key: cord-0784674-7a4i1qty
authors: Yin, Lijuan; Man, Shuli; Ye, Shengying; Liu, Guozhen; Ma, Long
title: CRISPR-Cas based virus detection: recent advances and perspectives
date: 2021-08-08
journal: Biosens Bioelectron
DOI: 10.1016/j.bios.2021.113541
sha: 106fe698683675d45e39b7322e38c6924ffef1d8
doc_id: 784674
cord_uid: 7a4i1qty

Viral infections are one of the most intimidating threats to human beings. One convincing example is the coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2. Rapid, sensitive, specific and field-deployable identification of causal viruses is critical for disease surveillance, control and treatment. The shortcomings of current methods create an impending need for developing novel biosensing platforms. CRISPR-Cas systems, especially CRISPR-Cas12a and CRISPR-Cas13a, characterized by their sensitivity, specificity, high base resolution and programmability upon nucleic acid recognition, have been repurposed for molecular diagnostics, surging a new path forward in biosensing. They, as the core of some robust diagnostic tools, are revolutionizing the way that virus can be detected. This review focuses on recent advances in virus detection with CRISPR-Cas systems especially CRISPR-Cas12a/Cas13a. We started with a short introduction to CRISPR-Cas systems and the properties of Cas12a and Cas13a effectors, and continued with reviewing the current advances of virus detection utilizing CRISPR-Cas systems. The significance and advantages of such methods were then discussed. Finally, the challenges and perspectives were proposed. We tried to provide readers with a concise profile of emerging and fast-expanding CRISPR-Cas based biosensing technology, and highlighted its potential applications in a range of scenarios with regard to virus detection.

Description and comparison of Cas9, Cas12a, and Cas13a effectors. The fluorescence based sensing usually renders ease, lower background, and higher signal- Cas12a was silenced, the MB conjugated ssDNA was retained on the sensor surface (Fig. 3C ). also has been applied for virus detection in real samples, such as plasma, serum, urine, swabs,

Proc. Natl

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