key: cord-0778361-m74agsr8
authors: Jung, Seo-Yeon; Kang, Kyung Won; Lee, Eun-Young; Seo, Dong-Won; Kim, Hong-Lim; Kim, Hak; Kwon, TaeWoo; Park, Hye-Lim; Kim, Hun; Lee, Sang-Myeong; Nam, Jae-Hwan
title: Heterologous prime–boost vaccination with adenoviral vector and protein nanoparticles induces both Th1 and Th2 responses against Middle East respiratory syndrome coronavirus
date: 2018-06-07
journal: Vaccine
DOI: 10.1016/j.vaccine.2018.04.082
sha: 18c87ac63c04723a939961675d43ff749f929e87
doc_id: 778361
cord_uid: m74agsr8

The Middle East respiratory syndrome coronavirus (MERS-CoV) is a highly pathogenic and zoonotic virus with a fatality rate in humans of over 35%. Although several vaccine candidates have been developed, there is still no clinically available vaccine for MERS-CoV. In this study, we developed two types of MERS-CoV vaccines: a recombinant adenovirus serotype 5 encoding the MERS-CoV spike gene (Ad5/MERS) and spike protein nanoparticles formulated with aluminum (alum) adjuvant. Next, we tested a heterologous prime–boost vaccine strategy, which compared priming with Ad5/MERS and boosting with spike protein nanoparticles and vice versa, with homologous prime–boost vaccination comprising priming and boosting with either spike protein nanoparticles or Ad5/MERS. Although both types of vaccine could induce specific immunoglobulin G against MERS-CoV, neutralizing antibodies against MERS-CoV were induced only by heterologous prime–boost immunization and homologous immunization with spike protein nanoparticles. Interestingly, Th1 cell activation was induced by immunization schedules including Ad5/MERS, but not by those including only spike protein nanoparticles. Heterologous prime–boost vaccination regimens including Ad5/MERS elicited simultaneous Th1 and Th2 responses, but homologous prime–boost regimens did not. Thus, heterologous prime–boost may induce longer-lasting immune responses against MERS-CoV because of an appropriate balance of Th1/Th2 responses. However, both heterologous prime–boost and homologous spike protein nanoparticles vaccinations could provide protection from MERS-CoV challenge in mice. Our results demonstrate that heterologous immunization by priming with Ad5/MERS and boosting with spike protein nanoparticles could be an efficient prophylactic strategy against MERS-CoV infection.

Middle East respiratory syndrome coronavirus (MERS-CoV) is a zoonotic beta coronavirus that can infect several kinds of animals including humans, camels, and bats [1] . It is known to cause severe respiratory symptoms and to have a high mortality rate [1] . The key receptor for MERS-CoV infection, dipeptidyl peptidase 4 (DPP4), is widely distributed on human endothelial and epithelial cells [2] . Except for cases in Korea in 2015, most infections with MERS-CoV (82%) have occurred in the kingdom of Saudi Arabia. The total number of laboratory-confirmed cases of MERS-CoV infection is 2040 with 712 deaths related to MERS-CoV infection since September 2012. Thus, the human mortality rate of MERS-CoV infection is approximately 35% [3] .

The genome of MERS-CoV is single-stranded RNA that encodes 10 M), a surface glycoprotein (S, spike), which comprises S1 and S2, and five nonstructural proteins (ORF 3, 4a, 4b, and 5) [4] . The main viral protein is the spike protein, which binds to the cell surface receptor DPP4 during the viral entry stage via the receptorbinding domain (RBD) of spike subunit S1 [5] . Because the spike protein is the most immunogenic structural protein [6, 7] , the final goal of most current studies of MERS-CoV vaccines is to elicit neutralizing antibodies against this specific MERS-CoV spike protein.

Although several approaches to developing a MERS-CoV vaccine have been reported, there is no clinically approved vaccine for MERS-CoV. Previous studies have investigated viral vector-based vaccines [8] [9] [10] [11] [12] , subunit vaccines [13] [14] [15] [16] [17] , and DNA vaccines [18, 19] . Of these, vaccination using viral vectors or DNA immunization successfully generated neutralizing antibodies and protected against infection [12] . However, safety concerns about DNA vaccines and their weak induction of neutralizing antibodies plus the possibility of reduced efficacy of viral vector vaccines because of preexisting immunity against the viral vectors induced by repeated immunization cannot be ignored. Although protein subunit vaccines can induce neutralizing antibody, they usually elicit a lower level of cellular immune response which has close association with rapid viral clearance when infection occurs. In addition, subunit vaccines could not induce enough immune responses in host, resulting in failure to make long-term memory of antigen [20] .

Therefore, we used a heterologous prime-boost immunization strategy combining recombinant adenovirus serotype 5 delivering MERS-CoV spike protein gene (Ad5/MERS) and MERS spike protein nanoparticles, because both types of vaccine have been shown to be safe in human trials. The results of this study showed that this heterologous prime-boost immunization strategy induced good humoral and cellular immune responses including neutralizing antibodies and activation of Th1 cells against MERS-CoV, and could protect mice against MERS-CoV infection. Therefore, this combined immunization with recombinant Ad5/MERS and spike protein nanoparticles may avoid the hurdles of preexisting antibody induced by repeated viral vector immunization and weak Th1 cell responses induced by protein subunit immunization.

See the Supplemental data for Materials and Methods for details regarding Cell, Virus preparation and titration, MERS spike protein nanoparticles, SDS-PAGE and Immunoblot analysis, Recombinant Ad5, Electron microscopy, Enzyme-linked immunosorbent assay (ELISA), Plaque reduction neutralization test (PRNT), MERS-CoV infection, and Statistical analysis.

Six-week-old female specific-pathogen-free BALB/c mice were purchased from Dae Han Bio Link Co., Ltd., (Chungcheongbuk-do, Korea). Mouse experiments were performed in accordance with the relevant ethical guidelines and regulations established by the Korean Association for Laboratory Animals [21] . All mice were housed in specific-pathogen-free conditions with a standard light cycle (12 h light/dark) and maintained according to protocols approved by the Institutional Animal Care and Use Committee, Sungsim Campus, Catholic University of Korea. All mice were fed a normal fat (5%) diet (Harlan Laboratories, Livermore, CA, USA) and sterile water. Mice were randomly allocated to groups of six and immunized three times as indicated (Table 1 ).

MERS-CoV was provided by the Korean Centers for Disease Control and Prevention (National Control Number 1-001-MER-IS-2015001). All experimental procedures were performed in the Biosafety Level 3 facility of the Korea Zoonosis Research Institute at Chonbuk National University. The virus was passaged and titered on Vero E6 cells.

Spodoptera frugiperda Sf9 insect cells were obtained from the American Type Culture Collection and maintained in Insect-XPRESSTM medium. The MERS-CoV spike protein sequence was referred from NCBI reference sequence (Genbank accession No. AGN70962), and the nucleotide sequence was codon optimized for optimal expression in insect cells. Full length spike gene was cloned into pBacPAK8 baculovirus transfer vector. MERS-CoV spike proteins were produced in Sf9 cells infected with recombinant baculovirus. Spike proteins were purified using a combination of anion exchange and glucose affinity chromatography. Recombinant adenoviruses encoding the MERS spike protein and human DPP4 were purchased from Sirion Biotech (London, UK). The detailed production protocol is in Supporting Information (SI).

Groups of mice were immunized using heterologous (different vaccine candidates for priming and boosting) or homologous (the same vaccine candidate for priming and boosting) prime-boost immunization. The detailed immunization schedules and grouping are shown in Table 1 and Fig. 2A .

Mouse splenocytes were collected and isolated after mice were euthanized. Then, 3 Â 10 6 splenocytes were seeded into wells of an EliSpot plate for detection of IFN-c secreting T cells. To stimulate the splenocytes, 1.6 lg/well of MERS-CoV spike-specific peptide [22] was added to the culture medium to restimulate the splenocytes and then cultured for 1 day. After 2 days, cells were stained to detect spots positive for cytokines analyzed.

All values are expressed as mean ± standard deviation (SD). Statistical analyses of the data were performed using GraphPad Prism software (version 5.01; GraphPad Software, La Jolla, CA, USA).

Between-group differences were tested using one-way analysis of variance (ANOVA) with a post hoc Tukey honestly significant difference (HSD) test. A p < 0.05 was considered statistically significant.

Adenovirus serotype 5 for delivery of the full-length MERS spike protein gene (Ad5/MERS) was produced and tested for gene transduction efficiency in cell lines. HeLa cells and A549 cells were infected with 1, 10, or 50 MOI of Ad5/MERS. At 24 h after infection, lysates of each cell line were harvested and immunoblotted with a polyclonal anti-MERS spike protein antibody, produced in rats immunized with MERS spike protein nanoparticles formulated with alum. The lysates of HeLa and A549 cell lines infected with Ad5/MERS at over 10 MOI clearly contained the approximately 140-kDa MERS spike protein (Fig. 1A) . These data confirmed that Ad5/MERS-infected cells can express the MERS spike protein. In addition, full-length MERS spike protein containing the transmembrane domain (amino acids 1297-1320) was produced in an insect cell culture system (Fig. 1B) and was confirmed the expression by Western blot (Fig. 1B) . Electron micrographs of these spike protein nanoparticles formulated with alum showed that their mean diameter was around 80 nm, whereas that of spike protein nanoparticles not formulated with alum is around 35 nm (Fig. 1C ). Alumformulated MERS spike protein nanoparticles displayed a broader distribution of diameters than those not formulated with alum.

We immunized mice three times with Ad5/MERS and spike protein nanoparticles. The details of the combinations used for heterologous or homologous prime-boost immunization is indicated in Table 1 . The immunization and bleeding schedules are shown in Fig. 2A . The sera from immunized mice were collected and pooled at weeks 2, 5, and 7 after the first vaccination (priming) to analyze the MERS spike protein-specific antibodies by ELISA. At 7 weeks after the first immunization (2 weeks after the second booster), mice were sacrificed. At 2 weeks after the first vaccination, spike protein-specific total IgG could not be detected (Fig. 2B) . However, at 5 weeks after the first vaccination (2 weeks after the first booster), spike protein-specific total IgG was increased in most groups except the Ad5/MERS-spike protein group. Finally, at 7 weeks after the first vaccination (2 weeks after the second booster), all vaccinated groups showed induction of MERS spike protein-specific antibodies, whereas the control PBS and Ad5/GFP groups did not show any induction of MERSspecific antibodies ( Fig. 2B and 2C ). Both the spike protein nanoparticles and Ad5/MERS-spike protein groups showed higher antibody titers compared with the Ad5/MERS group and the spike protein-Ad5/MERS group (Fig. 2C) .

However, IgG 1 and IgG 2a showed different patterns of induction compared with total IgG (Fig. 2B and 2C ). In general, IgG 1 represents the Th2 response, which is more associated with humoral immune responses, and IgG 2a represents the Th1 response, which is more associated with cellular immune responses [23] . For IgG 1 , the titers in the homologous prime-boost groups (spike protein nanoparticles or Ad5/MERS) were about 50% and 30% higher than those of the heterologous prime-boost groups each (Ad5/MERSspike protein and spike protein-Ad5/MERS) at 7 weeks after the first vaccination (2 weeks after the second booster) (Fig. 2C) . For IgG 2a , the Ad5/MERS and Ad5/MERS-spike protein groups showed higher titers than the spike protein group. However, the spike protein-Ad5/MERS group did not show any induction of IgG 2a at The graph shows mean optical density (OD) ± standard deviation. Differences between groups were assessed by one-way ANOVA with post hoc Tukey HSD test for comparing multiple treatments; the significance of differences between groups are indicated by letters. Each group had n = 6 mice. All within-group samples were pooled and independently analyzed three times. 7 weeks after the first vaccination (2 weeks after the second booster), although it showed induction of total IgG and IgG 1 (Fig. 2B and  2C ).

Seven weeks after the first vaccination (2 weeks after the second booster), we collected mouse sera to evaluate the presence of neutralizing antibodies against MERS-CoV. Homologous spike protein nanoparticles and heterologous Ad5/MERS-spike protein immunized groups showed 50% of reduction in MERS-CoV when serum of each group was diluted at1:160 and 1:80 (Fig. 3) . Although homologous Ad5/MERS and heterologous spike protein-Ad5/MERS immunization could induce total IgG against MERS spike protein (Fig. 2B and 2C) , these groups had no detectable neutralizing antibodies (Fig. 3) .

To analyze MERS spike protein-specific T cell activation, we used an EliSpot assay. First, we stimulated splenocytes cultured from immunized mice with a CD8 + T cell-specific peptide (S291, KYYSIIPHSI) [22] from MERS spike protein. Interestingly, groups immunized with regimens including Ad5/MERS (homologous Ad5/MERS group and heterologous groups including Ad5/MERSspike protein and spike protein-Ad5/MERS) showed three times higher levels of IFN-c-secreting T cells than the homologous spike protein nanoparticles immunized group (Fig. 4) . Although the homologous spike protein group showed a slight increase in IFN-c-secreting T cells compared with the PBS group, this increase was not as significant as those in Ad5/MERS-immunized groups (Fig. 4) . Regardless of the number of immunizations with Ad5/ MERS, all groups including Ad5/MERS immunization clearly showed the induction of IFN-c-secreting T cells (Th1 cells) (Fig. 4) .

To investigate T cell responses further, we performed ELISA on splenocyte culture supernatants after stimulation with CD8 + T cell-specific peptide to measure the levels of various cytokines. Splenocytes from homologous Ad5/MERS and heterologous Ad5/ MERS-spike protein-or spike protein-Ad5/MERS-immunized mice showed the induction of IL-2, IFN-c, tumor necrosis factor (TNF)-a, IL-4, IL-5, granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-10, with some exceptions in the homologous spike protein group with respect to the induction of TNF-a (Fig. 5) . These data are generally consistent with those from the EliSpot analysis (Fig. 4) .

We selected two vaccination groups to test the protective effect of the vaccines against MERS-CoV challenge: the homologous spike protein group and the heterologous Ad5/MERS-spike protein group. The immunization schedules were as shown in Fig. 2A . The immunized mice were intranasally infected with 2 Â 10 5 PFU live MERS-CoV 5 days after inoculation with 1.0 Â 10 10 IU of Ad5/ hDPP4, which generates expression of hDPP4, the MERS-CoV receptor. After MERS-CoV infection, the control PBS and Ad5/GFP groups lost weight to about 94% of their starting weight. However, the vaccinated groups showed significantly less weight loss (to about 98% of their starting weight; p < 0.05) (Fig. 6A) . We Mice from the immunized groups were sacrificed to analyze IFN-c-secreting T cells. For this, 3 Â 10 6 splenocytes were seeded and treated with S291 peptide. The number of stained cells were calculated using the AID iSpot Fluorescent EliSpot Reader System of AID GmbH (Strassberg, Germany). Mean optical density ± standard deviations are shown. Significance was assessed using one-way ANOVA with post hoc Tukey HSD test for comparing multiple treatments, and significant differences between groups are indicated by letters. SFU: spot-forming unit. Each group had n = 6 mice. All within-group samples were pooled and independently analyzed three times. Fig. 5 . Induction of cytokines after MERS-specific T cell peptide treatment of splenocytes from immunized mice. Cytokine levels (pg/mL) in culture supernatants of splenocytes were measured. Three days after MERS-CoV-specific peptide treatment, supernatants were diluted threefold and analyzed by multiplex cytokine ELISA. Mean optical density ± standard deviations are shown. Significance was assessed by one-way ANOVA with post hoc Tukey HSD test for comparing multiple treatments, and significant differences between groups are indicated by letters. Each group had n = 6 mice. All within-group samples were pooled and independently analyzed three times. compared the histopathology of homologous-or heterologousvaccinated mice (spike protein nanoparticles or Ad5/MERS-spike protein) with that in nonvaccinated controls (PBS and Ad5/GFP). Lungs were collected 5 days after MERS-CoV infection. Although the mice began to regain weight at 3 days after infection, the severity of the lesions in their lungs was clear. PBS and Ad5/GFP groups showed the most severe lesions (Fig. 6B and 6C ). Lung histology sections were examined by three blinded observers (Fig. 6C ) and scored (from 0 to 3 points) based on the degree of inflammatory cell infiltration observed on hematoxylin and eosin-stained sections (Fig. 6B) . The spike protein and the Ad5/MERS-spike protein groups showed significantly milder pathology with MERS-CoV infection (Fig. 6C ).

Although the first MERS-CoV outbreak occurred in 2012, there is still no commercially licensed MERS-CoV vaccine for human use. Therefore, there has been much research on how to present effectively the main target antigen, spike protein. Previous studies have reported the effectiveness of DNA vaccines containing the spike protein gene [18, 19] , spike protein itself [13, 17] , RBD subunit vaccines [14] [15] [16] , and viral vectors including modified vaccinia virus Ankara and adenovirus [8] [9] [10] [11] [12] for immunization of mice or nonhuman primates. Subunit vaccines, such as spike protein and RBD protein, induced neutralizing antibody against MERS-CoV, indicating the induction of a humoral immune response, and the DNA vaccine triggered the activation of cytotoxic T cells, indicating the induction of a cellular immune response. However, subunit vaccines usually induce weak cellular immune and Th1 cell responses, whereas DNA vaccines induce weak humoral immune and Th2 cell responses [20, 23, 24] .

In this study, although Ad5/MERS induced spike proteinspecific antibodies, these antibodies showed weak neutralizing activity. In contrast, Ad5/MERS induced MERS spike proteinspecific IFN-c-secreting T cells, which indicated the activation of Th1 cells. Moreover, the levels of effector cytokines including TNF-a, IL-2, GM-CSF, and IFN-c that were produced after stimulation of cultured splenocytes with CD8 + T cell-specific peptide were higher in the Ad5/MERS-immunized groups. These cytokines are associated with both Th1 and Th2 immune responses. Therefore, the cytokine ELISA data indicate that Ad5/MERS immunization triggered activation of both Th1 and Th2 responses against MERS spike protein.

However, although the Ad5 vector has some advantages for use in development of vaccines, such as the induction of cellular immune responses and easy production [25] , previous studies have reported that repeated injections of recombinant Ad5 reduced the presentation of the targeted antigen to antigen-presenting cells because of the presence of preexisting antibody against Ad5, and that this preexisting antibody, which was induced by the first (priming) vaccination, prevented further immune responses. This could be because of the low titers of neutralizing antibody induced by Ad5/MERS [26, 27] . To overcome this problem of preexisting antibody, several approaches have been developed [28] [29] [30] [31] [32] [33] [34] [35] . One approach was to increase the dose of viral vector, but high-dose injection increased its cytotoxicity. Another approach was to change the surface antigen of the adenovirus vector used for booster immunizations to a rare serotype to evade the preexisting antibody [28] [29] [30] [31] . However, the concern with this strategy is the difficulty of mass production of two types of recombinant viruses, although packaging of the adenoviral vector with polymer [32, 33] and/or administration of adenovirus through other routes, such as mucosal or nasal inoculation, may circumvent this problem Mean changes in body weight ± standard deviation are shown. Asterisks indicate the significance of differences between the PBS group and the spike protein group. ** p < 0.01. *** p < 0.001. Hashes indicate the significance of differences between the PBS group and the Ad5/MERS-spike protein group. # p < 0.05. (B) Histopathological examination of homologously or heterologously vaccinated mice (spike protein nanoparticles or Ad5/MERS-spike protein) and nonvaccinated controls (PBS and Ad5/GFP). Lungs were collected 5 days postinfection. (C) Hematoxylin and eosin-stained sections of lung were examined and scored by blinded observers (n = 3). Score 0: absence of lesions. Score 1: small or occasional subpleural foci. Score 2: thickened intraalveolar septa and subpleural fibrotic foci. Score 3: thickened continuous subpleural fibrous foci and intraalveolar septa [40] . Each group had n = 5 mice. [34, 35] . Furthermore, these methods are dependent on the experimental conditions or the researcher's skill. Therefore, to overcome this disadvantage but to take advantage of a viral vector vaccine, in this study we used a heterologous prime-boost vaccination strategy, in which Ad5/MERS was used for the first (priming) vaccination to activate the cellular immune response and then MERS spike protein nanoparticles was used for the second and third (first and second booster) vaccinations to activate the humoral immune response. As shown in Figs. 3 and 4 , this strategy induced both specific neutralizing antibody and Th1 cells against MERS spike protein, indicating that it could effectively trigger a cellular immune response by priming with Ad5/MERS and bypass the limitations of viral vectors by boosting with MERS spike protein nanoparticles.

A recent study analyzing patients who survived MERS virus infection demonstrated that survival depended on induction of both a cell-mediated immune response and neutralizing antibody [36] . The analysis showed a correlation between the severity of symptoms, the activation of T cells extracted from peripheral blood mononuclear cells, and the intensity of the antibody response. According to these data, patients with stronger activation of a MERS-specific CD8 + T cell response showed faster viral clearance and had relatively shorter exposure to the virus. In this regard, adenovirus vaccines, which are known to increase cellular immune responses via activation of CD8 + T cells [26, [37] [38] [39] , may be strategic candidates for a MERS vaccine. The results shown in Fig. 6 indicate that both the Ad5/MERS-spike protein group and the spike protein group were protected against MERS-CoV challenge, as indicated by smaller body weight loss and less severe lung pathology after MERS-CoV infection. However, the spike protein group did not induce a Th1 immune response, but rather a Th2 immune response including induction of neutralizing antibody. This imbalance of Th1/Th2 responses suggests that immunization with spike protein nanoparticles alone may provide short-term protection against virus infection but not long-term maintenance of a protective immune response.

Previously, formalin-inactivated respiratory syncytial virus (RSV) vaccine induced severe vaccine-enhanced respiratory disease (VERD) after natural RSV infection [41] . Therefore, our safety concern is that VERD, even during a long interval between vaccination and natural infection, may increase MERS-CoV because it is another respiratory virus [1] . It has also been shown that the induction of allergic and biased Th2 immune responses and infiltration of inflammatory cells in the airways and lungs are the main characteristics of VERD in RSV-inactivated vaccines [42, 43] . Our heterologous prime-boost immunization strategy with Ad5/MERS and spike protein nanoparticle clearly showed a balanced induction of Th1 and Th2 immune responses. Therefore, this vaccine strategy may be safe. However, it requires more detailed study.

Therefore, the heterologous vaccination strategy used in this study (Ad5/MERS prime and spike protein nanoparticles boost) is the method that would be most feasibly applied in the clinic, because it effectively uses the advantages of both a viral vector vaccine and a protein vaccine, concurrently inducing both Th1 and Th2 immune responses to induce protective immunity against MERS-CoV. 

Middle East respiratory syndrome coronavirus: another zoonotic betacoronavirus causing SARS-like disease

Dipeptidyl peptidase 4 distribution in the human respiratory tract

World Health Organization. Middle East respiratory syndrome coronavirus

Coronaviruses-drug discovery and therapeutic options

Structure of MERS-CoV spike receptor-binding domain complexed with human receptor DPP4

Purified coronavirus spike protein nanoparticles induce coronavirus neutralizing antibodies in mice

MERS-CoV spike protein: targets for vaccines and therapeutics

Middle East respiratory syndrome coronavirus spike protein delivered by modified vaccinia virus Ankara efficiently induces virus-neutralizing antibodies

Immunogenicity of an adenoviral-based Middle East respiratory syndrome coronavirus vaccine in BALB/c mice

Systemic and mucosal immunity in mice elicited by a single immunization with human adenovirus type 5 or 41 vector-based vaccines carrying the spike protein of Middle East respiratory syndrome coronavirus

Protective efficacy of recombinant modified vaccinia virus Ankara delivering Middle East respiratory syndrome coronavirus spike glycoprotein

ChAdOx1 and MVA based vaccine candidates against MERS-CoV elicit neutralising antibodies and cellular immune responses in mice

A truncated receptor-binding domain of MERS-CoV spike protein potently inhibits MERS-CoV infection and induces strong neutralizing antibody responses: implication for developing therapeutics and vaccines

Intranasal vaccination with recombinant receptor-binding domain of MERS-CoV spike protein induces much stronger local mucosal immune responses than subcutaneous immunization: implication for designing novel mucosal MERS vaccines

Optimization of antigen dose for a receptor-binding domain-based subunit vaccine against MERS coronavirus

Receptor-binding domain-based subunit vaccines against MERS-CoV

MERS-CoV spike nanoparticles protect mice from MERS-CoV infection

A synthetic consensus anti-spike protein DNA vaccine induces protective immunity against Middle East respiratory syndrome coronavirus in nonhuman primates

Evaluation of candidate vaccine approaches for MERS-CoV

Peptide vaccines against cancer, infectious diseases, and conception

Ethical guidelines for use of experimental animals in biomedical research

Rapid generation of a mouse model for Middle East respiratory syndrome

The paradigm of Thl and Th2 cytokines

Induction of antigen-specific cytotoxic T lymphocytes in humans by a malaria DNA vaccine

Adenoviral vectors persist in vivo and maintain activated CD8+ T cells: implications for their use as vaccines

CD8+ T cell responses following replication-defective adenovirus serotype 5 immunization are dependent on CD11c+ dendritic cells but show redundancy in their requirement for TLR and nucleotide-binding oligomerization domainlike receptor signaling

Adenoviral vector immunity: its implications and circumvention strategies

Replication-deficient human adenovirus type 35 vectors for gene transfer and vaccination: efficient human cell infection and bypass of preexisting adenovirus immunity

Immunogenicity of recombinant adenovirus serotype 35 vaccine in the presence of pre-existing anti-Ad5 immunity

Recent advances in the development of HIV-1 vaccines using replication incompetent adenovirus vectors

Hexon-chimaeric adenovirus serotype 5 vectors circumvent pre-existing antivector immunity

Adenovirus complexed with polyethylene glycol and cationic lipid is shielded from neutralizing antibodies in vitro

Poly [lacticglycolic] acid copolymer encapsulation of recombinant adenovirus reduces immunogenicity in vivo

Oral vaccination of mice with adenoviral vectors is not impaired by preexisting immunity to the vaccine carrier

Nasal delivery of an adenovirus-based vaccine bypasses pre-existing immunity to the vaccine carrier and improves the immune response in mice

Recovery from the Middle East respiratory syndrome is associated with antibody and T cell responses

Adenovirus vector-induced immune responses in nonhuman primates: responses to prime boost regimens

Cellular immune responses of healthy individuals to intradermal administration of an E1-E3-adenovirus gene transfer vector

Replicationdefective adenovirus serotype 5 vectors elicit durable cellular and humoral immune responses in nonhuman primates

Redox-active protein thioredoxin prevents proinflammatory cytokine-or bleomycin-induced lung injury

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Combined virus-like particle and fusion protein-encoding DNA vaccination of cotton rats induces protection against respiratory syncytial virus without causing vaccineenhanced disease

RSV vaccine-enhanced disease is orchestrated by the combined actions of distinct CD4 T cell subsets

The authors declare that they have no conflicts of interest.

Supplementary data associated with this article can be found, in the online version, at https://doi.org/10.1016/j.vaccine.2018.04. 082.