key: cord-0778296-0jw5ccs4
authors: Golde, William T.; Nfon, Charles K.; Toka, Felix N.
title: Immune evasion during foot‐and‐mouth disease virus infection of swine
date: 2008-09-19
journal: Immunol Rev
DOI: 10.1111/j.1600-065x.2008.00672.x
sha: 7e4b1bb22ffe0cbbb8c91592533f6aea369915f5
doc_id: 778296
cord_uid: 0jw5ccs4

Summary: The interface between successful pathogens and their hosts is often a tenuous balance. In acute viral infections, this balance involves induction and inhibition of innate responses. Foot‐and‐mouth disease virus (FMDV) is considered one of the most contagious viruses known and is characterized by rapid induction of clinical disease in cloven hoofed animals exposed to infection. Viral shedding is extensive before the equally rapid resolution of acute disease. This positive strand RNA virus is an extremely successful pathogen, due in part to the ability to interrupt the innate immune response. Previous reviews have described the inhibition of cellular innate responses in the infected cell both in vitro and in vivo. Here, we present a review of virus inhibition of cells that are a source of antiviral function in swine. Particularly in the case of dendritic cells and natural killer cells, the virus has evolved mechanisms to interrupt the normal function of these important mediators of innate function, even though these cells are not infected by the virus. Understanding how this virus subverts the innate response will provide valuable information for the development of rapidly acting biotherapeutics to use in response to an outbreak of FMDV.

Pathogenicity of infectious agents is determined by many factors bearing on transmission such as susceptibility and type of exposure of the host to the agent. In addition, transmission of disease is also dependent on virulence of the pathogen and whether other infectious disease processes are already ongoing in the exposed individual. The evolution of successful pathogens seems remarkable in the face of what we know about mammalian immune systems after decades of intense investigation. However, pathogens in general and viral pathogens in particular rapidly mutate and evolve to counteract the non-specific, innate responses of mammals and the highly specific adaptive immune response.

Upon infection, a virus is faced with the immune response of the host. Because viruses rely on the host for growth, survival, and transmission, they have evolved diverse methods to escape elimination by the host's immune system. Generally, this is referred to as immune evasion, and the simple reason for a virus to evade the host's protective mechanisms is to acquire time for replication and transmission of progeny to other cells or hosts or to hide from surveillance by the immune system in a latent state. A large body of literature now exists on the main mechanisms engaged by viruses to evade the immune system. Immune evasion can be achieved by the interruption of various host responses including the innate response, cellular responses, humoral responses, and inhibition of immune effector molecules (1) . The result of immune evasion is clinical disease or infection that is protracted over time.

Viral subversion of cellular immune responses is often associated with reducing the influence of the most efficient mechanism of virus clearance, CD8 1 cytotoxic T lymphocytes (CTLs). Eliminating the possibility of recognizing viral epitopes presented in context of major histocompatibility complex (MHC) class I molecules is common during infection by successful viral pathogens (2) (3) (4) (5) . To evade humoral immunity, viruses may undergo antigenic variation or encode proteins that induce polyclonal activation of B cells, leading to hyperproduction of immunoglobulins (Igs) not necessarily protective against the virus (6, 7) . Other viruses encode highly glycosylated envelope proteins such that the glycosylation can mask the neutralizing epitopes, thus rendering the antibody response ineffective (8, 9) . In some infections, viral proteins have been shown to inhibit complement-mediated killing of infected cells (10) or target effector molecules by encoding either homologous cytokines or cytokine receptors, having acquired such genes through modification or capturing of host cellular genes (11) (12) (13) .

Rodent models of infectious disease depend upon similarities in the pathogenic progression of infection with the modeled species, i.e. human, companion pet, livestock, etc. There are many good rodent models of disease including severe acute respiratory syndrome coronavirus (SARS-CoV) in the aged mouse (over 12 months) (14) and some requiring adaptation of viral strains to mice such as influenza. However, other mouse 'models' have been reported extensively where disease pathology does not have any relationship to the pathology in the host of interest. A clear example in livestock is vesicular stomatitis virus (VSV), which does not cause vesicles at all in mice but rather causes encephalitis. Contrarily, this virus gains its name from the natural disease pathology in cattle, horses, and swine, which is characterized by vesicular lesions on the lips, tongue, snout, and coronary bands of the feet (15) . Similarly, rodents are a poor model for foot-and-mouth disease virus (FMDV) infection, as infection of mice requires high doses of virus and there is no clinical vesicular disease.

Further, important gene loci such as Ig and T-cell receptor diverged after separation of rodent and livestock species during evolution (16) , thereby compromising translation of results in mice to the natural hosts. Here, we focus our discussion to one of the natural hosts of FMDV infection, swine.

The present review focuses on the evasion of early innate responses of swine to FMDV and the clearance of virus during infection of naive animals. The role of important innate cell populations has been a focus of recent research efforts, and many aspects of the response of these cell types have been studied. A comprehensive understanding of the innate response to viral infection still requires a great deal more investigation, as knowledge of the relationship of this virus and the infected cell is extensive but not yet comprehensive. By contrast, information on the immunopathology of FMDV in infected animals is sparse, though recently there has been more activity on this subject. Here, we endeavor to inform about current knowledge as well as identify areas lacking in information and understanding. Understanding FMDV immunopathology in vivo remains a very high priority, given an outbreak of FMDV in North America is anticipated to cause losses in the billions of dollars to the economies of Mexico, Canada, and the United States.

Understanding the immune processes mediating immune evasion is made more relevant by studying diseases in their natural hosts. In swine, viral infections that can be studied to more fully understand immune evasion in a natural host include pseudorabies virus (PrV). This virus belongs to the alphaherpesviridae subfamily of Herpersvirinae, the same subfamily as the human herpes simplex virus (HSV) and bovine herpes viruses (BoHV), and has many features in common with these viruses. It infects the central nervous system (CNS) and establishes latency. PrV induces apoptosis in the host inflammatory cells, while it inhibits apoptosis of the trigeminal ganglion neurons where it resides during the latency period (17) . While the genes involved in similar anti-apoptotic mechanisms in HSV-1 or -2 infection are known, in PrV the most likely candidates are the Us3 and LAT genes, which share RNA homology with anti-apoptotic molecules in muscle cells (18) .

The same virus exhibits another mechanism that also may affect viral immune evasion. Infected cells display viral glycoproteins on the plasma membrane, but in the presence of virusspecific antibodies, redistribution of viral glycoproteins on the cell surface occurs, which leads to their aggregation, capping, and finally shedding. Further, when infected monocytes are incubated with glycoprotein-specific antibodies, viral glycoproteins are endocytosed. In both cases, the result is no display of viral proteins on the cell surface membrane of infected cells, rendering such cells invisible to CTLs. This is particularly important for glycoproteins B and D (19, 20) . Likely, PrV subverts the antibody response phase of infection until it establishes latency. However, it is not known whether such a mechanism is operational in vivo.

African swine fever virus belongs to the Asfarviridae family and uses a different mechanism to evade immune surveillance. Within 10 h of infection, this virus induces alteration of the secretory pathway by interfering with the trans-Golgi network (TGN). This interference leads to a loss of TGN46, a protein responsible for maintenance of the morphology of the TGN (21) , and API, an adapter protein involved in sorting secretory bound proteins exiting the TGN. The obvious consequence of these viral mechanisms is to disrupt the translocation of MHC class I molecules to the cell membrane surface (22) so as to eliminate a crucial immunosurveillance component.

A somewhat novel mechanism of immune evasion is exhibited by porcine circovirus Type 2 (PCV2). It causes an ailment termed post-weaning multisystemic wasting syndrome in piglets aged 5-12 weeks and has tropism for cells of the monocytic lineage. PCV2 escapes into dendritic cells (DCs) or macrophages and shows no evidence of replication and neither does the virus kill the cells nor alter any of the activation markers on the cells harboring the virus. There is no cytokine profile modification (23) . Once the virus is released from the cells, it is infectious to other cells. Given that DCs circulate to perform their function, it is possible that the virus is kept and spread in the host through DCs and macrophages without detection by the immune effector cells such as natural killer (NK) cells and CTLs. Moreover, PCV2 causes depletion of NK cells, gdT cells, and B cells (24) . However, what is not clear is whether there is any level of viral antigen presentation during the period that DCs carry the virus.

Porcine respiratory and reproductive syndrome virus (PRRSV), an enveloped, positive, single-stranded RNA virus that is a member of the Arteriviridae family in the order Nidovirales (25, 26) , causes a persistent infection of respiratory and reproductive tracts of pigs. The virus targets the DCs and downregulates expression of MHC class I and II and costimulatory molecules such as CD80/86 (27) . Although this virus is capable of inducing the translocation of activated nuclear factor-kB (NF-kB) to the nucleus followed by transcription of some genes such as matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) (28) and interleukin-10 (IL-10) (29) , it fails to activate transcription of interferon (IFN) regulatory factor 3 (IRF3), an important IFNb transcription factor (30) . It does so by inhibiting the activation of IPS-1 within the RIG-I signaling pathway leading to diminished production of IFNb. The lack of this type 1 IFN leads to reduced DC-NK cell cross-talk. Together with the upregulation of IL-10 impairing function of T lymphocytes, the virus is allowed to persist.

PRRSV also appears to attack the antibody responses as well. An elegant report by Butler et al. (7) shows that this virus manipulates the immune system by inducing polyclonal B-cell activation. In an isolator piglet model, PRRSV causes immune dysregulation characterized by heavy lymphoid hyperplasia and hypergammaglobulinemia. The generalized polyclonal B-cell activation may produce autoantibodies to double-stranded (ds) DNA, Golgi glycoproteins, and other autoantigens, presumably leading to subversion of normal B-cell repertoire development. The likely consequence of this is a delay in the development of effective PRRSV-specific adaptive immunity. Mulupuri et al. (31) report the delayed appearance of antibodies against crucial PRRSV antigens such GP5, supporting this hypothesis.

FMDV induces vesicular lesions on the feet, mouth, tongue, and teets of susceptible species such as cattle and swine. FMDV is often referred to as the most contagious virus known and can spread very rapidly through naive herds. A positive strand RNA virus, FMDV rapidly mutates in the course of an outbreak of infection, creating quasi species within the broader serotype (32) . This virus also induces a highly acute infection, with clinical disease developing rapidly after exposure and a high level of viremia early in infection. Fever and viremia last only 1 or 2 days, and lesions normally resolve rapidly, in 7-10 days. In a small percentage of cases, a carrier state can develop lasting more than a few months, and this is often associated with exposure of vaccinated cattle to virulent virus (33, 34) .

The understanding of the host pathogen relationship between susceptible species and this virus is extensive on the cellular level. Receptors for the virus have been identified and are all in the av integrin family. In addition, in vitro, this virus can use heparin sulfate as a receptor (35) . The in vivo role of this latter interaction is not well understood (reviewed in 36) .

Viral proteins are encoded in a single open reading frame, and the polypeptide is translated and subsequently processed by viral encoded proteases. The leader protease self-cleaves and, among other activities, cleaves the elongation factor 4 (elf-4) of the cellular transcription complex. This arrests capdependent mRNA and cellular protein synthesis, and because the virus has an internal ribosome entry sight, the protein synthesis complexes are bound by the viral RNA genome. Infected cells therefore have mostly viral protein synthesis and little cellular proteins translated (37, 38) . This is likely to have a significant effect on inducing immune responses, as infected cells have been shown to lose class I MHC expression. With no new protein synthesis, these effects likely lead to no viral protein peptides getting loaded into newly synthesized MHC molecules via the endogenous pathway, and therefore, no induction of a CD8 1 T-cell response can occur. Data for CD8 1 T-cell responses in animals infected with FMDV is minimal, and no infected cell killing by CTLs has ever been reported.

Leader protease (Lpro) is the first protein to be translated from the viral genome, and its effect on type 1 IFN responses has received much attention. Like many viruses, FMDV is very sensitive to these cytokines (39) (40) (41) . The bulk of knowledge in this area has been obtained by using a serotype A virus derived from an infectious clone (A12) that has the leader protease removed (LLA12). Deletion of Lpro in the LLA12 virus results in a highly attenuated virus that fails to replicate in otherwise susceptible primary cell lines (39) . In addition, infection of animals with the LLA12 virus rarely causes viremia, and lesions are restricted to the inoculation sites, resulting in the rapid clearance of the virus before induction of neutralizing antibodies (42, 43) . However both the wildtype virus and the LLA12 replicate to almost the same degree in BHK-21 cells or in suckling mice (44) due to defects in type 1 IFN response machinery in these systems (45, 46) . Furthermore, supernatants from LLA12-infected porcine or bovine fibroblasts had a stronger type 1 IFN-mediated antiviral activity relative to those from wildtype infection (40) . These data indicate that Lpro contributes to the virulence of FMDV and plays a significant role in suppressing the innate immune response to this virus, permitting replication and transmission between susceptible species.

Further research has demonstrated that in addition to blocking type 1 IFN translation, Lpro also inhibits transcription of IFNb and the IFN-stimulated genes 2 0 5 0 olygoadenylate synthetase, dsRNA protein kinase, and MX1 (41) . NF-kB is a ubiquitous protein that regulates genes (including IFNb) responsible for innate and adaptive immunity. Inactive NF-kB is found in the cytoplasm coupled to an inhibitory protein, IkB. Viral infections and other stressors induce a signaling cascade via pathogen-associated molecular pattern receptors leading to the activation of NF-kB (47) . Activated NF-kB translocates to the nucleus, where it mediates gene transcription. Infection of BHK-21 cells with FMDV wildtype or LLA12 results in translocation of NF-kB into the nucleus (48) . However, at later stages of the infection, there is degradation of the nuclear NF-kB in wildtype infected cells but not in the LLA12 infected cells. The degradation of NF-kB correlates with translocation of FMDV Lpro into the nucleus. Furthermore, NF-kB degradation is observed when Lpro is introduced into the cells in the absence of other FMDV proteins (48) . Thus, degradation of NF-kB by FMDV Lpro might be another mechanism by which FMDV mediates the downregulation of inflammatory gene transcription and hence inhibition of innate immunity.

The 3C protease (3Cpro) of picornaviruses is responsible for the cleavage of the viral polyproteins. FMDV 3Cpro has also been shown to not only cleave elF-4G but also elF-4A. However, 3Cpro accumulates at a slower rate than the Lpro, but the accumulation eventually exceeds the level required for processing viral polyprotein, and the excess can begin to cleave other substrates (49) . Based on the cleavage of elF-4G, it can be concluded that 3Cpro potentially exerts a similar immunosuppressive effect on infected cells as does the Lpro, albeit at late stages of infection.

FMDV Lpro, 3Cpro, and possibly other FMDV proteins (50) suppress transcription and/or translation of inflammatory cytokines in infected cells. Lysed cells harboring these proteins probably serve to supply inhibitory signals for cells such as DCs, macrophages, NK cells, and T cells, which are otherwise refractory for FMDV infection.

Some viral infections of swine cause immunopathology by inducing a decline in circulating lymphocytes. Classical swine fever virus, for example, induces a severe lymphopenia of 7-10 days duration (51) (52) (53) . PRRSV causes a transient leukopenia (54, 55) and pathology in lymphoid tissues of infected swine (56) . Similarly, multiple strains of FMDV have been shown to cause lymphopenia in swine involving multiple subsets of peripheral lymphocytes (57, 58, Nfon et al., manuscript submitted). This lymphopenia is characterized by a decline in the absolute numbers as well as percentage of lymphocytes in peripheral blood and closely correlates with peaks of viremia. The more virulent strains for pig (O1 Campos, O Taiwan 97, A24 Cruzeiro, C3 Resende, and Cs8C1) induce more profound lymphopenia compared with the less virulent strains (O-SK2000 and A12), inducing mild and delayed lymphopenia. Active infection of lymphocytes is not responsible for the lymphopenia, as virus could not be recovered from peripheral blood mononuclear cells (PBMCs) of infected pigs despite high viremia. In addition, PBMCs were not infected in vitro (59, 60) . However, a plaque purified, tissue culture strain of serotype C, Cs8C1, inoculated into young pigs has been reported to infect lymphocytes (58) . There is no significant increase in apoptosis of peripheral and tissue lymphocytes, and the recovery from lymphopenia is usually rapid.

We have consistently observed a transient serum IFNa response in FMDV-infected swine. Early after infection (48 h), all swine had detectable IFNa in serum regardless of virus serotype and strain used to infect. Peak levels of serum IFNa ranged from 200 to 1500 pg/ml on day 3 post-infection and dropped sharply to background levels by days 4-10 (Nfon et al., manuscript submitted). IFNa protects swine from FMDV infection when administered 24-48 h before challenge (61, 62) . IFNa may contribute to early clearance of viremia by preventing further propagation of virus in susceptible cells (42) . Despite this activity, FMDV replicates rapidly and attains peak viremia within 48-72 h of infection. This observation suggests a potential for swine to mount an innate response to FMDV that is insufficient to stop the early establishment of infection but plays a significant role in viral clearance. For instance, IFNa secretion may activate cells mediating an innate response and/or induce an antiviral state in susceptible tissue, although not in time to completely block infection. Either of these possibilities is consistent with published data analyzing exogenous delivery of IFNa (61) .

In pigs infected with CSFV, lymphopenia correlates with the levels of IFNa in serum (53) . Similarly, our observation of peak serum IFNa in porcine FMDV infection coincided with lowest lymphocyte numbers in blood. Thus, the serum IFNa response could partly account for the FMDV-associated lymphopenia in pigs. The connection between serum IFNa and lymphopenia has been clearly demonstrated in mice. Mice injected with poly I:C exhibit a lymphopenia linked to their serum IFNa levels (63) . Studies in gene-targeted mice show that infection with VSV is associated with lymphopenia, which is dependent on signaling through the type 1 IFN receptor (IFNaR) (64). Kamphuis et al. (64) , using adoptive transfer studies in mice with a B-or T-cell-specific IFNaR deletion, showed that IFNa/b exerted a direct effect on lymphocytes sufficient to induce lymphopenia.

At peak viremia and minimum lymphocyte numbers, the functional capacity of residual T cells is significantly reduced. These cells show little or no proliferation in response to stimulation with the mitogenic lectin concanavalin A (ConA) (57, 58) . In addition, they fail to secrete IFNg in response to ConA, and these functional deficiencies in T cells persist for up to 7 days post-infection, despite the rapid recovery in lymphocyte numbers by day 4. These rapid FMDV effects on T cells likely create a transient immunosuppressive state, further enabling the propagation and shedding of virus.

DCs are professional antigen-presenting cells, yet many DC subsets play a major role in innate responses to pathogens. These cells therefore are critical in regulating the early, nonspecific response to viral infection and transitioning to the induction of highly specific immune responses via activation of helper T cells if viral antigens are still present. DCs previously described in swine peripheral blood include myeloid/ monocyte-derived DCs (MoDCs) (65-67) and plasmacytoid DCs (pDCs) (68) . pDCs are specialized IFNa-secreting cells and produce large amounts of this cytokine in response to many viral infections. However, pDCs only respond to FMDV in the presence of immune serum (69) , suggesting that these cells might be less effective early in FMDV infections. MoDCs secrete type 1 IFNs in response to FMDV; however, stimulation of MoDCs with synthetic dsRNA, polyI:C (60), induces a stronger IFN response than FMDV.

We have previously reported the characterization of DCs isolated from porcine skin (70) . Recently, we have identified the majority of these cells as Langerhans cells (LCs) populating the epidermis (71) . Uniquely, porcine LCs constitutively express IFNa and secrete this cytokine after encountering FMDV in vitro (57, 60) . This finding may indicate a prominent role of LCs in FMDV infection.

IFNa secretion by MoDCs propagated from peripheral blood of animals infected with FMDV is transiently depressed during acute infection, specifically at 48 h post-infection (60) . In that study, we only analyzed cells harvested on days 2, 7, and 14 following infection as generation of the MoDC population for analysis required 7 days of in vitro propagation. MoDC secretion of IFNa recovered by day 7 to levels similar to preinfection samples. Subsequently, we analyzed PBMCs for pDC function by assessing the response to an innate immune stimulator. Daily analysis of PBMCs from FMDV-infected pigs revealed there is a transient decline in the number of IFNa-producing cells when stimulated with CpG 2216, a Toll-like receptor 9 (TLR-9) agonist. In addition, the IFNa in supernatants of these cultures is significantly lower on days 2-4 post-infection (Nfon et al., manuscript submitted).

LCs harvested from FMDV-infected pigs secrete little or no IFNa in response to ex vivo stimulation. The suppression of IFNa secretion of LCs following infection is more protracted, beginning on day 2 post-infection and lasting beyond 35 days. This timing apparently coincides with the turnover period for LCs, suggesting those LCs that have encountered viruses are permanently anergized for IFNa secretion (60) . The ability to make IFNa is a function of repopulation of the skin with naive LCs. Inhibition of type 1 IFN response of DCs is a feature of other viral infections (72) (73) (74) (75) (76) (77) (78) . During dengue virus infection, pDCs, though not productively infected, decline in number and secrete less type 1 IFN (77) . Similar effects on pDCs are observed in primary human immunodeficiency virus infections (76) . Furthermore, dermal DCs in warts caused by human papilloma virus fail to respond to imiquimod, a TLR-7 ligand (72), and vaccinia virus suppresses the ability of a LC line to secrete proinflammatory cytokines in response to stimulation with lipopolysaccharide or poly I:C (75) . Chronic hepatitis C virus affects both pDCs and MoDCs, causing these cells to secrete less cytokine in response to TLR ligands (73) .

The mechanisms by which FMDV suppresses DC IFN responses are not clearly understood. No live virus was isolated from monocytes (including DCs and macrophages) of FMDV-infected swine, despite the presence of high titers of virus in blood. Furthermore, immune serum is required for an abortive infection of monocytes in vitro (79) . Similarly, we found no evidence of productive infection of LCs in vitro and from LCs isolated from infected swine despite the high susceptibility of surrounding keratinocytes (59, 60) . Viral products and replication intermediates released by lysed, infected cells and taken up by DCs may account for this immunosuppression. However, viral RNA can be detected by polymerase chain reaction (PCR) of RNA samples isolated from in vitro exposed LCs after 1 h adsorption, indicating that FMDV can bind to and be internalized by LCs (59) . Indeed, by immunohistochemistry, Gregg et al. (80) detected low levels of FMDV antigen within LCs. Because FMDV is a positive sense RNA virus, viral protein synthesis can be initiated upon uptake and before virus inactivation. Thus, low levels of viral proteins, particularly the rapidly translated, self-cleaving Lpro, may occur within DCs even in the absence of replication.

Cellular responses are critical to clearance of the invading virus and are necessary for rapid response should reinfection occur. NK cells do not require expansion like the cells mediating antigen-specific adaptive responses, T cells and B cells, so there is no delay in NK-mediated responses. NK cells are able to recognize cells that are infected by a virus, usually via cell stress signals, and are capable of eliciting spontaneous cytotoxicity. These functions are regulated by NK cell-activating cytokines such as IL-2, IL-12, IL-15, IL-18, and IFNs (81) . Apart from cytokines, NK cells express activating receptors, such as NKp30, NKp44, and NKp46, in humans. Binding of these receptors signals activation of the NK cell. Alternatively, inhibiting receptors such as killer-cell immunoglobulin-like receptor and CD94-NKG2A (82) transmit inhibitory signals.

NK cells may be targets for viral subversion of immunity. Most studies on how NK cells are affected by infection have been performed on viruses that are persistent or chronic in nature, although acute infections may also lead to dysfunction of NK cells (reviewed in 83). In fact, during porcine infection with FMDV, this may be the case. Pig peripheral blood NK cells are rather quiescent in nature and only increase their cytotoxicity after stimulation with NK cell-activating cytokines, such as IL-15 (84, Toka et al., manuscript submitted). Peripheral blood NK cells from FMDV serotype O-infected pigs do not increase their cytotoxicity, as opposed to the responses of these cells in infections such as MCVM in the mouse (85, 86) . Instead, the swine NK cell response declines beginning from the second or third day after FMDV infection. Such a state of dysfunction usually lasts for 2-3 days, after which reactivity returns to background levels. Higher viral titers in the serum of infected animals appear to coincide with the reduction in NK cell cytotoxicity. Curiously, when the effector protein expression such as perforin was compared in infected cells and non-infected cells, there was only a marginal difference (Toka et al., manuscript submitted). This observation may indicate that while the granzyme/perforin killing mechanism may be intact, other pathways are possibly modulated by FMDV resulting in reduced antiviral activity of NK cells. However, intracellular expression of IFNg was inhibited, which may largely contribute the inactive status of NK cells during FMDV infection. Inhibition of cytokines or chemokines is one of the strategies used by viruses to subvert the immune system. These processes take place in non-infected cells, requiring that the mechanism of this inhibition likely involves products, cellular or viral, from infected cells.

Some viruses encode proteins that interact with NK cells, and during this interaction, NK cells are inhibited in their primary function. Such is the case when CD81 directly binds E2 of hepatitis C virus (87) . If such interaction occurs early in infection, the costimulatory effect of NK cells on DCs will be hampered, and as a result, DC-dependent activation of NK cell lytic activity. Commonly, NK cells recognize infected cells by virally induced lack of MHC expression; however, some viral proteins may upregulate expression of MHC class I molecules in infected cells, inhibiting NK function. Furthermore, viruses can interrupt NK receptor-mediated recognition of cells infected with virus by blocking cell-activating cytokines such as IFNa, IL-12, IL-15, and IL-18.

Although no evidence is yet available, the inhibition of activating receptors on NK cells may be involved in FMDV infection of swine. Indeed, in vitro experiments with NK cells isolated from pigs infected with FMDV do not exhibit significant levels of cytotoxicity. Measurement of NK cell expression of activating receptors shows a rather unaltered profile. Of the three NK cell receptors (NCRs), only NCR3 is upregulated, at least in the first 3 days after infection, followed by GZMB, KLRC1, and KLRA1. The remaining genes, such as NCR1, KLRF1, GZMA, KLRB1, KLRK1, SH2DIB, and PRF1 are not upregulated, suggesting that there may be selective inhibition of expression of these genes during infection with FMDV (Toka et al., manuscript submitted).

In other virus systems, inhibition of activating receptor function has been attributed to downregulation of NCR ligands, mainly on virus-infected cells. For instance, in human cytomegalovirus-infected cells, there is downregulation of leukocyte function-associated antigen-3, which renders these cells refractory to killing by NK cells (88) . However, modulation of NK cell function by infection of NK cells has also been reported for vaccinia virus (89) . Apparently, infection modulates the signaling in NK cells such that the cells become more sensitive to inhibitory signals likely derived from target cells.

As reviewed above, FMDV does not infect lymphocytes in vivo (57) . Therefore, inhibition in terms of activating receptor manipulation by the virus may be a bystander effect exerted by viral protein products or simply binding of the virus particles to porcine NK cells. It remains to be determined what viral proteins might possibly be involved. Conversely, this inhibition may take the form of antagonism of the cytokines that regulate inflammatory and immune responses, often targets of subversion by viruses.

A few examples are Epstein-Barr virus, which encodes IL-10, and vIL-10 negatively regulates IL-12 by various leukocytes and subsequently IFNg production by NK cells. Adenoviruses have evolved to express proteins that neutralize TNFa, and poxviruses encode soluble cytokine receptors able to block the most important regulators of immune and inflammatory responses such as IFNg, IFNab, TNF, and IL-1 (90) . Although there is no evidence of FMDV encoding cytokine and cytokine receptor homologues, it is clear that NK cells derived from FMDV-infected pigs are unable to secrete IFNg (Toka et al., manuscript submitted). The mechanism for inhibition is currently difficult to discern. But likely it may be through a virus protein cross-linking to NK cell inhibiting receptor, or the situation may be similar to that shown for measles virus, where virus binding to CD46 on monocytes leads to immunosuppression of cell-mediated responses (91) .

Although IFNa secretion by DCs is blocked during acute FMDV infection of swine (60) , the molecules required for adequate antigen-presenting function of DCs, MHC class II and CD80/ 86, are unaffected. Similarly, the ability of DCs to take up particulate matter and process protein antigens is undiminished by FMDV (59, 60) . This finding suggests that these DCs retain the ability to process and present FMDV antigen, thus initiating the strong antibody response extensively reported in FMDV infection of swine (36) . FMDV has evolved to establish acute infections characterized by rapid replication and spread between susceptible animals. Persistent infections are rare in swine. Suppression of innate function of DCs by FMDV creates a window for viral replication and shedding before the onset of an adaptive immune response.

The anti-FMDV antibody response supports the hypothesis that antigen presentation function remains intact. Various analyses of the quality of the antibody response in swine indicate that there is a broad induction of CD4 1 helper T cells that produce B-cell-activating cytokines. Anti-FMDV antibody of the IgM isotype is detected as early as 4-7 days, peaking by 10 days and waning by 14-21 days after infection. Using polyclonal anti-swine IgG antiserum, the IgG response is detectable around 14 days after infection and lasts for months. There are few reagents available to distinguish the six IgG isotypes of swine Ig, and the genetics of this locus indicate allelic differences will also be problematic to dissect using antibodies specific for these isotypes, if they were available.

In other species, the induction of particular isotypes of antibody is associated with the T-helper type 1 (Th1)/Th2 paradigm. These subsets of CD4 1 , class II MHC-restricted T cells respond to activation by antigen-presenting cells and antigen by producing unique patterns of cytokine secretion. Th1 is characterized in all species by production of IFNg, and Th2 cells are a prominent source of IL4, IL-5, and IL-13 following stimulation. In swine, analysis of the association of specific Ig responses with Th1 or Th2 T cells is not possible, given only two antibodies are presently available, anti-IgG1 and anti-IgG2. However, we have previously reported a reduced T-cell proliferation and lack of IFNg secretion in response to mitogen during acute FMDV infection (57) . Interestingly, when the antibody response to FMDV was analyzed using these limited reagents, we detected a putative IgG1 antibody reactivity with FMDV but no IgG2 antiviral reactivity. Porcine IgG2 antibody reactivity with a standard protein antigen, chicken ovalbumin, was readily detected (W. Golde, unpublished data). This highly preliminary data may indicate an imbalance in the Th1/Th2 response during FMDV infection.

Infected cell expression of MHC proteins loaded with virally derived peptides is the hallmark of cellular immunity mediated by CTLs. This phenomenon allows detection and elimination of cells that are infected with virus. Class I MHC-restricted CD8 1 T-cell responses are diminished to absent in FMD-infected animals. Viruses have evolved several mechanisms to interrupt this critical infected cell labeling system.

The most common and well studied viral subversion strategies of MHC class I include inhibition of peptide translocation to endoplasmic reticulum, interference with cytosolic proteolysis resulting in inhibition of processing of antigenic peptides to be loaded into class I heterodimers, or retention and destruction of MHC class I. These effects hinder the presentation of viral antigens leading to non-recognition of infected cells by CTLs (78, (92) (93) (94) . When assembly of the MHC molecules loaded with specific peptides is complete, they are delivered to the cell membrane surface, but viral proteins including the US3 protein of HCMV (95) and 2B and 3A proteins of poliovirus (96) can delay surface expression of these complexes. Even when the MHC-peptide complexes finally arrive at the cell membrane surface, viral proteins, such as B3 proteins of coxsackievirus (97) and Kaposi's sarcoma-associated herpes virus gene product K3 (98) , can disguise these molecules.

Infection of porcine fibroblast cell lines with FMDV led to reduction of surface MHC class I molecules expression of approximately 50% by 10 h post-infection. On the contrary, swine alveolar macrophages incubated with FMDV increased the expression of both MHC class I and II molecules within the same time frame. Following acid treatment of the fibroblast cell lines infected with FMDV, there was no rapid reappearance of MHC class I molecules, indicating that there was inhibition of assembly of new molecules (99) . The mechanism of reduction of MHC class I expression on infected fibroblasts could be that described by Moffat et al. (50) . Using TsO45 mutant of VSV G protein to track movement of proteins from the endoplasmic reticulum to the cell surface membrane, they showed that cotransfection of cells with FMDV 2BC and TsO45-GYFP led to inhibition of delivery of the G-protein to cell surface. Therefore, it is possible that FMDV uses this strategy to inhibit the secretory pathway leading to retention of proteins such MHC class I molecules and cytokines that are necessary to induce an effective immune response against FMDV in swine.

The analysis of T-cell responses to FMDV antigens in cattle and swine is very limited. Attempts to identify peptide specificities have been difficult to quantify. A more sophisticated understanding of immune evasion of T-cell responses will require more basic analysis of T-cell responses to this virus.

The data reviewed here describe a very delicate balance between immunosuppression by FMDV and immune response by the host, which more often favors the virus in the acute phase of infection. Eventually, innate and ultimately adaptive responses favor the host, not only in the clearance of virus and recovery from clinical disease but also in protection from reinfection. Given that protection; is a serum antibody specific, the very definition of serotypes, such protection is mediated by antibody. There appears to be little contribution of the cellular response mediated by CD8 1 T cells acting as CTLs. CD8 1 T cells producing IFNg is a more common observation but still poorly understood.

Previous reviews have elegantly summarized the virus life cycle in infected cells, cellular receptors for the virus, clinical progression of disease, and analysis of outbreaks of note. Here, we endeavored to focus on the innate response to this highly acute infection. In general, it would seem that such robust antiviral responses should protect at least swine from infection. However, we summarize here the subversion of many cells that mediate innate responses, especially DCs and NK cells. This subversion and that of other cells yet to be evaluated appear to be just sufficient to create the opportunity for the virus to replicate and spread to the next individual. The highly contagious nature of this picornavirus, therefore, is another critical factor in the success of this pathogen.

The innate response to this virus is a ripe target for creating new innovative approaches to develop rapidly acting biotherapeutics. Continued and more detailed analysis of the interaction of this virus with susceptible hosts will provide the information necessary to combat outbreaks and limit the devastation they can cause.

Viral immune evasion: a masterpiece of evolution

An early pseudorabies virus protein down-regulates porcine MHC class I expression by inhibition of transporter associated with antigen processing (TAP)

Human cytomegalovirus protein US2 interferes with the expression of human HFE, a nonclassical class I major histocompatibility complex molecule that regulates iron homeostasis

Cowpox virus exploits the

Cowpox virus evades CTL recognition and inhibits the intracellular transport of MHC class I molecules

Antibody repertoire development in fetal and neonatal piglets: XIX. Undiversified B cells with hydrophobic HCDR3s preferentially proliferate in the porcine reproductive and respiratory syndrome

Porcine reproductive and respiratory syndrome virus subverts repertoire development by proliferation of germline-encoded B cells of all isotypes bearing hydrophobic heavy chain CDR3

The neutralizing activity of antihepatitis C virus antibodies is modulated by specific glycans on the E2 envelope protein

The use of monoclonal antibodies and lectins to identify changes in viral glycoproteins that are influenced by glycosylation: the case of human respiratory syncytial virus attachment (G) glycoprotein

Inhibition of complementmediated cytolysis by the terminal complement inhibitor of herpesvirus saimiri

Virus-encoded modulators of cytokines and growth factors

Virus-encoded receptors for cytokines and chemokines

Virus encoded cytokines and cytokine receptors

Animal models and vaccines for SARS-CoV infection

Emergence and re-emergence of vesicular stomatitis in the United States

The isolator piglet: a model for studying the development of adaptive immunity

Induction and inhibition of apoptosis by pseudorabies virus in the trigeminal ganglion during acute infection of swine

Expression of the pseudorabies virus latency-associated transcript gene during productive infection of cultured cells

Antibody-induced endocytosis of viral glycoproteins and major histocompatibility complex class I on pseudorabies virusinfected monocytes

Entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages via receptor-mediated endocytosis

The trans Golgi network is lost from cells infected with African swine fever virus

African swine fever virus causes microtubule-dependent dispersal of the trans-golgi network and slows delivery of membrane protein to the plasma membrane

Dendritic cells harbor infectious porcine circovirus type 2 in the absence of apparent cell modulation or replication of the virus

Association of lymphopenia with porcine circovirus type 2 induced postweaning multisystemic wasting syndrome (PMWS)

Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the arterivirus group

Molecular characterization of positive-strand RNA viruses: pestiviruses and the porcine reproductive and respiratory syndrome virus (PRRSV)

Porcine reproductive and respiratory syndrome virus productively infects monocyte-derived dendritic cells and compromises their antigen-presenting ability

Porcine arterivirus activates the NF-[kappa]B pathway through I[kappa]B degradation

Porcine reproductive and respiratory syndrome virus infects mature porcine dendritic cells and up-regulates interleukin-10 production

Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses interferon-[beta] production by interfering with the RIG-I signaling pathway

Antigen-specific B-cell responses to porcine reproductive and respiratory syndrome virus infection

Foot-and-mouth disease virus evolution: exploring pathways towards virus extinction

Emergency vaccination against foot-and-mouth disease: rate of development of immunity and its implications for the carrier state

Vaccination against foot-andmouth disease virus confers complete clinical protection in 7 days and partial protection in 4 days: use in emergency outbreak response

Efficient infection of cells in culture by type O foot-and-mouth disease virus requires binding to cell surface heparan sulfate

Foot-and-mouth disease

Leader protein of footand-mouth disease virus is required for cleavage of the p220 component of the capbinding protein complex

The two species of the foot-andmouth disease virus leader protein, expressed individually, exhibit the same activities

Inhibition of L-deleted foot-and-mouth disease virus replication by alpha/beta interferon involves double-stranded RNA-dependent protein kinase

Ability of foot-and-mouth disease virus to form plaques in cell culture is associated with suppression of alpha/beta interferon

The leader proteinase of footand-mouth disease virus inhibits the induction of beta interferon mRNA and blocks the host innate immune response

Pathogenesis of wild-type and leaderless foot-and-mouth disease virus in cattle

Protection of swine by live and inactivated vaccines prepared from a leader proteinasedeficient serotype A12 foot-and-mouth disease virus

The foot-and-mouth disease virus leader proteinase gene is not required for viral replication

Foot-andmouth disease virus: the role of the leader proteinase in viral pathogenesis

Alpha/beta interferon protects adult mice from fatal Sindbis virus infection and is an important determinant of cell and tissue tropism

Innate immune recognition

Degradation of nuclear factor kappa B during foot-and-mouth disease virus infection

Foot-and-mouth disease virus 3C protease induces cleavage of translation initiation factors eIF4A and eIF4G within infected cells

Effects of foot-and-mouth disease virus nonstructural proteins on the structure and function of the early secretory pathway: 2BC but not 3A blocks endoplasmic reticulum-to-Golgi transport

Lymphocyte apoptosis during classical swine fever: implication of activation-induced cell death

Depletion of CD4(1) and CD8(high1) T-cells before the onset of viraemia during classical swine fever

High IFN-alpha responses associated with depletion of lymphocytes and natural IFN-producing cells during classical swine fever

Experimental porcine reproductive and respiratory syndrome virus infection in one-, four-, and 10-week-old pigs

Hematological and immunological parameters of 4 1/2-month old pigs infected with PRRS virus

Pathogenesis of porcine reproductive and respiratory syndrome virus infection in gnotobiotic pigs

Induction of lymphopenia and inhibition of T cell function during acute infection of swine with foot and mouth disease virus (FMDV)

Selective lymphocyte depletion during the early stage of the immune response to footand-mouth disease virus infection in swine

Constitutive expression of alpha interferon by skin dendritic cells confers resistance to infection by footand-mouth disease virus

Interferon-alpha production by swine dendritic cells is inhibited during acute infection with foot-and-mouth disease virus

Novel viral disease control strategy: adenovirus expressing alpha interferon rapidly protects swine from foot-and-mouth disease

Immediate protection of swine from foot-and-mouth disease: a combination of adenoviruses expressing interferon alpha and a foot-and-mouth disease virus subunit vaccine

Depletion of T cells by type I interferon: differences between young and aged mice

Type I interferons directly regulate lymphocyte recirculation and cause transient blood lymphopenia

IL-13 replaces IL-4 in development of monocyte derived dendritic cells (MoDC) of swine

Porcine dendritic cells generated in vitro: morphological, phenotypic and functional properties

Characterisation of porcine monocyte-derived dendritic cells according to their cytokine profile

Type-A CpG oligonucleotides activate exclusively porcine natural interferon-producing cells to secrete interferon-alpha, tumour necrosis factor-alpha and interleukin-12

Plasmacytoid dendritic cell activation by foot-andmouth disease virus requires immune complexes

Characterization and functional analysis of skinderived dendritic cells from swine without a requirement for in vitro propagation

Langerhans cells in porcine skin

Dermal dendritic cells in anogenital warty lesions unresponsive to an immune-response modifier

Differential dysfunction in dendritic cell subsets during chronic HCV infection

Severe herpes virus (HSV-2) infection in two patients with myelodysplasia and undetectable NK cells and plasmacytoid dendritic cells in the blood

Vaccinia virus infection attenuates innate immune responses and antigen presentation by epidermal dendritic cells

Type I interferon production is profoundly and transiently impaired in primary HIV-1 infection

A blunted blood plasmacytoid dendritic cell response to an acute systemic viral infection is associated with increased disease severity

Structural and functional dissection of the human cytomegalovirus immune evasion protein US6

Foot-and-mouth disease virus undergoes restricted replication in macrophage cell cultures following Fc receptor-mediated adsorption

African swine fever virus infection of skin-derived dendritic cells in vitro causes interference with subsequent foot-and-mouth disease virus infection

Natural killer cells in antiviral defense: function and regulation by innate cytokines

Anti-immunology: evasion of the host immune system by bacterial and viral pathogens

Synergistic effects of IL-2, IL-12 and IL-18 on cytolytic activity, perforin expression and IFN-gamma production of porcine natural killer cells

Direct recognition of cytomegalovirus by activating and inhibitory NK cell receptors

MCMV glycoprotein gp40 confers virus resistance to CD81T cells and NK cells in vivo

Inhibition of natural killer cells through engagement of CD81 by the major hepatitis C virus envelope protein

Natural killer cell lysis of cytomegalovirus (CMV)-infected cells correlates with virally induced changes in cell surface lymphocyte functionassociated antigen-3 (LFA-3) expression and not with the CMV-induced down-regulation of cell surface class I HLA

Vaccinia virus modulation of natural killer cell function by direct infection

Inhibition of TNF receptor 1 internalization by adenovirus 14.7 K as a novel immune escape mechanism

Mechanism of suppression of cell-mediated immunity by measles virus

Cytomegalovirus selectively blocks antigen processing and presentation of its immediate-early gene product

Inhibition of ubiquitin/proteasome-dependent protein degradation by the Gly-Ala repeat domain of the Epstein-Barr virus nuclear antigen 1

The varicellovirus-encoded TAP inhibitor UL49.5 regulates the presentation of CTL epitopes by Qa-1b1

Human cytomegalovirus inhibits tapasin-dependent peptide loading and optimization of the MHC class I peptide cargo for immune evasion

Inhibition of cellular protein secretion by poliovirus proteins 2B and 3A

Coxsackievirus B3 proteins directionally complement each other to downregulate surface major histocompatibility complex class I

Ubiquitylation of MHC class I by the K3 viral protein signals internalization and TSG101-dependent degradation

Infection with foot-and-mouth disease virus results in a rapid reduction of MHC class I surface expression

We acknowledge our colleagues and collaborators on the work conducted at Plum Island Animal Disease Center (PIADC). In particular, we thank Dr Elida Bautista, Boerhinger Ingleheim Animal Health, who initiated the DC work, Mr Geoffery Ferman, Department of Safety, PIADC,