key: cord-0784080-im8wdf0s authors: Tabassum, Rukhsana; Ashfaq, Muhammad; Oku, Hiroyuki title: 7-Hydroxy-4-phenyl-1, 2-dihydroquinoline derivatives: synthesis via one-pot, three-component reaction and structure elucidation date: 2020-10-01 journal: Heliyon DOI: 10.1016/j.heliyon.2020.e05035 sha: cc9ed6696db1812e89676816993e511edb7c6bc9 doc_id: 784080 cord_uid: im8wdf0s We have developed a new and facile one pot three component protocol catalyzed by ammonium acetate for construction of new functionalized 7-hydroxy-4-phenyl-1,2-dihydroquinoline derivatives. A variety of quinoline derivatives were obtained in good to excellent yield from inexpensive reagents and catalyst in mild reaction conditions that provide atom economy and cost efficacy. Various spectroscopic techniques like FTIR, (1)HNMR and (13)CNMR were employed to study their structure while mass of the synthesized compounds were confirmed through MALDI-TOF-MS and EI mass spectrometry. In recent years one pot multicomponent reactions attracted great attention due to simplicity, atom economy, and greater efficiency with less wastage of products during separation due to reduced number of intermediate purification steps that increase the yield of target products. These reactions may proceed through one step protocol or by many successive steps with generation of diversity of heterocyclic compounds in one pot [1, 2] . Multicomponent one pot reactions due to their efficiency and effectiveness provide a prevailing platform to access sustainable, complexity as well as diversity oriented synthesis of heterocyclic compounds from simple and inexpensive starting materials [3, 4, 5, 6, 7, 8, 9, 10] . Quinoline derivatives are heterocyclic compounds that are used as selective and potent drugs against many diseases. They show excellent antiviral activity against dengue virus [11] , zika virus [12] , avian influenza virus [13] and now in recent pandemic conditions against COVID-19 [14, 15, 16, 17] . They also possess pharmacological properties such as anti-inflammatory [18, 19, 20] , antimalarial [21, 22, 23, 24, 25] , anti-cancer [26, 27, 28, 29, 30, 31] , anti-hypertension [32] , anti-bacterial [33, 34, 35, 36, 37] , anti-tubercular [38, 39, 40, 41, 42, 43] , anti-fungal [33, 44, 45, 46, 47] , anti-urease [48] , anti-Alzheimer [49] , anti-diabetic [50] and anti-oxidant [51, 52, 53] . Quinoline moiety is a vital part of various natural and pharmacologically active compounds exhibiting a wide-range of biological activities [54, 55, 56] . Owing to their vital role in medicinal agents and natural products, quinolines have become important synthetic targets for chemists. A diverse range of one pot multicomponent protocols including both catalytic and non-catalytic synthetic methods have been developed that use diverse starting materials to construct the quinoline ring with virtually boundless combinations of functionality [10, 57, 58, 59, 60, 61, 62, 63] . L-proline as catalyst was reported for the synthesis of 4-phenyl quinoline derivatives from anilines but reaction time was 8-12h [58] so we use ammonium acetate as catalyst in one pot protocol which reduced the reaction time to 20min-90min. Ammonium acetate is used as catalyst as well as reagent in synthesis of a wide range of heterocyclic compounds [64, 65] . In this paper we report a quick one pot, three component facile reaction of malononitrile, 3-aminophenol and substituted aldehydes for the synthesis of quinoline derivatives catalyzed by ammonium acetate. Our study instigated with optimization of reaction conditions by choosing benzaldehyd (1a) malononitrile (2) and 3-amino phenol (4) as model substrates in the absence and presence of different catalysts. The results are summarized and enlisted in Table 1 . Surprisingly we got 4phenyl-1,2-dihydroquinoline products instead of 4-phenyl quinoline derivatives as confirmed by EIMS and 1 HNMR. At the beginning we tried to obtain product without using any catalyst and to our delight 35% of product was separated in 1 h at reflux temperature (entry 1 Table 1) . Intermediate 3 was not obtained by the reaction of 1a and 2 but after addition of 4, at first intermediate 3 was produced at room temperature then on reflux it yielded the product 5a, and from these results it was concluded that catalyst is necessary and 3-aminophenol itself acted as catalyst for the reaction. So to increase the yield we tried different catalyst and recorded results in Table 1 and found that 25 mol% of K 2 CO 3 in ethanol and water (4:1) solvent system yielded 43% of product 5a when reaction was proceeded through two steps that is firstly 1a allowed to react with 2 at room temperature and then solution of 4 was added at reflux temperature while 40% yield was obtained when all reactants were added together (one step multicomponent protocol) (entry 2 Table 1 ). Next we used Et 3 N 10 mol% and 20 mol% (Table 1 entry 3 and 4 ) but the yield was not exceeded from 50% and 46% through two steps and one step reaction respectively. With L. proline (Table 1 entry 5) reaction was not accomplished and reactants 1a and 2 remains as such even after 12 h of stirring. As from above results it was clear that adding all reactants together decrease the yield to some extent and it may be due to the involvement of 4 as catalyst in the production of benzylidenemalononitrile (3a), we further investigated other catalyst only through two steps reactions. We tried different ammonium salts (Table 1 entries 6-8) as catalyst and amazingly got 65% yield of product with ammonium acetate in 1 h at reflux temperature. Then we used different quantities of ammonium acetate and best result was obtained with 30 ml% of ammonium acetate (Table 1 entry 11) which was 95% yield of product in just 20 min (5min stirring at room temperature (step 1) and 15 min stirring at reflux temperature (step 2)). After the selection of appropriate catalyst we further investigated different solvents for reaction (Table1 entries 13-18) Reaction was not proceeded in water that might be due to the insolubility of 1a and 4 in water while in methanol inspite the solubility of reactants 1a did not react with 2 to yield solid product rather slight change in color occurred which when monitored with TLC showed a third component in reaction medium beside 1a and 2 but when 4 was added in reaction mixture no product was separated at all. Ethanol was found to be the most suitable solvent for reaction as product separated during heating and no further purification was required except washing the product with hot ethanol. With optimized reaction conditions (Table 1 entry 11) in hand we further evaluated the scope and generality of substrates for this one pot reaction and results are enlisted in Figure 1 . A variety of aldehydes, nonsubstituted (1a, 1g) and substituted with electron withdrawing groups such as NO 2 (1e, 1f), Cl (1h, 1k), Br (1d, 1m), F (1J) and electron donating groups like OCH 3 (1b, 1c), CH 3 (1l, 1o) and OH (1i, 1n) reacted smoothly with 3-amino phenol (4) and malononitrile (2) and furnished the corresponding 4-phenyl quinoline derivatives in good to excellent yield (63-97%) under mild reaction conditions. Non-substituted aldehydes 1a and 1g produced the required products 5a and 5g in highest yields which are 95% and 97% respectively in just 20 min (1 st step in 5minuts and second step in15 min). Substitution on aldehydes effected the time of completion of reaction for example non substituted and para substituted aldehydes converted into products in 20 min. while meta substituted aldehydes took 40 min. for completion of reaction and for ortho substituted aldehydes longest time was required to convert the reactants into desired product (90 min.). Beside the effect on time of reaction ortho substitution on aldehydes irrespective of activating or deactivating group also decreased the yield of corresponding quinoline derivatives 5 as compared to meta and para substitution. This effect may be aroused due to steric hindrance of groups present at ortho position. Methoxy (OCH 3 ) group at meta position of aldehyde (1c) produced excellent yield of product 5c (86%) as compared to para methoxy benzaldehyde (1b) which 77% converted into product 5b while ortho methoxy benzaldehyde did not produced the desired product at all. Methyl group showed an opposite effect than that of OCH 3 as its presence at para position gave excellent yield (94%) of 5l. NO 2 group at meta position of 1 furnished the product 5f in 87% yield while para nitro group produced lesser yield (71%) of product. Halogen groups (F, Cl, and Br) produced 95%, 87% and 81% of required products irrespective of the position of substituents while OH at para position resulted in higher yield (72%) of products as compared OH at meta position (68%). Some aldehydes did not yielded the products, for example para dimethyl amino benzaldehyde only furnished the intermediate 3 and did not converted into product 5, similarly heterocyclic aldehydes like 2-pyridine aldehyde, furfural aldehyde did not reacted to produce the corresponding products. This multicomponent one pot protocol furnished 2-amino-7-hydroxy-4-aryl-1,2-dihydroquinoline-3-carbonitrile derivatives confirmed by NMR studies as represented in elaborated 1 HNMR spectra of 5b ( Figure 2 ) that clearly indicated that Hb showed a doublet peak due to coupling with ortho Hc with 8.2 Hz coupling constant while Hb showed doublet of doublet (J ¼ 8.2, 2.4Hz)) as it has one ortho (Hc) and one meta (Ha) neighboring proton. Ha coupled with Hb which is at its meta position and showed a doublet with J ¼ 2.4 Hz. Hd and He coupled with their ortho as well as meta protons giving doublet of doublet with coupling constant 6.7, 2.2 Hz and 6.5, 2.1 Hz respectively. These studies showed that Michael addition take place at position 1 of 3-aminophenol which is more reactive than position 2 ( Figure 3 ) and thus the product obtained was more stable in accordance with Ali Khalafi-Nezhad et al. who explained it on the basis of NPA charges calculations (Figure 3 ), thermodynamics and steric hindrance [58] . They also explained the oxidation process in 3-carbonitrile derivatives which helped to aromatize the product but in our case we obtained 1,2-dihydroquinoline derivatives instead of 1,4-dihydroquinoline derivatives so aromatization did not take place in products we obtained as reported in Figure 1 . FTIR spectra of all synthesized compounds were recorded in 4000 cm À1 to 600 cm À1 domain and all spectra have prominent peaks characteristic of synthesized quinoline derivatives such as NH 2 , NH, OH, CN, C¼C, C-N and C-O peaks in the vibrational range of 3500-1000 cm À1 (Figures S1-S15). NH 2 and NH peaks appear in the range of 3500-3300cm À1 while CN showed peaks in the region of 2200-2100cm À1 and OH peaks appear as broadened NH 2 peak from 3500-3000cm À1 in all spectra of compounds 5a-o. In exemplary spectra of 5a, 5b, 5i and 5l ( Figure 4 ) CN stretching vibration appears as sharp and strong peak at 2197.78, 2188.23, 2182.20 and 2190.48 cm À1 respectively. NH 2 vibrations appear as strong double peaks (asymmetric and symmetric stretch) in all spectra while NH vibration overlapped by NH 2 vibration in 5a and is not visible while it is visible in 5b and 5l and in 5i broad two OH groups peak overlapped it as well. Sp 3 CH stretching signals can also be clearly seen in spectra of 5b and 5l. Strong stretching band in the region of 1650-1400 cm À1 are assigned to C¼C groups of aromatic ring of reported compounds. All these peaks are in accordance with the proposed structure of reported compounds. In 1 HNMR and 13 CNMR spectra of all synthesized compounds (5a-5o) all required signals conforming the respective proton and carbon nuclei of suggested structures are observed at agreeing chemical shifts (ppm) values which helped in unambiguous characterization of 7-hydroxy-4phenyl-1,2-dihydroquinoline derivatives ( Figures S16-S30 ). Experimental spectra are almost in accordance with theoretical spectra obtained by using Chem draw ultra 12.0. Exemplary 1 HNMR of 5a ( Figure 5C ) allows us to identify different proton's chemical shift values, for example, signal at 4.49 ppm (s) is assigned to CH attached to NH 2 while overlapped singlet peak of two protons at 5.20 ppm is of NH 2 , peaks originating due to H a , H b and H c are observed at 6.19 ppm (d, J ¼ Figure 6 . 13 CNMR spectra of 5a A) experimental B) Theoretical. 2.7 Hz), 6.24 ppm (dd, J ¼ 8.2, 2.1 Hz) and 6.59 ppm (d, J ¼ 8.2 Hz) respectively and aromatic protons of Ar produce signals in the range of 7.25 ppm (t, J ¼ 7.6 Hz, 2H) and 7.16-7.11ppm (m, 3H). NH, and OH signal appear at 6.71ppm as singlet strong peak of two protons. Experimental 1 HNMR spectra is almost in accordance with predicted 1 HNMR spectra ( Figure 5B ) that confirms the formation of our required products. Similarly 13 CNMR of 5a ( Figure 6A ) showed distinct resonances all corresponding to respective carbon nuclei. Carbon bonded to NH 2 group resonated at 56.96 ppm while the signals of carbon atom attached with nitrile group is evident at 100.68 ppm and aromatic carbon attached to OH group appear at 160.95 ppm. In addition quaternary carbon C10 attached to nitrogen of pyridine ring resonate at 149.29 ppm. Carbon of nitrile group appear at 129.96 ppm. All other carbon nuclei also appear in aromatic region which are in accordance with predicted 13 CNMR of 5a ( Figure 6B ). 13 CNMR of all other 1,2-dihydroquinoline derivatives showed same trend of peaks which confirms the formation of our desired products ( Figures S31-S45 ). Mass of the synthesized compounds were determined by EI-MS using electron ionization (Figures S61-S63 ) and MALDI-TOF-MS by using Table 2 . Observed peaks in MALDI-TOF-MS spectra of 5a-o. Alpha-Cyano-4-hydroxycinnamic acid (α-CHCA) as matrix in positive ion, reflectron mode ( Figures S46-S60) . MALDI-TOF uses soft ionization method to generate ions and is well known as a high throughput technique [66] . Previously it was mostly used for compounds with higher molecular weight but now a days it is also employed for compounds with low molecular weight with great success [67, 68] . Surprisingly [M-H] þ and M þ• peaks were observed in almost all investigated compounds instead of [MþH] þ peaks which are observed only in 5c ( Figure S48 ) and 5d ( Figure S49 ) spectra, this indicated that these type of compounds produce (Table 2 ). [M-H] þ peak is observed in all spectra which may appear due to; a) transfer of hydrogen atom from radical cation of analyte or b) by hydride removal from neutral molecule of analyte, or c) removal of H 2 from protonated molecule of analyte [69, 70, 71, 72] All these three mechanisms may occur simultaneously or not depending on the structure of analyte molecule [73] . M þ• observed in spectra of 5b, 5d, 5h, 5k and 5m ( Figures S47, S49 Similarly EI-MS spectra of some selected compound like 5a, 5b and 5f were recorded in positive ion mode using JEOL-600H-1 mass spectrometer which confirmed the results obtained from MALDI-TOF spectra and molecular mass of synthesized compounds. EI-MS spectrum of 5a ( Figure 7B) Based on the experimental results and previous literature [65, 74, 75] a proposed mechanism of the reaction was shown in Scheme 1. According to proposed mechanism acetate ion facilitate the removal of H from 2 to yield A while ammonium ion catalyzed the formation of iminium ion (B) which due to higher reactivity than carbonyl group expedite the Knoevenagel condensation between A and B followed by elimination of ammonium ion to yield benzylidenemalononitrile (C). Then C through Michael addition with intermediate D produced E which by imine-enamine rearrangement and cyclization yielded F and G respectively. Intermediate G may exist in three possible forms that are G, H and 5 out of which 5 (7-hydroxy-4-phenyl-1,2-dihydro quinoline derivatives) is most stable as predicted by spectroscopic data. In conclusion, we successfully synthesized 7-hydroxy-4-phenyl-1,2dihydroquinoline derivatives (5a-o) by a convenient one pot, three component protocol in 63-97% yield. The reaction proceed though Knoevenagel condensation, Michael addition, rearrangement and cyclization. Notably, this protocol proceeded without the use of toxic solvents, co-catalysts, precious metals, inert atmosphere and harsh reaction conditions. This one pot atom economic method eliminates the wastage of products and exhibits wide range of functional group tolerance with high substrate scope under mild reaction conditions with excellent yield of corresponding products as high as 97%. Furthermore, structure of all synthesized compounds were elucidated by various techniques including FTIR, 1 HNMR, 13 CNMR, MALDI-TOF-MS and EI-MS which confirmed that the obtained products are 7-hydroxy-1,2-dihydroquinoline derivatives instead of 7-hydroxy quinolines. Further applicability of this protocol to a wide variety of substrates and bio screening of these compounds are in progress in our laboratory. All reagents were commercially available which were used without further purification. Melting points were recorded by open capillary method on Stuart SMP10 and are uncorrected. Silica gel plates were used to monitor the progress of reaction and purity of compounds by thin layer chromatography technique using ethanol: n-hexane (1: 1), acetone: nhexane (1:1) and acetone: n-hexane (7:3) solvent system which were visualized under UV. FTIR spectra were recorded on Bruker Tensor 27 FTIR spectrophotometer using KBr discs. 1 HNMR and 13 CNMR spectra were recorded on JEOL DELTA2 NMR spectrophotometer operating at 600 MHz and Bruker DMZ NMR spectrophotometer operating at 300 MHz H1NMR data are reported as: chemical shift (multiplicity, coupling constant (Hz)). Multiplicity is represented as: s ¼ singlet, d ¼ doublet, t ¼ triplet, m ¼ multiplet. Mass spectra were recorded by MALDI-TOF-MS technique on Shimadzu Biotech Axima Performance mass spectrometer using Alpha-Cyano-4-hydroxycinnamic acid (α-CHCA) as matrix in positive ion, reflectron mode. EI-MS spectra were recorded in positive ion mode using JEOL-600H-1 mass spectrometer. To a solution of malononitrile 2 (0.33g, 5.0mmol) and ammonium acetate (0.116g, 30 mol %) in 10ml ethanol, appropriate benzaldehyde 1 (5.0 mmol) was added and stirred at room temperature for 5-10 min. Intermediate benzylidenemalononitrile 3 was obtained as solid product. Then the temperature of the reaction mixture was increased to 70 C which helped in dissolving 3 in ethanol. After getting clear solution of reaction mixture, solution of 3-amino phenol (0.545g, 5.0 mmol) in 10mL ethanol was added in it and refluxed for 15-90 min. The solid thus obtained was filtered and washed with hot ethanol to obtain highly pure corresponding product 5a-o in up to 97% yield. Rukhsana Tabassum: Performed the experiments; Analyzed and interpreted the data; Wrote the paper. Muhammad Ashfaq: Conceived and designed the experiments; Contributed reagents, materials, analysis tools or data. Hiroyuki Oku: Analyzed and interpreted the data. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. Facile and efficient synthesis of bicyclic ortho-aminocarbonitrile derivatives using nanostructured diphosphate Na 2 CaP 2 O 7 Synthesis and characterization of nanorod magnetic Co-Fe mixed oxides and its catalytic behavior towards one-pot synthesis of polysubstituted pyridine derivatives Multi-component reactions : emerging chemistry in drug discovery Recent developments in isocyanide based multicomponent reactions in applied chemistry A novel isocyanide-based three-component synthesis of benzimidazo A concise and diversityoriented strategy for the synthesis of benzofurans and indoles via ugi and DielsÀAlder reactions New catalyzed three-component cycloadditions for the synthesis of eight-membered carbocycles Utilisation of 1,3-dicarbonyl derivatives in multicomponent reactions The chemistry of isocyanides, their MultiComponent reactions and their libraries Recent developments in the isonitrile-based multicomponent synthesis of heterocycles Antiviral activity of novel quinoline derivatives against dengue virus serotype 2 Antimalarial drugs and their metabolites are potent Zika virus inhibitors 8-Hydroxyquinoline-2-Carboxanilides as antiviral agents against avian influenza virus Discovering drugs to treat coronavirus disease 2019 (COVID-19) In vitro antiviral activity and projection of optimized dosing design of hydroxychloroquine for the treatment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro Hydroxychloroquine and azithromycin as a treatment of COVID-19: results of an open-label nonrandomized clinical trial Design, synthesis, and biological evaluation of ketoprofen analogs as potent cyclooxygenase-2 inhibitors Pharmacological effects of a synthetic quinoline, a hybrid of tomoxiprole and naproxen, against acute pain and inflammation in mice: a behavioral and docking study Synthesis and biological screening of pyrano[3,2-c]quinoline analogues as anti-inflammatory and anticancer agents Biological activities of quinoline derivatives Quinoline hybrids and their antiplasmodial and antimalarial activities Antiplasmodial and antimalarial activities of quinolone derivatives: an overview 4-Anilinoquinoline triazines: a novel class of hybrid antimalarial agents Quinoline -a promising fragment in the search for new antimalarials Synthesis and biological evaluation of quinoline analogues of flavones as potential anticancer agents and tubulin polymerization inhibitors A review on anticancer potential of bioactive heterocycle quinoline Insight view on possible role of fluoroquinolones in cancer therapy A review on the antitumor activity of various nitrogenous-based heterocyclic compounds as NSCLC inhibitors Synthetic methods of quinoline derivatives as potent anticancer agents Imidazoquinolines: recent developments in anticancer activity Pharmacologic profiles of GA0113, a novel quinoline derivative angiotensin II AT1-receptor antagonist Quinoline derivatives bearing pyrazole moiety: synthesis and biological evaluation as possible antibacterial and antifungal agents Synthesis and antibacterial evaluation of certain quinolone derivatives 4-Quinolone derivatives and their activities against Gram positive pathogens Ultrasound assisted synthesis of quinoline derivatives in the presence of SnCl2⋅2H2O as a precatalyst in water: evaluation of their antibacterial activities Hybrid bioactive heterocycles as potential antimicrobial agents: a review Recent advances of pyrazole-containing derivatives as anti-tubercular agents Fluoroquinolone derivatives and their anti-tubercular activities Isatin hybrids and their anti-tuberculosis activity Quinoline, A promising antitubercular target Design, synthesis of quinolinyl Schiff bases and azetidinones as enoyl ACP-reductase inhibitors Se Won, Nitroimidazoles, quinolones and oxazolidinones as fluorine bearing antitubercular clinical candidates Design and biological evaluation of novel quinolone-based metronidazole derivatives as potent Cu2þ mediated DNAtargeting antibacterial agents Synthesis of novel quinoline-based 4,5-dihydro-1H-pyrazoles as potential anticancer, antifungal, antibacterial and antiprotozoal agents Hybrid molecules development: a versatile landscape for the control of antifungal drug resistance: a review Facile and solvent-free domino synthesis of new quinolidinyl-2,4-thiazolidinones: antifungal activity and molecular docking Sulfonamide-linked ciprofloxacin, sulfadiazine and amantadine derivatives as a novel class of inhibitors of jack bean urease; synthesis, kinetic mechanism and molecular docking Design, synthesis, and evaluation of orally bioavailable quinoline-indole derivatives as innovative multitarget-directed ligands: promotion of cell proliferation in the adult murine Hippocampus for the treatment of alzheimer's disease Synthesis and evaluation of quinoline carboxyguanidines as antidiabetic agents Synthesis and antioxidant properties of novel quinoline-chalcogenium compounds Ethoxyquin: an antioxidant used in animal feed Glycoconjugates of quinolines: application in medicinal chemistry Copper-catalyzed cascade addition route to 2,3,4-trisubstituted quinoline derivatives Quinine, an old anti-malarial drug in a modern world: role in the treatment of malaria Recent Developments on Antimicrobial Quinoline Chemistry Metal-free domino one-pot protocols for quinoline synthesis l-Proline-promoted three-component reaction of anilines, aldehydes and barbituric acids/ malononitrile: regioselective synthesis of 5-arylpyrimido[4,5-b]quinoline-diones and 2-amino-4-arylquinoline-3-carbonitriles in water One-pot synthesis of quinoline-2(1H)-thiones from 2-isocyanostyrenes via electrocyclic reaction of the corresponding 2-isothiocyanatestyrenes Molecular iodine-catalyzed multicomponent reactions: an efficient catalyst for organic synthesis An efficient onepot, three-component synthesis of indeno[1,2-b]quinoline-9,11(6H,10H)-dione, acridine-1,8(2H,5H)-dione and quinoline-3-carbonitrile derivatives from enaminones Metal-free synthesis of 2-difluoromethylated quinolines via DBU-promoted cascade michael addition/cyclization of methyl 4,4-difluorobut-2-ynoate with 2-aminobenzonitriles One-pot synthesis of substituted indolo[1,2-a]quinolines under transition-metal-free conditions The dual role of ammonium acetate as reagent and catalyst in the synthesis of 2, 4, 5-triaryl-1H-imidazoles Ammonium acetate catalyzed an efficient one-pot three component synthesis of pyrano[3,2-c]chromene derivatives High-throughput small molecule identification using MALDI-TOF and a nanolayered substrate A novel strategy for MALDI-TOF MS analysis of small molecules Small molecule MALDI MS imaging: current technologies and future challenges Dehydrogenation and dehalogenation of amines in MALDI-TOF MS investigated by isotopic labeling The unexpected formation of [M À H]þ species during MALDI and dopant-free APPI MS analysis of novel antineoplastic curcumin analogues Dehydrogenation of tertiary amines in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry The formation of [M-H]þ ions in N-alkyl-substituted thieno[3,4-c]-pyrrole-4,6-dione derivatives during atmospheric pressure photoionization mass spectrometry Analysis of low molecular weight compounds using MALDI-and LDI-TOF-MS: direct detection of active pharmaceutical ingredients in different formulations Magnetic nano cobalt ferrite catalyzed synthesis of 4H-Pyrano[3,2-h]quinoline derivatives under microwave irradiation A convenient one-pot synthesis and anxietic activity of 3-cyano-2(1H)-iminopyridines and halogen derivatives of benzo[h]chromenes, Arab The authors are very grateful for The Department of Chemistry, The Islamia University of Bahawalpur for providing the necessary research facilities. The authors declare no conflict of interest. Supplementary content related to this article has been published online at https://doi.org/10.1016/j.heliyon.2020.e05035.