key: cord-0787223-wjmofmol authors: Chaachouay, Noureddine; Douira, Allal; Zidane, Lahcen title: Herbal Medicine Used in the Treatment of Human Diseases in the Rif, Northern Morocco date: 2021-04-07 journal: Arab J Sci Eng DOI: 10.1007/s13369-021-05501-1 sha: d89f5cb54b9e634d8c54f68959d71bbfc8662ad2 doc_id: 787223 cord_uid: wjmofmol Since the beginning of time, the Moroccan people have used many medicinal plants as a popular medicine to cure many human and livestock health problems. Yet, few studies have been carried in the past to properly document and promote traditional ethnomedicinal knowledge. This study was conducted out from July 1st, 2016 to July 30th, 2018 in the Rif; it was aimed to establish the list of medicinal plants, together with the association of ethnomedicinal knowledge. The ethnomedicinal data obtained were from 1000 traditional healers using semi-structured discussions, free listing, and focus groups. Family importance value, plant part value, fidelity level, the relative frequency of citation, and informant consensus factor were applied in data interpretation. Plant species were accumulated, and deposited at the Plant, Animal Productions and agro-industry laboratory, Ibn Tofail University. A total of 280 medicinal plants belong to 204 genera and 70 families were documented. Asteraceae with 29 species was the most used family in this study area. Rosmarinus officinalis L. (RFC = 0.189) was the species the most commonly prescribed by local traditional healers. Similarly, the leaf was the most useful part of the plant (PPV = 0.364), the most frequent affections were osteoarticular affections (ICF = 0.983), and the majority of herbal remedies were prepared from a decoction (38.6%). The results of the present investigation confirmed the presence of indigenous ethnomedicinal information of plant species in the Rif’s area to treat various disorders. More investigation on phytochemical, pharmacological, and toxicological should be considered to determine new drugs from these reported plants. Medicinal plants have been prescribed and used extensively for thousands of years to treat various disorders and ailments in traditional herbal medicine systems all over the world [1] . In all ancient civilizations and on all continents, we find traces of this use. Thus, even today, despite advances in pharmacology, the therapeutic use of plants is very present in some countries, especially in developing countries [2] . The World Health Organization (WHO) estimates that unevenly, 80% of the people from developed and developing nations depend on traditional medicines, especially on plantbased medicine in primary healthcare [3] . The use of plant species for healing purposes is a matter of culture and tradition in Morocco. It should be noted that for the primary health needs, a large portion of Moroccan people utilizes traditional methods of medicine to treat their diseases [3] [4] [5] [6] . The inability of many developing countries to supply contemporary pharmaceutical medications [7] [8] [9] [10] [11] [12] , and the high cost of many drugs, has forced local communities to search for alternative products, such as medicinal plants, that have proven effectiveness and safety and are culturally acceptable. The Rif region is one of the most biologically diverse regions in the Mediterranean, with some of the rarest biogeographical areas in the world and biodiversity of primary importance with many plants of therapeutic interest [13] . For this reason, this region is the source of many medicinal plants marketed throughout Morocco and abroad and the use of plants in herbal medicine is still very present in this region. On the opposite, data on medicinal plants in this region are rare and insufficient. To complete partial and fragmentary studies those have been carried out throughout the Rif [13, 14] , Talassemtane National Park [15] , and Tingitane Peninsula [16] . It is, therefore, necessary to undertake them to identify the local uses of plant species. It is in this context that an ethnopharmacological study was carried out in the Rif, which has a lithological diversity, structural and floristic plants important enough to establish the catalog of medicinal plants used in the traditional treatment of diseases, especially herbal medicine, as an alternative to enhance, preserve and rationally use them. The current study was conducted out in the Tangier-Tetouan-Al Hoceima region (North of Morocco) where the Rif's area was located. It extends between 34° and 36° of latitude in the North and 4° to 6° of longitude in the East. It is bounded in the North by the Strait of Gibraltar and the Mediterranean Sea, in the South by the Rabat-Sale-Kenitra region and Fez-Meknes region, in the East by the Eastern Region, and in the West by the Atlantic Ocean (Fig. 1) . The total geographical area of the Rif is 11,570 km 2 and the population of the city is about 3,549,512 inhabitants with an average population density of 222.2/km 2 [17] . The population is mixed between Arabic and Amazigh ethnicity. The Rif is marked by Mediterranean weather with the highest temperature up to 45 °C during summer (July-August) and below 0 °C during winter (December-January) and the average annual rainfall ranges from 700 to 1300 mm which falls mainly between October and February [18] . It is mountainous with elevations ranging from 145 to 2456 (Jbel Tidirhine) meters above mean sea level and the area dominated by species such as Abies marocana Trab., Pinus halepensis Mill., Cannabis sativa L., Cedrus atlantica (Endl.) Quercus suber L., Quercus ilex L., and Quercus canariensis Willd. Principally families of Rif Fig. 1 The geographical position of the Rif region are very much dependent on subsistence farming, livestock, and to a more secondary space, from forest resources for their livelihood. Ethnopharmacological investigations were carried out from July 2016 to July 2018 to collect information on medicinal plants utilized to treat multiple human sicknesses in the Rif region. In this work, the sample has been developed through a mode of probabilistic sampling random stratified [19, 20] non-proportional, it is divided into 28 strata, having concerned the cities, villages, douars, and souks weekly in the area of study. It is based on environmental factors (climate, soil, and altitude), vegetation, and the distribution of the population. The techniques employed for data collection were semi-structured interviews [21] , open-ended, group discussion, free listing, and noted and recorded with a digital voice recorder. 1000 informants within aged 17-95 were randomly selected for interviews (cautery installer, farmers, elder people, bonesetters, herbalists, and therapists) in Rif (weekly markets, pharmacies, hospitals, houses, and mosques). By conducting a stratified random sampling [22] , samples are then formed in each of the 28 strata, including seven urban communes: [S 1 : Al Hoceim (40), S 12 : Chefchaouen (40), S 15 (39) ] and they are put together to make up the overall sample of 1000 informants. Knowing that the number of people surveyed varies from one stratum to another depending on the abundance of medicinal plants sought (Fig. 2) . The time spent on each interview was approximately 20 min to one hour. The information collected concerning the profile of the interviewee (age, gender, level of study, monthly income, family situation, and locality) and the ethnopharmacological data for each plant include the common local name, the route of administration, the method of preparation, the dosage, the part used, the condition of the plant used and the diseases treated "Appendix A". The people in the Rif region speak Amazigh, Arabic dialects and therefore, interviews were conducted in Amazigh or Arabic dialects. All the documented data were later translated into English. Fertile specimens for the present study were collected in the field (197 plant species), in herbal stores (50 plant species) , and at the homes of traditional healers (33 plant species) in the Rif. The informants were always provided with fresh plant material, either collected with them, by them, or available at their market stands (Fig. 3) . Field observations were also used to record the habitat of each plant species with the assistance of local guides and interviewed informants. Based on ethnopharmacological knowledge provided by our informants, plant specimens with their exact taxonomy were ordered alphabetically by ethnomedicinal uses, vernacular name, and family name. The identification and nomenclature of the collected material vegetal were done first in the field and completed at the Plant, Animal Productions, and Agro-industry Laboratory (Fig. 4) . These plant species mentioned by the informants were taxonomically identified using floristic and taxonomic references, especially "The medicinal plants of the Morocco" [23] , "List of vascular plants of Morocco tomes I and II" [24] , and "Practical vegetation of Morocco" [25] [26] [27] . All voucher specimens have been preserved during documentation and deposited in the Ibn Tofail University, Morocco Herbarium for future reference. Ethnopharmacological data collected are recorded on questionnaire sheets to be analyzed, studied, and confirmed or overturned at the end. Then these data were registered and interpreted by Microsoft Excel 2010 and IBM-SPSS Statistics Base 21. A representative and the quantitative scientific method was applied to examine the socio-demographic data of the informants (ANOVA One-way and Independent Samples T-Test). Further, the recorded data were analyzed by various quantitative indices like family importance value (FIV), the relative frequency of citation (RFC), plant part value (PPV), fidelity level (FL), and informant consensus factor (ICF). , where RU is the number of applications notified of total portions of the medicinal plant and RU plant part is the number of uses reported per part of the medicinal plant. The part among the most important PPV is the most used by the informants. Fidelity level is the rate of interviewees who mentioned the uses of certain medicinal plants to treat a particular disease in the study region. The FL ratio is determined using this equation [31] : where N p is the number of interviewees that require the application of a plant species to cure a special affection and N is the sum of interviewees that use the medicinal species as a drug to treat any given illness. Informant consensus factor (ICF) was determined to investigate an agreement between the interviewees on the related remedies for each group of diseases [32] where N ur is the number of use-reports in each disease category and N t is many species used. The values for the Informant Consensus Factor range from 0 to 1. A total of 1000 respondents were interviewed. Based on socio-demography, these participants were categorized into different classes as given in Table 1 . Among the participants, 52.7% were females and the remaining were males 47.3%, with a sex ratio female/male of 1.11. From the total respondents, 76% were married, 10.8% divorced, 9.2% widowed, and 4% unmarried. In terms of age, the age groups of 40-60 were very high compared to other groups (47.6%). Just 30.6% were more than 60 years old, 21.4% were 20-40 years old, and 0.4% of informants were below 20 years old. Regarding educational status, the majority of respondents (69.1%) were illiterate, while (23%) and (6.7%) respondents attended primary and secondary school, respectively. Only 1.2% of respondents were attended higher education. Considering the income/month, most of the informants were unemployed (41.6%), while (38.6%) of these informants had low income/ month level, (17%) with average level income/month, and (2.8%) with higher-level income/month. Family Importance Value (FIV) The To assess the relative importance of the reported plant species, the relative frequency of citation (RFC) was calculated from the informants' citations. In the present study, the highest value reported was 0.189, and the lowest value was 0.001 for each species as given in Tables 2, 3 The present study showed that 165 medicinal plant species (59%) used by the Moroccan pharmaceutical medicines today are collected from the agriculture (cultivated) (Fig. 5 ), 101 species 36% were collected from wasteland ( Fig. 6 ) (All lands affected by water erosion, wind erosion, floods, waterlogging, soil salinization, and soil alkalization) and only 14 species (5%) were introduced by marketing in other regions. Medicinal plant species have a fundamental unit for use as alternative medicines systems in Morocco and are the basis for the discovery of natural ingredients for the development of therapeutic agents in pharmacology. The study conducted in the Salé region [33] shows that the local population uses some plants to prevent and treat the COVID-19, which was mentioned by the Moroccan Rif community. In Moroccan Rif, the uses of medicinal species have increased during the COVID-19 pandemic as a preventive behavior. Citrus limon (L.) Burm. f. has been used to relieve cough, and as an expectorant in bronchitis. Allium sativum L. Allium cepa L. and Zingiber officinale Roscoe are indicated for respiratory disease (cold and cough), and other symptoms related to influenza. Eucalyptus globulus Labill. is indicated for symptoms of respiratory disease (bronchitis, rhinitis), due to the presence of 1-8-cineol [34] . Indications of respiratory conditions: Foeniculum vulgare Mill. Plantago lanceolata L., Pimpinella anisum L., Silybum marianum L., Laurus nobilis L., Malva sylvestris L., Thymus vulgaris L., and Glycyrrhiza glabra L. are indicated by informants for cough associated with a cold, sore throat, fever laryngitis, and tonsilitis. Hedera helix L. is indicated as antispasmodic, other indications are anti-inflammatory, and in the treatment of flu and fever. Black seeds of Nigella sativa L. are globally known as a spice and as such as a food item. Fidelity level (FL) designates the choice for medicinal plants to be better for other species in the treatment of a particular ailment. The plant species that are extensively used by the indigenous inhabitants have more important FL values than those that are few popular. In this study, the FL ranged from 45.5% to 100% for medicinal plant use age. The study determined 240 medicinal plant species (85.71%) achieve the greatest fidelity level (FL = 100%) and the remaining 40 plant species achieve reasonable FL. The results of the ICF calculation show that the value in our study ranges from 0.944 to 0.983 per uses categories (Table 10) . Results revealed that the very best ICF (0.983) value was obtained for osteoarticular diseases with 867 usereports for 16 plant species. It's followed by dermatological diseases (ICF = 0.981), neurological diseases (ICF = 0.974), genitourinary diseases (ICF = 0.973), metabolic diseases (ICF = 0.972), cardiovascular diseases (ICF = 0.968), digestive system diseases (ICF = 0.945), and respiratory system diseases (ICF = 0.944). Indigenous people in the study area used many methods of preparation. The results showed that the majority of remedies were prepared from decoction (38.6%) and infusion (34%), followed by cataplasm (11.3%) cooked (7.6%) and raw (2.3%). The percentage of the other methods of preparation grouped (maceration, inhalation, fumigation) doesn't exceed 6.2%. The main solvent with the plant was water, but milk, butter, tea, and honey, cereal oils were also widely used ingredients. The route of administration in this study varies with the type of disease treated and the actual sites of the ailments. The result revealed that herbal medicine was administered through different routes. Generally, the majority of informants prepared remedies were applied mostly by oral (82.4%) followed by massage (6.3%), swabbing (5.2%), other modes of administration (3.4%), and rinsing (2.7%). As part of this study, our investigations identified 280 species and subspecies used for medicinal purposes. These medicinal species belong to 204 genera and 70 botanical = 0.165) . Several studies about Rosmarinus officinalis L., with biological, pharmacological, and phytochemical approaches have been conducted and indicate anti-proliferative, anti-inflammatory, anti-bacterial, and anti-oxidant healing properties [41] [42] [43] . As this plant species is widely distributed in almost all Moroccan regions, including the study area, and is easily spread, it is frequently used and a widely available raw material. According to these results, it is recommended that medicinal species having high RFC values should be further screened in pharmacological, toxicological, phytochemical, and biological activities for any novel molecules or chemicals for treating various ailments. Moreover, these species should also be prioritized for conservation as their preferred uses may place their populations under threat due to over-harvesting. Furthermore, the ethnopharmacological plants with greater values of RFC confirm the fact that these plant species were well accepted to the largest of the autochthonous people [44] . Among the 280 medicinal plants, 86 species were used for the treatment of digestive system diseases, whereas 41 species were used to treat respiratory system diseases, 30 species neurological diseases, 29 species cardiovascular diseases, 29 species metabolic diseases, 27 species genitourinary diseases, 22 species dermatological diseases, and 16 species were used to treat osteoarticular diseases. The fidelity level (FL) of each species is also evaluated from the available information. It indicates the informant's choice for each ailment and the potential of the species related to the diseases as well. FL values in this study varied from 45.5% to 100%. The study determined 240 species of plants with an FL of 100%, even without considering plants that were mentioned only once for better accuracy, whereas below FL values are obtained for plant species that are employed for several purposes. This result means that the indigenous people tended to rely on one specific medicinal plant for treating one certain disease than for several illnesses. Consequently, plant species not previously studied and have maximum FL should be recommended for further study related to clinical practice [45] . The diseases treated in the Rif region were classified into 8 ailment categories. The highest ICF values were recorded The plants frequently used to treat these disorders might contain active ingredients and thus were well known by locals. It expresses the best consensus between medicinal plants and treated osteoarticular diseases because informants interviewed used specific plant species commonly for agreement among the informants on the use of these plant species to treat a particular disease category [39] . The Rifain people used many various plant parts for preparing remedies. In our investigation, leaves were the most commonly utilized plant part with PPV = 0.364 application in traditional medicinal remedies, followed by seed (PPV = 0.2263), and whole plant (PPV = 0.097). Many studies conducted elsewhere in other countries also showed the dominance of leaves in the preparation of remedies [1, 2, 4, 37, [49] [50] [51] . The reason why leaves and aerial parts were mostly used could be that they are most easily accessible and their richness in secondary metabolites produced by photosynthesis. From the conservation point of view, the use of leaves is sustainable, since, if the withdrawal of aerial parts is not excessive, will not prevent the development and/or reproduction of the plant [52] . In this context, the use of leaves in herbal preparations implies a more sustainable practice but the harvesting of roots (which oftentimes requires uprooting) may result to plant death [53] . On the other hand, collecting leaves has a less detrimental impact on plants compared to the harvesting of roots and stem barks especially where there are no sustainable harvesting strategies in place [54] . Besides, a collection of leaves would be much easier and sustainable than that of roots or flowers [55] . Concerning the methods of preparation, decoction (38.6%), and infusion (34%). are the most common preparation methods that are used by indigenous people of Rif. The major solvent with the plant was water, but milk, butter, tea, and honey, oils were also extensively used as ingredients. Local people of Rif add honey, sugar, salt, orange peel, banana, or lemon to increase the palatability of a preparation. Moreover, honey is considered sacred to Muslims and occupies an important place in Islamic popular medicine [56, 57] . Indeed, honey is considered an instant energy source and is often used in all parts of the world to improve the acceptability of medicinal plants having a bitter taste unbearable. The decoction is a mostly used method for the preparation of indigenous herbal practices due to its easy preparation by mixing with water or tea [58] . However, the decoction provides assemble the greatest for the active constituents and attenuates or eliminates the toxic effect of some compounds. Decoction and infusion are very valued and often favored by popular healers in Morocco [4, 7, 15, 59, 60] . The predominance of decoction of the different plant species in the Rif is in total agreement with most of the carried out ethnobotanical studies [4, 50, 51, [61] [62] [63] [64] . Generally, the main route of application for herbal therapies was oral (82.4%). Furthermore, the oral mode of administration is a preferred route all over the world [10, 14, 36, 38, 39, 65] . The predominance of oral treatment may be explained by a large incidence of internal illnesses in the Rif region. The administration of oral treatment may be defined by a high degree of internal illnesses in the region [66] . Our investigation revealed that the use of plant species is playing an essential purpose in satisfying the basic healthcare requirements of the indigenous people residing in the Rif, northern Morocco. In this study, an ethnopharmacological catalog constituted of 280 plant species, belonging to 204 genera in 70 families, has been developed, these results of the study showed that there is a great variety of medicinal plants. The number of medicinal plants recorded for the prevention and therapy of human ailments is a good indicator of the potential that exists locally so long as the scientific procedure is added to the indigenous knowledge in terms of traditional herbal medicine. In light of this, medicinal plants with the most important RFC and FL values were identified could be taken up for further phytochemical, pharmacological, and clinical studies that allow the development of new herbal preparations or formulation of novel drugs addressed to improve the quality of life for treating various human diseases. We declare that there is never a conflict of interest with any commercial business about the document. Consent for publication Consent for publication was obtained from participants. The study was authorized by the ethical committee of Ibn Tofail University. Before starting data collection, we obtained oral informed permission in each case on a site level and then individually before each interview. We also informed indigenous people that it was a student academic project and investigation was only for our research purposes, not for any financial or other benefits. Informants provided verbal informed consent to engage in this study; they were free to withdraw their information at any point in time. Those informants have accepted freely the idea and they have consented to have their names and personal data to be published. Author contributions NC carried out field research in the Rif, compiled the literature sources, data analysis, Realization manuscript and evaluation, interpretation, and wrote the manuscript, helped in data, and made a substantial contribution to data analysis. AD performed data analysis and drafted the manuscript. LZ designed the research and identification of plant species. All contributors see and confirm the final paper. Funding This investigation did not receive any particular grant from funding businesses in the public, commercial, or not-for-profit areas. Availability of supporting data All data collected and analyzed in this paper are included in the article and attached in the form of 'Appendices' as additional files. Plant specimens are collected and deposited in Ibn Tofail University, Kenitra, Morocco. Ethnobotanical study of medicinal plants used in Arjan-Parishan protected area in Fars Province of Iran Traditional knowledge of wild medicinal plants used by the inhabitants of Garam Chashma valley, district Chitral World Health Organization: WHO traditional medicine strategy Etude ethnobotanique des plantes médicinales dans la région de Mechraâ Bel Ksiri Ethnobotany study of medicinal plants used in the treatment of respiratory diseases in the middle region of Oum Rbai La flore médicinale traditionnelle de la région de Béni-Mellal (Maroc) Ethnobotanical study of medicinal plants used to treat osteoarticular diseases in the Moroccan Rif Gul, S: Traditional knowledge of medicinal herbs among indigenous communities in Maidan Valley Ethnobotanical studies of potential wild medicinal plants of Ormara An ethnobotanical study of medicinal plants used by tribal and native people of Madhupur forest area Ethnopharmacological assessment of medicinal plants used against livestock infections by the people living around Indus river An Ethnobotanical study of Medicinal Plants in high mountainous region of Chail valley (District Swat-Pakistan) Poisonous medicinal plants used in the popular pharmacopoeia of the Rif, northern Morocco Ethnobotanical and ethnopharmacological studies of medicinal and aromatic plants used in the treatment of metabolic diseases in the Moroccan Rif Étude ethnobotanique des plantes médicinales dans le Parc National de Talassemtane (Rif occidental du Maroc) Evaluation exhaustive de la diversité des plantes aromatiques et médicinales de la Péninsule Tingitane (Maroc) HCP: Haut-commissariat au plan, Monograpie de la région Ethnobotany: principles and applications Ethnobotany: a methods manual Ethnopharmacological survey on antihemorrhagic medicinal plants in South of Benin Recherches floristiques sur le massif montagneux des Béni-Snassène (Maroc oriental) Les plantes médicinales du Maroc, 3ème édition Fennec. Casablanca Moroc Catalogue des plantes vasculaires du Nord du Maroc, incluant des clés d'identification Flore pratique du Maroc, Dicotylédones (pp) Catalogue des plantes vasculaires rares, menacées ou endémiques du Maroc. Herbarium Mediterraneum Panormitanum Flore pratique du Maroc: manuel de détermination des plantes vasculaires. Pteridophyta, Gymnospermae, Angiospermae (Lauraceae-Neuradaceae) M: Ecological importance of Myrtaceae in an eastern Brazilian wet forest Cultural importance indices: a comparative analysis based on the useful wild plants of Southern Cantabria (Northern Spain) Ethnobotanical study of medicinal and aromatic plants used in the treatment of genito-urinary diseases in the Moroccan Rif A preliminary classification of the healing potential of medicinal plants, based on a rational analysis of an ethnopharmacological field survey among Bedouins in the Negev Desert Medicinal plants in Mexico: Healers' consensus and cultural importance COVID-19, prevention and treatment with herbal medicine in the herbal markets of Salé Prefecture Insights into Eucalyptus genus chemical constituents, biological activities and health-promoting effects G: Costs and benefits of secondary metabolites to the Leguminosae Ethnobotanical and ethnopharmacological study of medicinal and aromatic plants used in the treatment of respiratory system disorders in the Moroccan Rif Ethno-medicinal studies on medicinal plants used by people of Rif, Morocco An ethnobotanical survey of indigenous medicinal plants in Hafizabad district Descriptive study of plant resources in the context of the ethno-medicinal relevance of indigenous flora: a case study from Toli Peer National Park Ethno-veterinary uses of Poaceae in Anti-proliferative and antioxidant properties of rosemary Rosmarinus officinalis Antibacterial and resistance modifying activity of Rosmarinus officinalis Antioxidative constituents of Rosmarinus officinalis and Salvia officinalis Ethnobotanical uses of medicinal plants for respiratory disorders among the inhabitants of Gallies-Abbottabad Ethnobotanical survey of medicinal plants used by Bangladeshi traditional health practitioners in the management of diabetes mellitus Ethnobotanical study and biodiversity of medicinal plants used in the Tarfaya Province Pharmaceutical ethnobotany in the western part of Granada province (southern Spain): ethnopharmacological synthesis Informant consensus factor and antibacterial activity of the medicinal plants used by the people of San Rafael Coxcatlán Medicinal plants used for diabetic problems in the Rif Ethno-medicinal and Ethnoveterinary Plants from Boath Ethnobotanical study of medicinal plants used by local people in Ofla Wereda Utilização de plantas medicinais pela comunidade atendida no programa saúde da família da Pirajá Ethno-medicinal plants and traditional knowledge among three Chin indigenous groups in Natma Taung National Park (Myanmar) Ethnobotanical study of some Ghanaian anti-malarial plants Ethnobotanical survey of medicinal plants used in the treatment of animal diarrhoea in Plateau State Traditional Arabic and Islamic medicine: a conceptual model for clinicians and researchers Traditional Arabic and Islamic medicine: validation and empirical assessment of a conceptual model in Qatar Antibacterial, antioxidant and cytotoxic studies of total saponin, alkaloid and sterols contents of decoction of Joshanda: identification of components through thin layer chromatography Les plantes médicinales dans la forêt de l'achach Catalogue des plantes medicinales utilisees dans la region de Zaër (Maroc Occidental) Quantitative analyses of medicinal plants consumption among the inhabitants of Shangla-Kohistan areas in Northern-Pakistan Ethnobotanical study aimed at investigating the use of medicinal plants to treat nervous system diseases in the Rif of Morocco Survey of medicinal plants used to treat malaria by Sidama People of Boricha District, Sidama Zone, South Region of Ethiopia Ethnobotanical study of medicinal plants in Ganta Afeshum District Plants used by the rural community of Bananal, Mato Grosso, Brazil: aspects of popular knowledge An ethnobotanical survey of medicinal plants in Edremit Gulf (Balıkesir-Turkey) Acknowledgments We wish to send our honest thankfulness to all the guides and inhabitants of the Rif region for their help. To all sellers of medicinal plants (Attar). We also extend our acknowledgments to all those who participated in the achievement of this product.