key: cord-0779154-8o8q6cas authors: Song, Kyoung-Ho; Kim, Dong-Min; Lee, Hyunju; Ham, Sin Young; Oh, Sang-Min; Jeong, Hyeonju; Jung, Jongtak; Kang, Chang Kyung; Park, Ji Young; Kang, Yu Min; Kim, Ji-Yeon; Park, Jeong Su; Park, Kyoung Un; Kim, Eu Suk; Kim, Hong Bin title: Dynamics of viral load and anti-SARS-CoV-2 antibodies in patients with positive RT-PCR results after recovery from COVID-19 date: 2020-12-01 journal: Korean J Intern Med DOI: 10.3904/kjim.2020.325 sha: f2ccbbd7ca56b87eca6850c717c8f689185020b0 doc_id: 779154 cord_uid: 8o8q6cas Recently, the number of patients with coronavirus disease 2019 (COVID-19) who have tested positive for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), via the reverse transcription polymerase chain reaction (RT-PCR) test, after recovery has increased; this has caused a dilemma regarding the medical measures and policies. We evaluated the dynamics of viral load and anti-SARS-CoV-2 antibodies in four patients with positive RT-PCR results after recovery. In all patients, the highest levels of immunoglobulin G (IgG) and IgM antibodies were reached after about a month of the onset of the initial symptoms. Then, the IgG titers plateaued, and the IgM titers decreased, regardless of RT-PCR results. The IgG and IgM levels did not increase after the post-negative positive RT-PCR results in any of the patients. Our results reinforced that the post-negative positive RT-PCR results may be due to the detection of RNA particles rather than reinfection in individuals who have recovered from COVID-19. Dynamics of viral load and anti-SARS-CoV-2 antibodies in patients with positive RT-PCR results after recovery from COVID-19 Kyoung-Ho Song 1 Four patients had positive RT-PCR results for SARS-CoV-2 after discharge. Patient 1 was a 73-year-old woman diagnosed with COVID-19 after exposure to her son and daughter-in-law, who had COVID-19. She presented with a 2-day history of fever and a 1-day history of headache, cough, and a sore throat. The other three patients were a family, which consisted of a 33-year-old man (Patient 2), his 31-year-old wife (Patient 3), and their 18-month-old daughter (Patient 4). Three days after exposure to a patient with COVID-19, Patients 2 and 3 developed fever and a productive cough, and were diagnosed with COVID-19. At that time, Patient 4 was asymptomatic; however, she was screened for SARS-CoV-2 infection and tested positive. The day after diagnosis, she became febrile. Patient 1 was diagnosed with COVID-19 pneumonia and subsequently treated with lopinavir/ritonavir for 10 days. The other three patients did not receive any antiviral drug treatment. All patients were hospitalized and placed in isolation for 13 to 29 days. Clinical symptoms and signs resolved at least 6 days (range, 6 to 20) prior to discharge. All patients had two consecutive negative RT-PCR test results at least 24 hours apart before discharge, according to the national guidelines. Four days after discharge, Patient 1 developed a productive cough and was retested 2 days later, and the result was positive. Patient 4 developed a 37.7°C fever and a productive cough after 9 days of discharge and was retested; the RT-PCR test result was positive. Although her parents (Patients 2 and 3) had no symptoms, they were retested on the same day as their daughter, and both tested positive. Patient 1 was tested for other respiratory viruses using a multiplex RT-PCR test performed on a bronchoscopic alveolar lavage specimen, but no other viruses were detected. Patient 2, 3, and 4 were also tested for other respiratory viruses using the multiplex RT-PCR test using nasopharyngeal swab specimens. Patient 4 tested positive for adenovirus and bocavirus but her parents tested negative for all viruses. Epidemiological investigations did not reveal any secondary cases of COVID-19 linked to any of the four patients after their first discharge. The time course of SARS-CoV-2 levels in respiratory specimens and humoral immune response of each patient in relation to their clinical signs, symptoms, and RT-PCR tests are shown in Fig. 1 Other studies have shown that in patients who are asymptomatic at the time of the post-negative positive RT-PCR test, the humoral response may be weak and may develop gradually. However, IgM and IgG become detectable in all COVID-19 patients between the 3rd and 4th week of the onset of their clinical illness [3, 4] ; thereafter, IgM levels begin to decline and reach lower levels by the 5th week, while IgG antibodies persist beyond the 7th week [5] . In this study, IgG and IgM antibodies persisted in all patients, but there was no increase in the antibody titer after more than 4 weeks of the onset of the first symptoms. These results suggest that the redetection the virus is more likely to have been due to the presence of RNA particles in respiratory samples, rather than re-infection or reactivation of the live viruses. There are other studies that have reported patients with post-negative positive SARS-CoV-2 RT-PCR results days or weeks after recovery with previous negative results [6, 7] . However, RT-PCR tests cannot differentiate infectious viruses from non-infectious RNA [8] , and RNA detection does not necessarily signify that complete virus or active infection is present [9] . Prolonged "shedding" may reflect a lack of elimination of the nucleic acid from the tissues [8] . The report [2] from the KCDC showed negative test results in 108 cases after performing viral cell culture of respiratory specimens at the time of second diagnosis. In 23 cases, the serum samples of the first and second diagnoses were tested for the presence of neutralizing antibodies, and 96% of the cases tested positive. Further, in an animal (rhesus macaques) study, SARS-CoV-2 reinfection failed to demonstrate viral replication in all sites examined, suggesting that SARS-CoV-2 induced protective immunity against re-exposure [10] . Due to the retrospective nature of the study, it had the following limitations. The samples were not collected at fixed time-points because they were collected for clinical, and not research purposes. Stored samples were used to detect antibodies, so storage could have affected World Health Organization. Novel coronavirus (2019-nCoV) situation reports WHO, c2020 Findings from Investigation and analysis of re-positive cases KCDC, c2020 Interpreting diagnostic tests for SARS-CoV-2 Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study Profile of specific antibodies to SARS-CoV-2: the first report Case report: viral shedding for 60 days in a woman with COVID-19 Positive RT-PCR test results in patients recovered from COVID-19 SARS-CoV-2 shedding and infectivity Testing dilemmas: post negative, positive SARS-CoV-2 RT-PCR: is it a reinfection SARS-CoV-2 infection protects against rechallenge in rhesus macaques The Korean Journal of Internal Medicine Vol. 36, No. 1, January 2021 the test results. Further, we did not perform viral cultures or neutralizing antibody tests. However, based on previous studies and the antibody response during the primary infection and post-negative positive RT-PCRresult period in the four patients, our results suggest that the post-negative positive RT-PCR results may be due to the detection of RNA particles rather than re-infection in individuals who have recovered from COVID-19. No potential conflict of interest relevant to this article was reported.