key: cord-0784736-1y46p2mr
authors: de Latour, Regis Peffault; Bergeron, Anne; Lengline, Etienne; Dupont, Thibault; Marchal, Armance; Galicier, Lionel; de Castro, Nathalie; Bondeelle, Louise; Darmon, Michael; Dupin, Clairelyne; Dumas, Guillaume; Leguen, Pierre; Madelaine, Isabelle; Chevret, Sylvie; Molina, Jean-Michel; Azoulay, Elie; Fremeaux-Bacchi, Veronique
title: Complement C5 inhibition in patients with COVID- 19 - a promising target?
date: 2020-12-01
journal: Haematologica
DOI: 10.3324/haematol.2020.260117
sha: 805eb1b8c4180b8039506bbc232fd408e60a6227
doc_id: 784736
cord_uid: 1y46p2mr

nan

Since the report of the first cases in December 2019, infection with the severe acute respiratory coronavirus 2 (SARS-CoV-2) commonly referred as COVID-19 has become a worldwide pandemic. 1 In patients with COVID-19 infection, respiratory deterioration has been associated with not only increased viral loads in the lung but also with an inadequate and exaggerated immune response. 2 Preclinical data have demonstrated a role for complement activation in CoV-mediated disease. Gralinski et al., found activation of the complement system in a mouse model of CoV. 3 In some patients with COVID-19, significant deposits of terminal complement components C5b-9 (membrane attack complex), C4d, and mannose binding lectin (MBL)-associated serine protease 2 (MASP-2) have been found in the microvasculature of different organs, consistent with sustained, systemic activation of the lectin-complement pathway. 5 MASP-2 mediated complement overactivation has also been reported in some patients by a Chinese group. 6 We assessed complement activity in 103 patients with COVID-19 followed in the Saint-Louis hospital (Pneumology Unit, Infectious Disease Unit and Intensive Care Unit [ICU]). We used a validated routine complement hemolytic activity assay (reported as CH50) which consists of testing the capacity of patient plasma to lyse sheep erythrocytes coated with antibodies, C3, C4 and sC5b-9 circulating levels by nephelometry (Siemens) and ELISA (Quidel, San Diego, CA, USA), respectively, according to the manufacturers' instructions. We found that the levels of C3 and C4 were increased in 57.2 % (59 of 103) and 36.9 % (38 of 103) of patients, respectively (data not shown). Moreover, the level of circulating sC5b-9 was increased in 64% of the patients (66 of 103), highlighting the systemic C5 cleavage during Covid-19 infection (healthy controls median 160 ng/mL, range: 49-362 vs. Covid-19 patients median 344 ng/mL, range: 71-883, P<0.0001). We then classified the severity of COVID-19 patients as moderate (dyspnea requiring a maximum of 3 L/min of oxygen and no other organ failure), severe (respiratory distress requiring more than 3 L/min of oxygen and no other organ failure) and critical cases (respiratory failure requiring mechanical ventilation or high flow oxygen support, shock and/or other organ failure necessitating intensive care unit [ICU] care). The plasma levels of sC5b9 were significantly higher in the three groups of patients than in the heathy donors (P<0.001 in the three groups) and higher in the patients with critical disease than in moderate disease (P=0.01) ( Figure 1 ). Altogether, these findings suggest that C5 activation might be associated with disease severity, even if we agree causality still needs to be investigated. Nevertheless, this result has to be taken with caution since sC5b-9 may not properly reflect the situation on a cellular level. Moreover, tissue biopsies were not performed in any of our patients and the presence or absence of complement deposition in the lungs or microvasculature could, thus not be assessed.

Taken together, however, these findings suggest that complement might be targeted for specific intervention. In China, two deteriorating patients were rescued using an anti-C5a monoclonal antibody. 6 In Italy, four patients with severe pneumoniae successfully recovered after treatment with eculizumab. 8 In the absence of proven effective therapy, we decided to treat COVID-19 patients with severe pneumonia with eculizumab on an emergency compassionate-use basis. Five patients with severe pneumonia requiring ≥ 5L/min of oxygen to maintain SpO 2 >97% (but not requiring ICU) and three patients with respiratory failure requiring mechanical ventilation and suffering from renal injury (defined by AKI ≥2 or requiring dialysis) and vasopressive drugs support thus received eculizumab off label on a compassionate-use basis. All patients had confirmed severe COVID-19 using specific RT-PCR (positive PCR on nasal swabs). Characteristics of the patients are detailed in Table 1 . This report is based on data from patients who received haematologica | 2020; 105 (12) 2847

Letters to the Editor haematologica | 2020; 105 (12) Letters to the Editor given intravenously at dose of 20 mg once daily from day 1 to day 5, and then 10 mg once daily from day 6 to day 10. *At day +1 after injection, seven patients with available samples showed a decreased of circulating levels of sC5b9. The median sC5b9 levels pre (463 ng/mL) and post (317 mg/mL) Eculizumab was significantly different (P=0.01) using non-parametric statistical analysis (Wilcoxon test). However, no correlation between the percentage reduction and response to Eculizumab was found.

eculizumab during the period from March 17, 2020 through May 19, 2020. The first ICU patient (patient 1) was treated according to SOLIRIS ® SmPC -dosing regimen of atypical Hemolytic and Uremic Syndrome (aHUS, induction period with 900 mg every week). He continues to be monitored closely with regard to the complement activity during follow-up according to our usual practice. 9 The level of free eculizumab in the plasma was assessed using in house ELISA as previously described. 9 At day 7, we observed a lack of complete inhibition of C5 with normal CH50 activity and undetectable free eculizumab circulating levels in patient 1. These findings suggest a much higher clearance of eculizumab in patients with COVID-19 than usually observed after a single injection in other diseases like aHUS and paroxysmal nocturnal hemoglobinuria. 9 Patients 2, 3 and 5 thus received 900 mg every 4 days allowing better but not optimal and prolonged complement blockade. We observed low eculizumab levels at day 4 (below 50 mg/mL in 2 of the 3 patients) with an efficient complement inhibition since day 1. The next four patients thus received three induction doses of 1,200 mg at day 1, 4 and 8, which appears satisfactory on a PK/PD standpoint (CH50 blocked before reinjection and residual free eculizumab upper to 50 mg/mL in all evaluable patients). Because of the complement blockade the patients received prophylactic antibiotics against meningococcal infection prior to initiating eculizumab treatment and were vaccinated when possible.

At time of eculizumab initiation, three patients were intubated, one received high flow oxygen and four patients were treated with standard oxygen support only. They all had elevated sC5b-9 circulating levels. Over a median follow-up of 19.5 days (range: 13-31 days) after receiving the first dose of eculizumab, six patients showed an improvement in the category of oxygen support, including one patient receiving mechanical ventilation who was subsequently extubated. At last follow-up, six patients had been discharged (day +5, +13, +13, +23, +34, +40 days after first eculizumab injection, respectively). Two patients who received invasive ventilation died (day +4 and +10 after the first eculizumab injection, respectively). Patient 5 presented a septic shock and multi organ failure while patient 6 was diagnosed with massive pulmonary embolism and cardiac arrest. In addition, patient 1 also presented severe thrombotic complications during evolution (deep venous thrombosis and pulmonary embolism). Those latter observations are in line with the recent findings suggesting that up to 30% of patients with severe COVID-19 infection develop life-threatening thrombotic complications. 10 Those considerations reinforce the recommendation to strictly apply thrombosis prophylaxis in COVID-19 patients, 11 also in the context of complement inhibition. While the relationship between thrombosis occurrence and complement activation is still unclear in patients with COVID 19, evidence is emerging that innate immunity contributes to the inflammatory storm that leads to respiratory failure in many patients with COVID-19. In particular, neutrophils-derived neutrophil extracellular traps (NET) which are extracellular webs of DNA, histones, microbicidal proteins, and oxidant enzymes that are released by neutrophils to corral infections have the potential to propagate inflammation and thrombosis if not properly regulated 12,13 as is the case in patients with COVID 19. 14 Excessive inflammation together with massive complement activation might thus participate in the predisposition of patients to a higher thrombotic risk.

Overall, we showed that the terminal pathway of the

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Acknowledgments: the authors would like to thank the colleagues who actively participated in the study and the laboratory team in HEGP who performed the complement analysis. We would particularly like to thank the patients for kindly accepting to take part in this research.

complement is overactivated in 64% of COVID-19 patients on admission to hospital. This may contribute to the severity of the disease. Naturally, it is difficult to draw any robust conclusion on the efficacy of complement blocking, firstly because of the small number and heterogeneity of our patients, and secondly in the absence of a control group.However, all of our eight patients were particularly severe at the time of eculizumab initiation and six improved significantly. Even if further investigation is needed, our results suggest that the inhibition of one mechanism of COVID-induced organ damage may be an add-on treatment for this condition.Our experience also highlights that eculizumab pharmacokinetics in COVID patients differ from reports in other complement-mediated diseases. In this particular disease, higher eculizumab doses and/or shorter intervals to ensure an efficient and sustained blockade seem required. The degree of complement activation at the start of therapy, possibly including C5b9 deposition in the tissue may be a significant determinant of eculizumab clearance and disease response. Controlled trials are timely to confirm the value of eculizumab in patients with COVID-19.Regis Peffault de Latour, 1,2 Anne Bergeron, 2,3,4 Etienne Lengline, 5, 6 Thibault Dupont, 6 Armance Marchal, 7 Lionel Galicier, 8 Nathalie de Castro, 9 Louise Bondeelle, 3 Michael Darmon, 2,6 Clairelyne Dupin, 3 Guillaume Dumas, 6 Pierre Leguen, 3 Isabelle Madelaine, 3