use in batch purification were not buffer exchanged.

Chromatography was conducted at room temperature (~22°C) using NGC medium- The sample was desalted into Buffer A using a HiPrep 53-ml 26/10 desalting column (GE Healthcare #17-5087-01, Chicago, IL) with 14-ml injections at 9 ml/min for all steps. The final protein sample was created by combining the bulk elutions from multiple runs of the desalting column. The protein concentration was determined by measuring the A 280 using a Nanodrop One spectrophotometer (Thermo Fisher Scientific, MA, USA). Final protein was dispensed as 0.5-ml or 0.05-ml aliquots, snap frozen in liquid nitrogen, and stored at -80°C. To assess the oligomeric state of the protein, a single 0.5 ml aliquot of the final protein was thawed and analyzed by analytical size-exclusion chromatography using a 10/300 Superdex200 analytical column (GE Healthcare, Chicago, IL), with a flow rate of 0.5 ml/min.

For the batch purification from filtered culture supernatants, 20 ml of Ni-charged MagBeads (Cat #L00295, GeneScript, Piscataway, NJ), previously equilibrated in 1x PBS adjusted to 300 mM final NaCl concentration (Buffer B), were placed on the bottom of 2 x 5-liter Thompson flasks (10 ml per flask) and 2 liters of filtered culture medium was added to each flask. Flasks were shaken at 105 rpm at 27°C for 3 hr. After incubation, the supernatant was decanted into a 4-liter glass beaker, a rare-earth magnet was used to capture the beads to the bottom of the beaker, and the medium was removed and saved as "flow through". Buffer B (150 ml) was added to the beaker to suspend the beads, which were then transferred to 500-ml Corning centrifuge bottles and shaken at ~22°C (room temperature for all subsequent steps) for 5 min. Beads were collected, the wash removed, and a second 150 ml wash step was performed.

The beads were washed an additional two times with 30 ml of Buffer B in a 50-ml Corning tube while shaking for 10 min for each wash (total of 4 wash steps). Proteins were eluted from the washed beads by addition of Buffer B with increasing imidazole concentrations of 25, 150, 300, and 500 mM. 20 ml of appropriate buffer was used for each elution and the beads were shaken in 50-ml Corning tubes for 10 min before collecting. Samples of each elution fraction were analyzed by SDS-PAGE and Coomassie-staining and appropriate fractions were pooled. The pool was treated as above for buffer exchange, final gel analysis, and storage.

Transmission electron microscopy of the purified VRC and Mt. Sinai spike proteins was carried out by dilution of thawed final samples to 0.02 mg/ml in 20 mM Tris-HCl, pH 8.0, 200 mM NaCl followed by loading onto glow-discharged carbon support film grids (CF200-CU, Electron Microscopy Sciences). Grids were washed twice in buffer (20 mM Tris-HCl, pH 8.0, 200 mM NaCl) and stained with 0.75% w/v uranyl formate (pH 4.5) three times using filter paper to blot away the stain prior to immediate application of additional stain. A final staining step was carried out for 30 sec with the stain then removed by wicking with filter paper, and the grids were dried under an incandescent lamp. Stained grids were imaged on a Hitachi 7650 electron microscope at 40,000x magnification.

In order to assess batch-to-batch reproducibility of spike antigens, purified VRC spike proteins from five different purifications were used as antigens in an ELISA with positive control CoV-2 serum at multiple dilutions. The ELISA protocol was carried out as reported [4] using 8 100 ng per well of the various spike proteins and dilutions of 1:500, 1:10,000, and 1:100,000.

Absorbance measurements were made at 450 nm (signal) and 650 nm (background control) using a BMG Labtech PHERAstar plate reader which is capable of linear absorbance readings up to an OD of ~ 4.

To produce SARS-CoV-2 antigens for the development of serology assays, we initially followed standard procedures for secreted protein production: transfection using the manufacturer's protocols, expression at 37°C, harvest at three days post-transfection, tangential flow filtration of the culture supernatant, immobilized metal ion chromatography with linear gradient elution, and size exclusion chromatography. However, this process resulted in very low yields (0.9 and 0.3 mg/l, respectively, for Mt. Sinai and VRC spike as seen in Table 1 ). These yield levels were not high enough to support large serology studies without significant scaleup and associated high costs. The urgent need and the narrow time frame to provide protein support for NIH serology studies limited our ability to perform extensive troubleshooting and optimization. Rather, we assessed several parameters with internally controlled experiments (e.g.

splitting an IMAC pool in two and passing the two aliquots over different size exclusion resins).

This was essential as safety protocols for laboratory personnel during the pandemic limited the number of staff in the lab. Using this approach, we were able to obtain information on the effect of multiple parameters including the temperature of cell culture, harvest time, and mode of target capture. post-harvest production process followed the procedure of TFF > IMAC > desalting column.

Numbers represent yields from independent experiments. nd -not determined.

Inspection of the SDS-PAGE/Coomassie staining analysis of the initial IMAC (not shown), suggested that we could improve the separation of spike from contaminant by adjusting the elution parameters. The relatively high affinity of the His-tagged spike proteins for the column enabled the use of a 6 CV linear elution gradient from 25 mM to 175 mM imidazole which eluted the majority of contaminants before spike was eluted in a step elution of 325 mM imidazole ( Fig. 2A) . Analysis of intermediate purification steps by the low-resolution method of SDS-PAGE/Coomassie staining suggested significant protein loss was occurring during size exclusion chromatography (SEC). This correlated with published methods of spike purification [3] and suggested that high concentrations of spike might lead to protein loss by precipitation.

We found it difficult to quantitate the amount of target loss during these steps, likely due to heavy glycosylation, which is reported to interfere with Bradford analysis of protein concentration [5, 6] . By using a desalting column for buffer exchange, rather than SEC or diafiltration, our modified protocol is designed to minimize protein manipulations that might lead to protein loss especially during post-IMAC steps. Thus, we compared purification yields from parallel experiments with either SEC or a desalting column as the final purification step ( Table 1) . The desalting column protocol led to a consistently higher yield of ~2 mg/l, indicating that this method improved protein recovery. In addition, very little difference in protein quality was observed in these samples, particularly in the case of the VRC spike protein, again arguing against the need for the SEC step. As can be seen in Fig. 2B , the VRC spike protein was consistently of higher purity than the Mt. Sinai spike protein. However, ELISA data comparing these two proteins suggests that these minor impurities have no significant effect on the antigenicity in the assay [4] . Nevertheless, for downstream processes which require higher levels of purity, Mt. Sinai spike may require additional purification steps.

Reducing expression temperature during recombinant protein expression, in both E. coli and the baculovirus/insect expression system, can significantly increase yields of soluble protein [7] . In transient CHO systems, temperature reductions are commonly used to enhance protein production [8] . However, similar modifications in HEK293-based systems are less frequently reported. Previous work in our lab and others suggests one limitation to protein secretion in transiently transfected mammalian cell culture might be the secretion process itself. While multiple factors appear to be involved, a recent paper suggests that limiting components in the secretory pathway appear to be the major bottleneck [9, 10] . We did observe a significant amount of spike protein in the Expi293 cell pellets (data not shown), suggesting that some protein was being held up in the endoplasmic reticulum, or was otherwise failing to completely mature through the secretory pathway. Low cell viabilities (<70%) at harvest were another sign of potential toxicity caused by secretory failures. Thus, we compared the purification yield of VRC and Mt. Sinai spike from transiently transfected cells incubated at either 32°C or 37°C after the addition of enhancers 18-hours post-transfection. As seen in Table 1 and Fig. 2B , the lower temperature expression led to a dramatic increase in yield to ~5 mg/l for both CoV-2 spike proteins we tested. This yield is similar to that cited in a recent report of the Mt. Sinai spike protein produced at 37°C and using a diafiltration approach to buffer exchange [3] . However, unpublished results from other colleagues in the field using this construct suggest 1-2 mg/l is a more consistently observed result, suggesting that these improvements will make a marked enhancement to yield. Published VRC spike protein yields are 0.5 mg/l [2] , suggesting that our modified procedures improved production of this protein by nearly 10-fold.

We also investigated the time of harvest as a variable, as this is a common approach to improve yield from secreted protein platforms [11] . In initial experiments incubated at 37°C, increasing the time of harvest from 72 hours post-transfection to 96 hours resulted in some improvement in yield ( Table 1) . We did not test production at the lower temperature of 32°C at 72 hours, as cell growth is considerably slower at this temperature and we anticipated this would not be enough time for high levels of expression. Therefore, 96 hours was used as the standard for 32°C expression as noted above. However, we did explore longer growth times at the lower temperature and saw no further yield increase for either VRC or Mt. Sinai spike by harvesting 120 hr post-transfection (Table 1, Fig. 2B ). For these reasons, we chose 32°C and 96 hours as our optimal conditions for future expression.

Of note, we have also expressed and purified the analogous recombinant spike proteins from 4 other beta-coronaviruses using the original protocols described here as controls for the serology assay [4] . Interestingly, our yields of the spike proteins from SARS-CoV, MERS-CoV, OC43, and HKU1 (4.6, 10.6, 8.4, and 5.7 mg/l, respectively) in a single experiment from cells incubated at 37°C were considerably higher than that of the CoV-2 spike under similar

conditions. This argues that SARS CoV-2 spike is unique in some aspect of protein stability and/or recombinant protein expression, as these other coronavirus spike proteins were cloned in the same vector and using the same strategy as the VRC CoV-2 spike. We anticipate that the new protocols outlined here might further improve the yield of those proteins as well.

Our observations of protein loss during steps in which spike is either concentrated or exposed to large surface areas, suggested that we might further improve purification by eliminating the TFF, which is necessary to achieve maximum protein capture from the culture supernatant during subsequent column IMAC. Thus, we used magnetized IMAC beads to capture VRC spike in batch mode from filtered lysates. The final protein purified with this approach was similar in terms of quantity and final purity ( Table 1 and Fig. 2C ) to proteins purified by the TFF/IMAC/desalting protocol. This batch process has several distinct advantages over the more complex protocol. First, it eliminates the need for the labor-intensive TFF process, which for large-scale transient cultures can take many hours, and the requirement for specialized equipment and consumables. Second, when combined with a gravity flow approach to buffer exchange, this process completely obviates the need for a protein workstation, making the process accessible to many laboratories without FPLC technology, and making it highly scalable.

We evaluated the functionality of spike proteins by structural and conformation-based methods. Unlike natural SARS-CoV-2 spike proteins, these engineered recombinant forms of the protein utilize an exogenous trimerization domain from phage T4 to form appropriate trimeric structures in the absence of cell membranes. To assess the oligomeric state, our soluble spike proteins were analyzed by electron microscopy and analytical size exclusion chromatography (AnSEC). Negative-stain TEM images of spike particles (Fig. 3A) clearly show the expected trimeric structure and closely resemble previous published images of engineered soluble trimer spike proteins [2] . In addition, as seen in Fig. 3B , recombinant spike proteins eluted during AnSEC at a volume expected for a protein of ~520 kDa rather than that expected for monomeric spike (~180 kDa). This result was consistent over all preparations of both VRC and Mt. Sinai spike proteins, and in no cases did we observe any detectable monomeric spike protein by AnSEC. These macromolecular based approaches support the conclusion that our purified spike proteins adopt tertiary and quaternary structures like those of previously published engineered spike trimers.

An initial lot of VRC spike protein was previously used to develop and optimize a serology assay using an ELISA format [4] . To assess whether spike proteins produced by our modified protocols had equivalent ELISA sensitivity, multiple production lots of VRC spike were compared in the serology assay. Fig. 4 demonstrates that independent batches of VRC spike proteins performed nearly identically in the assay at multiple concentrations of positive control sera. Assay sensitivity was consistent across multiple lots of protein produced with the same expression conditions (duplicate bars of identical color) and also across protein produced at different expression times and temperatures. Consistent lot-to-lot performance of spike protein is an essential part of a high-quality, sensitive serology assay.

In summary, we presented multiple improvements to the production of SARS CoV-2 spike protein that will allow labs with modest protein expression and purification experience to consistently produce high-yield and high-quality protein for use in a variety of applications.

While we optimized the process to generate proteins for serology assays, the high quality of these proteins makes them amenable for biochemical, biophysical, and structural studies, or as substrates for drug screening. We hope that the parameters we highlighted in this report will help others to overcome bottlenecks in spike production and guide future optimization work. 

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